Click here to switch to the map view.

The map label for this gene is pgtA [H]

Identifier: 119776375

GI number: 119776375

Start: 3838657

End: 3839946

Strand: Direct

Name: pgtA [H]

Synonym: Sama_3243

Alternate gene names: 119776375

Gene position: 3838657-3839946 (Clockwise)

Preceding gene: 119776374

Following gene: 119776376

Centisome position: 89.14

GC content: 57.21

Gene sequence:

>1290_bases
ATGTCCCATCTGCTTAAACCCAAGCCTAATCTGCTGCTTATAGACGATGATGAAGACGTACTGGACGCTTGCCGCCAGTT
GTTTAACTTATCCGGCTATCAGGTGCGGGCAACCAACTCACCGCAGAAGGCACTGGAGGCATTGAGCCGCGACTGGGACG
GAGTGGTCATCAGCGACATCTATATGCCCGCCATGCATGGCCTGGATTTGCTGGCAGCCGTGCACCATATTGACCCACAG
ATCCCTGTCATCATGATCACGGGGCACGCCGATATTCCTCTGGCGGTGAAGGCCGTAAAACAGGGCGCCGCAGACTTTAT
CGAAAAGCCCCTCGACCCGCCGACCTTTCTTGCCCTGGTAAAAAAGGCCGCCACCCAACGCCATCAATTGATTGGCCAGC
GGCAGAACATTCAGGACTCTCTGGCCGGTCAGCTCCTTGGCAGCAGCGCCCAAATCAATGATCTGCGGGAACAGCTCGGG
CAACTGGCCTTAACCGACAGAGACGTGATGCTGGAGGGGCCTCGGGGGGTTGGCCGAAATACCCTGGCGGCCTTGCTGCA
CAAACTGGGCCCCAGGGCAGAAGGGCCACTGCAACAGCTCGAATGCGCTCTGGTGGAAAGTCCGGAAAAACTGCAACAGG
CGGTGGACGCGAGCCGCGGCGGCACCCTGATCCTGAGGGAGCTTGAGCATCTACCCACCGACAGCCAGCGCTGGCTGGCA
AGCTTTCTCCTCGATCAGGAACGCAGGGGCAACAAAGAAGTGCGCACCATCGCCATTCTGGAAAGTCAGCCCGAAACCCT
GGTACAGGAGCAAACCCTCACCCCCGAGTGCTTTTATTATTTCTCCCAGGTCAGGCTGCGTCTGCCGGCCCTGTGCGAAC
GACCAAGCGATATAGTGCCGCTGTTTATGGCCTTTTTGAAAGAAAGCTGCCGCCGCCTGTCCCGTAAGATGCCCACGGTG
GAAAATCGCTACCTGGACAATCTCAAGCAGCACAGCTGGCCCGGCAACGTGCGGGAACTTCGCAACGTGGCCGAGCTCTA
TGCGGTGGGGATAGTCAAACTGGCCAATGTGCAGCGAATTGCGGCGGCGCCCTCTGTAAAAGGCCCATTGGATGAGCTGG
TGGACGACTATGAAAAGCGCCTGATAGAAGATGCGTTGTTTCTGTTCTCTGGCCGGGTATCAGAGGCGGCAGACTACCTT
AACGTGCCCCGTAAGAAATTGTATTTAAGGATGAAAAAGTACGGCCTCGATAAGGAATGTTTTAAGCCGGTGAAGCCGGG
GCGGGGCTAA

Upstream 100 bases:

>100_bases
GGACTTTCGATTTGCCGAACCCTGATGGAACGCCAGGAAGGCGATATCCGCCTTGCCTGCGCTATGGACGGCAACACCCT
GATGCTGTTGGAGTTTACAA

Downstream 100 bases:

>100_bases
GGGTCACTGGCCATGGGGCAAAACCCGTGTTATATCAGGGAAAAGAGCAATTCAAGGACAGCCCATGACCCAATTTGCCC
TCGATGATATTCAGCATCTG

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSHLLKPKPNLLLIDDDEDVLDACRQLFNLSGYQVRATNSPQKALEALSRDWDGVVISDIYMPAMHGLDLLAAVHHIDPQ
IPVIMITGHADIPLAVKAVKQGAADFIEKPLDPPTFLALVKKAATQRHQLIGQRQNIQDSLAGQLLGSSAQINDLREQLG
QLALTDRDVMLEGPRGVGRNTLAALLHKLGPRAEGPLQQLECALVESPEKLQQAVDASRGGTLILRELEHLPTDSQRWLA
SFLLDQERRGNKEVRTIAILESQPETLVQEQTLTPECFYYFSQVRLRLPALCERPSDIVPLFMAFLKESCRRLSRKMPTV
ENRYLDNLKQHSWPGNVRELRNVAELYAVGIVKLANVQRIAAAPSVKGPLDELVDDYEKRLIEDALFLFSGRVSEAADYL
NVPRKKLYLRMKKYGLDKECFKPVKPGRG

Sequences:

>Translated_429_residues
MSHLLKPKPNLLLIDDDEDVLDACRQLFNLSGYQVRATNSPQKALEALSRDWDGVVISDIYMPAMHGLDLLAAVHHIDPQ
IPVIMITGHADIPLAVKAVKQGAADFIEKPLDPPTFLALVKKAATQRHQLIGQRQNIQDSLAGQLLGSSAQINDLREQLG
QLALTDRDVMLEGPRGVGRNTLAALLHKLGPRAEGPLQQLECALVESPEKLQQAVDASRGGTLILRELEHLPTDSQRWLA
SFLLDQERRGNKEVRTIAILESQPETLVQEQTLTPECFYYFSQVRLRLPALCERPSDIVPLFMAFLKESCRRLSRKMPTV
ENRYLDNLKQHSWPGNVRELRNVAELYAVGIVKLANVQRIAAAPSVKGPLDELVDDYEKRLIEDALFLFSGRVSEAADYL
NVPRKKLYLRMKKYGLDKECFKPVKPGRG
>Mature_428_residues
SHLLKPKPNLLLIDDDEDVLDACRQLFNLSGYQVRATNSPQKALEALSRDWDGVVISDIYMPAMHGLDLLAAVHHIDPQI
PVIMITGHADIPLAVKAVKQGAADFIEKPLDPPTFLALVKKAATQRHQLIGQRQNIQDSLAGQLLGSSAQINDLREQLGQ
LALTDRDVMLEGPRGVGRNTLAALLHKLGPRAEGPLQQLECALVESPEKLQQAVDASRGGTLILRELEHLPTDSQRWLAS
FLLDQERRGNKEVRTIAILESQPETLVQEQTLTPECFYYFSQVRLRLPALCERPSDIVPLFMAFLKESCRRLSRKMPTVE
NRYLDNLKQHSWPGNVRELRNVAELYAVGIVKLANVQRIAAAPSVKGPLDELVDDYEKRLIEDALFLFSGRVSEAADYLN
VPRKKLYLRMKKYGLDKECFKPVKPGRG

Specific function: Member of the two-component regulatory system pgtB/pgtA that regulates the inducible phosphoglycerate transport system. When activated by pgtB it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=27.4336283185841, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1790299, Length=465, Percent_Identity=28.3870967741935, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788905, Length=454, Percent_Identity=27.5330396475771, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790437, Length=436, Percent_Identity=27.5229357798165, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI87082117, Length=245, Percent_Identity=30.6122448979592, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=29.483282674772, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=25.2307692307692, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=27.8637770897833, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081872, Length=269, Percent_Identity=28.2527881040892, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=24.6105919003115, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081858, Length=295, Percent_Identity=24.406779661017, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=26.0061919504644, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48104; Mature: 47973

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLLKPKPNLLLIDDDEDVLDACRQLFNLSGYQVRATNSPQKALEALSRDWDGVVISDI
CCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEHHHH
YMPAMHGLDLLAAVHHIDPQIPVIMITGHADIPLAVKAVKQGAADFIEKPLDPPTFLALV
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KKAATQRHQLIGQRQNIQDSLAGQLLGSSAQINDLREQLGQLALTDRDVMLEGPRGVGRN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHH
TLAALLHKLGPRAEGPLQQLECALVESPEKLQQAVDASRGGTLILRELEHLPTDSQRWLA
HHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHH
SFLLDQERRGNKEVRTIAILESQPETLVQEQTLTPECFYYFSQVRLRLPALCERPSDIVP
HHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
LFMAFLKESCRRLSRKMPTVENRYLDNLKQHSWPGNVRELRNVAELYAVGIVKLANVQRI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAAPSVKGPLDELVDDYEKRLIEDALFLFSGRVSEAADYLNVPRKKLYLRMKKYGLDKEC
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHH
FKPVKPGRG
HCCCCCCCC
>Mature Secondary Structure 
SHLLKPKPNLLLIDDDEDVLDACRQLFNLSGYQVRATNSPQKALEALSRDWDGVVISDI
CCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEHHHH
YMPAMHGLDLLAAVHHIDPQIPVIMITGHADIPLAVKAVKQGAADFIEKPLDPPTFLALV
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KKAATQRHQLIGQRQNIQDSLAGQLLGSSAQINDLREQLGQLALTDRDVMLEGPRGVGRN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHH
TLAALLHKLGPRAEGPLQQLECALVESPEKLQQAVDASRGGTLILRELEHLPTDSQRWLA
HHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHH
SFLLDQERRGNKEVRTIAILESQPETLVQEQTLTPECFYYFSQVRLRLPALCERPSDIVP
HHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
LFMAFLKESCRRLSRKMPTVENRYLDNLKQHSWPGNVRELRNVAELYAVGIVKLANVQRI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAAPSVKGPLDELVDDYEKRLIEDALFLFSGRVSEAADYLNVPRKKLYLRMKKYGLDKEC
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHH
FKPVKPGRG
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3023201; 11677609; 2842311 [H]