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The map label for this gene is hypE [H]

Identifier: 119900093

GI number: 119900093

Start: 4163214

End: 4164266

Strand: Direct

Name: hypE [H]

Synonym: azo3804

Alternate gene names: 119900093

Gene position: 4163214-4164266 (Clockwise)

Preceding gene: 119900092

Following gene: 119900094

Centisome position: 95.14

GC content: 72.17

Gene sequence:

>1053_bases
ATGAAAGCCAACTACACCCGCCCGCTGGACCTCAAGCATGGCCGCGTCGACATGAACCACGGCGCCGGCGGCCGCGCCAC
CGCGCAGCTGGTCTCCGAACTCTTCGCCCGCGCCTTCGACAACCCCTGGCTCGCCCAGGGCGACGACGGCGCAGTGCTGC
CGGCGCCCGAACCCGGCGAACGCCTGGTGATGGCGACCGACGGCCACGTGGTGTCGCCGCTGTTCTTTCCCGGCGGCGAC
ATCGGCAGCCTGGCAGTGCACGGCACGGTCAACGACCTCGCGGTGATGGGCGCGCGCCCGCTCTACCTGTCCGCGAGCTT
CATCCTGGAAGAAGGCTATCCGCTCGCCGACCTCAAACGCATCGTCGACGCCATGGCCGAGGCCAGCCGCAGCGTCGGTG
TGCCGGTGGTCACCGGCGACACCAAGGTGGTCGAGCAGGGCAAGGGCGACGGCGTCTTCATTGCCACCACTGGCGTCGGC
GCGCTGCCCCCGGGCCGGGCGCCGGGTGGCGCCAGCGCGCGCCCGGGCGACGTGGTGCTGGTGTCGGGCAGCATGGGCGA
CCACGGCATGGCCATCCTCGCGGTGCGCGAGGCGCTCGCCTTCGACAGCGAAATCCGCTCCGACAGCGCACCGCTCGCCT
GCCTGGTCGAAACGCTGTACGCCGCGGTGCCGGCCGACGCGATCCGCGTGCTGCGCGATCCCACCCGTGGCGGGCTGGCC
ACCACGCTCAACGAGATCGCGCGCCAGTCCGGCGCCGGCATGCTGATCGACGAGGCCGCGATCCCGGTGCATCCGCAGGT
GGAAGCCGCCTGCGAGCTGCTCGGGCTGGACCCGCTCTATCTCGCCAACGAGGGCAAGCTGATCGTCGTCTGCGCGCCGG
AACACGCCGAGGCCGCGCTCGCCGCCATGCGGGCCCATCCGCGCGGCCGGGACGCCGCCCGCATCGGCACCGTGATCGCG
GACGATCACCACTTCGTGCAGTTGCGCACCGCGCTCGGCGGCATGCGCATGCTCGACTGGCTGTCGGGCGAACCGCTGCC
GCGCATCTGCTGA

Upstream 100 bases:

>100_bases
GTTTCCGCGACATCGCGGTGGTGGCCGCCTGACGCATCCCCGCGCAGCCACGCCGGCCCGGCCGGCGCCGGCTGCGCCCC
TACCGCACCGACATCCGCCG

Downstream 100 bases:

>100_bases
GCGCCGCCGACCGATGCCCCCGCCGCTGCCGCCCGAACTGCCGTCCATCCTCGTCGTCGACGACGAGATCCGCTCGCAGG
AAGCCCTGCGCCGCACGCTG

Product: hydrogenase expression/formation protein hypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKANYTRPLDLKHGRVDMNHGAGGRATAQLVSELFARAFDNPWLAQGDDGAVLPAPEPGERLVMATDGHVVSPLFFPGGD
IGSLAVHGTVNDLAVMGARPLYLSASFILEEGYPLADLKRIVDAMAEASRSVGVPVVTGDTKVVEQGKGDGVFIATTGVG
ALPPGRAPGGASARPGDVVLVSGSMGDHGMAILAVREALAFDSEIRSDSAPLACLVETLYAAVPADAIRVLRDPTRGGLA
TTLNEIARQSGAGMLIDEAAIPVHPQVEAACELLGLDPLYLANEGKLIVVCAPEHAEAALAAMRAHPRGRDAARIGTVIA
DDHHFVQLRTALGGMRMLDWLSGEPLPRIC

Sequences:

>Translated_350_residues
MKANYTRPLDLKHGRVDMNHGAGGRATAQLVSELFARAFDNPWLAQGDDGAVLPAPEPGERLVMATDGHVVSPLFFPGGD
IGSLAVHGTVNDLAVMGARPLYLSASFILEEGYPLADLKRIVDAMAEASRSVGVPVVTGDTKVVEQGKGDGVFIATTGVG
ALPPGRAPGGASARPGDVVLVSGSMGDHGMAILAVREALAFDSEIRSDSAPLACLVETLYAAVPADAIRVLRDPTRGGLA
TTLNEIARQSGAGMLIDEAAIPVHPQVEAACELLGLDPLYLANEGKLIVVCAPEHAEAALAAMRAHPRGRDAARIGTVIA
DDHHFVQLRTALGGMRMLDWLSGEPLPRIC
>Mature_350_residues
MKANYTRPLDLKHGRVDMNHGAGGRATAQLVSELFARAFDNPWLAQGDDGAVLPAPEPGERLVMATDGHVVSPLFFPGGD
IGSLAVHGTVNDLAVMGARPLYLSASFILEEGYPLADLKRIVDAMAEASRSVGVPVVTGDTKVVEQGKGDGVFIATTGVG
ALPPGRAPGGASARPGDVVLVSGSMGDHGMAILAVREALAFDSEIRSDSAPLACLVETLYAAVPADAIRVLRDPTRGGLA
TTLNEIARQSGAGMLIDEAAIPVHPQVEAACELLGLDPLYLANEGKLIVVCAPEHAEAALAAMRAHPRGRDAARIGTVIA
DDHHFVQLRTALGGMRMLDWLSGEPLPRIC

Specific function: Unknown

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=339, Percent_Identity=48.9675516224189, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI1786619, Length=248, Percent_Identity=30.241935483871, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 36443; Mature: 36443

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKANYTRPLDLKHGRVDMNHGAGGRATAQLVSELFARAFDNPWLAQGDDGAVLPAPEPGE
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
RLVMATDGHVVSPLFFPGGDIGSLAVHGTVNDLAVMGARPLYLSASFILEEGYPLADLKR
EEEEEECCCEECEEEECCCCCCCEEEECCHHHHHHHCCCCCEEEEEEHCCCCCCHHHHHH
IVDAMAEASRSVGVPVVTGDTKVVEQGKGDGVFIATTGVGALPPGRAPGGASARPGDVVL
HHHHHHHHHHCCCCEEEECCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
VSGSMGDHGMAILAVREALAFDSEIRSDSAPLACLVETLYAAVPADAIRVLRDPTRGGLA
EECCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCHH
TTLNEIARQSGAGMLIDEAAIPVHPQVEAACELLGLDPLYLANEGKLIVVCAPEHAEAAL
HHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHH
AAMRAHPRGRDAARIGTVIADDHHFVQLRTALGGMRMLDWLSGEPLPRIC
HHHHCCCCCCCHHHHCEEEECCCCEEEEHHHHCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKANYTRPLDLKHGRVDMNHGAGGRATAQLVSELFARAFDNPWLAQGDDGAVLPAPEPGE
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
RLVMATDGHVVSPLFFPGGDIGSLAVHGTVNDLAVMGARPLYLSASFILEEGYPLADLKR
EEEEEECCCEECEEEECCCCCCCEEEECCHHHHHHHCCCCCEEEEEEHCCCCCCHHHHHH
IVDAMAEASRSVGVPVVTGDTKVVEQGKGDGVFIATTGVGALPPGRAPGGASARPGDVVL
HHHHHHHHHHCCCCEEEECCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
VSGSMGDHGMAILAVREALAFDSEIRSDSAPLACLVETLYAAVPADAIRVLRDPTRGGLA
EECCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCHH
TTLNEIARQSGAGMLIDEAAIPVHPQVEAACELLGLDPLYLANEGKLIVVCAPEHAEAAL
HHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHH
AAMRAHPRGRDAARIGTVIADDHHFVQLRTALGGMRMLDWLSGEPLPRIC
HHHHCCCCCCCHHHHCEEEECCCCEEEEHHHHCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8352644; 12948488; 8021224 [H]