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The map label for this gene is dsbA [H]

Identifier: 119774658

GI number: 119774658

Start: 1850669

End: 1851226

Strand: Direct

Name: dsbA [H]

Synonym: Sama_1521

Alternate gene names: 119774658

Gene position: 1850669-1851226 (Clockwise)

Preceding gene: 119774657

Following gene: 119774659

Centisome position: 42.98

GC content: 52.33

Gene sequence:

>558_bases
ATGTCCTTGTCTGCCATGGCAGCGACCTTTGTTGAAGGTAAGGATTATGTCAGGGTAGAAGGCTTCAGCGAGAGCCATAC
CCCCGTGCTACGGGAGTTTTTTTCCTATAACTGCCCTCATTGTTTTCGCATGGACCACACCATCGAAGAAGCGGTGGGTT
TGGTCGCAAAAGAGGTTCGTTTCGAGCGAACGCCGGTCGGCGCAGGCCGTAACCCCTGGCAAATGAGCCAACTGGCTTAT
TATCTTGCTCAAAAATTCAACGTAACCGACCAGACCCACGGCGCCATTTTCAAGCAGGTTCAAGAAGTCGCTCCATTTCA
GTCTGAAGCTGACGTGCGGCAGTTTTTTGTGTCCCAGGGGCTTAAGGCTGACGAACTGGATAAGGCAATCGCGTCATCCG
ACCGTAAACTGGCCATGATGACTTTTGATACCGAGGCGCAGCTCTCAGGTATCCGTGGTGTGCCAAGCCTGCTGGTGAAT
GGCAAATACATGTTGAACATTCAGGGCCGCAGCAGCGCTGAGATAGCCGAGTTGGTGCAATACCTGGTCAAGCAGTAA

Upstream 100 bases:

>100_bases
GCATGCTGCAGATGCGTTTTAAAGCATAAAATCATTGATAGAAGGAAGTTTGTCCGTGAAAACATCATTAAAGCTGTTGG
GCGCCTTAGGGGGGCTGCTG

Downstream 100 bases:

>100_bases
ACATACATGGTGAAAGAAACGCCCGGCTATGCCGGGCGTTTTACTTTGTTAGACCTAGGTCGGAATGGATGCAATAAATC
TGATTTGAACCATCACAAAG

Product: DsbA family thiol:disulfide interchange protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSLSAMAATFVEGKDYVRVEGFSESHTPVLREFFSYNCPHCFRMDHTIEEAVGLVAKEVRFERTPVGAGRNPWQMSQLAY
YLAQKFNVTDQTHGAIFKQVQEVAPFQSEADVRQFFVSQGLKADELDKAIASSDRKLAMMTFDTEAQLSGIRGVPSLLVN
GKYMLNIQGRSSAEIAELVQYLVKQ

Sequences:

>Translated_185_residues
MSLSAMAATFVEGKDYVRVEGFSESHTPVLREFFSYNCPHCFRMDHTIEEAVGLVAKEVRFERTPVGAGRNPWQMSQLAY
YLAQKFNVTDQTHGAIFKQVQEVAPFQSEADVRQFFVSQGLKADELDKAIASSDRKLAMMTFDTEAQLSGIRGVPSLLVN
GKYMLNIQGRSSAEIAELVQYLVKQ
>Mature_184_residues
SLSAMAATFVEGKDYVRVEGFSESHTPVLREFFSYNCPHCFRMDHTIEEAVGLVAKEVRFERTPVGAGRNPWQMSQLAYY
LAQKFNVTDQTHGAIFKQVQEVAPFQSEADVRQFFVSQGLKADELDKAIASSDRKLAMMTFDTEAQLSGIRGVPSLLVNG
KYMLNIQGRSSAEIAELVQYLVKQ

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=190, Percent_Identity=30, Blast_Score=87, Evalue=6e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 20769; Mature: 20638

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSAMAATFVEGKDYVRVEGFSESHTPVLREFFSYNCPHCFRMDHTIEEAVGLVAKEVR
CCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
FERTPVGAGRNPWQMSQLAYYLAQKFNVTDQTHGAIFKQVQEVAPFQSEADVRQFFVSQG
HHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
LKADELDKAIASSDRKLAMMTFDTEAQLSGIRGVPSLLVNGKYMLNIQGRSSAEIAELVQ
CCHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCHHHHHCCEEEEEECCCCCHHHHHHHH
YLVKQ
HHHCC
>Mature Secondary Structure 
SLSAMAATFVEGKDYVRVEGFSESHTPVLREFFSYNCPHCFRMDHTIEEAVGLVAKEVR
CHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
FERTPVGAGRNPWQMSQLAYYLAQKFNVTDQTHGAIFKQVQEVAPFQSEADVRQFFVSQG
HHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
LKADELDKAIASSDRKLAMMTFDTEAQLSGIRGVPSLLVNGKYMLNIQGRSSAEIAELVQ
CCHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCHHHHHCCEEEEEECCCCCHHHHHHHH
YLVKQ
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA