Click here to switch to the map view.

The map label for this gene is potE [H]

Identifier: 119776467

GI number: 119776467

Start: 3962848

End: 3964164

Strand: Direct

Name: potE [H]

Synonym: Sama_3335

Alternate gene names: 119776467

Gene position: 3962848-3964164 (Clockwise)

Preceding gene: 119776466

Following gene: 119776468

Centisome position: 92.03

GC content: 57.63

Gene sequence:

>1317_bases
ATGAGTAAATCAAACAACAAGATAGGCGTGGTTCAACTCACCATTCTCACCATAGTTAACATGATGGGCTCAGGTATCAT
CATGCTGCCGACCCAGCTGGCACAGGTGGGGACCATTTCGATATTGTCCTGGCTGGTTACGGCCGCAGGCTCAACGGCGC
TGGCCTTCGCTTTTGCCAAGTGCGGCATGTTCAGTAAAAAGTCCGGCGGCATGGGCGGCTACGCCGAATACGCCTTCGGC
CGCAGCGGTAACTTTATGGCCAACTACACCTATGCGGTGTCGTTGCTGATTGCCAACGTGGCCATTGCCATCTCGGCGGT
GGGCTATGGTGCCGTGCTCTTTGACGTGGAGCTGAGCCCCATCGCCACCTGTCTGGCCACCATAGGTGTACTGTGGCTCG
CCACGGTCGCCAACTTTGGGGGTGCCCGCATCACGGGTCAGGTGTCCAGCATCACTGTGTGGGGCATCATTCTGCCGGTG
ATTGGGGTGTCGCTTATCGGTTGGTTCTGGTTTGACTTTGACCTCTACAAGTCAGCCTGGAATCCACACGATCTGCCCTT
CTTCGAAGCGCTGGGCGGCTCCATCGCCATGACCCTGTGGGCCTTCCTGGGCCTGGAATCTGCCTGTGCCAACTCAGAAG
CGGTTGAAAACCCAGAGAAGAACGTTCCTATCGCGGTTATGGGCGGCACCCTGGGCGCGGCACTGATTTATATCGTGTCC
ACCAACGTGATTGCCGGTATTGTGCCAAACGGTGAGCTGGCGCTGTCCAACGCGCCTTTCGGTCTGGCCTTTGCCCAGAT
GTTCAACCCCACCATTGGTGCCATCGTCATGGCCTGCGCCATCATCAGCTGTACCGGCTCGCTGCTGGGCTGGCAGTTCA
CCATTGCACAGGTGTTCAAGGCCTCTGCTGACGAAGGCTTCTTCCCCAAACTCTTCTCCAAGGTGACCAAGGCCGACGCC
CCCGTGCTGGGCATGCTGGCGATAGTTTCAATCCAGTCTGTGCTAAGCCTGATGACCATCAGCCCCTCGCTGAACAAGCA
ATTTGAAGCCCTGGTAAACCTGGCAGTGGTGACCAACATCATCCCTTACATCCTGTCTATGGCCGCCCTCGGCGTGATGC
AAAAGCAGATGAAGATCCCGGTCAGCAAGGCCCGCATCGCCAACTGCATGGCAGTAATGGGCGCGCTCTACAGCTTCTAT
GCCCTGTACAGCTCGGGCGAGACCGCCGTGATGCTGGGCTCCATTGCCACCTTCTTCGGCTGGACCCTGTATGGCGTGAT
CTCCAAGAAAAACGAACTGCGCCTGCAACAGGCCTGA

Upstream 100 bases:

>100_bases
TGGCCGGGTTCAGGCTATGGGTTACGTCCTCAAACGCTGATCAACGCGGGGCGGCAAGGGTACGCCGCCCCCTAAAACCA
TAACGAATCGAGACACTATT

Downstream 100 bases:

>100_bases
ACACAACACACATAAGGCCTCGTCTTGTGGCGAGGCCACAACAAAGAAATTCAAAGGTGGAATCATGAAAAAATCACAAT
TGGCACTCTTACTGCCACTG

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSKSNNKIGVVQLTILTIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAFAFAKCGMFSKKSGGMGGYAEYAFG
RSGNFMANYTYAVSLLIANVAIAISAVGYGAVLFDVELSPIATCLATIGVLWLATVANFGGARITGQVSSITVWGIILPV
IGVSLIGWFWFDFDLYKSAWNPHDLPFFEALGGSIAMTLWAFLGLESACANSEAVENPEKNVPIAVMGGTLGAALIYIVS
TNVIAGIVPNGELALSNAPFGLAFAQMFNPTIGAIVMACAIISCTGSLLGWQFTIAQVFKASADEGFFPKLFSKVTKADA
PVLGMLAIVSIQSVLSLMTISPSLNKQFEALVNLAVVTNIIPYILSMAALGVMQKQMKIPVSKARIANCMAVMGALYSFY
ALYSSGETAVMLGSIATFFGWTLYGVISKKNELRLQQA

Sequences:

>Translated_438_residues
MSKSNNKIGVVQLTILTIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAFAFAKCGMFSKKSGGMGGYAEYAFG
RSGNFMANYTYAVSLLIANVAIAISAVGYGAVLFDVELSPIATCLATIGVLWLATVANFGGARITGQVSSITVWGIILPV
IGVSLIGWFWFDFDLYKSAWNPHDLPFFEALGGSIAMTLWAFLGLESACANSEAVENPEKNVPIAVMGGTLGAALIYIVS
TNVIAGIVPNGELALSNAPFGLAFAQMFNPTIGAIVMACAIISCTGSLLGWQFTIAQVFKASADEGFFPKLFSKVTKADA
PVLGMLAIVSIQSVLSLMTISPSLNKQFEALVNLAVVTNIIPYILSMAALGVMQKQMKIPVSKARIANCMAVMGALYSFY
ALYSSGETAVMLGSIATFFGWTLYGVISKKNELRLQQA
>Mature_437_residues
SKSNNKIGVVQLTILTIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAFAFAKCGMFSKKSGGMGGYAEYAFGR
SGNFMANYTYAVSLLIANVAIAISAVGYGAVLFDVELSPIATCLATIGVLWLATVANFGGARITGQVSSITVWGIILPVI
GVSLIGWFWFDFDLYKSAWNPHDLPFFEALGGSIAMTLWAFLGLESACANSEAVENPEKNVPIAVMGGTLGAALIYIVST
NVIAGIVPNGELALSNAPFGLAFAQMFNPTIGAIVMACAIISCTGSLLGWQFTIAQVFKASADEGFFPKLFSKVTKADAP
VLGMLAIVSIQSVLSLMTISPSLNKQFEALVNLAVVTNIIPYILSMAALGVMQKQMKIPVSKARIANCMAVMGALYSFYA
LYSSGETAVMLGSIATFFGWTLYGVISKKNELRLQQA

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=433, Percent_Identity=75.5196304849885, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=407, Percent_Identity=35.3808353808354, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790575, Length=376, Percent_Identity=32.7127659574468, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1787890, Length=422, Percent_Identity=25.8293838862559, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46142; Mature: 46011

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSNNKIGVVQLTILTIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAFAFAK
CCCCCCCEEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
CGMFSKKSGGMGGYAEYAFGRSGNFMANYTYAVSLLIANVAIAISAVGYGAVLFDVELSP
CCCCCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHH
IATCLATIGVLWLATVANFGGARITGQVSSITVWGIILPVIGVSLIGWFWFDFDLYKSAW
HHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NPHDLPFFEALGGSIAMTLWAFLGLESACANSEAVENPEKNVPIAVMGGTLGAALIYIVS
CCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEECCHHHHHHHHHHH
TNVIAGIVPNGELALSNAPFGLAFAQMFNPTIGAIVMACAIISCTGSLLGWQFTIAQVFK
HHHHEEECCCCCEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASADEGFFPKLFSKVTKADAPVLGMLAIVSIQSVLSLMTISPSLNKQFEALVNLAVVTNI
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IPYILSMAALGVMQKQMKIPVSKARIANCMAVMGALYSFYALYSSGETAVMLGSIATFFG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
WTLYGVISKKNELRLQQA
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKSNNKIGVVQLTILTIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAFAFAK
CCCCCCEEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
CGMFSKKSGGMGGYAEYAFGRSGNFMANYTYAVSLLIANVAIAISAVGYGAVLFDVELSP
CCCCCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHH
IATCLATIGVLWLATVANFGGARITGQVSSITVWGIILPVIGVSLIGWFWFDFDLYKSAW
HHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NPHDLPFFEALGGSIAMTLWAFLGLESACANSEAVENPEKNVPIAVMGGTLGAALIYIVS
CCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEECCHHHHHHHHHHH
TNVIAGIVPNGELALSNAPFGLAFAQMFNPTIGAIVMACAIISCTGSLLGWQFTIAQVFK
HHHHEEECCCCCEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASADEGFFPKLFSKVTKADAPVLGMLAIVSIQSVLSLMTISPSLNKQFEALVNLAVVTNI
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IPYILSMAALGVMQKQMKIPVSKARIANCMAVMGALYSFYALYSSGETAVMLGSIATFFG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
WTLYGVISKKNELRLQQA
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]