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The map label for this gene is sbcD [H]

Identifier: 119774404

GI number: 119774404

Start: 1553022

End: 1554167

Strand: Direct

Name: sbcD [H]

Synonym: Sama_1267

Alternate gene names: 119774404

Gene position: 1553022-1554167 (Clockwise)

Preceding gene: 119774403

Following gene: 119774405

Centisome position: 36.07

GC content: 55.76

Gene sequence:

>1146_bases
ATGAGATTCATCCATACCTCAGACTGGCATCTGGGCCGCACACTGCACAATCAGTCGTTACTGGACGAGCAGGCGCAGAT
GCTGGAGACCCTGCTGACACTCATCGAGACCCACGAGGTGGATGCACTCGTGATAGCAGGCGATATCTTCGACCGCTCCG
TGCCACCGGCCGCCGCAGTGAGTCTTCTGGATGATTTTCTCGATAAGGTTGTCCAGCAGCTCAAGGTGAGCGTCATTGCT
ATCGGCGGTAATCACGATGGCCAGGAGCGATTGGCCTTTGGAGCAAGGCAAATGGCCGGTGCCGGCCTTTATATTCAGGG
GCCGGTCAGGGCCAGTATTGAACCCGTCCACATTGATGGGAAGCAAGGCGCTGCATTTTTCTACCCCATCCCTTATGCGG
AGCCTGCGCTCGTTCGCCATCTGCTGAATGATGAGGATATTCACAGCCATCAGGATGCCATGGCGGCCTTGCTGGCAAAG
GTTACGGCCCACAACAGTGACGGGCTGCCCAAGATAGTCATAAGTCACTGCTTTTTAGACGGTGGCAACGAGTCTGATTC
AGAGCGCCCCCTGTCAATCGGCGGCGCCGACAAGATTTCACCGGCGCTGTTCGCACCATTCGATTACGCTGCCCTTGGGC
ATCTGCATGGCCCACAATATAAGGGCGCAGAGCATGTGCGCTACAGCGGCTCTCCCTTGAAATACTCCTTCAGCGAGCAG
CACCAAAATAAGTCGGTCACCCTGGTGAATATATTGCCCGGCCAGGCACCGGATATCCGTTTGCTGCCCATCAAACCCCA
GCGCGATGTGCGCATTATCGAAGGCTATCTGGATGCGCTCCTTACACGGGGAGCAAGCGACCCCGGCCGGGATGATTACC
TGATGGTGCGATTGCTCGATACCCATGCCATTTTAGAACCCATGGCAAAGCTTCGGGCAATCTACCCGAATGTGCTGCAC
CTTGAACGTACCGGGCTGATGCAGGAAAGGGCACTGGAGGGGCCTGGCCGAGAGCGCATGCAAAAAAGCGAACTGACCAT
GTTTGAAGATTTCTTTACTCAGGTTCAGGGGGAACCCCTTTCCGGGCCACAAAAAACATTGATGTCCGAATTGATTGCCT
CCTTGCACAGGGAGGGCGGTCAATGA

Upstream 100 bases:

>100_bases
ATTGGCTGAGCGCCCGGTGCTGTGGAGGTGGTTTGCGCCTTCACAGCGTCTTTGATATCCCCTAGTATGAGTCTCAATTT
TAAAGCAAAAAGGCTTTTGC

Downstream 100 bases:

>100_bases
AGCCCTTAACCCTTGCCATCACCGCCTTTGGTCCCTTCGTGGACACCCAAACCCTGGACTTCCGCGCCCTTGGCGAGTGG
CCCTTGTTCCTGATTAACGG

Product: exonuclease SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRFIHTSDWHLGRTLHNQSLLDEQAQMLETLLTLIETHEVDALVIAGDIFDRSVPPAAAVSLLDDFLDKVVQQLKVSVIA
IGGNHDGQERLAFGARQMAGAGLYIQGPVRASIEPVHIDGKQGAAFFYPIPYAEPALVRHLLNDEDIHSHQDAMAALLAK
VTAHNSDGLPKIVISHCFLDGGNESDSERPLSIGGADKISPALFAPFDYAALGHLHGPQYKGAEHVRYSGSPLKYSFSEQ
HQNKSVTLVNILPGQAPDIRLLPIKPQRDVRIIEGYLDALLTRGASDPGRDDYLMVRLLDTHAILEPMAKLRAIYPNVLH
LERTGLMQERALEGPGRERMQKSELTMFEDFFTQVQGEPLSGPQKTLMSELIASLHREGGQ

Sequences:

>Translated_381_residues
MRFIHTSDWHLGRTLHNQSLLDEQAQMLETLLTLIETHEVDALVIAGDIFDRSVPPAAAVSLLDDFLDKVVQQLKVSVIA
IGGNHDGQERLAFGARQMAGAGLYIQGPVRASIEPVHIDGKQGAAFFYPIPYAEPALVRHLLNDEDIHSHQDAMAALLAK
VTAHNSDGLPKIVISHCFLDGGNESDSERPLSIGGADKISPALFAPFDYAALGHLHGPQYKGAEHVRYSGSPLKYSFSEQ
HQNKSVTLVNILPGQAPDIRLLPIKPQRDVRIIEGYLDALLTRGASDPGRDDYLMVRLLDTHAILEPMAKLRAIYPNVLH
LERTGLMQERALEGPGRERMQKSELTMFEDFFTQVQGEPLSGPQKTLMSELIASLHREGGQ
>Mature_381_residues
MRFIHTSDWHLGRTLHNQSLLDEQAQMLETLLTLIETHEVDALVIAGDIFDRSVPPAAAVSLLDDFLDKVVQQLKVSVIA
IGGNHDGQERLAFGARQMAGAGLYIQGPVRASIEPVHIDGKQGAAFFYPIPYAEPALVRHLLNDEDIHSHQDAMAALLAK
VTAHNSDGLPKIVISHCFLDGGNESDSERPLSIGGADKISPALFAPFDYAALGHLHGPQYKGAEHVRYSGSPLKYSFSEQ
HQNKSVTLVNILPGQAPDIRLLPIKPQRDVRIIEGYLDALLTRGASDPGRDDYLMVRLLDTHAILEPMAKLRAIYPNVLH
LERTGLMQERALEGPGRERMQKSELTMFEDFFTQVQGEPLSGPQKTLMSELIASLHREGGQ

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=411, Percent_Identity=30.4136253041363, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 41969; Mature: 41969

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIHTSDWHLGRTLHNQSLLDEQAQMLETLLTLIETHEVDALVIAGDIFDRSVPPAAAV
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHHHH
SLLDDFLDKVVQQLKVSVIAIGGNHDGQERLAFGARQMAGAGLYIQGPVRASIEPVHIDG
HHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECC
KQGAAFFYPIPYAEPALVRHLLNDEDIHSHQDAMAALLAKVTAHNSDGLPKIVISHCFLD
CCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
GGNESDSERPLSIGGADKISPALFAPFDYAALGHLHGPQYKGAEHVRYSGSPLKYSFSEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCEECHHHH
HQNKSVTLVNILPGQAPDIRLLPIKPQRDVRIIEGYLDALLTRGASDPGRDDYLMVRLLD
CCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHH
THAILEPMAKLRAIYPNVLHLERTGLMQERALEGPGRERMQKSELTMFEDFFTQVQGEPL
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SGPQKTLMSELIASLHREGGQ
CCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFIHTSDWHLGRTLHNQSLLDEQAQMLETLLTLIETHEVDALVIAGDIFDRSVPPAAAV
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHHHH
SLLDDFLDKVVQQLKVSVIAIGGNHDGQERLAFGARQMAGAGLYIQGPVRASIEPVHIDG
HHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECC
KQGAAFFYPIPYAEPALVRHLLNDEDIHSHQDAMAALLAKVTAHNSDGLPKIVISHCFLD
CCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
GGNESDSERPLSIGGADKISPALFAPFDYAALGHLHGPQYKGAEHVRYSGSPLKYSFSEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCEECHHHH
HQNKSVTLVNILPGQAPDIRLLPIKPQRDVRIIEGYLDALLTRGASDPGRDDYLMVRLLD
CCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHH
THAILEPMAKLRAIYPNVLHLERTGLMQERALEGPGRERMQKSELTMFEDFFTQVQGEPL
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SGPQKTLMSELIASLHREGGQ
CCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA