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The map label for this gene is gmd [H]

Identifier: 119867015

GI number: 119867015

Start: 1049272

End: 1050327

Strand: Reverse

Name: gmd [H]

Synonym: Mkms_0963

Alternate gene names: 119867015

Gene position: 1050327-1049272 (Counterclockwise)

Preceding gene: 119867016

Following gene: 119867014

Centisome position: 18.31

GC content: 56.53

Gene sequence:

>1056_bases
ATGACTAAGCGTGCGCTCATCACAGGTATCACTGGACAGGATGGCTCATATCTTGCGGAGCTGTTACTCCACAAAGGCTA
TGAAGTACATGGCTTGATCCGCCGCGCATCAACATTCAACACCGCGCGGATCGACCACCTTTATGTCGACCCACATTCAC
CCAACGCCAAACTATTCCTGCACTACGGCGATCTCAGTGATGGTGCTCGGTTAGTGACGTTGTTAGCCAGCATAAGACCC
GACGAAGTCTACAACCTTGCTGCGCAGTCCCACGTGCGTGTTTCATTTGACGAGCCAGAACACACTGCTGACACGACCGG
AGCAGGAACCATTCGAATGCTAGAGGCGGTCCGGCTGTCCGGCATAGAAACACGTTTCTACCAGGCATCCTCGTCAGAGT
TGTATGGGGCGACTCCCCCACCACAGAACGAGGAAACTCCCTTCTATCCGCGTTCACCATATGCAGCCGCGAAGCTGTAC
AGCTATTGGATTACGAAAAATTATCGTGAGGCTTACGGCCTTTTCGCCGTCAACGGGATCTTGTTCAATCATGAATCGCC
GCGCCGCGGCGAGACCTTCGTGACAAGGAAGATAACTCGCGCCGCCGCCGCTATCAAAAGTGGTAGCCAAGATTACCTCT
ATATGGGCAACCTCGACGCGGTACGGGACTGGGGATACGCGCCTGAGTACGTCGAAGGCATGTGGCGCATGCTGCAGGTT
GACGAGCCTAACGACTATGTCCTTGCCACCGGCGTGGGAATTACGGTCCGTGATTTCCTCGAGATAGCGTTCGCGCATGT
GGGGCTGCGGTGGGAGGATCACGTCCGATTCGACGAACGCTATCTCCGCCCCACCGAGGTTGACGCGCTGATTGGAGACT
CGACGCGTGCGAGAGAGAAGCTCGGCTGGGTACCAACTGTGCATGGCCAAACGCTCGCTCGCCTGATGGTCGACGCCGAC
ATTGAAGCGCTCCAGCATGAGGGGAGCCCTTGGTTCGACAAGGTGCTCTTGGATTCGTGGGGGACTGCGCCCACTCAAGA
GGTGAGTGCGCCATGA

Upstream 100 bases:

>100_bases
GGGGCGGTCTCTTACGGGACGCGCCGACTCAGGCTACGCAGCCTACACTTGCTTCAAAAGGGAATCGCAACTTCGTTGCC
CCGTAAACCCAAAGGGACCG

Downstream 100 bases:

>100_bases
CCGCTGCGCTTCAATTTCCACCTAACGAATTGGATCGCGGCGCAAACGTTTATGTTGCCGGGCATCGTGGCCTGGTCGGC
TCCGCGATCGTGCGAAGATT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTKRALITGITGQDGSYLAELLLHKGYEVHGLIRRASTFNTARIDHLYVDPHSPNAKLFLHYGDLSDGARLVTLLASIRP
DEVYNLAAQSHVRVSFDEPEHTADTTGAGTIRMLEAVRLSGIETRFYQASSSELYGATPPPQNEETPFYPRSPYAAAKLY
SYWITKNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAAAAIKSGSQDYLYMGNLDAVRDWGYAPEYVEGMWRMLQV
DEPNDYVLATGVGITVRDFLEIAFAHVGLRWEDHVRFDERYLRPTEVDALIGDSTRAREKLGWVPTVHGQTLARLMVDAD
IEALQHEGSPWFDKVLLDSWGTAPTQEVSAP

Sequences:

>Translated_351_residues
MTKRALITGITGQDGSYLAELLLHKGYEVHGLIRRASTFNTARIDHLYVDPHSPNAKLFLHYGDLSDGARLVTLLASIRP
DEVYNLAAQSHVRVSFDEPEHTADTTGAGTIRMLEAVRLSGIETRFYQASSSELYGATPPPQNEETPFYPRSPYAAAKLY
SYWITKNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAAAAIKSGSQDYLYMGNLDAVRDWGYAPEYVEGMWRMLQV
DEPNDYVLATGVGITVRDFLEIAFAHVGLRWEDHVRFDERYLRPTEVDALIGDSTRAREKLGWVPTVHGQTLARLMVDAD
IEALQHEGSPWFDKVLLDSWGTAPTQEVSAP
>Mature_350_residues
TKRALITGITGQDGSYLAELLLHKGYEVHGLIRRASTFNTARIDHLYVDPHSPNAKLFLHYGDLSDGARLVTLLASIRPD
EVYNLAAQSHVRVSFDEPEHTADTTGAGTIRMLEAVRLSGIETRFYQASSSELYGATPPPQNEETPFYPRSPYAAAKLYS
YWITKNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAAAAIKSGSQDYLYMGNLDAVRDWGYAPEYVEGMWRMLQVD
EPNDYVLATGVGITVRDFLEIAFAHVGLRWEDHVRFDERYLRPTEVDALIGDSTRAREKLGWVPTVHGQTLARLMVDADI
EALQHEGSPWFDKVLLDSWGTAPTQEVSAP

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=57.0175438596491, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=57.5070821529745, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI48994969, Length=245, Percent_Identity=27.3469387755102, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788353, Length=205, Percent_Identity=27.3170731707317, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=340, Percent_Identity=56.4705882352941, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=56.1046511627907, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI133901790, Length=340, Percent_Identity=56.4705882352941, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=56.4705882352941, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI133901788, Length=340, Percent_Identity=56.4705882352941, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI133901786, Length=340, Percent_Identity=56.4705882352941, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24158427, Length=344, Percent_Identity=55.8139534883721, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39460; Mature: 39329

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRALITGITGQDGSYLAELLLHKGYEVHGLIRRASTFNTARIDHLYVDPHSPNAKLFL
CCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEE
HYGDLSDGARLVTLLASIRPDEVYNLAAQSHVRVSFDEPEHTADTTGAGTIRMLEAVRLS
EECCCCCCHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHC
GIETRFYQASSSELYGATPPPQNEETPFYPRSPYAAAKLYSYWITKNYREAYGLFAVNGI
CCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCEEEEEEE
LFNHESPRRGETFVTRKITRAAAAIKSGSQDYLYMGNLDAVRDWGYAPEYVEGMWRMLQV
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCHHHHHHHHHHEEE
DEPNDYVLATGVGITVRDFLEIAFAHVGLRWEDHVRFDERYLRPTEVDALIGDSTRAREK
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHCCCCHHHHEECCCHHHHHH
LGWVPTVHGQTLARLMVDADIEALQHEGSPWFDKVLLDSWGTAPTQEVSAP
HCCCCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKRALITGITGQDGSYLAELLLHKGYEVHGLIRRASTFNTARIDHLYVDPHSPNAKLFL
CCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEE
HYGDLSDGARLVTLLASIRPDEVYNLAAQSHVRVSFDEPEHTADTTGAGTIRMLEAVRLS
EECCCCCCHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHC
GIETRFYQASSSELYGATPPPQNEETPFYPRSPYAAAKLYSYWITKNYREAYGLFAVNGI
CCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCEEEEEEE
LFNHESPRRGETFVTRKITRAAAAIKSGSQDYLYMGNLDAVRDWGYAPEYVEGMWRMLQV
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCHHHHHHHHHHEEE
DEPNDYVLATGVGITVRDFLEIAFAHVGLRWEDHVRFDERYLRPTEVDALIGDSTRAREK
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHCCCCHHHHEECCCHHHHHH
LGWVPTVHGQTLARLMVDADIEALQHEGSPWFDKVLLDSWGTAPTQEVSAP
HCCCCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]