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The map label for this gene is gcvH

Identifier: 119775852

GI number: 119775852

Start: 3255155

End: 3255544

Strand: Reverse

Name: gcvH

Synonym: Sama_2720

Alternate gene names: 119775852

Gene position: 3255544-3255155 (Counterclockwise)

Preceding gene: 119775853

Following gene: 119775851

Centisome position: 75.6

GC content: 53.33

Gene sequence:

>390_bases
ATGAGCAATATCCCGTCCGAACTCAAGTATGCTTCTTCCCACGAATGGATCCGCAAGGAAGACGACGGCACCTACACCGT
AGGTATCACTGAGCACGCTCAGGAACTGCTGGGTGACATGGTATTCGTTGAGCTGCCTGAAGTGGGTGACACTGTGTCTG
CCGGTGAAGACTGCGCCGTAGCTGAGTCTGTAAAAGCTGCCTCTGACATCTATGCCCCTATCTCCGGTGAAGTGATTGCC
GTAAACGAGTCTCTGGAAGATTCTCCAGAGCTGGTAAACAGCGACGCTTACGGTGACGGCTGGTTCTTCCGCATCAAGCC
TTCTGACGAGTCTGAGCTGGACAGCCTGCTCGACGCCGAAGGTTATCAAGCAGTTATCGACGAGGAATAA

Upstream 100 bases:

>100_bases
GAAATGCGCAAGAAGCGTGTGACGGTGCGTGTCATCGCACCCAGCTTCGTACGCAATGGAAAACAAGCATTTTAAAAGAC
CCGATTAAGGAACAAGAACA

Downstream 100 bases:

>100_bases
TCCTCGCCAGGAAAAACGAAGCCCCCCGGGGCTTCGTTTGTTTTATCCGACATAAAGCAGATCGGTCGCAATTGAGATCG
GCGTAATTTGAAAGGTTTAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSNIPSELKYASSHEWIRKEDDGTYTVGITEHAQELLGDMVFVELPEVGDTVSAGEDCAVAESVKAASDIYAPISGEVIA
VNESLEDSPELVNSDAYGDGWFFRIKPSDESELDSLLDAEGYQAVIDEE

Sequences:

>Translated_129_residues
MSNIPSELKYASSHEWIRKEDDGTYTVGITEHAQELLGDMVFVELPEVGDTVSAGEDCAVAESVKAASDIYAPISGEVIA
VNESLEDSPELVNSDAYGDGWFFRIKPSDESELDSLLDAEGYQAVIDEE
>Mature_128_residues
SNIPSELKYASSHEWIRKEDDGTYTVGITEHAQELLGDMVFVELPEVGDTVSAGEDCAVAESVKAASDIYAPISGEVIAV
NESLEDSPELVNSDAYGDGWFFRIKPSDESELDSLLDAEGYQAVIDEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=46.218487394958, Blast_Score=114, Evalue=3e-26,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=45.3781512605042, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=75.968992248062, Blast_Score=209, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=44.1666666666667, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=47.787610619469, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=42.4, Blast_Score=106, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=41.6, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_SHEAM (A1S966)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928592.1
- ProteinModelPortal:   A1S966
- SMR:   A1S966
- STRING:   A1S966
- GeneID:   4604967
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2720
- NMPDR:   fig|326297.7.peg.2550
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   A1S966
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14021; Mature: 13890

Theoretical pI: Translated: 3.59; Mature: 3.59

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPSELKYASSHEWIRKEDDGTYTVGITEHAQELLGDMVFVELPEVGDTVSAGEDCAV
CCCCCCHHHHCCCCCCEEECCCCEEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHH
AESVKAASDIYAPISGEVIAVNESLEDSPELVNSDAYGDGWFFRIKPSDESELDSLLDAE
HHHHHHHHHCCCCCCCCEEEECCCCCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
GYQAVIDEE
CCEEEECCC
>Mature Secondary Structure 
SNIPSELKYASSHEWIRKEDDGTYTVGITEHAQELLGDMVFVELPEVGDTVSAGEDCAV
CCCCCHHHHCCCCCCEEECCCCEEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHH
AESVKAASDIYAPISGEVIAVNESLEDSPELVNSDAYGDGWFFRIKPSDESELDSLLDAE
HHHHHHHHHCCCCCCCCEEEECCCCCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
GYQAVIDEE
CCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA