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The map label for this gene is puuA [H]

Identifier: 119775776

GI number: 119775776

Start: 3167857

End: 3169212

Strand: Reverse

Name: puuA [H]

Synonym: Sama_2644

Alternate gene names: 119775776

Gene position: 3169212-3167857 (Counterclockwise)

Preceding gene: 119775777

Following gene: 119775775

Centisome position: 73.6

GC content: 55.01

Gene sequence:

>1356_bases
ATGGAAAAGCTGATTGCGTTTTTAAAAGAAAAAAAGATAACGGAAGTAGAGTGTGTTATCAGCGATATGACGGGGATTGC
CCGCGGTAAAATCGCGCCGGTAGACAAATTTATCGCTGAGCAGGGCATGCGTTTGCCCGAGAGCGTACTGCTGCAAACGG
TGACCGGCGACTATGTGAATGACGATACTTATTATGAGCTGCTGAACGAAGCCGATGTGGACTTCCTCTGTGTACCCGAT
GAAAACGCCGTGTTCGAGTTGCCCTGGTGTGTGGAAGCCACGGCTCAGGTGATCCACGATGTGTACGACCGCATGGGGAA
CCCCATCGAACTGTCACCGCGTAACGTACTGAAAAAAGTGCTTAAACTGTACGAAGAAAAGGGGTGGGAACCCGTGGTTG
CCCCCGAGATGGAGTTTTATCTGGTGGCGCGCAATGGGGATCCGGATCTGCCGCTGAATCCGCCACTGGGACGTTCAGGT
CTCCCTGAAGCCGGCCGCCAATCGTTCTCCATTGATGCGGCCAACGAATACGACCCGCTGTTTGAAGACATGTATGAGTG
GTGCGAGGCCCAGGGGCTGTTTATCGACACCCTGATCCACGAAGATGGCCCGGCGCAGATGGAAATCAACTTCAGTCACG
GTAATGCGCTGTCGCTGGCCGACCAGGTGTTTGTGTTCAAGCGTACCCTGCGTGAAGCGGCGCTCAAGCACAATGTCTGT
GCCACCTTCATGGCCAAACCTGTGACCAACGAGCCGGGCAGTGCCATGCACATTCACCAGAGCGTGGTGGATAAAAAGAC
CGGCAAGAACATTTTCACCAAAGAAGATGGCACCAAGAGTGCTAACTTCCTCGGCTATATTGCCGGTCTGCAACAGTTTA
TCCCTGAATTCCTGCCGTTGATGGCGCCCAGTGTGAACTCCTTCCGCCGTTTCCTGCCGGGCACCTCTGCACCGGTAAAT
CTGGAGTGGGGCGAAGAAAATCGCACCTGTGGTCTGCGTATTCCTGAGTCATCACCGCAAAACCGCCGAATTGAAAACCG
TATTCCAGGCGCCGATGCCAACTGCTATCTGGCCATTGCCGCAAGCTTGCTGGCCGGTTATATCGGCATGGTGGAAGGGC
TTAAACCAACCAACCCGGCCCTGGGGCGTGCCAACGAAAGCCGCACTGCAGACACCAATTGCCTGCCGCTGACCCTGGAA
GAGGCGCTGACGGCCATGGATGAGAGCGACGCGGCGCGCAAATATCTCGGCGATGCCTTCACCACAGGTTTTGTGGCGGT
GAAGCAGGCAGAGCTTGAAAACTTCAGGCGTGTTGTCAGTGCCTGGGAGCGTGAATTCCTGCTGCTGACAGTATAA

Upstream 100 bases:

>100_bases
TCCCGAATGGAAAGTGGCCGACAATGCTTTTTATCTGTCGATTTTCAATGCCTTCGGTGACGCGTGTCGACGCAGGGCCC
AAGAGCGAGCGAGATAAATC

Downstream 100 bases:

>100_bases
AAACACAGGCCGGGGGTGACCCCGGCACTTTAGAGGGAGGGCGAAGATGCAACAACAACAGCAAGATACCATCAGCGCAC
TGCAAGCCATGGATGCCGCA

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MEKLIAFLKEKKITEVECVISDMTGIARGKIAPVDKFIAEQGMRLPESVLLQTVTGDYVNDDTYYELLNEADVDFLCVPD
ENAVFELPWCVEATAQVIHDVYDRMGNPIELSPRNVLKKVLKLYEEKGWEPVVAPEMEFYLVARNGDPDLPLNPPLGRSG
LPEAGRQSFSIDAANEYDPLFEDMYEWCEAQGLFIDTLIHEDGPAQMEINFSHGNALSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTNEPGSAMHIHQSVVDKKTGKNIFTKEDGTKSANFLGYIAGLQQFIPEFLPLMAPSVNSFRRFLPGTSAPVN
LEWGEENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLAGYIGMVEGLKPTNPALGRANESRTADTNCLPLTLE
EALTAMDESDAARKYLGDAFTTGFVAVKQAELENFRRVVSAWEREFLLLTV

Sequences:

>Translated_451_residues
MEKLIAFLKEKKITEVECVISDMTGIARGKIAPVDKFIAEQGMRLPESVLLQTVTGDYVNDDTYYELLNEADVDFLCVPD
ENAVFELPWCVEATAQVIHDVYDRMGNPIELSPRNVLKKVLKLYEEKGWEPVVAPEMEFYLVARNGDPDLPLNPPLGRSG
LPEAGRQSFSIDAANEYDPLFEDMYEWCEAQGLFIDTLIHEDGPAQMEINFSHGNALSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTNEPGSAMHIHQSVVDKKTGKNIFTKEDGTKSANFLGYIAGLQQFIPEFLPLMAPSVNSFRRFLPGTSAPVN
LEWGEENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLAGYIGMVEGLKPTNPALGRANESRTADTNCLPLTLE
EALTAMDESDAARKYLGDAFTTGFVAVKQAELENFRRVVSAWEREFLLLTV
>Mature_451_residues
MEKLIAFLKEKKITEVECVISDMTGIARGKIAPVDKFIAEQGMRLPESVLLQTVTGDYVNDDTYYELLNEADVDFLCVPD
ENAVFELPWCVEATAQVIHDVYDRMGNPIELSPRNVLKKVLKLYEEKGWEPVVAPEMEFYLVARNGDPDLPLNPPLGRSG
LPEAGRQSFSIDAANEYDPLFEDMYEWCEAQGLFIDTLIHEDGPAQMEINFSHGNALSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTNEPGSAMHIHQSVVDKKTGKNIFTKEDGTKSANFLGYIAGLQQFIPEFLPLMAPSVNSFRRFLPGTSAPVN
LEWGEENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLAGYIGMVEGLKPTNPALGRANESRTADTNCLPLTLE
EALTAMDESDAARKYLGDAFTTGFVAVKQAELENFRRVVSAWEREFLLLTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=446, Percent_Identity=27.3542600896861, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI87081870, Length=439, Percent_Identity=34.624145785877, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1790301, Length=475, Percent_Identity=25.6842105263158, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50167; Mature: 50167

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLIAFLKEKKITEVECVISDMTGIARGKIAPVDKFIAEQGMRLPESVLLQTVTGDYVN
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCC
DDTYYELLNEADVDFLCVPDENAVFELPWCVEATAQVIHDVYDRMGNPIELSPRNVLKKV
CHHHHHHHHCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LKLYEEKGWEPVVAPEMEFYLVARNGDPDLPLNPPLGRSGLPEAGRQSFSIDAANEYDPL
HHHHHHCCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCEEECCCCCCCHH
FEDMYEWCEAQGLFIDTLIHEDGPAQMEINFSHGNALSLADQVFVFKRTLREAALKHNVC
HHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPVTNEPGSAMHIHQSVVDKKTGKNIFTKEDGTKSANFLGYIAGLQQFIPEFLPL
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHH
MAPSVNSFRRFLPGTSAPVNLEWGEENRTCGLRIPESSPQNRRIENRIPGADANCYLAIA
HCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHH
ASLLAGYIGMVEGLKPTNPALGRANESRTADTNCLPLTLEEALTAMDESDAARKYLGDAF
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHH
TTGFVAVKQAELENFRRVVSAWEREFLLLTV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MEKLIAFLKEKKITEVECVISDMTGIARGKIAPVDKFIAEQGMRLPESVLLQTVTGDYVN
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCC
DDTYYELLNEADVDFLCVPDENAVFELPWCVEATAQVIHDVYDRMGNPIELSPRNVLKKV
CHHHHHHHHCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LKLYEEKGWEPVVAPEMEFYLVARNGDPDLPLNPPLGRSGLPEAGRQSFSIDAANEYDPL
HHHHHHCCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCEEECCCCCCCHH
FEDMYEWCEAQGLFIDTLIHEDGPAQMEINFSHGNALSLADQVFVFKRTLREAALKHNVC
HHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPVTNEPGSAMHIHQSVVDKKTGKNIFTKEDGTKSANFLGYIAGLQQFIPEFLPL
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHH
MAPSVNSFRRFLPGTSAPVNLEWGEENRTCGLRIPESSPQNRRIENRIPGADANCYLAIA
HCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHH
ASLLAGYIGMVEGLKPTNPALGRANESRTADTNCLPLTLEEALTAMDESDAARKYLGDAF
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHH
TTGFVAVKQAELENFRRVVSAWEREFLLLTV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]