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The map label for this gene is iscA [H]

Identifier: 119774432

GI number: 119774432

Start: 1581544

End: 1581867

Strand: Direct

Name: iscA [H]

Synonym: Sama_1295

Alternate gene names: 119774432

Gene position: 1581544-1581867 (Clockwise)

Preceding gene: 119774431

Following gene: 119774433

Centisome position: 36.73

GC content: 50.93

Gene sequence:

>324_bases
ATGGCGATTTCTATGACACCCGCAGCCAATGAGCGAGTCAAAGCCTTCCTTGCCAATCGCGGTAAGGGTGTAGGCCTTCG
TCTCGGTCTCAAGACGTCTGGCTGCTCGGGTATGGCCTACGTGCTGGAATTTGTTGACGATCTCAACGATGACGACGAAG
TCTACGAAATAGACGGTGTTAATATCATCATCGATGCCAAGAGCTTCATTTACCTGCAGGGTATCGAGCTCGATTTCGTC
AAGGAAGGCCTCAATGAGGGCTTCAAATTCAATAACCCCAATGCCAAGGGAGAATGCGGCTGCGGCGAGAGCTTCACCGT
ATAA

Upstream 100 bases:

>100_bases
GCCACCGGTGAAGATCCACTGTTCTATTCTGGCCGAAGATGCCATTAAGGCAGCTCTGGAAGAGTACAAGCACAAGCAAG
CCAAGTAATTCGGAGTTCGT

Downstream 100 bases:

>100_bases
TCCGGTTTTATGAATTACTTCGAGCTGTTTAACCTTCCGGTTGCTTTCGATGTGAATACCAGTGAGCTCGCGGACAAATA
CCGCGAGCTTCAGCGTACCG

Product: HesB/YadR/YfhF family protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAISMTPAANERVKAFLANRGKGVGLRLGLKTSGCSGMAYVLEFVDDLNDDDEVYEIDGVNIIIDAKSFIYLQGIELDFV
KEGLNEGFKFNNPNAKGECGCGESFTV

Sequences:

>Translated_107_residues
MAISMTPAANERVKAFLANRGKGVGLRLGLKTSGCSGMAYVLEFVDDLNDDDEVYEIDGVNIIIDAKSFIYLQGIELDFV
KEGLNEGFKFNNPNAKGECGCGESFTV
>Mature_106_residues
AISMTPAANERVKAFLANRGKGVGLRLGLKTSGCSGMAYVLEFVDDLNDDDEVYEIDGVNIIIDAKSFIYLQGIELDFVK
EGLNEGFKFNNPNAKGECGCGESFTV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=42.4528301886792, Blast_Score=105, Evalue=8e-24,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=36.144578313253, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=65.4205607476635, Blast_Score=149, Evalue=5e-38,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=44.7619047619048, Blast_Score=108, Evalue=8e-26,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=39.0909090909091, Blast_Score=72, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=39.622641509434, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=39.622641509434, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=34.9056603773585, Blast_Score=61, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=40.9523809523809, Blast_Score=93, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=44.3396226415094, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11577; Mature: 11446

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISMTPAANERVKAFLANRGKGVGLRLGLKTSGCSGMAYVLEFVDDLNDDDEVYEIDGV
CEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCC
NIIIDAKSFIYLQGIELDFVKEGLNEGFKFNNPNAKGECGCGESFTV
EEEEECCCEEEEECCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AISMTPAANERVKAFLANRGKGVGLRLGLKTSGCSGMAYVLEFVDDLNDDDEVYEIDGV
EEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCC
NIIIDAKSFIYLQGIELDFVKEGLNEGFKFNNPNAKGECGCGESFTV
EEEEECCCEEEEECCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA