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The map label for this gene is nth [H]

Identifier: 119944503

GI number: 119944503

Start: 925923

End: 926558

Strand: Reverse

Name: nth [H]

Synonym: Ping_0732

Alternate gene names: 119944503

Gene position: 926558-925923 (Counterclockwise)

Preceding gene: 119944504

Following gene: 119944485

Centisome position: 20.32

GC content: 41.98

Gene sequence:

>636_bases
ATGAATGATATTAAACGGCGCGAAATATTAATGCGTCTGCGAGCTGAAAATCCAACACCACAAACTGAACTTAATTACAG
CTCTCCCTTTGAATTGTTAATTTCAGTTATCCTGTCTGCACAAGCAACTGACGTCAGTGTTAACAAAGCAACCGCTCTGC
TTTATCCCGTTGCCAATACACCAGAGACAATCGCAGCATTAGGCGTCGAGGGCTTAAAGCGCTACATTAAAACCATTGGT
TTATTTAACAGTAAAGCCGCCAACGTGATCAAAACCTGTAACCAGTTAATCACATACCATAATAGTGAAGTGCCTGAAAA
TAGAGAAGCGTTAGAGGCCTTGCCCGGTGTGGGACGTAAAACAGCTAATGTGGTGTTAAATACGGCATTTGGCTGGCCGA
CAATAGCAGTAGATACGCATATTTTCCGCGTCTCTAATCGCAGCAAATTAGCCATGGGCAAAAGTGTGGAGGAAGTAGAA
AAAAAATTGCTAAAAGTGATACCCACTGAATTTAAACTTGATGTGCACCATTGGCTTATTTTACACGGCCGTTATACCTG
TGTTGCCCGCAAACCCCACTGTGGTTCCTGCCTGATTGAAGATTTATGCGAGTTTAAAGAAAAAACAGAAGATTAA

Upstream 100 bases:

>100_bases
ATATTAAACGTCGCAAAATATTAATGCATTTATCGACTGAAAAGTAATGAGCGATATTAAACGTCGCAAAATATTAATGC
ATTTATGGGACTGAAAAATA

Downstream 100 bases:

>100_bases
GTTGTCTCATTAGCTGATCTATTTTAATCAGTGCGCAGATGCGAGCCTGAAGGCTGACAGCTGACGGCTAACAACTGACA
GCTAACAGCTGACGGCTAAC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNDIKRREILMRLRAENPTPQTELNYSSPFELLISVILSAQATDVSVNKATALLYPVANTPETIAALGVEGLKRYIKTIG
LFNSKAANVIKTCNQLITYHNSEVPENREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVSNRSKLAMGKSVEEVE
KKLLKVIPTEFKLDVHHWLILHGRYTCVARKPHCGSCLIEDLCEFKEKTED

Sequences:

>Translated_211_residues
MNDIKRREILMRLRAENPTPQTELNYSSPFELLISVILSAQATDVSVNKATALLYPVANTPETIAALGVEGLKRYIKTIG
LFNSKAANVIKTCNQLITYHNSEVPENREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVSNRSKLAMGKSVEEVE
KKLLKVIPTEFKLDVHHWLILHGRYTCVARKPHCGSCLIEDLCEFKEKTED
>Mature_211_residues
MNDIKRREILMRLRAENPTPQTELNYSSPFELLISVILSAQATDVSVNKATALLYPVANTPETIAALGVEGLKRYIKTIG
LFNSKAANVIKTCNQLITYHNSEVPENREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVSNRSKLAMGKSVEEVE
KKLLKVIPTEFKLDVHHWLILHGRYTCVARKPHCGSCLIEDLCEFKEKTED

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=88, Evalue=6e-18,
Organism=Escherichia coli, GI1787920, Length=210, Percent_Identity=77.1428571428572, Blast_Score=342, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=31.4285714285714, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=26.2032085561497, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=31.5508021390374, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23614; Mature: 23614

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIKRREILMRLRAENPTPQTELNYSSPFELLISVILSAQATDVSVNKATALLYPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
PETIAALGVEGLKRYIKTIGLFNSKAANVIKTCNQLITYHNSEVPENREALEALPGVGRK
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRVSNRSKLAMGKSVEEVEKKLLKVIPTEFKLDVHHWLI
HHHEEEECCCCCCEEEECCEEEEECCCCHHHHCCCHHHHHHHHHHHCCCHHEEEHHHEEE
LHGRYTCVARKPHCGSCLIEDLCEFKEKTED
EECCEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDIKRREILMRLRAENPTPQTELNYSSPFELLISVILSAQATDVSVNKATALLYPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
PETIAALGVEGLKRYIKTIGLFNSKAANVIKTCNQLITYHNSEVPENREALEALPGVGRK
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRVSNRSKLAMGKSVEEVEKKLLKVIPTEFKLDVHHWLI
HHHEEEECCCCCCEEEECCEEEEECCCCHHHHCCCHHHHHHHHHHHCCCHHEEEHHHEEE
LHGRYTCVARKPHCGSCLIEDLCEFKEKTED
EECCEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]