Click here to switch to the map view.

The map label for this gene is hoxA [H]

Identifier: 119900094

GI number: 119900094

Start: 4164280

End: 4165737

Strand: Direct

Name: hoxA [H]

Synonym: azo3805

Alternate gene names: 119900094

Gene position: 4164280-4165737 (Clockwise)

Preceding gene: 119900093

Following gene: 119900096

Centisome position: 95.16

GC content: 69.82

Gene sequence:

>1458_bases
ATGCCCCCGCCGCTGCCGCCCGAACTGCCGTCCATCCTCGTCGTCGACGACGAGATCCGCTCGCAGGAAGCCCTGCGCCG
CACGCTGGAGGAAGACTTCGAAGTCTTCACCGCGTCCGGTGCCGACGACGCGATCGCCATCCTCGAGCGCGAATGGATAC
AGATCGTGCTGTGCGACCAGCGCATGCCGGGCAGCTCCGGCGTCGCGCTGCTGCGCCAGGTGCGCGAGCGCTGGCCCGAG
GCGGTGCGCATCATCATTTCCGGCTACACCGATTCGGAAGACATCATCGCCGGCATCAACGAGGCCGGCATCTACCAGTA
CCTGCTCAAGCCCTGGCAGCCGGAACAGCTGCTGCTGGCGCTGAAATCCGCCGCCGAGATGGCCCGGCTGCACGCCGAGA
ACCAGCGCCTGACGCTGGAACTGCGCACCGCCGCGCCGGTGCTCGAAAAACAGGTCAGCCACCGGCGCGCCAGCGTGCGC
CAGCAGTTCGCGCTCGACGCCGTGCTGCGCGCGCCCGGCTCGCCGATGAACGCGGTGTGCGCGCTGGTGAAAAAGCTCGC
CGCGCTCGACATCCCGGTGCTGCTGACCGGCGAATCCGGCACCGGCAAGGAACTGCTGGCGCGCGCGCTGCACTACGACA
GCCCGCGCGCCGGCGAGGCCTTCGTGGTGGAGAACTGCGGCGCGCTGCCCGACCAGCTGCTGGAATCCGAACTCTTCGGC
CACAAGCGCGGCGCCTTCACCGGCGCCTTCGAAGACCGCGTCGGCCTTTTCAAGCAGGCCGACGGCGGCACCATGCTGCT
CGACGAGATCGGTGAAACCTCGTTCGCCTTCCAGGTGAAGCTGCTGCGCGCGCTGCAGGAAGGCGAGGTGCGGCCGGTGG
GAGCGCCGCGGCCGATTCCGGTGGATGCGCGGGTGATTGCCGCCACCAACCGCGACCTCGAAGCCGAGGTGCGCGCTGGC
CGCTTCCGCGAGGATCTCTACTACCGGCTGGCGGCACTCACCATCCATGTGCCGCCGCTGCGCGAGCGCACGATGGACAT
CCCGCTGATCGCGCAGGCGCTGGTGGATGAAACCCAGGCCGCACTCGGGCGCCGCTTCGAGCCGCTGTCGGCCGAGGTGA
TCACCTGCCTGCAGGCCTGGCGCTGGCCGGGCAATGTGCGCGAACTGCGCAACGAGGTGCTGCGCATGATCGCGCTCGCC
GACGACGAGCGCCTGTCGGCCGCCCACCTGAGCCCGCGCGTGCTGCGTGCGGGCGACGCGCACGAGGAGCCGGCGCTGTC
GATGCTGAGCGGGCTGGACGGCGACCTCAAGACCCGGCTGGAAGCGCTCGAAGCGCGCATCGTCAAGGAAAGCCTGATCC
GCCACCGCTGGAACAAGACCCGCGCGGCTAAGGAACTCGGCCTGTCGCGCGTCGGCCTGCGCAGCAAGCTGGCGCGCTAT
GGGCTGGAGCGCGACTGA

Upstream 100 bases:

>100_bases
CACCACTTCGTGCAGTTGCGCACCGCGCTCGGCGGCATGCGCATGCTCGACTGGCTGTCGGGCGAACCGCTGCCGCGCAT
CTGCTGAGCGCCGCCGACCG

Downstream 100 bases:

>100_bases
AGCCGCGCGGCCACCTCAGCCGCGCAGGCCGCGGCGCTCATCCACCTCGGCGAGCGCACGCAGGATGAAGGCAGAGGTCG
GCCCGACGCCTTCGCTTTCC

Product: hydrogenase transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MPPPLPPELPSILVVDDEIRSQEALRRTLEEDFEVFTASGADDAIAILEREWIQIVLCDQRMPGSSGVALLRQVRERWPE
AVRIIISGYTDSEDIIAGINEAGIYQYLLKPWQPEQLLLALKSAAEMARLHAENQRLTLELRTAAPVLEKQVSHRRASVR
QQFALDAVLRAPGSPMNAVCALVKKLAALDIPVLLTGESGTGKELLARALHYDSPRAGEAFVVENCGALPDQLLESELFG
HKRGAFTGAFEDRVGLFKQADGGTMLLDEIGETSFAFQVKLLRALQEGEVRPVGAPRPIPVDARVIAATNRDLEAEVRAG
RFREDLYYRLAALTIHVPPLRERTMDIPLIAQALVDETQAALGRRFEPLSAEVITCLQAWRWPGNVRELRNEVLRMIALA
DDERLSAAHLSPRVLRAGDAHEEPALSMLSGLDGDLKTRLEALEARIVKESLIRHRWNKTRAAKELGLSRVGLRSKLARY
GLERD

Sequences:

>Translated_485_residues
MPPPLPPELPSILVVDDEIRSQEALRRTLEEDFEVFTASGADDAIAILEREWIQIVLCDQRMPGSSGVALLRQVRERWPE
AVRIIISGYTDSEDIIAGINEAGIYQYLLKPWQPEQLLLALKSAAEMARLHAENQRLTLELRTAAPVLEKQVSHRRASVR
QQFALDAVLRAPGSPMNAVCALVKKLAALDIPVLLTGESGTGKELLARALHYDSPRAGEAFVVENCGALPDQLLESELFG
HKRGAFTGAFEDRVGLFKQADGGTMLLDEIGETSFAFQVKLLRALQEGEVRPVGAPRPIPVDARVIAATNRDLEAEVRAG
RFREDLYYRLAALTIHVPPLRERTMDIPLIAQALVDETQAALGRRFEPLSAEVITCLQAWRWPGNVRELRNEVLRMIALA
DDERLSAAHLSPRVLRAGDAHEEPALSMLSGLDGDLKTRLEALEARIVKESLIRHRWNKTRAAKELGLSRVGLRSKLARY
GLERD
>Mature_484_residues
PPPLPPELPSILVVDDEIRSQEALRRTLEEDFEVFTASGADDAIAILEREWIQIVLCDQRMPGSSGVALLRQVRERWPEA
VRIIISGYTDSEDIIAGINEAGIYQYLLKPWQPEQLLLALKSAAEMARLHAENQRLTLELRTAAPVLEKQVSHRRASVRQ
QFALDAVLRAPGSPMNAVCALVKKLAALDIPVLLTGESGTGKELLARALHYDSPRAGEAFVVENCGALPDQLLESELFGH
KRGAFTGAFEDRVGLFKQADGGTMLLDEIGETSFAFQVKLLRALQEGEVRPVGAPRPIPVDARVIAATNRDLEAEVRAGR
FREDLYYRLAALTIHVPPLRERTMDIPLIAQALVDETQAALGRRFEPLSAEVITCLQAWRWPGNVRELRNEVLRMIALAD
DERLSAAHLSPRVLRAGDAHEEPALSMLSGLDGDLKTRLEALEARIVKESLIRHRWNKTRAAKELGLSRVGLRSKLARYG
LERD

Specific function: Probable member of the two-component regulatory system involved in the regulation of the hydrogenase activity. HoxA is probably phosphorylated by a sensory component (which could be hoxX) and then acts in conjunction with sigma-54 as a transcriptional act

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=482, Percent_Identity=36.9294605809129, Blast_Score=287, Evalue=9e-79,
Organism=Escherichia coli, GI1790437, Length=469, Percent_Identity=37.5266524520256, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI1788905, Length=479, Percent_Identity=35.9081419624217, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1790299, Length=488, Percent_Identity=33.6065573770492, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1789233, Length=307, Percent_Identity=40.7166123778502, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=41.875, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789087, Length=310, Percent_Identity=43.2258064516129, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI87082117, Length=307, Percent_Identity=43.3224755700326, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1787583, Length=309, Percent_Identity=39.4822006472492, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081872, Length=306, Percent_Identity=40.5228758169935, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1786524, Length=307, Percent_Identity=39.7394136807818, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789828, Length=316, Percent_Identity=36.3924050632911, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=32, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53877; Mature: 53745

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPLPPELPSILVVDDEIRSQEALRRTLEEDFEVFTASGADDAIAILEREWIQIVLCDQ
CCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEEEEEECC
RMPGSSGVALLRQVRERWPEAVRIIISGYTDSEDIIAGINEAGIYQYLLKPWQPEQLLLA
CCCCCCHHHHHHHHHHHHHHHHEEHEECCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHH
LKSAAEMARLHAENQRLTLELRTAAPVLEKQVSHRRASVRQQFALDAVLRAPGSPMNAVC
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALVKKLAALDIPVLLTGESGTGKELLARALHYDSPRAGEAFVVENCGALPDQLLESELFG
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHC
HKRGAFTGAFEDRVGLFKQADGGTMLLDEIGETSFAFQVKLLRALQEGEVRPVGAPRPIP
CCCCCCCCCHHHHHHHHEECCCCEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
VDARVIAATNRDLEAEVRAGRFREDLYYRLAALTIHVPPLRERTMDIPLIAQALVDETQA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCCCCHHHHHHHHHHHHH
ALGRRFEPLSAEVITCLQAWRWPGNVRELRNEVLRMIALADDERLSAAHLSPRVLRAGDA
HHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHCCCCC
HEEPALSMLSGLDGDLKTRLEALEARIVKESLIRHRWNKTRAAKELGLSRVGLRSKLARY
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
GLERD
CCCCC
>Mature Secondary Structure 
PPPLPPELPSILVVDDEIRSQEALRRTLEEDFEVFTASGADDAIAILEREWIQIVLCDQ
CCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEEEEEECC
RMPGSSGVALLRQVRERWPEAVRIIISGYTDSEDIIAGINEAGIYQYLLKPWQPEQLLLA
CCCCCCHHHHHHHHHHHHHHHHEEHEECCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHH
LKSAAEMARLHAENQRLTLELRTAAPVLEKQVSHRRASVRQQFALDAVLRAPGSPMNAVC
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALVKKLAALDIPVLLTGESGTGKELLARALHYDSPRAGEAFVVENCGALPDQLLESELFG
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHC
HKRGAFTGAFEDRVGLFKQADGGTMLLDEIGETSFAFQVKLLRALQEGEVRPVGAPRPIP
CCCCCCCCCHHHHHHHHEECCCCEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
VDARVIAATNRDLEAEVRAGRFREDLYYRLAALTIHVPPLRERTMDIPLIAQALVDETQA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCCCCHHHHHHHHHHHHH
ALGRRFEPLSAEVITCLQAWRWPGNVRELRNEVLRMIALADDERLSAAHLSPRVLRAGDA
HHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHCCCCC
HEEPALSMLSGLDGDLKTRLEALEARIVKESLIRHRWNKTRAAKELGLSRVGLRSKLARY
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
GLERD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2001989; 12948488 [H]