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The map label for this gene is cysG

Identifier: 119944083

GI number: 119944083

Start: 387379

End: 388797

Strand: Direct

Name: cysG

Synonym: Ping_0298

Alternate gene names: 119944083

Gene position: 387379-388797 (Clockwise)

Preceding gene: 119944082

Following gene: 119944086

Centisome position: 8.5

GC content: 45.45

Gene sequence:

>1419_bases
ATGGATTACCTGCCAATTTTTACCAAACTTGAAAACCGCCCATGCCTTGTTGTTGGCGGAGGAAGCATTGCTTGCCGAAA
AATTCATTTATTGCTGAAAGCGGGAGCCGATGTGACAGTCTGCGCGCTGGAATTTAACCCCTCTTTATTAAAACAAGCTG
CTAATAAGGAGTTGAAAATACTTGAGCAGGCATTTACTGAAGAGCTTCTGGAAAATAAATGGTTGGTCATTGCAGCGACC
AATAAGAAACAGGTCAATGAGCATATCGCCACGGCCGCCCATGCAAAACAATTATTAGTTAATGTGGTGGGTCAAGCTGA
TATTTCGAGCTTTATTCTTCCCTCAATTGTCGATCGCTCACCATTAGTTGTGGCTATTTCAAGCGGTGGTAAAGCCCCGG
TTCTGGCGCGCTTGATTCGTGAACGTTTAGAAACGTTATTACCCATGCATTTGGGTCGATTAGCGGCTATTTCTGCACAA
TTCCGTCACCGTGTTAAAGAAGTTATTAAAGTTGCTCCCTTGCGCAGGCGCTATTGGGAAAAACTTTTTGGCAATGGAAT
GCTTGCTAACCTATTGCAAAAAGGCCAAACAGAGAAGGCAATTGCTTTAATGGAGACGAGTCTGAGCGAAGATATTACTC
AGGGAGATGTGGCTTTAGTTGGTGCCGGGCCTGGCGACCCCTCTCTTCTAACCTTAAAAGCATTACAATTGATGCAGCAG
GCAGATGTTGTTTTATATGATCGTTTGGTTTCTTCAGATATTTTGGACTTAGTACGTCGTGATGCAGATTTAATTTCCGT
GGGCAAAGCTGCCGGTAATCACGAAGTAGAGCAGTCTCGAACTAATCAGATGTTAGTGGAATTTGCTCGAGAGGGTAAAA
AAGTGGTGCGCCTTAAAGGGGGAGATTCATTTATTTTTGGACGGGGAGGCGAAGAGCTTGAAGAGTTGGTTGAAGCAGGT
ATTGCTTTTCAGGTTGTTCCCGGTATTACCGCAGCCTCGGGTTGCAGTGCTTATGCGGGTATTCCCTTAACCCACCGCGA
TTTTGCGCAGTCAGTGACCTTTGTGACCGGACACCGCAAAACTGACGGTGAGGAGCTTAACTGGCAGGCATTAGCGGCTC
CTAACCAAACTTTGGTTGTTTATATGGGATTGATTCAGTCACAGGAAATTCAAACACAATTGCTCAGTCATGGGCGAGCC
CCTGAAACCCCTGTCGCATTGGTTAATAAGGGAACGACTTCAGATCAACATGTGGTGATTGGTCAATTATCTGAATTGGA
ACAACTGGGCGGGGGGTTACAAGGCCCAACCCTGATGATCATTGGCGAGGTCGTTAATTTGGCTGATAAACTCGCATGGT
ACCAGTCTGATAATAAACCCCCGCTTTCAAGGGATCCCTTTTTAGTAAACTTAGCTTAG

Upstream 100 bases:

>100_bases
ATATTTTGAAATAACAAATCTTATTTTAGTGTTTCATCTGTGTCAATTAATTCTCTATAGTGTCGATATCGATCGAAAAT
TACATGAATAGAGTGCGACT

Downstream 100 bases:

>100_bases
GATTAATTGTTATAGCAAACTATATCAAAACGACTTAAAGATACAGAAATCTAGATCTTCAAGTCGTTTGGGCATTTGTT
ATTTAAAAAAGAGCTAACAG

Product: fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase and uroporphyrinogen methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MDYLPIFTKLENRPCLVVGGGSIACRKIHLLLKAGADVTVCALEFNPSLLKQAANKELKILEQAFTEELLENKWLVIAAT
NKKQVNEHIATAAHAKQLLVNVVGQADISSFILPSIVDRSPLVVAISSGGKAPVLARLIRERLETLLPMHLGRLAAISAQ
FRHRVKEVIKVAPLRRRYWEKLFGNGMLANLLQKGQTEKAIALMETSLSEDITQGDVALVGAGPGDPSLLTLKALQLMQQ
ADVVLYDRLVSSDILDLVRRDADLISVGKAAGNHEVEQSRTNQMLVEFAREGKKVVRLKGGDSFIFGRGGEELEELVEAG
IAFQVVPGITAASGCSAYAGIPLTHRDFAQSVTFVTGHRKTDGEELNWQALAAPNQTLVVYMGLIQSQEIQTQLLSHGRA
PETPVALVNKGTTSDQHVVIGQLSELEQLGGGLQGPTLMIIGEVVNLADKLAWYQSDNKPPLSRDPFLVNLA

Sequences:

>Translated_472_residues
MDYLPIFTKLENRPCLVVGGGSIACRKIHLLLKAGADVTVCALEFNPSLLKQAANKELKILEQAFTEELLENKWLVIAAT
NKKQVNEHIATAAHAKQLLVNVVGQADISSFILPSIVDRSPLVVAISSGGKAPVLARLIRERLETLLPMHLGRLAAISAQ
FRHRVKEVIKVAPLRRRYWEKLFGNGMLANLLQKGQTEKAIALMETSLSEDITQGDVALVGAGPGDPSLLTLKALQLMQQ
ADVVLYDRLVSSDILDLVRRDADLISVGKAAGNHEVEQSRTNQMLVEFAREGKKVVRLKGGDSFIFGRGGEELEELVEAG
IAFQVVPGITAASGCSAYAGIPLTHRDFAQSVTFVTGHRKTDGEELNWQALAAPNQTLVVYMGLIQSQEIQTQLLSHGRA
PETPVALVNKGTTSDQHVVIGQLSELEQLGGGLQGPTLMIIGEVVNLADKLAWYQSDNKPPLSRDPFLVNLA
>Mature_472_residues
MDYLPIFTKLENRPCLVVGGGSIACRKIHLLLKAGADVTVCALEFNPSLLKQAANKELKILEQAFTEELLENKWLVIAAT
NKKQVNEHIATAAHAKQLLVNVVGQADISSFILPSIVDRSPLVVAISSGGKAPVLARLIRERLETLLPMHLGRLAAISAQ
FRHRVKEVIKVAPLRRRYWEKLFGNGMLANLLQKGQTEKAIALMETSLSEDITQGDVALVGAGPGDPSLLTLKALQLMQQ
ADVVLYDRLVSSDILDLVRRDADLISVGKAAGNHEVEQSRTNQMLVEFAREGKKVVRLKGGDSFIFGRGGEELEELVEAG
IAFQVVPGITAASGCSAYAGIPLTHRDFAQSVTFVTGHRKTDGEELNWQALAAPNQTLVVYMGLIQSQEIQTQLLSHGRA
PETPVALVNKGTTSDQHVVIGQLSELEQLGGGLQGPTLMIIGEVVNLADKLAWYQSDNKPPLSRDPFLVNLA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=460, Percent_Identity=56.304347826087, Blast_Score=496, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6322922, Length=236, Percent_Identity=36.0169491525424, Blast_Score=133, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG_PSYIN (A1SRP9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941763.1
- ProteinModelPortal:   A1SRP9
- SMR:   A1SRP9
- STRING:   A1SRP9
- GeneID:   4623717
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0298
- eggNOG:   COG0007
- HOGENOM:   HBG730212
- OMA:   MCRRDAE
- PhylomeDB:   A1SRP9
- BioCyc:   PING357804:PING_0298-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 51194; Mature: 51194

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLPIFTKLENRPCLVVGGGSIACRKIHLLLKAGADVTVCALEFNPSLLKQAANKELKI
CCCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCHHHHH
LEQAFTEELLENKWLVIAATNKKQVNEHIATAAHAKQLLVNVVGQADISSFILPSIVDRS
HHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
PLVVAISSGGKAPVLARLIRERLETLLPMHLGRLAAISAQFRHRVKEVIKVAPLRRRYWE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFGNGMLANLLQKGQTEKAIALMETSLSEDITQGDVALVGAGPGDPSLLTLKALQLMQQ
HHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
ADVVLYDRLVSSDILDLVRRDADLISVGKAAGNHEVEQSRTNQMLVEFAREGKKVVRLKG
CCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GDSFIFGRGGEELEELVEAGIAFQVVPGITAASGCSAYAGIPLTHRDFAQSVTFVTGHRK
CCEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHEEEEECCCC
TDGEELNWQALAAPNQTLVVYMGLIQSQEIQTQLLSHGRAPETPVALVNKGTTSDQHVVI
CCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEE
GQLSELEQLGGGLQGPTLMIIGEVVNLADKLAWYQSDNKPPLSRDPFLVNLA
HHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MDYLPIFTKLENRPCLVVGGGSIACRKIHLLLKAGADVTVCALEFNPSLLKQAANKELKI
CCCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCHHHHH
LEQAFTEELLENKWLVIAATNKKQVNEHIATAAHAKQLLVNVVGQADISSFILPSIVDRS
HHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
PLVVAISSGGKAPVLARLIRERLETLLPMHLGRLAAISAQFRHRVKEVIKVAPLRRRYWE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFGNGMLANLLQKGQTEKAIALMETSLSEDITQGDVALVGAGPGDPSLLTLKALQLMQQ
HHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
ADVVLYDRLVSSDILDLVRRDADLISVGKAAGNHEVEQSRTNQMLVEFAREGKKVVRLKG
CCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GDSFIFGRGGEELEELVEAGIAFQVVPGITAASGCSAYAGIPLTHRDFAQSVTFVTGHRK
CCEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHEEEEECCCC
TDGEELNWQALAAPNQTLVVYMGLIQSQEIQTQLLSHGRAPETPVALVNKGTTSDQHVVI
CCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEE
GQLSELEQLGGGLQGPTLMIIGEVVNLADKLAWYQSDNKPPLSRDPFLVNLA
HHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA