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The map label for this gene is ntrB [H]

Identifier: 119867137

GI number: 119867137

Start: 1169218

End: 1170678

Strand: Reverse

Name: ntrB [H]

Synonym: Mkms_1085

Alternate gene names: 119867137

Gene position: 1170678-1169218 (Counterclockwise)

Preceding gene: 119867138

Following gene: 119867136

Centisome position: 20.4

GC content: 66.46

Gene sequence:

>1461_bases
ATGGGCGAGACATGCCTGCGCGACGAACTGCGCACCCTGTTCCTGTTCGAGGCGCTCTCCGACGAACAACTCGACATGCT
GTGCGCCGACGGTCACATCGAGACGTTCCCGGCTGGGCCGCTGTGCACCGAGGGTGACCCCGCCACCTGCTTCTACGTCC
TGATCGACGGCGAACTGACGATGTCGAAACGCTCCGGCGGGGCCGACATCCAGACCGGGCGCACCTCGCAGCGCGGGGTG
TACTGCGGGGCGTGGTCGGCGTACGTCCCCGGCGAGGAGCACGTGTACGAGGCCTCGGTGCGGCTGAACAGACCGTCGAA
GTTCTTCGTCCTCGACGCCGATGCGTTCGCCCGGTTCATGCAGTCCCAGTTCCCGATGGCCGTTCATCTGCTGGAGGGCC
ACCGCGTCGGTGGCCGCAGGCAGCATCAGATCATCGGTCAGCGCGAGAAACTCCTCGCACTGGGGACCATCACCGCCGGG
CTCACCCACCAGCTCAACAACCCCGCCGCGGCCACCGCCCGCGCCGTCGCCGACCTGCAGGAGGGTGTCGGCAAGATGCG
CCACAAGCTGGCGATGCTCGCCAGCGGCAAGTTCAGCCCCGAGGCGCTGCGGGTCCTGGTGAAGATCCAGGACGAGGTCG
CCGAACAGGTCGCGAAGTCCAGGAGCATGGACCTCACGGCGCTGGAGACCTCCGACCGCGAGGACCAGATCGGCGACTGG
CTCGACGACCACGGGATCGCCGGCGGCTGGGACTACGCCCCCACCTTCGTCGAGGCGGGTCTCGACATCGACTGGCTGGA
GCGCATCTCGGCGTCGGTCGACGACGTGATGGACGGCGACTGTTCGGCGTCGCTGCAGAGCGCGCTGGGCTGGCTGAAGT
ACACCATCGACACCGAACTGCGGATGAGCGAGATCGCCGAGGCGAGTAAACGCATCTCCGCACTGCTCGCCGGTGCCAAG
CAGTACTCCCAGATGGACCGCGGCGCCTATCAGAGCGCCAACGTCCACGAATTGATATACAGCACGATCAAGACGATCTT
CGGCGACAGGATCGGCAAGGACAAACCGGTCGGGCTGGTGTGGGAGAAGGATACGTCGCTGCCCGAATTGCTCTGCTACC
CAGGCGATCTCAACGAGGTGTGGTCCAACCTCATCGACAACGCCCTCCAAGCGATGGGCGGGCACGGCACGCTGACCGTA
CGCACCATGCGGGAGAACGACGAACGGGTGCGCGTCGAGATCTGCGACGACGGGCCCGGCATCCCGGAGCACATCGTCGA
CCGCATCTTCACCCCGTTCTTCACCACCAAACCCGTCGGGGAGGGCACCGGACTCGGCCTCGACCTGGCCCGCCGCATCA
TCGTCGAGAAGCACCACGGCGACCTGCGCGTCGAATCCGTACCGGGTGACACCCGCTTCATCGTGTGCCTGCCGCTGGTG
GCCCCCGCGCCCACTGAATAG

Upstream 100 bases:

>100_bases
GCGCGGAATCCGCCAAACGGGTGGCCGCCGCCGTCGGCGAAGGATCGATGGCGGTGATGCTGGTGCACCGGTATCTGGCG
GAAGCCTGAAAGGGGTCACG

Downstream 100 bases:

>100_bases
CCACGGAATAGTTCGCGGCGGGGTCGGTTGGAACCGGAATGACGAAACCCGATCTCGGTAGGTACGGCGTGTTCGGGCGC
GGGGTCACTCCCGCGCAGGC

Product: cyclic nucleotide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGETCLRDELRTLFLFEALSDEQLDMLCADGHIETFPAGPLCTEGDPATCFYVLIDGELTMSKRSGGADIQTGRTSQRGV
YCGAWSAYVPGEEHVYEASVRLNRPSKFFVLDADAFARFMQSQFPMAVHLLEGHRVGGRRQHQIIGQREKLLALGTITAG
LTHQLNNPAAATARAVADLQEGVGKMRHKLAMLASGKFSPEALRVLVKIQDEVAEQVAKSRSMDLTALETSDREDQIGDW
LDDHGIAGGWDYAPTFVEAGLDIDWLERISASVDDVMDGDCSASLQSALGWLKYTIDTELRMSEIAEASKRISALLAGAK
QYSQMDRGAYQSANVHELIYSTIKTIFGDRIGKDKPVGLVWEKDTSLPELLCYPGDLNEVWSNLIDNALQAMGGHGTLTV
RTMRENDERVRVEICDDGPGIPEHIVDRIFTPFFTTKPVGEGTGLGLDLARRIIVEKHHGDLRVESVPGDTRFIVCLPLV
APAPTE

Sequences:

>Translated_486_residues
MGETCLRDELRTLFLFEALSDEQLDMLCADGHIETFPAGPLCTEGDPATCFYVLIDGELTMSKRSGGADIQTGRTSQRGV
YCGAWSAYVPGEEHVYEASVRLNRPSKFFVLDADAFARFMQSQFPMAVHLLEGHRVGGRRQHQIIGQREKLLALGTITAG
LTHQLNNPAAATARAVADLQEGVGKMRHKLAMLASGKFSPEALRVLVKIQDEVAEQVAKSRSMDLTALETSDREDQIGDW
LDDHGIAGGWDYAPTFVEAGLDIDWLERISASVDDVMDGDCSASLQSALGWLKYTIDTELRMSEIAEASKRISALLAGAK
QYSQMDRGAYQSANVHELIYSTIKTIFGDRIGKDKPVGLVWEKDTSLPELLCYPGDLNEVWSNLIDNALQAMGGHGTLTV
RTMRENDERVRVEICDDGPGIPEHIVDRIFTPFFTTKPVGEGTGLGLDLARRIIVEKHHGDLRVESVPGDTRFIVCLPLV
APAPTE
>Mature_485_residues
GETCLRDELRTLFLFEALSDEQLDMLCADGHIETFPAGPLCTEGDPATCFYVLIDGELTMSKRSGGADIQTGRTSQRGVY
CGAWSAYVPGEEHVYEASVRLNRPSKFFVLDADAFARFMQSQFPMAVHLLEGHRVGGRRQHQIIGQREKLLALGTITAGL
THQLNNPAAATARAVADLQEGVGKMRHKLAMLASGKFSPEALRVLVKIQDEVAEQVAKSRSMDLTALETSDREDQIGDWL
DDHGIAGGWDYAPTFVEAGLDIDWLERISASVDDVMDGDCSASLQSALGWLKYTIDTELRMSEIAEASKRISALLAGAKQ
YSQMDRGAYQSANVHELIYSTIKTIFGDRIGKDKPVGLVWEKDTSLPELLCYPGDLNEVWSNLIDNALQAMGGHGTLTVR
TMRENDERVRVEICDDGPGIPEHIVDRIFTPFFTTKPVGEGTGLGLDLARRIIVEKHHGDLRVESVPGDTRFIVCLPLVA
PAPTE

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=152, Percent_Identity=36.8421052631579, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788549, Length=179, Percent_Identity=32.9608938547486, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790300, Length=133, Percent_Identity=39.0977443609023, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53394; Mature: 53263

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00888 CNMP_BINDING_1 ; PS50042 CNMP_BINDING_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGETCLRDELRTLFLFEALSDEQLDMLCADGHIETFPAGPLCTEGDPATCFYVLIDGELT
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCCCCEEEEEEEECEEE
MSKRSGGADIQTGRTSQRGVYCGAWSAYVPGEEHVYEASVRLNRPSKFFVLDADAFARFM
ECCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHEEEEEEEECCCCEEEEEEHHHHHHHH
QSQFPMAVHLLEGHRVGGRRQHQIIGQREKLLALGTITAGLTHQLNNPAAATARAVADLQ
HHCCCEEEEEECCCCCCCCHHHHHHCCHHHEEEEHHHHHHHHHHCCCCHHHHHHHHHHHH
EGVGKMRHKLAMLASGKFSPEALRVLVKIQDEVAEQVAKSRSMDLTALETSDREDQIGDW
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
LDDHGIAGGWDYAPTFVEAGLDIDWLERISASVDDVMDGDCSASLQSALGWLKYTIDTEL
HHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEECCHH
RMSEIAEASKRISALLAGAKQYSQMDRGAYQSANVHELIYSTIKTIFGDRIGKDKPVGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
WEKDTSLPELLCYPGDLNEVWSNLIDNALQAMGGHGTLTVRTMRENDERVRVEICDDGPG
EECCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCC
IPEHIVDRIFTPFFTTKPVGEGTGLGLDLARRIIVEKHHGDLRVESVPGDTRFIVCLPLV
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCEEEEECCCCCEEEEEEECC
APAPTE
CCCCCC
>Mature Secondary Structure 
GETCLRDELRTLFLFEALSDEQLDMLCADGHIETFPAGPLCTEGDPATCFYVLIDGELT
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCCCCEEEEEEEECEEE
MSKRSGGADIQTGRTSQRGVYCGAWSAYVPGEEHVYEASVRLNRPSKFFVLDADAFARFM
ECCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHEEEEEEEECCCCEEEEEEHHHHHHHH
QSQFPMAVHLLEGHRVGGRRQHQIIGQREKLLALGTITAGLTHQLNNPAAATARAVADLQ
HHCCCEEEEEECCCCCCCCHHHHHHCCHHHEEEEHHHHHHHHHHCCCCHHHHHHHHHHHH
EGVGKMRHKLAMLASGKFSPEALRVLVKIQDEVAEQVAKSRSMDLTALETSDREDQIGDW
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
LDDHGIAGGWDYAPTFVEAGLDIDWLERISASVDDVMDGDCSASLQSALGWLKYTIDTEL
HHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEECCHH
RMSEIAEASKRISALLAGAKQYSQMDRGAYQSANVHELIYSTIKTIFGDRIGKDKPVGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
WEKDTSLPELLCYPGDLNEVWSNLIDNALQAMGGHGTLTVRTMRENDERVRVEICDDGPG
EECCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCC
IPEHIVDRIFTPFFTTKPVGEGTGLGLDLARRIIVEKHHGDLRVESVPGDTRFIVCLPLV
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCEEEEECCCCCEEEEEEECC
APAPTE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]