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The map label for this gene is hemX [H]

Identifier: 119776388

GI number: 119776388

Start: 3850549

End: 3851739

Strand: Direct

Name: hemX [H]

Synonym: Sama_3256

Alternate gene names: 119776388

Gene position: 3850549-3851739 (Clockwise)

Preceding gene: 119776387

Following gene: 119776389

Centisome position: 89.42

GC content: 54.66

Gene sequence:

>1191_bases
ATGGAAAACCAACAGCAAGAGGCCGCCGCAGCAGAGTCCACTACTTTGCCTGCCACACCGTCTTCTCAACCTAATGACAT
AGCCCCCAAAAAAGAACCCCGTCATGGCAGCTCGTTGGCCATACGAGTGGGAGTATTGGTGGCATTGCTTATGGGGGGAA
GTGCCCTTGGCGGTGGCTACTACCTCTATCAACAGTTGGTGGAGCAGTACCACTACACACTGTCGGTGGAGGAGAAACAG
AGTGCCTCGCTGAAAGAGCCGGTGTCACGCCTGAATCAATTGGAACAAAGGTTAAAGCAGATTGGCCAATTGGAGACTGA
GCTTGGGCGCCTTACCGCCGATCAGAGTGCCCTCGAGCGTCGCCTGACCAACCTGTCTCAAAAAACGCCCGACGATTGGC
TGATTGCCGAAGCTGACTATCTGGTGCGCATGGCAGGCCGTAAACTGTGGCTTGAGCAAGATCCCGCCACTGCGCTGAGC
TTGCTGAAAACAGCCGATGAGCGGATCGCCGCCATGCAGCAGCCTTCACTGTTCAGCCTGCGCAAAGCACTGGCGGCCGA
TATGGAACAGGTGGCCTCTGTACGCAGTACCGATATTGCTGGCAACGTTTATGCGTTAGACGAAATCATGAAACGTATCG
AGCGATTGACCCCGGGCCGTAATAAGCAGGAATACACACCACCGACCGATGAGACCATCAGCGACTCTCTGAATGACTGG
CAAACCAACCTGGCAAAGAGTTGGAAAGCGCTGATTAATGACTTTGTGACCGTACGTCAACGCACCGGCGATATCACGCC
GCTCCTTTCACCGGAACAAGAGTGGTATCTGAAAGAAAACATTCGCACCAAGCTGCTCCAGGCCCAGCTTGCGCTGTATA
GACACGATGAACTGAGCTTCCGGGAAGCCATTTTAATGGCAGGTGGTTGGGTAAAACTTTACTTCAGTGTGGACTCAGGT
ACCGGGGCGCAAATACTTGAGCGTCTCGACTCTCTGGCGTCCCTGCGTATCGATGCAGTACCCATCAGTAAATTCCAGTC
CAGCGATATGCTCAGAACCCTGGTTCAGGAAGGCGTCAAGGCACCTCAGCCAATCCCCGATCCCACGCCAATACCGGCAG
TGAATCCGCTGCCCGAACCGGATCAGGAACCAGAACCTTCTGCGGCAGCCGAATCGGAGGCTGAGGCATGA

Upstream 100 bases:

>100_bases
AGCAGCAAACCAAGCCGCCGTACTGGACGCATTGGGCATGTAAACTTTTGCTGCTTTAGTTTCCCTGAATTGCACGCTTT
CTAGCATCAAGGATTGTTTC

Downstream 100 bases:

>100_bases
TAAAAACGCTCATTTATCTTGGCATTATTCTGATTGGGCTCTGCTTGAGCCCCTACATTGTTGGCAACACCGGCTATCTG
TATTTGTCAGCCTGGGGCTA

Product: hemX protein

Products: NA

Alternate protein names: Urogen-III methylase; ORF X [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MENQQQEAAAAESTTLPATPSSQPNDIAPKKEPRHGSSLAIRVGVLVALLMGGSALGGGYYLYQQLVEQYHYTLSVEEKQ
SASLKEPVSRLNQLEQRLKQIGQLETELGRLTADQSALERRLTNLSQKTPDDWLIAEADYLVRMAGRKLWLEQDPATALS
LLKTADERIAAMQQPSLFSLRKALAADMEQVASVRSTDIAGNVYALDEIMKRIERLTPGRNKQEYTPPTDETISDSLNDW
QTNLAKSWKALINDFVTVRQRTGDITPLLSPEQEWYLKENIRTKLLQAQLALYRHDELSFREAILMAGGWVKLYFSVDSG
TGAQILERLDSLASLRIDAVPISKFQSSDMLRTLVQEGVKAPQPIPDPTPIPAVNPLPEPDQEPEPSAAAESEAEA

Sequences:

>Translated_396_residues
MENQQQEAAAAESTTLPATPSSQPNDIAPKKEPRHGSSLAIRVGVLVALLMGGSALGGGYYLYQQLVEQYHYTLSVEEKQ
SASLKEPVSRLNQLEQRLKQIGQLETELGRLTADQSALERRLTNLSQKTPDDWLIAEADYLVRMAGRKLWLEQDPATALS
LLKTADERIAAMQQPSLFSLRKALAADMEQVASVRSTDIAGNVYALDEIMKRIERLTPGRNKQEYTPPTDETISDSLNDW
QTNLAKSWKALINDFVTVRQRTGDITPLLSPEQEWYLKENIRTKLLQAQLALYRHDELSFREAILMAGGWVKLYFSVDSG
TGAQILERLDSLASLRIDAVPISKFQSSDMLRTLVQEGVKAPQPIPDPTPIPAVNPLPEPDQEPEPSAAAESEAEA
>Mature_396_residues
MENQQQEAAAAESTTLPATPSSQPNDIAPKKEPRHGSSLAIRVGVLVALLMGGSALGGGYYLYQQLVEQYHYTLSVEEKQ
SASLKEPVSRLNQLEQRLKQIGQLETELGRLTADQSALERRLTNLSQKTPDDWLIAEADYLVRMAGRKLWLEQDPATALS
LLKTADERIAAMQQPSLFSLRKALAADMEQVASVRSTDIAGNVYALDEIMKRIERLTPGRNKQEYTPPTDETISDSLNDW
QTNLAKSWKALINDFVTVRQRTGDITPLLSPEQEWYLKENIRTKLLQAQLALYRHDELSFREAILMAGGWVKLYFSVDSG
TGAQILERLDSLASLRIDAVPISKFQSSDMLRTLVQEGVKAPQPIPDPTPIPAVNPLPEPDQEPEPSAAAESEAEA

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790235, Length=402, Percent_Identity=31.8407960199005, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007470 [H]

Pfam domain/function: PF04375 HemX [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 43945; Mature: 43945

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQQQEAAAAESTTLPATPSSQPNDIAPKKEPRHGSSLAIRVGVLVALLMGGSALGGGY
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
YLYQQLVEQYHYTLSVEEKQSASLKEPVSRLNQLEQRLKQIGQLETELGRLTADQSALER
HHHHHHHHHHHCEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RLTNLSQKTPDDWLIAEADYLVRMAGRKLWLEQDPATALSLLKTADERIAAMQQPSLFSL
HHHHHHHCCCCCCEEEHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
RKALAADMEQVASVRSTDIAGNVYALDEIMKRIERLTPGRNKQEYTPPTDETISDSLNDW
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
QTNLAKSWKALINDFVTVRQRTGDITPLLSPEQEWYLKENIRTKLLQAQLALYRHDELSF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
REAILMAGGWVKLYFSVDSGTGAQILERLDSLASLRIDAVPISKFQSSDMLRTLVQEGVK
HHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
APQPIPDPTPIPAVNPLPEPDQEPEPSAAAESEAEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MENQQQEAAAAESTTLPATPSSQPNDIAPKKEPRHGSSLAIRVGVLVALLMGGSALGGGY
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
YLYQQLVEQYHYTLSVEEKQSASLKEPVSRLNQLEQRLKQIGQLETELGRLTADQSALER
HHHHHHHHHHHCEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RLTNLSQKTPDDWLIAEADYLVRMAGRKLWLEQDPATALSLLKTADERIAAMQQPSLFSL
HHHHHHHCCCCCCEEEHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
RKALAADMEQVASVRSTDIAGNVYALDEIMKRIERLTPGRNKQEYTPPTDETISDSLNDW
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
QTNLAKSWKALINDFVTVRQRTGDITPLLSPEQEWYLKENIRTKLLQAQLALYRHDELSF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
REAILMAGGWVKLYFSVDSGTGAQILERLDSLASLRIDAVPISKFQSSDMLRTLVQEGVK
HHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
APQPIPDPTPIPAVNPLPEPDQEPEPSAAAESEAEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3062586; 3054815; 1379743; 9278503; 9298646 [H]