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The map label for this gene is sdaA [H]

Identifier: 119775903

GI number: 119775903

Start: 3310994

End: 3312388

Strand: Reverse

Name: sdaA [H]

Synonym: Sama_2771

Alternate gene names: 119775903

Gene position: 3312388-3310994 (Counterclockwise)

Preceding gene: 119775904

Following gene: 119775901

Centisome position: 76.92

GC content: 57.71

Gene sequence:

>1395_bases
ATGGCGTCGCCTCTGTATTTGGAGTCTTCCATGTTCAGTACCTTTGATATTTACAAGATTGGTATCGGGCCTTCGAGCTC
CCACACTGTGGGCCCCATGAAGGCGGCGAAAGCCTTTGTGGATAGGCTTGATACCCTTAATCTTTCAGGTCGGATTGATA
GCCTGAAAGCCGACGTGTACGGGTCCCTGTCGCTCACCGGCAAGGGCCACCACACTGACATCGCCATTGTGCTTGGCCTG
ATGGGCCAAAGCCCGGAATGTGTGGATATTGATGCCATTGGGGCCACCATAGCGGCCGTGAATGAATCCGAAACCCTGTG
GCTTGAAAGCGGCAGACCGGTGCGATTTGCCCGGGATGCCATGGTGTTTCATCCCCATGCCTTGCCACTGCACGAAAACG
CCATGACCCTGAGCGCCTTTGCCGGGGATGAGCTGCTGGCATCCAACACCTACTACTCCATAGGTGGTGGTTTTGTGGTG
GAAGAGGCCGACTTTGGCAAGGCTCAGATTGGTGAGACCTTGTTTCCGTATCCTTTCGAGGATGCCGAACGGCTGGTGCA
GCTATGCCGCGCCGAAGGGGTGAGCATTTCTACCTTGATGCTGGCAAATGAAAAGGACTGCCACAGTGAAGAGTATATTT
ACCGCGGATTTCGCGATATTTGGCTGACCATGAAGCAGGCCATTGACAGAGGCTGTCACACCGAAGGTGTGCTTGCCGGG
CCGCTGCGGGTACCTCGCCGGGCCCCGGCGCTGCTTAAACAGCTGGAAACCAGCGGCCGTATCAGCGCCGACCCAATGGA
AATTGTGGATTGGGTCAACCTGTTTGCCCTCGCCGTAAGTGAAGAAAACGCCGCCGGTGGCCGGGTGGTGACAGCGCCCA
CCAACGGTGCGGCTGGTATTATTCCGGCGGTGATGGCCTACTTTGACCGCTTTATTCAGCCCCTGGGGCAAAAGGAGTAC
AGCCGATTTTTGCTGGCATCTGCCGCCATCGGCAGCCTGTATAAGCGTAATGCCTCTATCTCTGGCGCTGAAGTGGGTTG
CCAGGGCGAAGTGGGCGTAGCCTGCTCCATGGCCGCGGCGGGCCTGGCCGAACTCATGGGTGCAAGCCCAGCGCAGGTGT
GTATGGCCGCCGAAATTGCCATGGAACATAACCTGGGGCTGACGTGCGATCCGGTGGCAGGCCAGGTGCAGGTGCCCTGT
ATCGAGCGTAATGCCATTGCGGCGGTGAAAGCCGTGAATGCTGCGCGCATGGCGCTGCGGCGCACTTCTGATCCTCGTGT
GAGCCTGGATAAGGTGATTGCCACCATGTACGAAACCGGCAAAGACATGCACGCCAAGTATCGTGAAACCAGCCTGGGCG
GCCTTGCCATCAAGGTCACCAGCATCTGTGACTAA

Upstream 100 bases:

>100_bases
CGTGGCCGCATCAGCAATATCTTCGTGATCATTGCCGGTTTACTGTCGATGACAGCCATCCTCTACGGGCTGACCGCCTG
AGTTGATTCAGGGGCGGCCC

Downstream 100 bases:

>100_bases
ACTGAAAAAGGCCATCAACGATGGCCTTTTTTATATGTAAAATTAACCTGTTATCAGCTCTTTGGCTTTTCTTCGTACAT
CAGGTAGTAGCTCTTCTCCA

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH 1; L-serine deaminase 1; L-SD1 [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MASPLYLESSMFSTFDIYKIGIGPSSSHTVGPMKAAKAFVDRLDTLNLSGRIDSLKADVYGSLSLTGKGHHTDIAIVLGL
MGQSPECVDIDAIGATIAAVNESETLWLESGRPVRFARDAMVFHPHALPLHENAMTLSAFAGDELLASNTYYSIGGGFVV
EEADFGKAQIGETLFPYPFEDAERLVQLCRAEGVSISTLMLANEKDCHSEEYIYRGFRDIWLTMKQAIDRGCHTEGVLAG
PLRVPRRAPALLKQLETSGRISADPMEIVDWVNLFALAVSEENAAGGRVVTAPTNGAAGIIPAVMAYFDRFIQPLGQKEY
SRFLLASAAIGSLYKRNASISGAEVGCQGEVGVACSMAAAGLAELMGASPAQVCMAAEIAMEHNLGLTCDPVAGQVQVPC
IERNAIAAVKAVNAARMALRRTSDPRVSLDKVIATMYETGKDMHAKYRETSLGGLAIKVTSICD

Sequences:

>Translated_464_residues
MASPLYLESSMFSTFDIYKIGIGPSSSHTVGPMKAAKAFVDRLDTLNLSGRIDSLKADVYGSLSLTGKGHHTDIAIVLGL
MGQSPECVDIDAIGATIAAVNESETLWLESGRPVRFARDAMVFHPHALPLHENAMTLSAFAGDELLASNTYYSIGGGFVV
EEADFGKAQIGETLFPYPFEDAERLVQLCRAEGVSISTLMLANEKDCHSEEYIYRGFRDIWLTMKQAIDRGCHTEGVLAG
PLRVPRRAPALLKQLETSGRISADPMEIVDWVNLFALAVSEENAAGGRVVTAPTNGAAGIIPAVMAYFDRFIQPLGQKEY
SRFLLASAAIGSLYKRNASISGAEVGCQGEVGVACSMAAAGLAELMGASPAQVCMAAEIAMEHNLGLTCDPVAGQVQVPC
IERNAIAAVKAVNAARMALRRTSDPRVSLDKVIATMYETGKDMHAKYRETSLGGLAIKVTSICD
>Mature_463_residues
ASPLYLESSMFSTFDIYKIGIGPSSSHTVGPMKAAKAFVDRLDTLNLSGRIDSLKADVYGSLSLTGKGHHTDIAIVLGLM
GQSPECVDIDAIGATIAAVNESETLWLESGRPVRFARDAMVFHPHALPLHENAMTLSAFAGDELLASNTYYSIGGGFVVE
EADFGKAQIGETLFPYPFEDAERLVQLCRAEGVSISTLMLANEKDCHSEEYIYRGFRDIWLTMKQAIDRGCHTEGVLAGP
LRVPRRAPALLKQLETSGRISADPMEIVDWVNLFALAVSEENAAGGRVVTAPTNGAAGIIPAVMAYFDRFIQPLGQKEYS
RFLLASAAIGSLYKRNASISGAEVGCQGEVGVACSMAAAGLAELMGASPAQVCMAAEIAMEHNLGLTCDPVAGQVQVPCI
ERNAIAAVKAVNAARMALRRTSDPRVSLDKVIATMYETGKDMHAKYRETSLGGLAIKVTSICD

Specific function: Deaminates also threonine, particularly when it is present in high concentration [H]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1788116, Length=456, Percent_Identity=65.7894736842105, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1789161, Length=456, Percent_Identity=64.6929824561403, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI48994925, Length=452, Percent_Identity=64.6017699115044, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 49547; Mature: 49416

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPLYLESSMFSTFDIYKIGIGPSSSHTVGPMKAAKAFVDRLDTLNLSGRIDSLKADVY
CCCCEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCEE
GSLSLTGKGHHTDIAIVLGLMGQSPECVDIDAIGATIAAVNESETLWLESGRPVRFARDA
EEEEECCCCCCCHHEEEEECCCCCCCEEEEECCCCEEEEECCCCEEEEECCCCEEEECCE
MVFHPHALPLHENAMTLSAFAGDELLASNTYYSIGGGFVVEEADFGKAQIGETLFPYPFE
EEECCCCCCCCCCCEEEEEECCCHHEECCCEEECCCCEEEECCCCCHHHHCCCCCCCCCH
DAERLVQLCRAEGVSISTLMLANEKDCHSEEYIYRGFRDIWLTMKQAIDRGCHTEGVLAG
HHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
PLRVPRRAPALLKQLETSGRISADPMEIVDWVNLFALAVSEENAAGGRVVTAPTNGAAGI
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCCHH
IPAVMAYFDRFIQPLGQKEYSRFLLASAAIGSLYKRNASISGAEVGCQGEVGVACSMAAA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
GLAELMGASPAQVCMAAEIAMEHNLGLTCDPVAGQVQVPCIERNAIAAVKAVNAARMALR
HHHHHHCCCHHHHHHHHHHHHHCCCCCEECCCCCCEECCEECCCHHHHHHHHHHHHHHHH
RTSDPRVSLDKVIATMYETGKDMHAKYRETSLGGLAIKVTSICD
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure 
ASPLYLESSMFSTFDIYKIGIGPSSSHTVGPMKAAKAFVDRLDTLNLSGRIDSLKADVY
CCCEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCEE
GSLSLTGKGHHTDIAIVLGLMGQSPECVDIDAIGATIAAVNESETLWLESGRPVRFARDA
EEEEECCCCCCCHHEEEEECCCCCCCEEEEECCCCEEEEECCCCEEEEECCCCEEEECCE
MVFHPHALPLHENAMTLSAFAGDELLASNTYYSIGGGFVVEEADFGKAQIGETLFPYPFE
EEECCCCCCCCCCCEEEEEECCCHHEECCCEEECCCCEEEECCCCCHHHHCCCCCCCCCH
DAERLVQLCRAEGVSISTLMLANEKDCHSEEYIYRGFRDIWLTMKQAIDRGCHTEGVLAG
HHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
PLRVPRRAPALLKQLETSGRISADPMEIVDWVNLFALAVSEENAAGGRVVTAPTNGAAGI
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCCHH
IPAVMAYFDRFIQPLGQKEYSRFLLASAAIGSLYKRNASISGAEVGCQGEVGVACSMAAA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
GLAELMGASPAQVCMAAEIAMEHNLGLTCDPVAGQVQVPCIERNAIAAVKAVNAARMALR
HHHHHHCCCHHHHHHHHHHHHHCCCCCEECCCCCCEECCEECCCHHHHHHHHHHHHHHHH
RTSDPRVSLDKVIATMYETGKDMHAKYRETSLGGLAIKVTSICD
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2504697; 8436113; 9097040; 9278503 [H]