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The map label for this gene is rlmI [H]

Identifier: 119774628

GI number: 119774628

Start: 1804939

End: 1805865

Strand: Direct

Name: rlmI [H]

Synonym: Sama_1491

Alternate gene names: 119774628

Gene position: 1804939-1805865 (Clockwise)

Preceding gene: 119774627

Following gene: 119774633

Centisome position: 41.92

GC content: 54.58

Gene sequence:

>927_bases
ATGCAGGCTTTTATCGACTTTTTAGCGACGGCCGAATTACCGCAAGACTGCCGACGTCTCTTTCACGGTCGCGGAGGCCG
TTACCCCGGCGCAGAGCACCTGTGTCTTGACTGGTATGCACCGGCGTTGCTGCTGACCTCGTTTTGTGAACTCAGTGCCG
ATGAGCTGGCGACTGCAAAAGCTGCGTTGGAGACGTTTTGGCAGCGTGTAGGCAAAGCTGCTCCCCTGATATTGGTATAT
CAGTATCGCAGTGGCGCCGGTACCCGCACTGAATGCCTCTGCGGTGAGTTGCCAGACAGTCATGTGATTGAGGAGCAGGG
ACTTAAGTTTTTGGTGCACTTGATGCGTGGCCAGAATCACGGGCTATTTCTCGATATGCGCGAAGGTCGCCGCTTTGTAC
ATGAGCATGCCAGGGGAAAGAAGGTACTGAATCTCTTCAGCTACACCTGTGCCTTTTCGGTGGCGGCCCTGGCTGGAGGC
GCGACCGAGGTAGTGAATATCGACATGGCAAAGGGCGCACTTTCCATTGGTAAACAAAACCATGACCTCAATGGTCTGGT
GGGGGGCGCTCGATTTCTCGGCCACGATATCTTCAGTAGCTGGGGTAAGCTGACCCGTTACGGCCCCTACGACCTGGTGA
TTGCCGATCCACCCAGCAACCAAAAAGGCAGTTTTATCGCCACCAAAGATTATGTGCGCTTACTGAGGCGCTTACCTGAG
CTGGTAAAAGAAGGCGGCGACCTGTTGCTATGTCTGAATGCCCCTGAGCTTGGGAGTAATTTTTTGATGTCACAGGTCGA
CGAGACGGCACCCTCCCTGGCGTTTGTAACACGTATCGCCAATCCTGCCGTGTACGCCGACGTGGATGAGGAGAAATCCT
TGAAGGTGCTGCACTATCGAAAGTGTACACCCGCAATCGACGCCTGA

Upstream 100 bases:

>100_bases
TCCGGTTGATAAGGGCTGAACGCCAAACCTGTTTCAGTAATGGGCGGCTTGGCCGCCCATTGTATTTTCAGAAGCCTATT
TTTAGAGGAGCCGGGTTTTC

Downstream 100 bases:

>100_bases
TGCTGGCGATTGACGCCTGCGACTGAGACCTGATGTTTGCTGCGCGAGACTATCAGTATGGGCGGTTCTGCGTGGTGAAA
GCTGTCTAACCAGTAGCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MQAFIDFLATAELPQDCRRLFHGRGGRYPGAEHLCLDWYAPALLLTSFCELSADELATAKAALETFWQRVGKAAPLILVY
QYRSGAGTRTECLCGELPDSHVIEEQGLKFLVHLMRGQNHGLFLDMREGRRFVHEHARGKKVLNLFSYTCAFSVAALAGG
ATEVVNIDMAKGALSIGKQNHDLNGLVGGARFLGHDIFSSWGKLTRYGPYDLVIADPPSNQKGSFIATKDYVRLLRRLPE
LVKEGGDLLLCLNAPELGSNFLMSQVDETAPSLAFVTRIANPAVYADVDEEKSLKVLHYRKCTPAIDA

Sequences:

>Translated_308_residues
MQAFIDFLATAELPQDCRRLFHGRGGRYPGAEHLCLDWYAPALLLTSFCELSADELATAKAALETFWQRVGKAAPLILVY
QYRSGAGTRTECLCGELPDSHVIEEQGLKFLVHLMRGQNHGLFLDMREGRRFVHEHARGKKVLNLFSYTCAFSVAALAGG
ATEVVNIDMAKGALSIGKQNHDLNGLVGGARFLGHDIFSSWGKLTRYGPYDLVIADPPSNQKGSFIATKDYVRLLRRLPE
LVKEGGDLLLCLNAPELGSNFLMSQVDETAPSLAFVTRIANPAVYADVDEEKSLKVLHYRKCTPAIDA
>Mature_308_residues
MQAFIDFLATAELPQDCRRLFHGRGGRYPGAEHLCLDWYAPALLLTSFCELSADELATAKAALETFWQRVGKAAPLILVY
QYRSGAGTRTECLCGELPDSHVIEEQGLKFLVHLMRGQNHGLFLDMREGRRFVHEHARGKKVLNLFSYTCAFSVAALAGG
ATEVVNIDMAKGALSIGKQNHDLNGLVGGARFLGHDIFSSWGKLTRYGPYDLVIADPPSNQKGSFIATKDYVRLLRRLPE
LVKEGGDLLLCLNAPELGSNFLMSQVDETAPSLAFVTRIANPAVYADVDEEKSLKVLHYRKCTPAIDA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=254, Percent_Identity=30.7086614173228, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787181, Length=186, Percent_Identity=32.7956989247312, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 33929; Mature: 33929

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFIDFLATAELPQDCRRLFHGRGGRYPGAEHLCLDWYAPALLLTSFCELSADELATAK
CHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHH
AALETFWQRVGKAAPLILVYQYRSGAGTRTECLCGELPDSHVIEEQGLKFLVHLMRGQNH
HHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
GLFLDMREGRRFVHEHARGKKVLNLFSYTCAFSVAALAGGATEVVNIDMAKGALSIGKQN
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHCCCC
HDLNGLVGGARFLGHDIFSSWGKLTRYGPYDLVIADPPSNQKGSFIATKDYVRLLRRLPE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECHHHHHHHHHHHH
LVKEGGDLLLCLNAPELGSNFLMSQVDETAPSLAFVTRIANPAVYADVDEEKSLKVLHYR
HHHCCCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCEEECCCCCCCEEEEEEE
KCTPAIDA
CCCCCCCC
>Mature Secondary Structure
MQAFIDFLATAELPQDCRRLFHGRGGRYPGAEHLCLDWYAPALLLTSFCELSADELATAK
CHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHH
AALETFWQRVGKAAPLILVYQYRSGAGTRTECLCGELPDSHVIEEQGLKFLVHLMRGQNH
HHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
GLFLDMREGRRFVHEHARGKKVLNLFSYTCAFSVAALAGGATEVVNIDMAKGALSIGKQN
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHCCCC
HDLNGLVGGARFLGHDIFSSWGKLTRYGPYDLVIADPPSNQKGSFIATKDYVRLLRRLPE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECHHHHHHHHHHHH
LVKEGGDLLLCLNAPELGSNFLMSQVDETAPSLAFVTRIANPAVYADVDEEKSLKVLHYR
HHHCCCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCEEECCCCCCCEEEEEEE
KCTPAIDA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA