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The map label for this gene is lolB

Identifier: 119775700

GI number: 119775700

Start: 3074536

End: 3075159

Strand: Direct

Name: lolB

Synonym: Sama_2568

Alternate gene names: 119775700

Gene position: 3074536-3075159 (Clockwise)

Preceding gene: 119775691

Following gene: 119775701

Centisome position: 71.4

GC content: 56.89

Gene sequence:

>624_bases
ATGATTAACTTGCGCCGATTCACAAAATTCACTCTTGCTGGCCTGACCGCCCTTTCTCTGCTCGGTGGTTGCAGCGTCAC
ACCAAAGGTCGCGCTGCAGCCTGTCAGCGTAGAAAACGCCAGTGATGCCAAGGCCTGGGAGCTTAAGGGCAAGCTGCTTA
TTCGCACCAATGGCGACAAGGTTTCGGCCAACCTCTTCTGGCTCAATACCCCTGATAATGCCGAATTAAGGCTGACCAGC
ATGCTGGGCACTACTGTGCTGCTGCTGACCCAAAACCGGGATGGCGCCACCCTGGAAGTGGATGGCAAGCGCTACTCAGA
CCTGAGTCCACAGCGGTTGCTCGACGGTTTAAGTGGTTTTACCCTGCCCATCGATGCCCTGCCCTTTTGGATCACCGGCC
AGCCCATGGCGGGTGATGAGGTGGAGTTCGACACCCTCAATCGCCCCAAGACCATCATCAGTGCCGACGGCGAGTGGACC
ATTAACATTAGCAGCTGGCAGACCCAGAGTGGTGCACCGGTTCCACGGATGCTGGAGCTTACCCACGCCAGCGCTGTTAT
CAAGCTGCAAACCAATGAATGGCAGGCACTGGCCAATGCCACAGGTTCGAAAGGAGCCCGATGA

Upstream 100 bases:

>100_bases
ATGCGGGGGTGAAACCCGTCCGCAGCCAAAAAGCGGGTAAGGCCATTGGTATAATTTTTAAAGCCCTCGTATCATAAGGA
CTTATTTAATTCAGTCATAG

Downstream 100 bases:

>100_bases
TGTCTGTATCCCTTGCCTGGCCGGCACCGGCAAAGCTCAATCTGTTTTTGCACATCAATGGCCGCTTACCCAATGGCTAC
CATGAACTGCAAACCCTGTT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MINLRRFTKFTLAGLTALSLLGGCSVTPKVALQPVSVENASDAKAWELKGKLLIRTNGDKVSANLFWLNTPDNAELRLTS
MLGTTVLLLTQNRDGATLEVDGKRYSDLSPQRLLDGLSGFTLPIDALPFWITGQPMAGDEVEFDTLNRPKTIISADGEWT
INISSWQTQSGAPVPRMLELTHASAVIKLQTNEWQALANATGSKGAR

Sequences:

>Translated_207_residues
MINLRRFTKFTLAGLTALSLLGGCSVTPKVALQPVSVENASDAKAWELKGKLLIRTNGDKVSANLFWLNTPDNAELRLTS
MLGTTVLLLTQNRDGATLEVDGKRYSDLSPQRLLDGLSGFTLPIDALPFWITGQPMAGDEVEFDTLNRPKTIISADGEWT
INISSWQTQSGAPVPRMLELTHASAVIKLQTNEWQALANATGSKGAR
>Mature_207_residues
MINLRRFTKFTLAGLTALSLLGGCSVTPKVALQPVSVENASDAKAWELKGKLLIRTNGDKVSANLFWLNTPDNAELRLTS
MLGTTVLLLTQNRDGATLEVDGKRYSDLSPQRLLDGLSGFTLPIDALPFWITGQPMAGDEVEFDTLNRPKTIISADGEWT
INISSWQTQSGAPVPRMLELTHASAVIKLQTNEWQALANATGSKGAR

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=200, Percent_Identity=28, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_SHEAM (A1S8R4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928440.1
- ProteinModelPortal:   A1S8R4
- SMR:   A1S8R4
- STRING:   A1S8R4
- GeneID:   4604815
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2568
- NMPDR:   fig|326297.7.peg.2408
- eggNOG:   COG3017
- HOGENOM:   HBG677870
- OMA:   PANREIP
- PhylomeDB:   A1S8R4
- ProtClustDB:   CLSK907366
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 22466; Mature: 22466

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLRRFTKFTLAGLTALSLLGGCSVTPKVALQPVSVENASDAKAWELKGKLLIRTNGDK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEECCEEEEEECCCE
VSANLFWLNTPDNAELRLTSMLGTTVLLLTQNRDGATLEVDGKRYSDLSPQRLLDGLSGF
EEEEEEEEECCCCCEEEEEEECCEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHCCCCC
TLPIDALPFWITGQPMAGDEVEFDTLNRPKTIISADGEWTINISSWQTQSGAPVPRMLEL
EEECCCCEEEEECCCCCCCCEEECCCCCCCEEEECCCEEEEEEECCCCCCCCCCCHHEEE
THASAVIKLQTNEWQALANATGSKGAR
ECCEEEEEEECCCCEEECCCCCCCCCC
>Mature Secondary Structure
MINLRRFTKFTLAGLTALSLLGGCSVTPKVALQPVSVENASDAKAWELKGKLLIRTNGDK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEECCEEEEEECCCE
VSANLFWLNTPDNAELRLTSMLGTTVLLLTQNRDGATLEVDGKRYSDLSPQRLLDGLSGF
EEEEEEEEECCCCCEEEEEEECCEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHCCCCC
TLPIDALPFWITGQPMAGDEVEFDTLNRPKTIISADGEWTINISSWQTQSGAPVPRMLEL
EEECCCCEEEEECCCCCCCCEEECCCCCCCEEEECCCEEEEEEECCCCCCCCCCCHHEEE
THASAVIKLQTNEWQALANATGSKGAR
ECCEEEEEEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA