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The map label for this gene is potI [H]

Identifier: 119775771

GI number: 119775771

Start: 3162181

End: 3162996

Strand: Reverse

Name: potI [H]

Synonym: Sama_2639

Alternate gene names: 119775771

Gene position: 3162996-3162181 (Counterclockwise)

Preceding gene: 119775772

Following gene: 119775770

Centisome position: 73.45

GC content: 55.39

Gene sequence:

>816_bases
ATGAAAAAGCTGAGTTTTTCTACCGTGATGTTGTGGTTGGGGCTCATCTTCCTCTATGCCCCCATGTTTATCCTGATTTT
CTACTCCTTCAACGCGTCCAAACTGGTGACCGTGTGGGGTGGATTCTCGGCCAAATGGTATGTGGAGCTGTTCAAGGATC
AGCAAATCCTTGATGCGGTCTGGACCAGTTTGGAAATTGCCTTCCTGTCGGCCTCCATGGCCGTCGTGTTGGGTACCATG
GCGGCGTTCGTGATGACACGTTTCCGTCGCAGCTGGGCCAAGCTGTCGCTGTCAAACATGATAACGGCGCCACTGGTTAT
GCCTGAGGTGATTACGGGTCTGTCTTTGCTGCTGCTGTTTGTGCACATGGCTGACCTGATTGGCTGGCCTGCCGAGCGCG
GTAGACTCACCATTTGGCTGGCGCATTCCACCTTCTGTGCCGCCTACGTGGCTGTGGTGGTGTCGTCCCGTCTGCGTGAA
CTGGACATGTCGATTGAGGAAGCCGCTATGGATCTGGGTGCAACGCCACTCAAGACCTTCTTCCTTATCACTGTTCCCAT
GATTGCGCCGGCACTGACCGCAGGTTGGCTGCTGTCATTCAGCCTGTCTCTGGATGATTTGGTGATTGCGAGCTTTGCGT
CGGGCCCGGGGGCAACGACGCTGCCGATGGTAGTGTTCTCGTCGGTGCGTTTGGGGGTATCTCCCAAGATTAACGCGCTG
GCAACACTCATCATTCTCGCGGTGTCACTGATTGCATTCCTGTCCTGGTATTTTGCCCGCAGGGCAGAAAAGCGCGCAAA
GGCAGCGATGGAGTAG

Upstream 100 bases:

>100_bases
GGCCGGTCGCCTCGGCGCTGGCGATAGTGATGCTGGGACTTTTGATTATCCCCATTACCCTGTTCCATCATTATCAGGCC
CGTGATTTGGAGAAAGAGGC

Downstream 100 bases:

>100_bases
TGACGCATCGCCACTAATAAAACGGTCGCTCAGGCGACCGTTTTTGTTTGTTTGATATTTTCAACAAAAATTCAAAATTA
TCCTTCACTAGCGCTAGAGT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKKLSFSTVMLWLGLIFLYAPMFILIFYSFNASKLVTVWGGFSAKWYVELFKDQQILDAVWTSLEIAFLSASMAVVLGTM
AAFVMTRFRRSWAKLSLSNMITAPLVMPEVITGLSLLLLFVHMADLIGWPAERGRLTIWLAHSTFCAAYVAVVVSSRLRE
LDMSIEEAAMDLGATPLKTFFLITVPMIAPALTAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
ATLIILAVSLIAFLSWYFARRAEKRAKAAME

Sequences:

>Translated_271_residues
MKKLSFSTVMLWLGLIFLYAPMFILIFYSFNASKLVTVWGGFSAKWYVELFKDQQILDAVWTSLEIAFLSASMAVVLGTM
AAFVMTRFRRSWAKLSLSNMITAPLVMPEVITGLSLLLLFVHMADLIGWPAERGRLTIWLAHSTFCAAYVAVVVSSRLRE
LDMSIEEAAMDLGATPLKTFFLITVPMIAPALTAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
ATLIILAVSLIAFLSWYFARRAEKRAKAAME
>Mature_271_residues
MKKLSFSTVMLWLGLIFLYAPMFILIFYSFNASKLVTVWGGFSAKWYVELFKDQQILDAVWTSLEIAFLSASMAVVLGTM
AAFVMTRFRRSWAKLSLSNMITAPLVMPEVITGLSLLLLFVHMADLIGWPAERGRLTIWLAHSTFCAAYVAVVVSSRLRE
LDMSIEEAAMDLGATPLKTFFLITVPMIAPALTAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
ATLIILAVSLIAFLSWYFARRAEKRAKAAME

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=257, Percent_Identity=68.0933852140078, Blast_Score=320, Evalue=6e-89,
Organism=Escherichia coli, GI1787368, Length=242, Percent_Identity=36.7768595041322, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=33.203125, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788764, Length=175, Percent_Identity=31.4285714285714, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29856; Mature: 29856

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSFSTVMLWLGLIFLYAPMFILIFYSFNASKLVTVWGGFSAKWYVELFKDQQILDAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
WTSLEIAFLSASMAVVLGTMAAFVMTRFRRSWAKLSLSNMITAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHMADLIGWPAERGRLTIWLAHSTFCAAYVAVVVSSRLRELDMSIEEAAMDLGATPLKTF
HHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
FLITVPMIAPALTAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHH
ATLIILAVSLIAFLSWYFARRAEKRAKAAME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLSFSTVMLWLGLIFLYAPMFILIFYSFNASKLVTVWGGFSAKWYVELFKDQQILDAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
WTSLEIAFLSASMAVVLGTMAAFVMTRFRRSWAKLSLSNMITAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHMADLIGWPAERGRLTIWLAHSTFCAAYVAVVVSSRLRELDMSIEEAAMDLGATPLKTF
HHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
FLITVPMIAPALTAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRLGVSPKINAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHH
ATLIILAVSLIAFLSWYFARRAEKRAKAAME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]