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The map label for this gene is def2 [H]

Identifier: 119773777

GI number: 119773777

Start: 795431

End: 795955

Strand: Reverse

Name: def2 [H]

Synonym: Sama_0639

Alternate gene names: 119773777

Gene position: 795955-795431 (Counterclockwise)

Preceding gene: 119773778

Following gene: 119773776

Centisome position: 18.48

GC content: 57.14

Gene sequence:

>525_bases
ATGGCCAGCATCCAAACCGCCCCCATCGCCATCACAGGTGACCCGGTGCTCTACCAAAAAGCCGCACCTGTCACTGTATT
TGATGACAGTCTGGAGAGGCTTTCGCAAACCCTGATGGCCACCATGTTGCAGGCCAAAGGCGTGGGTATAGCCGCGCCTC
AGATTGGTGTGAGCCAGCGGCTTTTCATCGTCGCATCCCGCCCCAATGAGCGTTATCCCGATGCGCCCCTGATGGAGCCC
ATGGTGATGGTCAATCCTGCGCTGCTGTCGGGATCTTCAGAATGGGAATCGAGTGAAGAAGGCTGTCTGTCGGTTCCGGG
AAAAAGGCTGTCTATCGCTCGCCATCGCTGGGTCGAAGCCAAGTGGCAGGACTTACAGGGCAATGCTTACTCCGGTCGTC
TTGATGGTTTTATTGCCCGAATCTTTCAGCATGAGCTGGACCATCTCGATGGCATCACCCTGTTGGAACGCGTCACCATG
GCCGCACAAGCGCACACGATGAACGCGGGAGAGACGCCACTGTGA

Upstream 100 bases:

>100_bases
GAGTAAGCTAAAGGCGATGGAACCGAGCAATATCGCGACCCAGGCGGAAGAAACACCGCCGCCGCACTACTAATAATCCG
CCGCAGGAACAGGGAATTTC

Downstream 100 bases:

>100_bases
GTCAACGGGCCCCAGTACTCATGACAGCGCTCTTCCTTGGCGGCTGCGCCATGGGCGGCTTCTTTATGAGTTATCCGGCG
CAGATGGCACAGGTAAAGCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MASIQTAPIAITGDPVLYQKAAPVTVFDDSLERLSQTLMATMLQAKGVGIAAPQIGVSQRLFIVASRPNERYPDAPLMEP
MVMVNPALLSGSSEWESSEEGCLSVPGKRLSIARHRWVEAKWQDLQGNAYSGRLDGFIARIFQHELDHLDGITLLERVTM
AAQAHTMNAGETPL

Sequences:

>Translated_174_residues
MASIQTAPIAITGDPVLYQKAAPVTVFDDSLERLSQTLMATMLQAKGVGIAAPQIGVSQRLFIVASRPNERYPDAPLMEP
MVMVNPALLSGSSEWESSEEGCLSVPGKRLSIARHRWVEAKWQDLQGNAYSGRLDGFIARIFQHELDHLDGITLLERVTM
AAQAHTMNAGETPL
>Mature_173_residues
ASIQTAPIAITGDPVLYQKAAPVTVFDDSLERLSQTLMATMLQAKGVGIAAPQIGVSQRLFIVASRPNERYPDAPLMEPM
VMVNPALLSGSSEWESSEEGCLSVPGKRLSIARHRWVEAKWQDLQGNAYSGRLDGFIARIFQHELDHLDGITLLERVTMA
AQAHTMNAGETPL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=163, Percent_Identity=33.1288343558282, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789682, Length=138, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24645728, Length=162, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24645726, Length=154, Percent_Identity=33.7662337662338, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18985; Mature: 18854

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIQTAPIAITGDPVLYQKAAPVTVFDDSLERLSQTLMATMLQAKGVGIAAPQIGVSQR
CCCCCCCCEEEECCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCE
LFIVASRPNERYPDAPLMEPMVMVNPALLSGSSEWESSEEGCLSVPGKRLSIARHRWVEA
EEEEECCCCCCCCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHH
KWQDLQGNAYSGRLDGFIARIFQHELDHLDGITLLERVTMAAQAHTMNAGETPL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ASIQTAPIAITGDPVLYQKAAPVTVFDDSLERLSQTLMATMLQAKGVGIAAPQIGVSQR
CCCCCCCEEEECCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCE
LFIVASRPNERYPDAPLMEPMVMVNPALLSGSSEWESSEEGCLSVPGKRLSIARHRWVEA
EEEEECCCCCCCCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHH
KWQDLQGNAYSGRLDGFIARIFQHELDHLDGITLLERVTMAAQAHTMNAGETPL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]