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The map label for this gene is mdh

Identifier: 119944082

GI number: 119944082

Start: 386153

End: 387112

Strand: Direct

Name: mdh

Synonym: Ping_0297

Alternate gene names: 119944082

Gene position: 386153-387112 (Clockwise)

Preceding gene: 119944081

Following gene: 119944083

Centisome position: 8.47

GC content: 40.21

Gene sequence:

>960_bases
ATGAAAATTGCTGTTCTTGGAGCCGCCGGTGGTATTGGGCAAGCACTTGCTTTATTGTTAAAACTTGATTTACCTGCAAA
TTCAATTTTATCACTTTATGATCTTGCTCCGGTTACACCTGGAGTTGCAAAAGATTTAAGCCACATCCCAACAAGTGTTA
AAGTTGAAGGTTTTGCCGGTACAGATCCTACTGCAGCTATTGAAGGTGCCGATGTTATTTTGATTTCAGCAGGAGTTGCC
CGTAAACCGGGTATGGATCGTTCTGATTTATTTAATATTAATGCTGGTATTATTAAAAACTTAGTTGCTAAGGTTGCAGA
AGTCGCACCGGAAGCTTGTATTGGAATCATTACCAATCCTGTTAATACAACAGTCGCAATTGCTGCTGAAGTGTTAAAAG
CTGCAGGTGTTTACAATAAAAACAAATTATTTGGTATTACAACCTTAGATGTTATTCGTGCCGAAGAGTTTGTCGCTGAG
TTAAAAGGTTTACCTTCTGAAGATGTTCGAGTCAATGTTATTGGTGGTCACAGCGGCACTACTATCTTGCCAGTTTTATC
GCAGATTCAGGGCGTTTCATTTACGGATCAGGAAGTGATCGACTTAACTTCTCGCATCCAAAATGCCGGCACAGAAGTGG
TTGAAGCAAAAGCAGGCGGTGGTTCTGCAACGCTTTCGATGGCCTGTGCAGCTTCTCGCTTTGCTATTCAGCTTGTTCAC
GCTTTGTCAGGAAAACAGGGTATTATTCAAAATGCCTATGTAGACGGTGGCAATCCAGCTTCTCCTTTCTTTACTCAACC
ACTATTATTGGGTAAAAATGGCATTGATAAGGTATTACCTTATGGTGAATTGAGTTCTTTCGAAGAGACCAAAAAAGAAG
AAATGTTATCTGTATTACATGCTGATATTGAAATGGGTATTGATTTTGTTAGCAAAACGCTTGCAGCTGAAGTGAGCTAA

Upstream 100 bases:

>100_bases
ATCGCTCACAATTCATCAATATTTATTACATGCTTGCTGATTTTTCATTAAAATGACCATTCACATTTATATCATTAATT
AGATACATATTGGAGTCGTT

Downstream 100 bases:

>100_bases
GCAGCACGTCAGTAGTCAATTTTTTCTTAATGCCATCACTCCAGAGTCGATGGCATTTTTTTAACCTCAATTTATTGATC
AACAGAACCTACGCTTCTTC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKIAVLGAAGGIGQALALLLKLDLPANSILSLYDLAPVTPGVAKDLSHIPTSVKVEGFAGTDPTAAIEGADVILISAGVA
RKPGMDRSDLFNINAGIIKNLVAKVAEVAPEACIGIITNPVNTTVAIAAEVLKAAGVYNKNKLFGITTLDVIRAEEFVAE
LKGLPSEDVRVNVIGGHSGTTILPVLSQIQGVSFTDQEVIDLTSRIQNAGTEVVEAKAGGGSATLSMACAASRFAIQLVH
ALSGKQGIIQNAYVDGGNPASPFFTQPLLLGKNGIDKVLPYGELSSFEETKKEEMLSVLHADIEMGIDFVSKTLAAEVS

Sequences:

>Translated_319_residues
MKIAVLGAAGGIGQALALLLKLDLPANSILSLYDLAPVTPGVAKDLSHIPTSVKVEGFAGTDPTAAIEGADVILISAGVA
RKPGMDRSDLFNINAGIIKNLVAKVAEVAPEACIGIITNPVNTTVAIAAEVLKAAGVYNKNKLFGITTLDVIRAEEFVAE
LKGLPSEDVRVNVIGGHSGTTILPVLSQIQGVSFTDQEVIDLTSRIQNAGTEVVEAKAGGGSATLSMACAASRFAIQLVH
ALSGKQGIIQNAYVDGGNPASPFFTQPLLLGKNGIDKVLPYGELSSFEETKKEEMLSVLHADIEMGIDFVSKTLAAEVS
>Mature_319_residues
MKIAVLGAAGGIGQALALLLKLDLPANSILSLYDLAPVTPGVAKDLSHIPTSVKVEGFAGTDPTAAIEGADVILISAGVA
RKPGMDRSDLFNINAGIIKNLVAKVAEVAPEACIGIITNPVNTTVAIAAEVLKAAGVYNKNKLFGITTLDVIRAEEFVAE
LKGLPSEDVRVNVIGGHSGTTILPVLSQIQGVSFTDQEVIDLTSRIQNAGTEVVEAKAGGGSATLSMACAASRFAIQLVH
ALSGKQGIIQNAYVDGGNPASPFFTQPLLLGKNGIDKVLPYGELSSFEETKKEEMLSVLHADIEMGIDFVSKTLAAEVS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family

Homologues:

Organism=Homo sapiens, GI21735621, Length=315, Percent_Identity=54.6031746031746, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI47059044, Length=297, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136809, Length=297, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789632, Length=312, Percent_Identity=71.1538461538462, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17554310, Length=312, Percent_Identity=52.2435897435897, Blast_Score=295, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6322765, Length=316, Percent_Identity=49.3670886075949, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6320125, Length=340, Percent_Identity=37.6470588235294, Blast_Score=182, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6324446, Length=367, Percent_Identity=35.9673024523161, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24647881, Length=312, Percent_Identity=52.2435897435897, Blast_Score=298, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24663595, Length=306, Percent_Identity=42.8104575163399, Blast_Score=243, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=45.6869009584665, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_PSYIN (A1SRP8)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941762.1
- ProteinModelPortal:   A1SRP8
- SMR:   A1SRP8
- STRING:   A1SRP8
- GeneID:   4623716
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0297
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   ECTYVEG
- PhylomeDB:   A1SRP8
- ProtClustDB:   PRK05086
- BioCyc:   PING357804:PING_0297-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01516
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11540:SF1
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01772

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 32997; Mature: 32997

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00068 MDH; PS00606 B_KETOACYL_SYNTHASE

Important sites: ACT_SITE 177-177 BINDING 34-34 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 153-153 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLGAAGGIGQALALLLKLDLPANSILSLYDLAPVTPGVAKDLSHIPTSVKVEGFAG
CEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCC
TDPTAAIEGADVILISAGVARKPGMDRSDLFNINAGIIKNLVAKVAEVAPEACIGIITNP
CCCCCEECCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHEECCC
VNTTVAIAAEVLKAAGVYNKNKLFGITTLDVIRAEEFVAELKGLPSEDVRVNVIGGHSGT
CHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
TILPVLSQIQGVSFTDQEVIDLTSRIQNAGTEVVEAKAGGGSATLSMACAASRFAIQLVH
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALSGKQGIIQNAYVDGGNPASPFFTQPLLLGKNGIDKVLPYGELSSFEETKKEEMLSVLH
HHCCCCCCCEECEECCCCCCCCCCCCCHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
ADIEMGIDFVSKTLAAEVS
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIAVLGAAGGIGQALALLLKLDLPANSILSLYDLAPVTPGVAKDLSHIPTSVKVEGFAG
CEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCC
TDPTAAIEGADVILISAGVARKPGMDRSDLFNINAGIIKNLVAKVAEVAPEACIGIITNP
CCCCCEECCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHEECCC
VNTTVAIAAEVLKAAGVYNKNKLFGITTLDVIRAEEFVAELKGLPSEDVRVNVIGGHSGT
CHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
TILPVLSQIQGVSFTDQEVIDLTSRIQNAGTEVVEAKAGGGSATLSMACAASRFAIQLVH
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALSGKQGIIQNAYVDGGNPASPFFTQPLLLGKNGIDKVLPYGELSSFEETKKEEMLSVLH
HHCCCCCCCEECEECCCCCCCCCCCCCHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
ADIEMGIDFVSKTLAAEVS
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA