Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 119944490

GI number: 119944490

Start: 912398

End: 913402

Strand: Direct

Name: ruvB

Synonym: Ping_0719

Alternate gene names: 119944490

Gene position: 912398-913402 (Clockwise)

Preceding gene: 119944489

Following gene: 119944491

Centisome position: 20.01

GC content: 43.58

Gene sequence:

>1005_bases
ATGATAGATGAAGATCGTCTGATTTCAGGCACACAAAAGATTGATGAAAATTTTATTGATCGGGCTATCCGTCCAAAATT
TTTAGCCGATTACGAAGGCCAGCCGCAGGTTAATAAGCAGATGGAAATTTTTATTGAGGCGGCACGTCAACGTAATGAGG
CATTAGATCACCTGCTTATTTTTGGTCCGCCAGGATTGGGTAAAACAACCTTATCTCATATTGTTGCCAACGAACTGGAG
GTGAATATTAAAACCACCTCGGGACCTATTCTTGAAAAGGCCGGTGATTTAGCGGCGTTGTTAACCAATCTTGAAGAAAA
TGATGTGTTATTTATTGATGAGATACACAGATTAAGCCCGGTTGTGGAGGAAATATTATATCCTGCGATGGAAGATTACC
AACTGGATATTATGATTGGTGAAGGTCCCGCTGCGCGCTCTATTAAACTTGATCTCCCCGCATTTACCTTAATCGGTGCA
ACAACGCGAGCAGGATCCTTAACTTCTCCTTTGCGAGACCGTTTTGGTATTGTGCAGCGCCTTGAATATTACGACCTTAA
ATCACTCACGCGGATAGTGCTGCGCAGTGCCGGACATTTTAAGCTTAATATGAGTGAAGCAGGTGCCGTTGAGATTGCCC
GGCGCTCACGCGGTACACCGCGTATCGCTAATCGTTTATTACGTCGGGTGCGGGATTATGCTCAGGTTAAAAAAGCCAAT
GTTATTGATGACGATGTCGCAAAATTAGCCCTGGATATGCTCGAAGTGGATAATGAAGGCTTTGATTATATGGATAGAAA
ATTGTTGATGGCGATTTTAGATACTTTTATGGGCGGACCGGTCGGCTTAGATAATTTAGCCGCTGCCATTGGTGAAGAGA
AAGAAACCATTGAAGATGTCCTAGAGCCCTACCTTATTCAGCAGGGGTTTTTACAGCGTACACCTCGCGGACGCATTGCC
ACTAATAGAACCTATCTACACTTTGGGTTTGATAAACCTCAATAA

Upstream 100 bases:

>100_bases
CCAGCAAGTTTATGTGGAAGGCATGGATTGTGAGGCTTTGATCCGCAGTTCATTAAAAAGTATGCTCTAAACGATAATCA
TAAGAAAGGTCGGGTAAAAA

Downstream 100 bases:

>100_bases
CGTAAGATGAAAAAAATATAAGACTCTTTGAGGGCCCAGGTGTCTTCAAAGAGTAAGGAAATCAATAACAATATTAATTA
TTAATGTCTGTTGATTTTGT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MIDEDRLISGTQKIDENFIDRAIRPKFLADYEGQPQVNKQMEIFIEAARQRNEALDHLLIFGPPGLGKTTLSHIVANELE
VNIKTTSGPILEKAGDLAALLTNLEENDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPAFTLIGA
TTRAGSLTSPLRDRFGIVQRLEYYDLKSLTRIVLRSAGHFKLNMSEAGAVEIARRSRGTPRIANRLLRRVRDYAQVKKAN
VIDDDVAKLALDMLEVDNEGFDYMDRKLLMAILDTFMGGPVGLDNLAAAIGEEKETIEDVLEPYLIQQGFLQRTPRGRIA
TNRTYLHFGFDKPQ

Sequences:

>Translated_334_residues
MIDEDRLISGTQKIDENFIDRAIRPKFLADYEGQPQVNKQMEIFIEAARQRNEALDHLLIFGPPGLGKTTLSHIVANELE
VNIKTTSGPILEKAGDLAALLTNLEENDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPAFTLIGA
TTRAGSLTSPLRDRFGIVQRLEYYDLKSLTRIVLRSAGHFKLNMSEAGAVEIARRSRGTPRIANRLLRRVRDYAQVKKAN
VIDDDVAKLALDMLEVDNEGFDYMDRKLLMAILDTFMGGPVGLDNLAAAIGEEKETIEDVLEPYLIQQGFLQRTPRGRIA
TNRTYLHFGFDKPQ
>Mature_334_residues
MIDEDRLISGTQKIDENFIDRAIRPKFLADYEGQPQVNKQMEIFIEAARQRNEALDHLLIFGPPGLGKTTLSHIVANELE
VNIKTTSGPILEKAGDLAALLTNLEENDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPAFTLIGA
TTRAGSLTSPLRDRFGIVQRLEYYDLKSLTRIVLRSAGHFKLNMSEAGAVEIARRSRGTPRIANRLLRRVRDYAQVKKAN
VIDDDVAKLALDMLEVDNEGFDYMDRKLLMAILDTFMGGPVGLDNLAAAIGEEKETIEDVLEPYLIQQGFLQRTPRGRIA
TNRTYLHFGFDKPQ

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=334, Percent_Identity=75.748502994012, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_PSYIN (A1SSV6)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942170.1
- ProteinModelPortal:   A1SSV6
- SMR:   A1SSV6
- STRING:   A1SSV6
- GeneID:   4625066
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0719
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   A1SSV6
- ProtClustDB:   PRK00080
- BioCyc:   PING357804:PING_0719-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37522; Mature: 37522

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDEDRLISGTQKIDENFIDRAIRPKFLADYEGQPQVNKQMEIFIEAARQRNEALDHLLI
CCCCCHHHCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEE
FGPPGLGKTTLSHIVANELEVNIKTTSGPILEKAGDLAALLTNLEENDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
VVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPAFTLIGATTRAGSLTSPLRDRFGIVQR
HHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHH
LEYYDLKSLTRIVLRSAGHFKLNMSEAGAVEIARRSRGTPRIANRLLRRVRDYAQVKKAN
HHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
VIDDDVAKLALDMLEVDNEGFDYMDRKLLMAILDTFMGGPVGLDNLAAAIGEEKETIEDV
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHH
LEPYLIQQGFLQRTPRGRIATNRTYLHFGFDKPQ
HHHHHHHHHHHHCCCCCCEECCCEEEEECCCCCC
>Mature Secondary Structure
MIDEDRLISGTQKIDENFIDRAIRPKFLADYEGQPQVNKQMEIFIEAARQRNEALDHLLI
CCCCCHHHCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEE
FGPPGLGKTTLSHIVANELEVNIKTTSGPILEKAGDLAALLTNLEENDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
VVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPAFTLIGATTRAGSLTSPLRDRFGIVQR
HHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHH
LEYYDLKSLTRIVLRSAGHFKLNMSEAGAVEIARRSRGTPRIANRLLRRVRDYAQVKKAN
HHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
VIDDDVAKLALDMLEVDNEGFDYMDRKLLMAILDTFMGGPVGLDNLAAAIGEEKETIEDV
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHH
LEPYLIQQGFLQRTPRGRIATNRTYLHFGFDKPQ
HHHHHHHHHHHHCCCCCCEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA