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The map label for this gene is fcl [H]

Identifier: 119867014

GI number: 119867014

Start: 1048301

End: 1049275

Strand: Reverse

Name: fcl [H]

Synonym: Mkms_0962

Alternate gene names: 119867014

Gene position: 1049275-1048301 (Counterclockwise)

Preceding gene: 119867015

Following gene: 119867012

Centisome position: 18.29

GC content: 55.49

Gene sequence:

>975_bases
ATGACCGCTGCGCTTCAATTTCCACCTAACGAATTGGATCGCGGCGCAAACGTTTATGTTGCCGGGCATCGTGGCCTGGT
CGGCTCCGCGATCGTGCGAAGATTGCGCGATGCGGAGTTCAGGAGAATAATAGGCAAGACCTCAGTAGAACTCGACCTGA
AGAATCGCGGTGCCGTCTTTGCATTCTTCAGGGAAGTTAAGCCTAAATATGTCATACTCGCCGCGGCAAAGGTCGGGGGA
ATTTTAGCTAACAGCACTCACCCCGTCGACTTTCTCAGCGAGAACATCCGGATTCAGGTTAATGTGCTTGACGCGGCGAT
TGAAGCCGAGGTGGAGCGCTTGTTGTTTCTCGGTTCCTCTTGCATCTATCCCAAATTTGCCGAACAACCGATCCGCGAGG
AATCGCTGCTTACAGGCCACCTAGAGCCGACCAACGATGCATACGCCATCGCGAAAATTGCTGGCATATTGCATGTTCAA
GCCGTCCGTAGACAATACGGATTGCCTTGGATTTCGGCGATGCCTACTAACCTCTACGGGCCGAACGATAATTTCTCCCT
CACCGGATCGCATGTGCTCCCTGCACTGATCCGACGTTACGACGAGGCCGTCGCATCCGGAGCGCAATCCGTGACTAACT
GGGGTAGCGGCAAGCCACGTCGTGAGTTCCTACACGCCGACGACATGGCAGATGCCTGCTTGCACCTACTTGAACACTAC
GATGGACCAGAACAAGTCAACGTCGGGAGTGGCACCGACGCTACAATCCGTGAAATCGCTGAAACCGTCGCATCAGCAGT
GGGCTACATGGGTGAAACGGCGTGGGATACTTCGAAACCTGACGGCACGCCGCAAAAGCTTCTCGACATCTCAAGGCTCA
CGCGCTCTGGTTGGACCGCAAAAATCGGCTTGGCTGAGGGGATAGAGCGAACCGTGGCGTGGTACCGCCGGAACGTTGGC
GGGTTGCGCGGGTAA

Upstream 100 bases:

>100_bases
ACGCCGACATTGAAGCGCTCCAGCATGAGGGGAGCCCTTGGTTCGACAAGGTGCTCTTGGATTCGTGGGGGACTGCGCCC
ACTCAAGAGGTGAGTGCGCC

Downstream 100 bases:

>100_bases
TGAGTCGAAGCCGTCTATACCTCAGCTTCTGATTGCCCCCCCTCTGCCTGTTCGGGCCGCGCACCGGCAATCGTTGTTTC
GACGAGACTCTCCCACTGCG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTAALQFPPNELDRGANVYVAGHRGLVGSAIVRRLRDAEFRRIIGKTSVELDLKNRGAVFAFFREVKPKYVILAAAKVGG
ILANSTHPVDFLSENIRIQVNVLDAAIEAEVERLLFLGSSCIYPKFAEQPIREESLLTGHLEPTNDAYAIAKIAGILHVQ
AVRRQYGLPWISAMPTNLYGPNDNFSLTGSHVLPALIRRYDEAVASGAQSVTNWGSGKPRREFLHADDMADACLHLLEHY
DGPEQVNVGSGTDATIREIAETVASAVGYMGETAWDTSKPDGTPQKLLDISRLTRSGWTAKIGLAEGIERTVAWYRRNVG
GLRG

Sequences:

>Translated_324_residues
MTAALQFPPNELDRGANVYVAGHRGLVGSAIVRRLRDAEFRRIIGKTSVELDLKNRGAVFAFFREVKPKYVILAAAKVGG
ILANSTHPVDFLSENIRIQVNVLDAAIEAEVERLLFLGSSCIYPKFAEQPIREESLLTGHLEPTNDAYAIAKIAGILHVQ
AVRRQYGLPWISAMPTNLYGPNDNFSLTGSHVLPALIRRYDEAVASGAQSVTNWGSGKPRREFLHADDMADACLHLLEHY
DGPEQVNVGSGTDATIREIAETVASAVGYMGETAWDTSKPDGTPQKLLDISRLTRSGWTAKIGLAEGIERTVAWYRRNVG
GLRG
>Mature_323_residues
TAALQFPPNELDRGANVYVAGHRGLVGSAIVRRLRDAEFRRIIGKTSVELDLKNRGAVFAFFREVKPKYVILAAAKVGGI
LANSTHPVDFLSENIRIQVNVLDAAIEAEVERLLFLGSSCIYPKFAEQPIREESLLTGHLEPTNDAYAIAKIAGILHVQA
VRRQYGLPWISAMPTNLYGPNDNFSLTGSHVLPALIRRYDEAVASGAQSVTNWGSGKPRREFLHADDMADACLHLLEHYD
GPEQVNVGSGTDATIREIAETVASAVGYMGETAWDTSKPDGTPQKLLDISRLTRSGWTAKIGLAEGIERTVAWYRRNVGG
LRG

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=307, Percent_Identity=32.5732899022801, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI7657641, Length=230, Percent_Identity=23.9130434782609, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788365, Length=316, Percent_Identity=51.8987341772152, Blast_Score=324, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17554428, Length=309, Percent_Identity=31.3915857605178, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI115532424, Length=333, Percent_Identity=23.4234234234234, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922778, Length=318, Percent_Identity=33.0188679245283, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35435; Mature: 35303

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAALQFPPNELDRGANVYVAGHRGLVGSAIVRRLRDAEFRRIIGKTSVELDLKNRGAVF
CCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHH
AFFREVKPKYVILAAAKVGGILANSTHPVDFLSENIRIQVNVLDAAIEAEVERLLFLGSS
HHHHHCCCCEEEEEEHHHCCEECCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCC
CIYPKFAEQPIREESLLTGHLEPTNDAYAIAKIAGILHVQAVRRQYGLPWISAMPTNLYG
CCCCHHHCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCC
PNDNFSLTGSHVLPALIRRYDEAVASGAQSVTNWGSGKPRREFLHADDMADACLHLLEHY
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHC
DGPEQVNVGSGTDATIREIAETVASAVGYMGETAWDTSKPDGTPQKLLDISRLTRSGWTA
CCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
KIGLAEGIERTVAWYRRNVGGLRG
EEHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TAALQFPPNELDRGANVYVAGHRGLVGSAIVRRLRDAEFRRIIGKTSVELDLKNRGAVF
CCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHH
AFFREVKPKYVILAAAKVGGILANSTHPVDFLSENIRIQVNVLDAAIEAEVERLLFLGSS
HHHHHCCCCEEEEEEHHHCCEECCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCC
CIYPKFAEQPIREESLLTGHLEPTNDAYAIAKIAGILHVQAVRRQYGLPWISAMPTNLYG
CCCCHHHCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCC
PNDNFSLTGSHVLPALIRRYDEAVASGAQSVTNWGSGKPRREFLHADDMADACLHLLEHY
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHC
DGPEQVNVGSGTDATIREIAETVASAVGYMGETAWDTSKPDGTPQKLLDISRLTRSGWTA
CCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
KIGLAEGIERTVAWYRRNVGGLRG
EEHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]