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The map label for this gene is rsmE [H]

Identifier: 119773713

GI number: 119773713

Start: 716297

End: 717028

Strand: Reverse

Name: rsmE [H]

Synonym: Sama_0575

Alternate gene names: 119773713

Gene position: 717028-716297 (Counterclockwise)

Preceding gene: 119773714

Following gene: 119773712

Centisome position: 16.65

GC content: 53.01

Gene sequence:

>732_bases
ATGCGAATTCCAAGAATTTTCCAGCCACTTGACCTGTCTCCCTCAGCGGAGTTTGCCCTCGATGAAGATGGCTTTGCCCA
CGTCGGCCGGGTGCTTCGCATGGGTGAAGGCGAACAGATCAGGGTATTCAATGGCGATGGTAAAGATTACCTGGCTGACA
TAGTCTCGGCAGGTAAAAAGTCACTGACGGTGAAGGTTGTTGCGGTAGAAGACAACCAGAGTGAATCGCCGCTGCATTTG
CATCTGGGCCAGGTAATCTCCCGTGGTGACCGTATGGATTTCACCCTGCAAAAATCAGTCGAACTCGGTGTGAACACCAT
CACGCCGCTTTTTTCAGAACGCTGCGGCGTCAAGCTCAGCGGTGAGCGGCTCGATAAAAAAATTGCCCAATGGCAAAAAA
TCGTGATCAGCGCCTGTGAACAATGTGGCCGGGCCACAGTACCCGTTGTTCGCCCTGTAATGGATCTGGCCGAGTGGTGC
GCCGAACAAACCAGCGCGCTCAAGTTAAATCTTCATCCCCGTGCTGACCATGGTATCAATGGTCTGGTGCTGGATAATCC
CAGAGTAAGGCTGCTTATTGGCCCCGAGGGAGGGCTGTCGGCAGAAGAGATTGCCATGACAGAAACCCACAAATTTACCG
AAGTGTTGCTCGGTCCCCGGGTACTTCGTACTGAAACGGCCGCGCTAACCGCCATTACGGCACTGCAAATGAAATTTGGC
GATCTGGGTTAA

Upstream 100 bases:

>100_bases
TTGCCGGCGCCCAGGGTAACCACAATCCCTTTGTTGCACGCGGCTGTAAAGCGGGTGCACTTGATGTTCAAACGGCAGAT
TAACAGTTTCAGGATCCCGA

Downstream 100 bases:

>100_bases
ACCCGGCTCCCCCGCATGAGAGGACGCAATATGATTAAACTCGGCATAGTGATGGACCCCATCAGCGACATCAACATCAA
GAAAGACTCCAGCTTTGCCA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRIPRIFQPLDLSPSAEFALDEDGFAHVGRVLRMGEGEQIRVFNGDGKDYLADIVSAGKKSLTVKVVAVEDNQSESPLHL
HLGQVISRGDRMDFTLQKSVELGVNTITPLFSERCGVKLSGERLDKKIAQWQKIVISACEQCGRATVPVVRPVMDLAEWC
AEQTSALKLNLHPRADHGINGLVLDNPRVRLLIGPEGGLSAEEIAMTETHKFTEVLLGPRVLRTETAALTAITALQMKFG
DLG

Sequences:

>Translated_243_residues
MRIPRIFQPLDLSPSAEFALDEDGFAHVGRVLRMGEGEQIRVFNGDGKDYLADIVSAGKKSLTVKVVAVEDNQSESPLHL
HLGQVISRGDRMDFTLQKSVELGVNTITPLFSERCGVKLSGERLDKKIAQWQKIVISACEQCGRATVPVVRPVMDLAEWC
AEQTSALKLNLHPRADHGINGLVLDNPRVRLLIGPEGGLSAEEIAMTETHKFTEVLLGPRVLRTETAALTAITALQMKFG
DLG
>Mature_243_residues
MRIPRIFQPLDLSPSAEFALDEDGFAHVGRVLRMGEGEQIRVFNGDGKDYLADIVSAGKKSLTVKVVAVEDNQSESPLHL
HLGQVISRGDRMDFTLQKSVELGVNTITPLFSERCGVKLSGERLDKKIAQWQKIVISACEQCGRATVPVVRPVMDLAEWC
AEQTSALKLNLHPRADHGINGLVLDNPRVRLLIGPEGGLSAEEIAMTETHKFTEVLLGPRVLRTETAALTAITALQMKFG
DLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=65.0205761316872, Blast_Score=330, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26590; Mature: 26590

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIFQPLDLSPSAEFALDEDGFAHVGRVLRMGEGEQIRVFNGDGKDYLADIVSAGKK
CCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCC
SLTVKVVAVEDNQSESPLHLHLGQVISRGDRMDFTLQKSVELGVNTITPLFSERCGVKLS
EEEEEEEEEECCCCCCCCEEHHHHHHCCCCCCCEEEHHHHHCCCHHHHHHHHHHCCCEEC
GERLDKKIAQWQKIVISACEQCGRATVPVVRPVMDLAEWCAEQTSALKLNLHPRADHGIN
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
GLVLDNPRVRLLIGPEGGLSAEEIAMTETHKFTEVLLGPRVLRTETAALTAITALQMKFG
EEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
DLG
CCC
>Mature Secondary Structure
MRIPRIFQPLDLSPSAEFALDEDGFAHVGRVLRMGEGEQIRVFNGDGKDYLADIVSAGKK
CCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCC
SLTVKVVAVEDNQSESPLHLHLGQVISRGDRMDFTLQKSVELGVNTITPLFSERCGVKLS
EEEEEEEEEECCCCCCCCEEHHHHHHCCCCCCCEEEHHHHHCCCHHHHHHHHHHCCCEEC
GERLDKKIAQWQKIVISACEQCGRATVPVVRPVMDLAEWCAEQTSALKLNLHPRADHGIN
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
GLVLDNPRVRLLIGPEGGLSAEEIAMTETHKFTEVLLGPRVLRTETAALTAITALQMKFG
EEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
DLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]