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The map label for this gene is 119944733

Identifier: 119944733

GI number: 119944733

Start: 1222991

End: 1224628

Strand: Direct

Name: 119944733

Synonym: Ping_0974

Alternate gene names: NA

Gene position: 1222991-1224628 (Clockwise)

Preceding gene: 119944732

Following gene: 119944735

Centisome position: 26.82

GC content: 44.14

Gene sequence:

>1638_bases
ATGTCGTTATTCTCAATTATTAAAGACTGTGGTGGTATTCATAATATCCAGCGTGTTCTTATCCCTGATTCGCGTATTAT
TATTGAGATTCATGAGTTTGAATTGTTATCTGCAGAGGCAAAACAACAAGCTTATAATTTAACTCTAAAGCAGGTGACTT
ATCAGCAAACTGTTATTGAAAATAAAGATGAATTTGGGGCGCAACTATTAGAGATTGGCCAGCTAATCAGTGATCAACAG
CGGCAGGACATTACGCCTTATACAGCGCCCGTTGAATGTGAGTTTCGTCCCGACTGGCATATCTCGCCCCCACAAGGTTT
GTTAAACGATCCGAATGGTTTTATTTATCACCAAGGACAATATCACCTTTTTTATCAGTGGTACCCCTATACTTGTGTAC
ACAAAGATAAGTATTGGGCGCATTTAACCAGCAAAGATTTAGTGAACTGGCAGTGGCAACCAGTTGCTTTAACGCCCTCG
GACTGGTTTGACAGCTACGGTGTTTTTTCAGGTCACGCGATTAGTCAGGATGATCTGTTAATGTTGTTTTATACCGGCAA
TGTGCGTATTGGTGAGCAGCGAGACAGACACACCACCCAATGTTTGGCCACTTCAACGGATGGTATTCACTTTACTAAAC
AGGGCCCGGTTGTGCCTGAATTGCCACCGGGGGTGACACCGCATTGCCGCGATCCCAAAGTTATCCGTCATAATGACCGT
TGGTTAATGTTGCTGGGTGTACAGCGTGAAGATGAAATTGGCCGTTTAGCGATTTACCACTCGGAGGATCTCAAAACCTG
GACATTTATTGCCCTGTGTGGTGACGAGTTGGGTGATTTTGGTTATATGTGGGAATGCCCTGATTTTTTCACCTTAAACC
AGCAAGACTTTATTGTTATTGGCCCGCAGGGCATTAGATCGCCTGATAAGAGCCATACCGCCCCGCATCACAACGGTATT
GTCAAAGCGCAACTGGAGACAAGCGGCAAAGCCCTGTTATCTGATTTTCAACCTTTAGATTATGGTTTTGATTTTTATGC
CCCGCAATCATTAGAGACCCCCGACGGGCGAAGAATTATGTGTGCCTGGATGGGGTTGCCCGATGAAATCGATCATCCGA
GCGCCGACAATGGTTGGGTACATCAATTAACCACAATGCGGGAATTATCCTATGACAATGGTGCGTTAATCCAAAAACCC
ATTAAAGAGTTGGCAACGCTTCGTCATACGCCGATTGTGCTATCGAAAGATGAAACTTCTTTTGATTTGCACAGTAAAGC
ATTTGAGCTGCAAGTTTCAATGCAGTGGGGCAGTGTTTTACGTTTACACCAGTCTGAGTCCGGTTATTGTGAAATTCGTC
TAGATTCAGCATCGCGTCGATTATATATTGATCGCAGTAATACCCTGATACGCGAAGGAGATACCGTGCGTGAAATGTCT
TTGGCCGAATCTGATTCGGTTCAGCTGCACATATTCTCTGATACTTCGTCATTAGAGGTTTTTATTAATGAGGGGGAAGC
GGTAATGTCAGCGCGTGTGTTTACCGATAAGAATTCCACACAGTTGAGCTTCGATGGAGATGTGCAGATACAGGCCTGCT
GGTTATTAAATAAAGCAAGCGCTCCCTTCATATCTTAA

Upstream 100 bases:

>100_bases
TAAATAAAAAATCATCTTATTGCAAATATGCCTTTATTTTTATATTCTGCAAACGTTCCCATTTATATTTATACTTTTTA
TGAATTAATACAGGTGTTAT

Downstream 100 bases:

>100_bases
AACACTTAATTTAAATGAGAATTAAAACGAGGATTTAATCATTCACTAAAAGGATTAAATTCTCGTTAAAATGCACCTGG
AAAAGTTATTTTAATTTTTT

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSLFSIIKDCGGIHNIQRVLIPDSRIIIEIHEFELLSAEAKQQAYNLTLKQVTYQQTVIENKDEFGAQLLEIGQLISDQQ
RQDITPYTAPVECEFRPDWHISPPQGLLNDPNGFIYHQGQYHLFYQWYPYTCVHKDKYWAHLTSKDLVNWQWQPVALTPS
DWFDSYGVFSGHAISQDDLLMLFYTGNVRIGEQRDRHTTQCLATSTDGIHFTKQGPVVPELPPGVTPHCRDPKVIRHNDR
WLMLLGVQREDEIGRLAIYHSEDLKTWTFIALCGDELGDFGYMWECPDFFTLNQQDFIVIGPQGIRSPDKSHTAPHHNGI
VKAQLETSGKALLSDFQPLDYGFDFYAPQSLETPDGRRIMCAWMGLPDEIDHPSADNGWVHQLTTMRELSYDNGALIQKP
IKELATLRHTPIVLSKDETSFDLHSKAFELQVSMQWGSVLRLHQSESGYCEIRLDSASRRLYIDRSNTLIREGDTVREMS
LAESDSVQLHIFSDTSSLEVFINEGEAVMSARVFTDKNSTQLSFDGDVQIQACWLLNKASAPFIS

Sequences:

>Translated_545_residues
MSLFSIIKDCGGIHNIQRVLIPDSRIIIEIHEFELLSAEAKQQAYNLTLKQVTYQQTVIENKDEFGAQLLEIGQLISDQQ
RQDITPYTAPVECEFRPDWHISPPQGLLNDPNGFIYHQGQYHLFYQWYPYTCVHKDKYWAHLTSKDLVNWQWQPVALTPS
DWFDSYGVFSGHAISQDDLLMLFYTGNVRIGEQRDRHTTQCLATSTDGIHFTKQGPVVPELPPGVTPHCRDPKVIRHNDR
WLMLLGVQREDEIGRLAIYHSEDLKTWTFIALCGDELGDFGYMWECPDFFTLNQQDFIVIGPQGIRSPDKSHTAPHHNGI
VKAQLETSGKALLSDFQPLDYGFDFYAPQSLETPDGRRIMCAWMGLPDEIDHPSADNGWVHQLTTMRELSYDNGALIQKP
IKELATLRHTPIVLSKDETSFDLHSKAFELQVSMQWGSVLRLHQSESGYCEIRLDSASRRLYIDRSNTLIREGDTVREMS
LAESDSVQLHIFSDTSSLEVFINEGEAVMSARVFTDKNSTQLSFDGDVQIQACWLLNKASAPFIS
>Mature_544_residues
SLFSIIKDCGGIHNIQRVLIPDSRIIIEIHEFELLSAEAKQQAYNLTLKQVTYQQTVIENKDEFGAQLLEIGQLISDQQR
QDITPYTAPVECEFRPDWHISPPQGLLNDPNGFIYHQGQYHLFYQWYPYTCVHKDKYWAHLTSKDLVNWQWQPVALTPSD
WFDSYGVFSGHAISQDDLLMLFYTGNVRIGEQRDRHTTQCLATSTDGIHFTKQGPVVPELPPGVTPHCRDPKVIRHNDRW
LMLLGVQREDEIGRLAIYHSEDLKTWTFIALCGDELGDFGYMWECPDFFTLNQQDFIVIGPQGIRSPDKSHTAPHHNGIV
KAQLETSGKALLSDFQPLDYGFDFYAPQSLETPDGRRIMCAWMGLPDEIDHPSADNGWVHQLTTMRELSYDNGALIQKPI
KELATLRHTPIVLSKDETSFDLHSKAFELQVSMQWGSVLRLHQSESGYCEIRLDSASRRLYIDRSNTLIREGDTVREMSL
AESDSVQLHIFSDTSSLEVFINEGEAVMSARVFTDKNSTQLSFDGDVQIQACWLLNKASAPFIS

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=350, Percent_Identity=27.7142857142857, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SCRB_PSYIN (A1STJ9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942413.1
- ProteinModelPortal:   A1STJ9
- SMR:   A1STJ9
- STRING:   A1STJ9
- GeneID:   4626528
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0974
- eggNOG:   COG1621
- HOGENOM:   HBG693902
- OMA:   DISSAEI
- PhylomeDB:   A1STJ9
- ProtClustDB:   CLSK788970
- BioCyc:   PING357804:PING_0974-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232
- SMART:   SM00640
- TIGRFAMs:   TIGR01322

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N; SSF49899 ConA_like_lec_gl

EC number: =3.2.1.26

Molecular weight: Translated: 62260; Mature: 62129

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: ACT_SITE 110-110 BINDING 126-126 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFSIIKDCGGIHNIQRVLIPDSRIIIEIHEFELLSAEAKQQAYNLTLKQVTYQQTVIE
CCHHHHHHHCCCCCCEEEEEECCCEEEEEEECEEEECHHHHHHHHEEEEHHEEHHHHHHC
NKDEFGAQLLEIGQLISDQQRQDITPYTAPVECEFRPDWHISPPQGLLNDPNGFIYHQGQ
CCHHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCC
YHLFYQWYPYTCVHKDKYWAHLTSKDLVNWQWQPVALTPSDWFDSYGVFSGHAISQDDLL
EEEEEEECCEEEEECCCEEEEECCCCCCCEEEEEEEECCHHHHCCCCCCCCCEECCCCEE
MLFYTGNVRIGEQRDRHTTQCLATSTDGIHFTKQGPVVPELPPGVTPHCRDPKVIRHNDR
EEEEECCEEECCCCCCCHHEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCC
WLMLLGVQREDEIGRLAIYHSEDLKTWTFIALCGDELGDFGYMWECPDFFTLNQQDFIVI
EEEEEECCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCEEEECCCEEEECCCCEEEE
GPQGIRSPDKSHTAPHHNGIVKAQLETSGKALLSDFQPLDYGFDFYAPQSLETPDGRRIM
CCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEE
CAWMGLPDEIDHPSADNGWVHQLTTMRELSYDNGALIQKPIKELATLRHTPIVLSKDETS
EEECCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCC
FDLHSKAFELQVSMQWGSVLRLHQSESGYCEIRLDSASRRLYIDRSNTLIREGDTVREMS
CEECCEEEEEEEEECCCCEEEEECCCCCEEEEEECCCCCEEEEECCCCEEECCCCCEEEC
LAESDSVQLHIFSDTSSLEVFINEGEAVMSARVFTDKNSTQLSFDGDVQIQACWLLNKAS
CCCCCCEEEEEEECCCCEEEEEECCCEEEEEEEEECCCCCEEEECCCEEEEEEEEEECCC
APFIS
CCCCC
>Mature Secondary Structure 
SLFSIIKDCGGIHNIQRVLIPDSRIIIEIHEFELLSAEAKQQAYNLTLKQVTYQQTVIE
CHHHHHHHCCCCCCEEEEEECCCEEEEEEECEEEECHHHHHHHHEEEEHHEEHHHHHHC
NKDEFGAQLLEIGQLISDQQRQDITPYTAPVECEFRPDWHISPPQGLLNDPNGFIYHQGQ
CCHHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCC
YHLFYQWYPYTCVHKDKYWAHLTSKDLVNWQWQPVALTPSDWFDSYGVFSGHAISQDDLL
EEEEEEECCEEEEECCCEEEEECCCCCCCEEEEEEEECCHHHHCCCCCCCCCEECCCCEE
MLFYTGNVRIGEQRDRHTTQCLATSTDGIHFTKQGPVVPELPPGVTPHCRDPKVIRHNDR
EEEEECCEEECCCCCCCHHEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCC
WLMLLGVQREDEIGRLAIYHSEDLKTWTFIALCGDELGDFGYMWECPDFFTLNQQDFIVI
EEEEEECCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCEEEECCCEEEECCCCEEEE
GPQGIRSPDKSHTAPHHNGIVKAQLETSGKALLSDFQPLDYGFDFYAPQSLETPDGRRIM
CCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEE
CAWMGLPDEIDHPSADNGWVHQLTTMRELSYDNGALIQKPIKELATLRHTPIVLSKDETS
EEECCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCC
FDLHSKAFELQVSMQWGSVLRLHQSESGYCEIRLDSASRRLYIDRSNTLIREGDTVREMS
CEECCEEEEEEEEECCCCEEEEECCCCCEEEEEECCCCCEEEEECCCCEEECCCCCEEEC
LAESDSVQLHIFSDTSSLEVFINEGEAVMSARVFTDKNSTQLSFDGDVQIQACWLLNKAS
CCCCCCEEEEEEECCCCEEEEEECCCEEEEEEEEECCCCCEEEECCCEEEEEEEEEECCC
APFIS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA