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The map label for this gene is cobB [H]

Identifier: 119774572

GI number: 119774572

Start: 1745846

End: 1746592

Strand: Direct

Name: cobB [H]

Synonym: Sama_1435

Alternate gene names: 119774572

Gene position: 1745846-1746592 (Clockwise)

Preceding gene: 119774570

Following gene: 119774573

Centisome position: 40.54

GC content: 53.28

Gene sequence:

>747_bases
ATGTACCGGCATATAGTAGTACTCACAGGCGCAGGGATTTCTGCAGAGTCCGGGATCCGTACCTTTCGCGATCAGGATGG
CCTGTGGGAAGAGCACCATATAGAAGACGTGGCGACCCCTGAAGGTTATGAGCGCGATCCTGAACTGGTAGAGCGTTTTT
ACAATCTGCGCTGGGAGCAGCTAAAGTCGACAGACGTTAAAGCTAACCCGGCCCACGAGGCGCTTGCGCAGCTTGAAGCT
GAATTTGACGGCGACTTTTTGCTGGTAACCCAAAACGTGGATGATTTGCACGAGCGCGGTGGCAGCCAACGGCTTATTCA
TATGCATGGCGAGCTCAATAAAGGCCGTTGTCCCCGCTCACGTCAAACTTTCTTATTGCGTGAGCCCTTTGGTCCGGACA
ATGTTTGTACCTGCTGTATCCCTCCCCAGCGTTTGCGGCCCCATATCGTCTGGTTTGGTGAAATGCCTTTTGGCATGGAT
CGCATCCACGATGCGCTGGAACGCTGCGATCTCTTTATTGCCATAGGTACCTCAGGCACTGTGTACCCGGCGGCAGGCTT
TGTGGATATCGCCAATCATCATGGTGCCCAAACAGTGGAAGTGAATCTCGCAGCTCCCGATAGACACAGTCAGTTTCAAT
GGCACCTCACAGGTAAAGCCGGAGACATAGTGCCACGATTGGTGAAGGCTGTGCTTCAAGGAAAAAGCTTGTGCAGCTTG
GCCGATGTGCTGCAAGAGACCGACTGA

Upstream 100 bases:

>100_bases
TCAAAAAGGCACATTCAGATAAGGGTTATACCAAGGCACAATTGTCAGCGACCTGCGTGAAATGGCAGGCTGGTAACAAA
ATAATAAGGAAGGCATCAGC

Downstream 100 bases:

>100_bases
AGAATGAAAAAAGCCATTATTCTCAGAGGCCTGCCCGGCAGCGGGAAGTCATTTTGGGTGACAGAGTTTCTGGCGTCGTT
ACCCGAGGCGGCTCACCCAG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MYRHIVVLTGAGISAESGIRTFRDQDGLWEEHHIEDVATPEGYERDPELVERFYNLRWEQLKSTDVKANPAHEALAQLEA
EFDGDFLLVTQNVDDLHERGGSQRLIHMHGELNKGRCPRSRQTFLLREPFGPDNVCTCCIPPQRLRPHIVWFGEMPFGMD
RIHDALERCDLFIAIGTSGTVYPAAGFVDIANHHGAQTVEVNLAAPDRHSQFQWHLTGKAGDIVPRLVKAVLQGKSLCSL
ADVLQETD

Sequences:

>Translated_248_residues
MYRHIVVLTGAGISAESGIRTFRDQDGLWEEHHIEDVATPEGYERDPELVERFYNLRWEQLKSTDVKANPAHEALAQLEA
EFDGDFLLVTQNVDDLHERGGSQRLIHMHGELNKGRCPRSRQTFLLREPFGPDNVCTCCIPPQRLRPHIVWFGEMPFGMD
RIHDALERCDLFIAIGTSGTVYPAAGFVDIANHHGAQTVEVNLAAPDRHSQFQWHLTGKAGDIVPRLVKAVLQGKSLCSL
ADVLQETD
>Mature_248_residues
MYRHIVVLTGAGISAESGIRTFRDQDGLWEEHHIEDVATPEGYERDPELVERFYNLRWEQLKSTDVKANPAHEALAQLEA
EFDGDFLLVTQNVDDLHERGGSQRLIHMHGELNKGRCPRSRQTFLLREPFGPDNVCTCCIPPQRLRPHIVWFGEMPFGMD
RIHDALERCDLFIAIGTSGTVYPAAGFVDIANHHGAQTVEVNLAAPDRHSQFQWHLTGKAGDIVPRLVKAVLQGKSLCSL
ADVLQETD

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=232, Percent_Identity=34.4827586206897, Blast_Score=141, Evalue=4e-34,
Organism=Homo sapiens, GI6912664, Length=250, Percent_Identity=32.8, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI13787215, Length=238, Percent_Identity=32.7731092436975, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI6912662, Length=261, Percent_Identity=26.8199233716475, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI308199517, Length=237, Percent_Identity=56.5400843881857, Blast_Score=269, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17541892, Length=237, Percent_Identity=28.6919831223629, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137536, Length=229, Percent_Identity=28.3842794759825, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27913; Mature: 27913

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRHIVVLTGAGISAESGIRTFRDQDGLWEEHHIEDVATPEGYERDPELVERFYNLRWEQ
CCEEEEEEECCCCCCCCCHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHH
LKSTDVKANPAHEALAQLEAEFDGDFLLVTQNVDDLHERGGSQRLIHMHGELNKGRCPRS
HHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCCC
RQTFLLREPFGPDNVCTCCIPPQRLRPHIVWFGEMPFGMDRIHDALERCDLFIAIGTSGT
CCEEEEECCCCCCCEEEECCCHHHCCCEEEEEECCCCCHHHHHHHHHHCEEEEEECCCCC
VYPAAGFVDIANHHGAQTVEVNLAAPDRHSQFQWHLTGKAGDIVPRLVKAVLQGKSLCSL
EECCCCEEECCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHH
ADVLQETD
HHHHHCCC
>Mature Secondary Structure
MYRHIVVLTGAGISAESGIRTFRDQDGLWEEHHIEDVATPEGYERDPELVERFYNLRWEQ
CCEEEEEEECCCCCCCCCHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHH
LKSTDVKANPAHEALAQLEAEFDGDFLLVTQNVDDLHERGGSQRLIHMHGELNKGRCPRS
HHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCCC
RQTFLLREPFGPDNVCTCCIPPQRLRPHIVWFGEMPFGMDRIHDALERCDLFIAIGTSGT
CCEEEEECCCCCCCEEEECCCHHHCCCEEEEEECCCCCHHHHHHHHHHCEEEEEECCCCC
VYPAAGFVDIANHHGAQTVEVNLAAPDRHSQFQWHLTGKAGDIVPRLVKAVLQGKSLCSL
EECCCCEEECCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHH
ADVLQETD
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]