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The map label for this gene is rsuA [H]

Identifier: 119775917

GI number: 119775917

Start: 3327580

End: 3328335

Strand: Reverse

Name: rsuA [H]

Synonym: Sama_2785

Alternate gene names: 119775917

Gene position: 3328335-3327580 (Counterclockwise)

Preceding gene: 119775918

Following gene: 119775915

Centisome position: 77.29

GC content: 58.47

Gene sequence:

>756_bases
ATGATTTCCCCCCGGGGCAGACTCGATAGTTTTTTGGCCAAAACCCTCAATATCCCCAAAAAAGCGGTGCGCGAACTGCT
TAAAGCCGAGCGGGTGACTGTGGATGGCAAGGTAGCGCTGGAGATGGCGCTGCAAATCGGCCCGTTCAGTCAGATTTACC
TCGGCGATACCGCCCTTCGCGCCGACACACCGCGCTACCTGATGCTCAATAAACCCGTGGGCGTGGTATCGGCTACCAAA
GACAAAGAGCACAAAACCGTGCTCGACCTGATCCCCGAGGCGCTCAGAGCCGGACTGCACATAGTCGGCAGGCTGGATTT
GAATACCTCGGGACTGGTGCTTTTATCCAACGACAGCCGCTGGTCTGAATCCATGATGCAGCCCGGTGCCCATGTGCCCA
AGGAATATTGGGTGACCCTGGAACATCCGCTGACCGAGGACTATATCGCCGCTTTTGAGCGCGGCTTTTACTTTGAGACT
GAAGGCATCACCACCCTGCCTGCCAGGCTTGCCATCCTCGGTGAGCGTGAGGCGAGGGTGGAGCTGTGCGAAGGCAAGTA
CCACCAAATCAAGCGTATGTTTGGCCGCTTTCAAAACCCGGTTGTTGCACTGCACCGGCACCGAATCGGCGCCCTGATGC
TGGATCCCGGGCTCGCCCCCGGGCAGTGGCGACCACTCACCGACGATGAAAAGCTGGCTTGCCTGAAGGGCGCCAAGCCG
CCAAACGACTTCAGGCCGATCGCCGAGAACCCATAA

Upstream 100 bases:

>100_bases
GGGAAGGCATTACTGTGGAGCGCAGCGAACACATTATTGAAGGGGCGCGCCGCTGCGCCTATCGCATCACTGCGGCCATG
GCCGATTAAGGCGCAGAACT

Downstream 100 bases:

>100_bases
AAAAGACTGAAATTTCAGTCTTTTTTTACGCCCGGATAAGGGGGATTGGCTCAGGTCCTGGCCGGTTTAAGGGAGATCCA
CAGCTTGATGTGGCCTTTGA

Product: pseudouridine synthase, Rsu

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MISPRGRLDSFLAKTLNIPKKAVRELLKAERVTVDGKVALEMALQIGPFSQIYLGDTALRADTPRYLMLNKPVGVVSATK
DKEHKTVLDLIPEALRAGLHIVGRLDLNTSGLVLLSNDSRWSESMMQPGAHVPKEYWVTLEHPLTEDYIAAFERGFYFET
EGITTLPARLAILGEREARVELCEGKYHQIKRMFGRFQNPVVALHRHRIGALMLDPGLAPGQWRPLTDDEKLACLKGAKP
PNDFRPIAENP

Sequences:

>Translated_251_residues
MISPRGRLDSFLAKTLNIPKKAVRELLKAERVTVDGKVALEMALQIGPFSQIYLGDTALRADTPRYLMLNKPVGVVSATK
DKEHKTVLDLIPEALRAGLHIVGRLDLNTSGLVLLSNDSRWSESMMQPGAHVPKEYWVTLEHPLTEDYIAAFERGFYFET
EGITTLPARLAILGEREARVELCEGKYHQIKRMFGRFQNPVVALHRHRIGALMLDPGLAPGQWRPLTDDEKLACLKGAKP
PNDFRPIAENP
>Mature_251_residues
MISPRGRLDSFLAKTLNIPKKAVRELLKAERVTVDGKVALEMALQIGPFSQIYLGDTALRADTPRYLMLNKPVGVVSATK
DKEHKTVLDLIPEALRAGLHIVGRLDLNTSGLVLLSNDSRWSESMMQPGAHVPKEYWVTLEHPLTEDYIAAFERGFYFET
EGITTLPARLAILGEREARVELCEGKYHQIKRMFGRFQNPVVALHRHRIGALMLDPGLAPGQWRPLTDDEKLACLKGAKP
PNDFRPIAENP

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=225, Percent_Identity=41.7777777777778, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1790453, Length=245, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787524, Length=230, Percent_Identity=28.695652173913, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87081838, Length=184, Percent_Identity=30.9782608695652, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28112; Mature: 28112

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPRGRLDSFLAKTLNIPKKAVRELLKAERVTVDGKVALEMALQIGPFSQIYLGDTALR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHEEEHHHHCCCCCEEEECCEEEC
ADTPRYLMLNKPVGVVSATKDKEHKTVLDLIPEALRAGLHIVGRLDLNTSGLVLLSNDSR
CCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEECCCH
WSESMMQPGAHVPKEYWVTLEHPLTEDYIAAFERGFYFETEGITTLPARLAILGEREARV
HHHHHHCCCCCCCHHHEEEECCCCCHHHHHHHHCCCEEECCCCEECCHHHEEECCCHHHH
ELCEGKYHQIKRMFGRFQNPVVALHRHRIGALMLDPGLAPGQWRPLTDDEKLACLKGAKP
HHHCCHHHHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCC
PNDFRPIAENP
CCCCCCCCCCC
>Mature Secondary Structure
MISPRGRLDSFLAKTLNIPKKAVRELLKAERVTVDGKVALEMALQIGPFSQIYLGDTALR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHEEEHHHHCCCCCEEEECCEEEC
ADTPRYLMLNKPVGVVSATKDKEHKTVLDLIPEALRAGLHIVGRLDLNTSGLVLLSNDSR
CCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEECCCH
WSESMMQPGAHVPKEYWVTLEHPLTEDYIAAFERGFYFETEGITTLPARLAILGEREARV
HHHHHHCCCCCCCHHHEEEECCCCCHHHHHHHHCCCEEECCCCEECCHHHEEECCCHHHH
ELCEGKYHQIKRMFGRFQNPVVALHRHRIGALMLDPGLAPGQWRPLTDDEKLACLKGAKP
HHHCCHHHHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCC
PNDFRPIAENP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]