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The map label for this gene is pifA [H]

Identifier: 119776054

GI number: 119776054

Start: 3474807

End: 3476954

Strand: Reverse

Name: pifA [H]

Synonym: Sama_2922

Alternate gene names: 119776054

Gene position: 3476954-3474807 (Counterclockwise)

Preceding gene: 119776055

Following gene: 119776053

Centisome position: 80.74

GC content: 39.2

Gene sequence:

>2148_bases
ATGGGAAGCTATCATAATGACCAGCCTATTACTGGAGAGTCGGAAAGTCCTGACCTATTAAGAAGAGAGGCTTTTTCTGA
ACATTTAGCTACTGTTCTTCGTATCGCTCCTAGTGATGATTGCATGACGGCCTCTCTAGAAGGGGAATGGGGTTATGGAA
AGACTTCAGTTATAAATCTCGTTAAGAAATCATTAGGCAACCAAGAAAATGGTCCTGTGATAGTTGAGTACAATCCATGG
CTTGCTGGTAAAGCAGAGGCATTAGTACAAGATTTTTTGGTCCAGTTTTCATCCCAGCTTAATATACCTGACAGACCTAA
AGAAGGGCTAAAAGCCGCTAAGGAGCTTTTGGCATATTCGGAATTGTTTAACGCCATGAAATTCATTCCGGGGGTGGAGC
CTTGGGCATCTACAGTTCAAGGTGTTTTCAAAGTGGTTGGTGGAGCCACTAAAAAAATTAGCAAGCTAAAAGAGTTAGAT
TTAATTGGCAGAAAAAATAAGATTAACGATCTTCTAAAGAAGCTTGGGGTATCAATTGTCGTCATAATTGACGATATTGA
TCGCCTGACCCCAGATGAGGCGTTTCAAGTGGTTAGGTTGGTAAAAGCTGTAGCTGACTTTCCTGGGACATCTTTCTTGC
TGTCTTTTGATCCTACTTATCTCGCAAGCTCACTAGAAAAGCACGGCATTGAAAATTCGAACCAATATATAGATAAAGTT
GTTCAGTTACGGATTTCATTGCCTCTTATTGCTCACAATGATCTTCAAAAGCTGGCTGATATAGAACTTCAGAATTTATC
AGATAAATCGTTAACGGATAGCTTTGAACGTGACCAAGAGCGACTTAGCTATCTATATCATAGTTATGTTAAATATTTAA
TACGAAATCCGCGAGAGCTAAAACGAACCTTTAATCATTTACGTTTCGTGCTCTCACAAACAGAAGGCAACGTATGTTTT
ACTGATTTATACTGCTTGTCTGTCTTGGCTATCAAAGCTCAAGAAATATACCATTCCCTAAAAGAGTCTCCTGAATATTA
TGTAGGTCGAAAATTCGATGACAGTCTTGCTTTTGATAAGAGAGAAGAGGTTGTAGAGAAAAACAAAGATAAACGAGATA
GTATTATTACGCGCGCACCTGGAAAAGATGTGGTTTATCTAAAAGGCATTCTCAAAGAATTATTTCCACTTCTCGAAGGT
TCCGGTTATTCGATGTATGGTTCCGATTATGATCAGTGTGGACGGGTTGCATCAGAGAAAAGACTTTATATCGCACTTCA
TTATCAGATTCCAACGGGATTCGCATCAGATACCGACATAGTTTCATTTTTAAATGGATCAATCAATCGCGAGGAGTATT
TGAGAAGAGCGATTTCTGAAGGTTTCGTAGAGCGATTATTTGAACTTTTGCACCATAATATTGAGAAGGCTAACCAAGAG
AATGCTGCTCTCTCATTAAAGGCTCTATATACGGTCTTTTTAGATTCGCCGTACTTAAAGAGCTTTGAAGATGCTGTTCA
CGGTTTCTTTGGGTTTGAACCATATCGAAATATAATGTGGTTGACATTTGCTTTTATTAAGGTCTTGGATGAAAAGGAGC
AATTTCTCTTCGATTTGCTCCAAAATCCCAGCTTTCTACCAATTACAGCTGACATAAGTCGTCGACTATTAGTGCAGCTA
GGAAGGATAGAGACAGATGATCCGAGATTAAAAGAAGAGCAGTGGCTATCCCCCGAAAAAGGAGAAGAATACCTTAAAAC
TTGGTCCGACATGGTTGCGAGTCAGTTACTAGATGGTGGGCTTGCCGACTCTATTCATGCATCACATATTTATTTTGTTT
TCTATCGAGTGTCTAAGGAAAAAACAAGGCAAGTTTTCAGTAAGTGGATAAATGAAGAGCTAGGCATAGAAAAGATAGCT
AAGCTCGTGGGTCGATGCGGGAGTGATTCTACTAACGGACCATACACAGAAATATCTGAGGATTCATTATCTGAACTATT
GGATTATCAGCGTCTTAAGGAGCTTGCTGACGTGGAATTAAGCAATACCAGCCAAACGCTTTCGAATTACTTGAAAGCCG
TTTACTCAAGCGTTCTGACTGGTGAAAAATATTATCTTAATGATGCTACAAAAGGCGAAAAATTCTAA

Upstream 100 bases:

>100_bases
TAAGCTGATAAACGAGCCGGCACACTATACAAATGTTATGTCTCTTGAGGTCATTTCGGTACTTCAAGTTATGAAAGAGT
AGAAAGGAAGAAATAAGTAC

Downstream 100 bases:

>100_bases
AATATTCTGCAAAGATGGGAGACATAACAAGCGGCTCCAGCGGATTCACTTCGCTCACCGCTGAGCCGGGCGTTATCTCC
GTGAAAGCCTTAGCTTGTGC

Product: P-loop ATPase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MGSYHNDQPITGESESPDLLRREAFSEHLATVLRIAPSDDCMTASLEGEWGYGKTSVINLVKKSLGNQENGPVIVEYNPW
LAGKAEALVQDFLVQFSSQLNIPDRPKEGLKAAKELLAYSELFNAMKFIPGVEPWASTVQGVFKVVGGATKKISKLKELD
LIGRKNKINDLLKKLGVSIVVIIDDIDRLTPDEAFQVVRLVKAVADFPGTSFLLSFDPTYLASSLEKHGIENSNQYIDKV
VQLRISLPLIAHNDLQKLADIELQNLSDKSLTDSFERDQERLSYLYHSYVKYLIRNPRELKRTFNHLRFVLSQTEGNVCF
TDLYCLSVLAIKAQEIYHSLKESPEYYVGRKFDDSLAFDKREEVVEKNKDKRDSIITRAPGKDVVYLKGILKELFPLLEG
SGYSMYGSDYDQCGRVASEKRLYIALHYQIPTGFASDTDIVSFLNGSINREEYLRRAISEGFVERLFELLHHNIEKANQE
NAALSLKALYTVFLDSPYLKSFEDAVHGFFGFEPYRNIMWLTFAFIKVLDEKEQFLFDLLQNPSFLPITADISRRLLVQL
GRIETDDPRLKEEQWLSPEKGEEYLKTWSDMVASQLLDGGLADSIHASHIYFVFYRVSKEKTRQVFSKWINEELGIEKIA
KLVGRCGSDSTNGPYTEISEDSLSELLDYQRLKELADVELSNTSQTLSNYLKAVYSSVLTGEKYYLNDATKGEKF

Sequences:

>Translated_715_residues
MGSYHNDQPITGESESPDLLRREAFSEHLATVLRIAPSDDCMTASLEGEWGYGKTSVINLVKKSLGNQENGPVIVEYNPW
LAGKAEALVQDFLVQFSSQLNIPDRPKEGLKAAKELLAYSELFNAMKFIPGVEPWASTVQGVFKVVGGATKKISKLKELD
LIGRKNKINDLLKKLGVSIVVIIDDIDRLTPDEAFQVVRLVKAVADFPGTSFLLSFDPTYLASSLEKHGIENSNQYIDKV
VQLRISLPLIAHNDLQKLADIELQNLSDKSLTDSFERDQERLSYLYHSYVKYLIRNPRELKRTFNHLRFVLSQTEGNVCF
TDLYCLSVLAIKAQEIYHSLKESPEYYVGRKFDDSLAFDKREEVVEKNKDKRDSIITRAPGKDVVYLKGILKELFPLLEG
SGYSMYGSDYDQCGRVASEKRLYIALHYQIPTGFASDTDIVSFLNGSINREEYLRRAISEGFVERLFELLHHNIEKANQE
NAALSLKALYTVFLDSPYLKSFEDAVHGFFGFEPYRNIMWLTFAFIKVLDEKEQFLFDLLQNPSFLPITADISRRLLVQL
GRIETDDPRLKEEQWLSPEKGEEYLKTWSDMVASQLLDGGLADSIHASHIYFVFYRVSKEKTRQVFSKWINEELGIEKIA
KLVGRCGSDSTNGPYTEISEDSLSELLDYQRLKELADVELSNTSQTLSNYLKAVYSSVLTGEKYYLNDATKGEKF
>Mature_714_residues
GSYHNDQPITGESESPDLLRREAFSEHLATVLRIAPSDDCMTASLEGEWGYGKTSVINLVKKSLGNQENGPVIVEYNPWL
AGKAEALVQDFLVQFSSQLNIPDRPKEGLKAAKELLAYSELFNAMKFIPGVEPWASTVQGVFKVVGGATKKISKLKELDL
IGRKNKINDLLKKLGVSIVVIIDDIDRLTPDEAFQVVRLVKAVADFPGTSFLLSFDPTYLASSLEKHGIENSNQYIDKVV
QLRISLPLIAHNDLQKLADIELQNLSDKSLTDSFERDQERLSYLYHSYVKYLIRNPRELKRTFNHLRFVLSQTEGNVCFT
DLYCLSVLAIKAQEIYHSLKESPEYYVGRKFDDSLAFDKREEVVEKNKDKRDSIITRAPGKDVVYLKGILKELFPLLEGS
GYSMYGSDYDQCGRVASEKRLYIALHYQIPTGFASDTDIVSFLNGSINREEYLRRAISEGFVERLFELLHHNIEKANQEN
AALSLKALYTVFLDSPYLKSFEDAVHGFFGFEPYRNIMWLTFAFIKVLDEKEQFLFDLLQNPSFLPITADISRRLLVQLG
RIETDDPRLKEEQWLSPEKGEEYLKTWSDMVASQLLDGGLADSIHASHIYFVFYRVSKEKTRQVFSKWINEELGIEKIAK
LVGRCGSDSTNGPYTEISEDSLSELLDYQRLKELADVELSNTSQTLSNYLKAVYSSVLTGEKYYLNDATKGEKF

Specific function: Responsible for the exclusion of phage T7 by plasmid F. Growth of bacteriophage T7 is inhibited in cells of E.coli that carries the plasmid F [H]

COG id: COG4928

COG function: function code R; Predicted P-loop ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KAP NTPase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011646 [H]

Pfam domain/function: PF07693 KAP_NTPase [H]

EC number: NA

Molecular weight: Translated: 81367; Mature: 81236

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSYHNDQPITGESESPDLLRREAFSEHLATVLRIAPSDDCMTASLEGEWGYGKTSVINL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHH
VKKSLGNQENGPVIVEYNPWLAGKAEALVQDFLVQFSSQLNIPDRPKEGLKAAKELLAYS
HHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ELFNAMKFIPGVEPWASTVQGVFKVVGGATKKISKLKELDLIGRKNKINDLLKKLGVSIV
HHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
VIIDDIDRLTPDEAFQVVRLVKAVADFPGTSFLLSFDPTYLASSLEKHGIENSNQYIDKV
EEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHH
VQLRISLPLIAHNDLQKLADIELQNLSDKSLTDSFERDQERLSYLYHSYVKYLIRNPREL
HHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
KRTFNHLRFVLSQTEGNVCFTDLYCLSVLAIKAQEIYHSLKESPEYYVGRKFDDSLAFDK
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHCCCH
REEVVEKNKDKRDSIITRAPGKDVVYLKGILKELFPLLEGSGYSMYGSDYDQCGRVASEK
HHHHHHCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
RLYIALHYQIPTGFASDTDIVSFLNGSINREEYLRRAISEGFVERLFELLHHNIEKANQE
EEEEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
NAALSLKALYTVFLDSPYLKSFEDAVHGFFGFEPYRNIMWLTFAFIKVLDEKEQFLFDLL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNPSFLPITADISRRLLVQLGRIETDDPRLKEEQWLSPEKGEEYLKTWSDMVASQLLDGG
CCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
LADSIHASHIYFVFYRVSKEKTRQVFSKWINEELGIEKIAKLVGRCGSDSTNGPYTEISE
CCCHHHHCEEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCH
DSLSELLDYQRLKELADVELSNTSQTLSNYLKAVYSSVLTGEKYYLNDATKGEKF
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure 
GSYHNDQPITGESESPDLLRREAFSEHLATVLRIAPSDDCMTASLEGEWGYGKTSVINL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHH
VKKSLGNQENGPVIVEYNPWLAGKAEALVQDFLVQFSSQLNIPDRPKEGLKAAKELLAYS
HHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ELFNAMKFIPGVEPWASTVQGVFKVVGGATKKISKLKELDLIGRKNKINDLLKKLGVSIV
HHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
VIIDDIDRLTPDEAFQVVRLVKAVADFPGTSFLLSFDPTYLASSLEKHGIENSNQYIDKV
EEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHH
VQLRISLPLIAHNDLQKLADIELQNLSDKSLTDSFERDQERLSYLYHSYVKYLIRNPREL
HHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
KRTFNHLRFVLSQTEGNVCFTDLYCLSVLAIKAQEIYHSLKESPEYYVGRKFDDSLAFDK
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHCCCH
REEVVEKNKDKRDSIITRAPGKDVVYLKGILKELFPLLEGSGYSMYGSDYDQCGRVASEK
HHHHHHCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
RLYIALHYQIPTGFASDTDIVSFLNGSINREEYLRRAISEGFVERLFELLHHNIEKANQE
EEEEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
NAALSLKALYTVFLDSPYLKSFEDAVHGFFGFEPYRNIMWLTFAFIKVLDEKEQFLFDLL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNPSFLPITADISRRLLVQLGRIETDDPRLKEEQWLSPEKGEEYLKTWSDMVASQLLDGG
CCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
LADSIHASHIYFVFYRVSKEKTRQVFSKWINEELGIEKIAKLVGRCGSDSTNGPYTEISE
CCCHHHHCEEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCH
DSLSELLDYQRLKELADVELSNTSQTLSNYLKAVYSSVLTGEKYYLNDATKGEKF
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 5283385 [H]