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The map label for this gene is pnp

Identifier: 119944587

GI number: 119944587

Start: 1028524

End: 1030620

Strand: Direct

Name: pnp

Synonym: Ping_0825

Alternate gene names: 119944587

Gene position: 1028524-1030620 (Clockwise)

Preceding gene: 119944586

Following gene: 119944588

Centisome position: 22.56

GC content: 43.82

Gene sequence:

>2097_bases
ATGACTCCTATTGTTAAAAGTTTTCAGTATGGCGAACATACTGTCACTCTAGAAACGGGCGTAATGGCTCGTCAGGCAAC
TGCCGCTGTAATGTGTAGTATGGATGATACCTGTGTATTCGTTTCTGTTGTTGGTAAAAATGCTGACAACCAAGAGCGTG
ATTTCTTTCCGCTGACGGTAAACTACCAGGAAAAAACCTATGCTGCGGGTAAAATCCCAGGTGGTTTCTTCAAACGTGAA
GGCCGTCCAAGCGAAGACGAAACACTGATTGCACGCCTTATTGACCGTCCAGTTCGTCCTCTATTCCCAGCCGGTTTCAA
AAACGAAGTACAAGTGATTGCAACTGTTGTCTCTATCAACCCTGAAGTTAACCCGGATGTCGTTGCAATGATTGCAACAT
CTGCGGCGCTTTCTATCTCGGGTATGCCTTTCAAGGGGCCTATCGGTTGTGCACGTGTTGGTTATAAAGAAGGTGAATAC
CTGCTTAACCCAACCAAAACTGAATTAGAAACAAGTGATCTTAACCTTGTTGTATCTGGTACAGATAACGCTGTCTTAAT
GGTTGAATCTGAAGCCGGTATTCTAAGCGAAGACGTTATGCTTGGTGCGGTTGTATTTGGTCACGATCAGCAACAAATTG
TTGTTGAATCAATCAAAGAGTTCGCTGCAGAGGTTGCTCGTCCAGCATGGAACTGGGCTGCCCCTGCACGTAACGAAGCA
TTAGATGCGGCTATTGCAACTGAAGCACAAGCGCGTTTTGAACAAGCTTACCAGATCAGTGATAAAGGCGATCGTTACGA
TGCGATTAAAGCTATCACCTCTGAAGTTAAAGAAAAATTATTAGCATCTGATGAAACATTAGAAGCTAAGCAAATCGGTG
AATACTTACACGATCTAGAAAAAACAGTGGTACGTACGCGCATTATTAAAGGTGAGCCGCGTATCGATGGTCGCGATCCT
GAAAGTATTCGTGCATTAGATGTCCGTACTGGCGTGTTACCTCGTACTCACGGTAGTGCATTGTTTACCCGTGGTGAAAC
TCAAGCATTAGTGACGGCGACCCTTGGTACTCAGCGTGATGCGCAAATCATTGACAGTATAATGGGTGAGAAGAAAGATA
ACTTCTTATTACATTACAACTTCCCTCCTTATTGTGTAGGCGAAACTGGCTTTGTTGGCTCTCCTAAACGTCGTGAGATT
GGCCATGGCCGTCTTGCTAAACGTGGTGTTTTGGCTGTTATGCCTTCGCTTGAAGAATTCCCATACACAGTACGTGTTGT
GTCTGAAATCACTGAATCAAACGGTTCATCTTCAATGGCTTCTGTTTGTGGTGCATCGCTAGCATTAATGGATGCGGGTG
TTCCTCTTAAAGCTTCTGTTGCGGGAATCGCGATGGGTCTTGTAAAAGAAGGCGAAGAGTTTGTTGTGCTTTCTGATATT
CTAGGTGATGAAGATCATCTTGGTGATATGGACTTTAAAGTTGCCGGTACTACCGAAGGTATTACTGCACTACAGATGGA
TATCAAAATTGAAGGGATCACGCGTGAAATCATGCAGATTGCTTTAAATCAGGCAAAAGCTGCACGTTTGCACATTTTGA
CCGTAATGGACCAAGCACTTCCACAAGGTCGCGCTGATATTTCTCAATTCGCTCCACGTATTCATACTATCAAAATCAAC
ACTGATAAAATCCGTGATGTTATTGGTAAAGGCGGCGCTGTTATCCGCTCTCTATGTGAAGAAACCGGTACTACAATTGA
AATCGAAGATGACGGTACTGTTAAAATTGCAGCGACCTCTGGTGAGCAAGCTGATGACGCAATTAACCGTATTAAAGCAC
TCACAGCTGAAGTTGAAGTGGGTACTATCTACACAGGTAAAGTGGTACGTTTAGCTGATTTCGGTGCCTTTGTTAATATC
CTTCCCGGTAAAGATGGTCTAGTACATATCTCGCAAATCTGTGAAGAGCGTGTACAGAAAGTGTCTGATCACCTTAAAGA
AGGTCAGGAAGTGAAAGTTAAAGTATTAGAAGTTGATCGCCAGGGCCGTGTGCGCTTAAGCATCAAAGAAGCTGCTGAAA
AAACAACAGCTGAATAA

Upstream 100 bases:

>100_bases
TTTGTTTCTGCAGATTAAAAAATGTTTAAAACGTAAAGGTTGACGTATACTAGCGGCGCGATCAAAAGCACAAGAATATA
AATTTATTAAAGGAAAAAAA

Downstream 100 bases:

>100_bases
TACCTTGATTGGTAAAAAATAAAGTAATGACTGCGGTCATTGACTCAAAAAAAGGAAGCTGCGGCTTCCTTTTTTTATAA
GCGTCCAAAACATCTGCTTT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MTPIVKSFQYGEHTVTLETGVMARQATAAVMCSMDDTCVFVSVVGKNADNQERDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPVRPLFPAGFKNEVQVIATVVSINPEVNPDVVAMIATSAALSISGMPFKGPIGCARVGYKEGEY
LLNPTKTELETSDLNLVVSGTDNAVLMVESEAGILSEDVMLGAVVFGHDQQQIVVESIKEFAAEVARPAWNWAAPARNEA
LDAAIATEAQARFEQAYQISDKGDRYDAIKAITSEVKEKLLASDETLEAKQIGEYLHDLEKTVVRTRIIKGEPRIDGRDP
ESIRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDSIMGEKKDNFLLHYNFPPYCVGETGFVGSPKRREI
GHGRLAKRGVLAVMPSLEEFPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPLKASVAGIAMGLVKEGEEFVVLSDI
LGDEDHLGDMDFKVAGTTEGITALQMDIKIEGITREIMQIALNQAKAARLHILTVMDQALPQGRADISQFAPRIHTIKIN
TDKIRDVIGKGGAVIRSLCEETGTTIEIEDDGTVKIAATSGEQADDAINRIKALTAEVEVGTIYTGKVVRLADFGAFVNI
LPGKDGLVHISQICEERVQKVSDHLKEGQEVKVKVLEVDRQGRVRLSIKEAAEKTTAE

Sequences:

>Translated_698_residues
MTPIVKSFQYGEHTVTLETGVMARQATAAVMCSMDDTCVFVSVVGKNADNQERDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPVRPLFPAGFKNEVQVIATVVSINPEVNPDVVAMIATSAALSISGMPFKGPIGCARVGYKEGEY
LLNPTKTELETSDLNLVVSGTDNAVLMVESEAGILSEDVMLGAVVFGHDQQQIVVESIKEFAAEVARPAWNWAAPARNEA
LDAAIATEAQARFEQAYQISDKGDRYDAIKAITSEVKEKLLASDETLEAKQIGEYLHDLEKTVVRTRIIKGEPRIDGRDP
ESIRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDSIMGEKKDNFLLHYNFPPYCVGETGFVGSPKRREI
GHGRLAKRGVLAVMPSLEEFPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPLKASVAGIAMGLVKEGEEFVVLSDI
LGDEDHLGDMDFKVAGTTEGITALQMDIKIEGITREIMQIALNQAKAARLHILTVMDQALPQGRADISQFAPRIHTIKIN
TDKIRDVIGKGGAVIRSLCEETGTTIEIEDDGTVKIAATSGEQADDAINRIKALTAEVEVGTIYTGKVVRLADFGAFVNI
LPGKDGLVHISQICEERVQKVSDHLKEGQEVKVKVLEVDRQGRVRLSIKEAAEKTTAE
>Mature_697_residues
TPIVKSFQYGEHTVTLETGVMARQATAAVMCSMDDTCVFVSVVGKNADNQERDFFPLTVNYQEKTYAAGKIPGGFFKREG
RPSEDETLIARLIDRPVRPLFPAGFKNEVQVIATVVSINPEVNPDVVAMIATSAALSISGMPFKGPIGCARVGYKEGEYL
LNPTKTELETSDLNLVVSGTDNAVLMVESEAGILSEDVMLGAVVFGHDQQQIVVESIKEFAAEVARPAWNWAAPARNEAL
DAAIATEAQARFEQAYQISDKGDRYDAIKAITSEVKEKLLASDETLEAKQIGEYLHDLEKTVVRTRIIKGEPRIDGRDPE
SIRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDSIMGEKKDNFLLHYNFPPYCVGETGFVGSPKRREIG
HGRLAKRGVLAVMPSLEEFPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPLKASVAGIAMGLVKEGEEFVVLSDIL
GDEDHLGDMDFKVAGTTEGITALQMDIKIEGITREIMQIALNQAKAARLHILTVMDQALPQGRADISQFAPRIHTIKINT
DKIRDVIGKGGAVIRSLCEETGTTIEIEDDGTVKIAATSGEQADDAINRIKALTAEVEVGTIYTGKVVRLADFGAFVNIL
PGKDGLVHISQICEERVQKVSDHLKEGQEVKVKVLEVDRQGRVRLSIKEAAEKTTAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=36.7897727272727, Blast_Score=425, Evalue=1e-119,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=73.6690647482014, Blast_Score=1068, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=670, Percent_Identity=33.4328358208955, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17535281, Length=80, Percent_Identity=46.25, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281362905, Length=708, Percent_Identity=38.4180790960452, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=708, Percent_Identity=38.4180790960452, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=708, Percent_Identity=38.4180790960452, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=654, Percent_Identity=38.3792048929664, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PSYIN (A1ST53)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942267.1
- ProteinModelPortal:   A1ST53
- STRING:   A1ST53
- GeneID:   4625467
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0825
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   A1ST53
- ProtClustDB:   PRK11824
- BioCyc:   PING357804:PING_0825-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75644; Mature: 75513

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPIVKSFQYGEHTVTLETGVMARQATAAVMCSMDDTCVFVSVVGKNADNQERDFFPLTV
CCCCCCCCCCCCEEEEEECCCHHHHHHEEEEECCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPVRPLFPAGFKNEVQVIATVVSIN
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECC
PEVNPDVVAMIATSAALSISGMPFKGPIGCARVGYKEGEYLLNPTKTELETSDLNLVVSG
CCCCCCCEEEEEHHHHEEECCCCCCCCCCHHHCCCCCCCEEECCCCCCEECCCCEEEEEC
TDNAVLMVESEAGILSEDVMLGAVVFGHDQQQIVVESIKEFAAEVARPAWNWAAPARNEA
CCCEEEEEECCCCCCCCHHEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
LDAAIATEAQARFEQAYQISDKGDRYDAIKAITSEVKEKLLASDETLEAKQIGEYLHDLE
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KTVVRTRIIKGEPRIDGRDPESIRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRD
HHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCEEEEEECCCCCH
AQIIDSIMGEKKDNFLLHYNFPPYCVGETGFVGSPKRREIGHGRLAKRGVLAVMPSLEEF
HHHHHHHHCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHCCCEEECCCHHHC
PYTVRVVSEITESNGSSSMASVCGASLALMDAGVPLKASVAGIAMGLVKEGEEFVVLSDI
CCHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEHH
LGDEDHLGDMDFKVAGTTEGITALQMDIKIEGITREIMQIALNQAKAARLHILTVMDQAL
CCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PQGRADISQFAPRIHTIKINTDKIRDVIGKGGAVIRSLCEETGTTIEIEDDGTVKIAATS
CCCCCCHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEEEECC
GEQADDAINRIKALTAEVEVGTIYTGKVVRLADFGAFVNILPGKDGLVHISQICEERVQK
CCCHHHHHHHHHHHHEEEEEEEEECCCEEEEEECCCEEEEECCCCCEEHHHHHHHHHHHH
VSDHLKEGQEVKVKVLEVDRQGRVRLSIKEAAEKTTAE
HHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHHHCCCC
>Mature Secondary Structure 
TPIVKSFQYGEHTVTLETGVMARQATAAVMCSMDDTCVFVSVVGKNADNQERDFFPLTV
CCCCCCCCCCCEEEEEECCCHHHHHHEEEEECCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPVRPLFPAGFKNEVQVIATVVSIN
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECC
PEVNPDVVAMIATSAALSISGMPFKGPIGCARVGYKEGEYLLNPTKTELETSDLNLVVSG
CCCCCCCEEEEEHHHHEEECCCCCCCCCCHHHCCCCCCCEEECCCCCCEECCCCEEEEEC
TDNAVLMVESEAGILSEDVMLGAVVFGHDQQQIVVESIKEFAAEVARPAWNWAAPARNEA
CCCEEEEEECCCCCCCCHHEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
LDAAIATEAQARFEQAYQISDKGDRYDAIKAITSEVKEKLLASDETLEAKQIGEYLHDLE
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KTVVRTRIIKGEPRIDGRDPESIRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRD
HHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCEEEEEECCCCCH
AQIIDSIMGEKKDNFLLHYNFPPYCVGETGFVGSPKRREIGHGRLAKRGVLAVMPSLEEF
HHHHHHHHCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHCCCEEECCCHHHC
PYTVRVVSEITESNGSSSMASVCGASLALMDAGVPLKASVAGIAMGLVKEGEEFVVLSDI
CCHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEHH
LGDEDHLGDMDFKVAGTTEGITALQMDIKIEGITREIMQIALNQAKAARLHILTVMDQAL
CCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PQGRADISQFAPRIHTIKINTDKIRDVIGKGGAVIRSLCEETGTTIEIEDDGTVKIAATS
CCCCCCHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEEEECC
GEQADDAINRIKALTAEVEVGTIYTGKVVRLADFGAFVNILPGKDGLVHISQICEERVQK
CCCHHHHHHHHHHHHEEEEEEEEECCCEEEEEECCCEEEEECCCCCEEHHHHHHHHHHHH
VSDHLKEGQEVKVKVLEVDRQGRVRLSIKEAAEKTTAE
HHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA