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The map label for this gene is puuD [H]

Identifier: 119775777

GI number: 119775777

Start: 3169216

End: 3169980

Strand: Reverse

Name: puuD [H]

Synonym: Sama_2645

Alternate gene names: 119775777

Gene position: 3169980-3169216 (Counterclockwise)

Preceding gene: 119775778

Following gene: 119775776

Centisome position: 73.62

GC content: 54.25

Gene sequence:

>765_bases
ATGTCTGACGCCACGATACCCCTTATTGGTGTCTCTGCCTGTAATACCCCGATTGGACTGCAGACGTTTAATACGGTGGG
AGAAAAATATCTTTTAGGTGTGATTAATGGCACTGGCGGTTGGCCGCTGATCATTCCTTCTATTGGCGATGGCATGCCAA
CGGAACTCTTGCTTGAGCGTCTGGACGGCATACTGTTCACAGGTTCACCTTCCAATGTCGAACCACATCACTATTCGGGG
CCGGCCAGCGAGCCGGGAACTCATCACGATCCCCGCCGTGATGCCACCACCTTGCCTTTGATTAAGGCGGCTATCGCTGC
GGGCGTGCCGGTACTGGGTATCTGTCGCGGCTTCCAGGAAATGAACGTGGCCTTTGGCGGCAGTTTGCACCAAAAGTTAC
ATGAAACGGGTGTGTTTGAAGAGCACAGGGAAGACAGAACTGCGCCACTTGAGGTGCAGTATGGTCTGGCCCACACGGTG
ACTCTGGAGCCCGGGGGGGTAATTTTCGAAGCCTGGGGCCGCAGCTCGGCGGAAGTGAATTCCGTCCATACTCAGGGCGT
TGAACGTCTGGGTAACGGGTTGCGGCCAGAAGCCTATGCACCGGATGGCTTGATAGAAGCCTTCTCTGTTACAGATGCCA
AAAATTTTGCCCTGGGTGTGCAATTTCATCCCGAATGGAAAGTGGCCGACAATGCTTTTTATCTGTCGATTTTCAATGCC
TTCGGTGACGCGTGTCGACGCAGGGCCCAAGAGCGAGCGAGATAA

Upstream 100 bases:

>100_bases
ATTGCCTCGTTCAATATTTCCGTGTACTGTGTGAAAAAATGAACGCCGAGTTTGAGGGCGATCACAAAACTCAAACACTG
AATCAACTGCAAGGTGATGT

Downstream 100 bases:

>100_bases
ATCATGGAAAAGCTGATTGCGTTTTTAAAAGAAAAAAAGATAACGGAAGTAGAGTGTGTTATCAGCGATATGACGGGGAT
TGCCCGCGGTAAAATCGCGC

Product: glutamine amidotransferase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSDATIPLIGVSACNTPIGLQTFNTVGEKYLLGVINGTGGWPLIIPSIGDGMPTELLLERLDGILFTGSPSNVEPHHYSG
PASEPGTHHDPRRDATTLPLIKAAIAAGVPVLGICRGFQEMNVAFGGSLHQKLHETGVFEEHREDRTAPLEVQYGLAHTV
TLEPGGVIFEAWGRSSAEVNSVHTQGVERLGNGLRPEAYAPDGLIEAFSVTDAKNFALGVQFHPEWKVADNAFYLSIFNA
FGDACRRRAQERAR

Sequences:

>Translated_254_residues
MSDATIPLIGVSACNTPIGLQTFNTVGEKYLLGVINGTGGWPLIIPSIGDGMPTELLLERLDGILFTGSPSNVEPHHYSG
PASEPGTHHDPRRDATTLPLIKAAIAAGVPVLGICRGFQEMNVAFGGSLHQKLHETGVFEEHREDRTAPLEVQYGLAHTV
TLEPGGVIFEAWGRSSAEVNSVHTQGVERLGNGLRPEAYAPDGLIEAFSVTDAKNFALGVQFHPEWKVADNAFYLSIFNA
FGDACRRRAQERAR
>Mature_253_residues
SDATIPLIGVSACNTPIGLQTFNTVGEKYLLGVINGTGGWPLIIPSIGDGMPTELLLERLDGILFTGSPSNVEPHHYSGP
ASEPGTHHDPRRDATTLPLIKAAIAAGVPVLGICRGFQEMNVAFGGSLHQKLHETGVFEEHREDRTAPLEVQYGLAHTVT
LEPGGVIFEAWGRSSAEVNSVHTQGVERLGNGLRPEAYAPDGLIEAFSVTDAKNFALGVQFHPEWKVADNAFYLSIFNAF
GDACRRRAQERAR

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=250, Percent_Identity=42.8, Blast_Score=183, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27336; Mature: 27205

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDATIPLIGVSACNTPIGLQTFNTVGEKYLLGVINGTGGWPLIIPSIGDGMPTELLLER
CCCCCEEEEEEECCCCCCCHHHHHHCCCEEEEEEEECCCCCEEEECCCCCCCCHHHHHHH
LDGILFTGSPSNVEPHHYSGPASEPGTHHDPRRDATTLPLIKAAIAAGVPVLGICRGFQE
HCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCHHH
MNVAFGGSLHQKLHETGVFEEHREDRTAPLEVQYGLAHTVTLEPGGVIFEAWGRSSAEVN
HHHHHCCHHHHHHHHCCCHHHHHHCCCCCEEEEECCEEEEEECCCCEEEEECCCCCCHHH
SVHTQGVERLGNGLRPEAYAPDGLIEAFSVTDAKNFALGVQFHPEWKVADNAFYLSIFNA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCEEEECCEEEEHHHHH
FGDACRRRAQERAR
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SDATIPLIGVSACNTPIGLQTFNTVGEKYLLGVINGTGGWPLIIPSIGDGMPTELLLER
CCCCEEEEEEECCCCCCCHHHHHHCCCEEEEEEEECCCCCEEEECCCCCCCCHHHHHHH
LDGILFTGSPSNVEPHHYSGPASEPGTHHDPRRDATTLPLIKAAIAAGVPVLGICRGFQE
HCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCHHH
MNVAFGGSLHQKLHETGVFEEHREDRTAPLEVQYGLAHTVTLEPGGVIFEAWGRSSAEVN
HHHHHCCHHHHHHHHCCCHHHHHHCCCCCEEEEECCEEEEEECCCCEEEEECCCCCCHHH
SVHTQGVERLGNGLRPEAYAPDGLIEAFSVTDAKNFALGVQFHPEWKVADNAFYLSIFNA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCEEEECCEEEEHHHHH
FGDACRRRAQERAR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]