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The map label for this gene is mpl [H]

Identifier: 119944588

GI number: 119944588

Start: 1030900

End: 1032258

Strand: Direct

Name: mpl [H]

Synonym: Ping_0826

Alternate gene names: 119944588

Gene position: 1030900-1032258 (Clockwise)

Preceding gene: 119944587

Following gene: 119944589

Centisome position: 22.61

GC content: 40.03

Gene sequence:

>1359_bases
ATGCATATTCATATTCTTGGCATCTGTGGCACATTTATGGGGGGCCTTGCTATTCTCGCCAAATCCTTGGGTTATAAAGT
CACAGGATCAGATAAAAACGTTTATCCTCCGATGAGTACCCAATTAGAAGAGCAAGGGATTGAATTAATTCAAGGTTTTG
ATCCAGACCAATTAGATTCAAATCCTGATCTTGTGGTTATTGGGAATGCAATGAGTCGTGGGAATCCTTGTGTAGAAGCT
GTTCTGGATAAACAAATTCCTTATATTTCGGGTCCACAGTGGTTATTAGAGCATGTCTTAAAAGATCGTTGGGTATTAGC
TGTTGCCGGGACACACGGTAAAACGTCCACTGCAAGCATGTTGGCTTGGATTTTAGAATATGCCAATATGAAACCGGGTT
TCTTAATTGGTGGTGTACCGGAGAACTTTGGTCTGTCAGCCAGGTTAGGTGAAACCCCATTTTTTGTGATAGAGGCAGAT
GAGTATGACAGTGCTTTTTTTGATAAACGTTCCAAATTTGTTCACTATCACCCTCGAACGCTGGTTTTAAATAACTTAGA
GTTTGACCATGCAGATATTTTTGAAGATTTAAATGCCATTAAAAAACAATTTCATCATTTAGTTCGTACTGTTCCTGCAA
CAGGGCGTATTTTTATACCCGATCAGGATCAAGCTCTGATCGATGTCCAGCGCATGGGCTGTTGGAGTGAAGTTGAATAT
CTGGGGAAAAACTGGTCTTGCAAAAAAATCAAAGAAGATGCTTCAATTTTTGACGTCTATCTAAATCAGCAAAAACAGGG
GCAGGTGGCTTGGTCATTGATGGGTGACCATAATGTGAATAATGCACTTGCTGCCATAGCTGCTGCGCGCCACGTTGGTG
TTACGCCGGAAATTGCGATTGAAGCACTCGCTGAATTTAAAAATATCAAACGTCGAATGGAAGTTAAAGGCAAAGTTAAT
AGTATTACTGTTTATGATGATTTTGCTCATCATCCTACCGCCATTAAAACAACCTTAGCGGGGTTACGTGCAAAAGTAGG
CAATGAAAAAATTATTGCTGTTCTGGAGCCGCGTTCTAATACGATGAAATTAGGTGTCCATAAAGAGGCATTAGTCAATG
CATTAGCCGCCGCTGACAGTGTTTACTGTTTTGAAACAGAAGATATTAAATGGTCAATTGAAGAACTGTTTGCAGAGCAA
GGTAAAGCCGTTGATATATCCCATGATATTGATGAGTTATGCACTAAGTTAGTATCAATAGCAGAGCGAAACGCTCATAT
ACTCATTATGAGTAATGGTGGTTTTGGTAATATCCATAACAAGTTACTGGGGCAACTCGCCCTGAAAAAGAAGGTTTAA

Upstream 100 bases:

>100_bases
ATCAGGTAATTTAGGGTTAACAATTAAAGACACTTTCTTACCTTTCATGTTCAGGTGATTTTGGGTATAACAATTAAATA
CACTTTCCTATTGAGGTTTT

Downstream 100 bases:

>100_bases
TATGTCTAAAGATGTTTTTTTTAAAGATAGGATTACGTTAGCGGTAACAGGTGCATCGGGTAGCGGTTACTTCTTAAGGC
TATTACAGGCATTGGTTAAT

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MHIHILGICGTFMGGLAILAKSLGYKVTGSDKNVYPPMSTQLEEQGIELIQGFDPDQLDSNPDLVVIGNAMSRGNPCVEA
VLDKQIPYISGPQWLLEHVLKDRWVLAVAGTHGKTSTASMLAWILEYANMKPGFLIGGVPENFGLSARLGETPFFVIEAD
EYDSAFFDKRSKFVHYHPRTLVLNNLEFDHADIFEDLNAIKKQFHHLVRTVPATGRIFIPDQDQALIDVQRMGCWSEVEY
LGKNWSCKKIKEDASIFDVYLNQQKQGQVAWSLMGDHNVNNALAAIAAARHVGVTPEIAIEALAEFKNIKRRMEVKGKVN
SITVYDDFAHHPTAIKTTLAGLRAKVGNEKIIAVLEPRSNTMKLGVHKEALVNALAAADSVYCFETEDIKWSIEELFAEQ
GKAVDISHDIDELCTKLVSIAERNAHILIMSNGGFGNIHNKLLGQLALKKKV

Sequences:

>Translated_452_residues
MHIHILGICGTFMGGLAILAKSLGYKVTGSDKNVYPPMSTQLEEQGIELIQGFDPDQLDSNPDLVVIGNAMSRGNPCVEA
VLDKQIPYISGPQWLLEHVLKDRWVLAVAGTHGKTSTASMLAWILEYANMKPGFLIGGVPENFGLSARLGETPFFVIEAD
EYDSAFFDKRSKFVHYHPRTLVLNNLEFDHADIFEDLNAIKKQFHHLVRTVPATGRIFIPDQDQALIDVQRMGCWSEVEY
LGKNWSCKKIKEDASIFDVYLNQQKQGQVAWSLMGDHNVNNALAAIAAARHVGVTPEIAIEALAEFKNIKRRMEVKGKVN
SITVYDDFAHHPTAIKTTLAGLRAKVGNEKIIAVLEPRSNTMKLGVHKEALVNALAAADSVYCFETEDIKWSIEELFAEQ
GKAVDISHDIDELCTKLVSIAERNAHILIMSNGGFGNIHNKLLGQLALKKKV
>Mature_452_residues
MHIHILGICGTFMGGLAILAKSLGYKVTGSDKNVYPPMSTQLEEQGIELIQGFDPDQLDSNPDLVVIGNAMSRGNPCVEA
VLDKQIPYISGPQWLLEHVLKDRWVLAVAGTHGKTSTASMLAWILEYANMKPGFLIGGVPENFGLSARLGETPFFVIEAD
EYDSAFFDKRSKFVHYHPRTLVLNNLEFDHADIFEDLNAIKKQFHHLVRTVPATGRIFIPDQDQALIDVQRMGCWSEVEY
LGKNWSCKKIKEDASIFDVYLNQQKQGQVAWSLMGDHNVNNALAAIAAARHVGVTPEIAIEALAEFKNIKRRMEVKGKVN
SITVYDDFAHHPTAIKTTLAGLRAKVGNEKIIAVLEPRSNTMKLGVHKEALVNALAAADSVYCFETEDIKWSIEELFAEQ
GKAVDISHDIDELCTKLVSIAERNAHILIMSNGGFGNIHNKLLGQLALKKKV

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=454, Percent_Identity=63.4361233480176, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1786279, Length=408, Percent_Identity=28.1862745098039, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50098; Mature: 50098

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAILAKSLGYKVTGSDKNVYPPMSTQLEEQGIELIQGFDPDQLDS
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
NPDLVVIGNAMSRGNPCVEAVLDKQIPYISGPQWLLEHVLKDRWVLAVAGTHGKTSTASM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEYANMKPGFLIGGVPENFGLSARLGETPFFVIEADEYDSAFFDKRSKFVHYHPRT
HHHHHHHHCCCCCEEECCCCCCCCCCEECCCCCEEEEECCCCCHHHHHCCCCEEEECCCE
LVLNNLEFDHADIFEDLNAIKKQFHHLVRTVPATGRIFIPDQDQALIDVQRMGCWSEVEY
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCHHHHHH
LGKNWSCKKIKEDASIFDVYLNQQKQGQVAWSLMGDHNVNNALAAIAAARHVGVTPEIAI
HCCCCCHHHHHCCHHHEEHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHH
EALAEFKNIKRRMEVKGKVNSITVYDDFAHHPTAIKTTLAGLRAKVGNEKIIAVLEPRSN
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
TMKLGVHKEALVNALAAADSVYCFETEDIKWSIEELFAEQGKAVDISHDIDELCTKLVSI
EEEECCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHHHH
AERNAHILIMSNGGFGNIHNKLLGQLALKKKV
HCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAILAKSLGYKVTGSDKNVYPPMSTQLEEQGIELIQGFDPDQLDS
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
NPDLVVIGNAMSRGNPCVEAVLDKQIPYISGPQWLLEHVLKDRWVLAVAGTHGKTSTASM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEYANMKPGFLIGGVPENFGLSARLGETPFFVIEADEYDSAFFDKRSKFVHYHPRT
HHHHHHHHCCCCCEEECCCCCCCCCCEECCCCCEEEEECCCCCHHHHHCCCCEEEECCCE
LVLNNLEFDHADIFEDLNAIKKQFHHLVRTVPATGRIFIPDQDQALIDVQRMGCWSEVEY
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCHHHHHH
LGKNWSCKKIKEDASIFDVYLNQQKQGQVAWSLMGDHNVNNALAAIAAARHVGVTPEIAI
HCCCCCHHHHHCCHHHEEHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHH
EALAEFKNIKRRMEVKGKVNSITVYDDFAHHPTAIKTTLAGLRAKVGNEKIIAVLEPRSN
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
TMKLGVHKEALVNALAAADSVYCFETEDIKWSIEELFAEQGKAVDISHDIDELCTKLVSI
EEEECCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHHHH
AERNAHILIMSNGGFGNIHNKLLGQLALKKKV
HCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]