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The map label for this gene is thiC [H]

Identifier: 119944141

GI number: 119944141

Start: 462610

End: 464580

Strand: Direct

Name: thiC [H]

Synonym: Ping_0360

Alternate gene names: 119944141

Gene position: 462610-464580 (Clockwise)

Preceding gene: 119944140

Following gene: 119944142

Centisome position: 10.15

GC content: 43.63

Gene sequence:

>1971_bases
ATGTCTAATCAAGTAAACTCGAATTTAAAAACCAGCAGCCGTAAAGGTTTATCCCGCAAAGCCTCCCGCCGAGCAGCGCA
GGACTATATTCAAAATCTCAATAACGAATCCTACCCCAATTCAGAAAAAATTTTTATTAAGGGTGAACTTCATCCGATTA
AAGTGGCAATGCGACAAATTCATCAGGCCGATACTTTGCTGGCAGGCAGTGGAGAACAGCCCGTTTTTGAAAAAAATGAA
GCTATTCCCGTTTATGATACTTCCGGCCCTTATACGGATCCTAAAACGAAAATAGATGTGCATGCCGGCTTACCTAAATT
ACGTCAATCCTGGATTGCTGCCCGTGATGATGTTGAAACACTGGAAGGTGTCACCTCAAATTTTGCCAAGGCACGTTTGG
CTAATGACAGTTTAGATGATTTGCGCTTTGCAAATCGGCCTAAACCCTTACGCGCTAAAAAAGGACAATGTGTCACCCAA
ATGCACTATGCACGCCGAGGTATTATTACCCCTGAAATGGAATACATTGCTATTCGTGAAAACATGGGCCGTGACCGCGT
AAAATGTGAAGCGCTGCATTTTCAGCATGCGGGTCATAGTTTTGGTGCCAATCTGCAGGAAAAAATAACCCCGGAGTTTG
TGCGTCAGGAAGTTGCTCGCGGACGTGCCATTATCCCGTCCAATATTAATCATCCGGAACTTGAGCCAATGATTATCGGC
CGTAATTTTCTGGTAAAAGTAAATTCTAATATTGGTAACTCTTCGGTAAGCTCTTCAATTGAAGAGGAGGTTGAAAAGCT
GGTTTGGTCAACGCTGTGGGGTGGTGATACGGTAATGGACTTGTCGACCGGGCGTAATATTCATGAAACCCGTGAATGGA
TCCTGCGTAACTCTCCCGTGCCAATCGGTACAGTACCTATCTATCAGGCACTGGAAAAAGTAAAGGGTGTGGCTGAGGAT
CTCAGTTGGGACGTTTTCCGTGACACCTTAATTGAACAGGCCGAGCAAGGGGTGGATTATTTTACTATTCATGCCGGTGT
ATTATTACGTTTTGTGCCGATGACAGCAAAACGTGTTACGGGTATTGTGTCTCGCGGCGGTTCAATCTTTGCAAAGTGGT
GCTTATCGCATCATAAAGAAAACTTTGCTTATGAATATTTTCGCGAAATTTGTGAAATCTGTCAGCTATATGATGTGTCA
TTATCTTTAGGTGATGGTCTGCGGCCCGGATCAGTTGCAGATGCTAATGATCAGGCACAATTTGCAGAATTGGAAGTGTT
AGGCGAACTGACTAAAGTTGCCTGGGAATACGATGTACAGGTGATTATTGAAGGTCCGGGTCATGTGCCGATGCAGATGA
TTAAAGAGAATATGGATAAACAGCTGGACGATTGTTTTGAAGCGCCATTTTATACCTTAGGGCCGCTAACCACGGATATT
GCACCGGGATATGATCATATTACGTCGGGCATAGGTGCCGCGCAAATTGGTTGGTACGGTTGTGCAATGCTTTGTTATGT
CACCCCAAAAGAGCATTTAGGTCTGCCAAATAAAGATGATGTAAAAGTCGGTTTAATTACTTACAAAATTGCAGCGCATG
CCGCGGATTTAGCAAAAGGGCATCCAGGTGCGCAAATTCGTGATAATGCCTTGTCAAAATCACGGTTTGAATTTCGCTGG
GAAGATCAGTTTAACCTCGGTTTAGATCCAGTCACCGCACGAGAATATCATGATGAAACCCTGCCGCAGGAATCCGGAAA
AGTCGCGCATTTCTGCTCAATGTGCGGGCCAAAATTCTGTTCAATGAAAATTACTCAGGATGTGCGTGATTTCACTGCAA
AATTAGATTCAGGTGAGATCAGTATTAAGATTTTAGATGAAACTATTACTATGACAGCAGCAGAAGGCATGGCACTTAAA
GCCAAAGAATTTAACGACTCTGGTGCTGAAATTTATCAGCCAGTTTTATAA

Upstream 100 bases:

>100_bases
AGGAAACAAGCATAAAGAAAATGAATCTCCTTTCTTTACTCTTTCCACTGAATTTCGACAAGCACTCTCACTACCCAATT
TAATTAAGGTAAAGAATGCT

Downstream 100 bases:

>100_bases
AAAAGCTGTCGGCTGTCGGCTGTTAGCTGTCAGCAGATAGCTGCTCTTGTTAAGGATAAATATGAATAAACTTGTTTGGA
GTATTGGTGCTTCTGATTGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MSNQVNSNLKTSSRKGLSRKASRRAAQDYIQNLNNESYPNSEKIFIKGELHPIKVAMRQIHQADTLLAGSGEQPVFEKNE
AIPVYDTSGPYTDPKTKIDVHAGLPKLRQSWIAARDDVETLEGVTSNFAKARLANDSLDDLRFANRPKPLRAKKGQCVTQ
MHYARRGIITPEMEYIAIRENMGRDRVKCEALHFQHAGHSFGANLQEKITPEFVRQEVARGRAIIPSNINHPELEPMIIG
RNFLVKVNSNIGNSSVSSSIEEEVEKLVWSTLWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPIYQALEKVKGVAED
LSWDVFRDTLIEQAEQGVDYFTIHAGVLLRFVPMTAKRVTGIVSRGGSIFAKWCLSHHKENFAYEYFREICEICQLYDVS
LSLGDGLRPGSVADANDQAQFAELEVLGELTKVAWEYDVQVIIEGPGHVPMQMIKENMDKQLDDCFEAPFYTLGPLTTDI
APGYDHITSGIGAAQIGWYGCAMLCYVTPKEHLGLPNKDDVKVGLITYKIAAHAADLAKGHPGAQIRDNALSKSRFEFRW
EDQFNLGLDPVTAREYHDETLPQESGKVAHFCSMCGPKFCSMKITQDVRDFTAKLDSGEISIKILDETITMTAAEGMALK
AKEFNDSGAEIYQPVL

Sequences:

>Translated_656_residues
MSNQVNSNLKTSSRKGLSRKASRRAAQDYIQNLNNESYPNSEKIFIKGELHPIKVAMRQIHQADTLLAGSGEQPVFEKNE
AIPVYDTSGPYTDPKTKIDVHAGLPKLRQSWIAARDDVETLEGVTSNFAKARLANDSLDDLRFANRPKPLRAKKGQCVTQ
MHYARRGIITPEMEYIAIRENMGRDRVKCEALHFQHAGHSFGANLQEKITPEFVRQEVARGRAIIPSNINHPELEPMIIG
RNFLVKVNSNIGNSSVSSSIEEEVEKLVWSTLWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPIYQALEKVKGVAED
LSWDVFRDTLIEQAEQGVDYFTIHAGVLLRFVPMTAKRVTGIVSRGGSIFAKWCLSHHKENFAYEYFREICEICQLYDVS
LSLGDGLRPGSVADANDQAQFAELEVLGELTKVAWEYDVQVIIEGPGHVPMQMIKENMDKQLDDCFEAPFYTLGPLTTDI
APGYDHITSGIGAAQIGWYGCAMLCYVTPKEHLGLPNKDDVKVGLITYKIAAHAADLAKGHPGAQIRDNALSKSRFEFRW
EDQFNLGLDPVTAREYHDETLPQESGKVAHFCSMCGPKFCSMKITQDVRDFTAKLDSGEISIKILDETITMTAAEGMALK
AKEFNDSGAEIYQPVL
>Mature_655_residues
SNQVNSNLKTSSRKGLSRKASRRAAQDYIQNLNNESYPNSEKIFIKGELHPIKVAMRQIHQADTLLAGSGEQPVFEKNEA
IPVYDTSGPYTDPKTKIDVHAGLPKLRQSWIAARDDVETLEGVTSNFAKARLANDSLDDLRFANRPKPLRAKKGQCVTQM
HYARRGIITPEMEYIAIRENMGRDRVKCEALHFQHAGHSFGANLQEKITPEFVRQEVARGRAIIPSNINHPELEPMIIGR
NFLVKVNSNIGNSSVSSSIEEEVEKLVWSTLWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPIYQALEKVKGVAEDL
SWDVFRDTLIEQAEQGVDYFTIHAGVLLRFVPMTAKRVTGIVSRGGSIFAKWCLSHHKENFAYEYFREICEICQLYDVSL
SLGDGLRPGSVADANDQAQFAELEVLGELTKVAWEYDVQVIIEGPGHVPMQMIKENMDKQLDDCFEAPFYTLGPLTTDIA
PGYDHITSGIGAAQIGWYGCAMLCYVTPKEHLGLPNKDDVKVGLITYKIAAHAADLAKGHPGAQIRDNALSKSRFEFRWE
DQFNLGLDPVTAREYHDETLPQESGKVAHFCSMCGPKFCSMKITQDVRDFTAKLDSGEISIKILDETITMTAAEGMALKA
KEFNDSGAEIYQPVL

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=636, Percent_Identity=73.5849056603774, Blast_Score=991, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 73223; Mature: 73092

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQVNSNLKTSSRKGLSRKASRRAAQDYIQNLNNESYPNSEKIFIKGELHPIKVAMRQI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHH
HQADTLLAGSGEQPVFEKNEAIPVYDTSGPYTDPKTKIDVHAGLPKLRQSWIAARDDVET
HHHHHEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHH
LEGVTSNFAKARLANDSLDDLRFANRPKPLRAKKGQCVTQMHYARRGIITPEMEYIAIRE
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHEEEEEC
NMGRDRVKCEALHFQHAGHSFGANLQEKITPEFVRQEVARGRAIIPSNINHPELEPMIIG
CCCCCHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHCCCEECCCCCCCCCCCEEEEC
RNFLVKVNSNIGNSSVSSSIEEEVEKLVWSTLWGGDTVMDLSTGRNIHETREWILRNSPV
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCC
PIGTVPIYQALEKVKGVAEDLSWDVFRDTLIEQAEQGVDYFTIHAGVLLRFVPMTAKRVT
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
GIVSRGGSIFAKWCLSHHKENFAYEYFREICEICQLYDVSLSLGDGLRPGSVADANDQAQ
HHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCHH
FAELEVLGELTKVAWEYDVQVIIEGPGHVPMQMIKENMDKQLDDCFEAPFYTLGPLTTDI
HHHHHHHHHHHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
APGYDHITSGIGAAQIGWYGCAMLCYVTPKEHLGLPNKDDVKVGLITYKIAAHAADLAKG
CCCHHHHHCCCCHHHHHHHHHHHHEEECCHHHCCCCCCCCEEEEEEEEHHHHHHHHHHCC
HPGAQIRDNALSKSRFEFRWEDQFNLGLDPVTAREYHDETLPQESGKVAHFCSMCGPKFC
CCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCC
SMKITQDVRDFTAKLDSGEISIKILDETITMTAAEGMALKAKEFNDSGAEIYQPVL
CCHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCEEEECCCCCCCHHHHCCCC
>Mature Secondary Structure 
SNQVNSNLKTSSRKGLSRKASRRAAQDYIQNLNNESYPNSEKIFIKGELHPIKVAMRQI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHH
HQADTLLAGSGEQPVFEKNEAIPVYDTSGPYTDPKTKIDVHAGLPKLRQSWIAARDDVET
HHHHHEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHH
LEGVTSNFAKARLANDSLDDLRFANRPKPLRAKKGQCVTQMHYARRGIITPEMEYIAIRE
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHEEEEEC
NMGRDRVKCEALHFQHAGHSFGANLQEKITPEFVRQEVARGRAIIPSNINHPELEPMIIG
CCCCCHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHCCCEECCCCCCCCCCCEEEEC
RNFLVKVNSNIGNSSVSSSIEEEVEKLVWSTLWGGDTVMDLSTGRNIHETREWILRNSPV
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCC
PIGTVPIYQALEKVKGVAEDLSWDVFRDTLIEQAEQGVDYFTIHAGVLLRFVPMTAKRVT
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
GIVSRGGSIFAKWCLSHHKENFAYEYFREICEICQLYDVSLSLGDGLRPGSVADANDQAQ
HHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCHH
FAELEVLGELTKVAWEYDVQVIIEGPGHVPMQMIKENMDKQLDDCFEAPFYTLGPLTTDI
HHHHHHHHHHHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
APGYDHITSGIGAAQIGWYGCAMLCYVTPKEHLGLPNKDDVKVGLITYKIAAHAADLAKG
CCCHHHHHCCCCHHHHHHHHHHHHEEECCHHHCCCCCCCCEEEEEEEEHHHHHHHHHHCC
HPGAQIRDNALSKSRFEFRWEDQFNLGLDPVTAREYHDETLPQESGKVAHFCSMCGPKFC
CCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCC
SMKITQDVRDFTAKLDSGEISIKILDETITMTAAEGMALKAKEFNDSGAEIYQPVL
CCHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCEEEECCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA