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The map label for this gene is maeB [H]

Identifier: 119773588

GI number: 119773588

Start: 553592

End: 554839

Strand: Direct

Name: maeB [H]

Synonym: Sama_0448

Alternate gene names: 119773588

Gene position: 553592-554839 (Clockwise)

Preceding gene: 119773587

Following gene: 119773589

Centisome position: 12.86

GC content: 54.97

Gene sequence:

>1248_bases
ATGGCAGACCTTCGTCAACAAGCACTCGATTATCATGAATTTCCGGTTCCTGGAAAAATGGCTGTGTGTCTCACCAAACC
CGCTGAAACCAGTATGGATCTGGCGCTGGCTTACAGCCCGGGTGTGGCCGAGCCGGTGCGTGAAATCGCCTCTAATCCTG
CCGACGCATACCGCTACACCGCCAAGGGCAACACGGTTGCCGTGATTTCCAACGGTACCGCCATTTTGGGTCTGGGAAAC
CTGGGACCATTGGCCTCCAAGCCTGTAATGGAAGGTAAGGCATTGCTGTTCAAGCATTTTGCCAATATCGATGCCACCGA
TATTCAGGTGAAGCATCGCACGACAGAAGAGTTTATCAATACCGTTGAAGCCATTGCCGACACCTTCGGTGGCATCAATC
TGGAAGACATCAAGGCACCCGAGTGTTTCGAGATTGAGCGTGAGCTGATTGAGCGCTGCGCCATTCCGGTATTCCACGAT
GATCAGCACGGTACCGCCATAGTCACTGCTGCAGGTATGATTAATGCGCTGGAAATTCAGGGTAAATCCATCGATAAAGC
CGTGTTCGTGTGTATGGGGGCCGGCGCCGCCGCCATCGCCTGTATGACCATGCTGGTCAAATGTGGTGCCCAGCGTGAAA
ATGTGTACATGCTGGATCGCAAAGGTGTCATCCACACCCGCCGTGATGACCTGAATGAATACAAGGCTCTGTTTGCCAAC
AACACCGACAAGCGCACTCTGCAGGATGTGATCAAGGGCGCCGATGTGTTCCTGGGTCTGTCCGGTCCGGATGTACTGGG
TGCCGACGATGTGCTCTTGATGGCCGATAATCCGGTGATCTTTGCCTGTTCCAACCCGGATCCTGAGATCAAGCCTGAAC
TCGCCCACGATATCCGTAAAGACATCATCATGGGTACCGGCCGCAGCGATTACCCCAACCAGGTAAACAATGTGCTGTGC
TTCCCCTTCATCTTCCGCGGTGCGCTGGATGTTCGCGCTTCACGCATCAACGATGAGATGAAGATTGCCGCCGTGCATGC
CCTGGCCGATTTGGCGAAAGAGCCTGTCCCTGCCTCTGTGCTGGCTGCCTATCCCAATGTCAGCAGCCTGAGTTTTGGCC
CTGAGTATGTGCTGCCAAAGCCCATGGATCCACGTTTGCTGCCAAGGGTTGCCCGTGCTGTTGCCCAGGCTGCCATCGAT
TCCGGTGTTGCTGCCATCGACCCTCTGCCAGAGAACTACCTCGCCTGA

Upstream 100 bases:

>100_bases
CGCGTAATCTTTATGGCCTTAGTAAAGGGGCCCGAATTTGCTGTTGCGATGCCCTTTTGCCCGCGGATTTACCCATAAAA
ACGTCGTTCAGGATTAAAAA

Downstream 100 bases:

>100_bases
TCTAAATCCCTTAGTGGTAAAGACAGACAAAAGGCAACCCTTGTGGTTGCCTTTTGTTATTGAGTACCTGATAATTTTTA
ACAATTTGCAGTGTCCCGCC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MADLRQQALDYHEFPVPGKMAVCLTKPAETSMDLALAYSPGVAEPVREIASNPADAYRYTAKGNTVAVISNGTAILGLGN
LGPLASKPVMEGKALLFKHFANIDATDIQVKHRTTEEFINTVEAIADTFGGINLEDIKAPECFEIERELIERCAIPVFHD
DQHGTAIVTAAGMINALEIQGKSIDKAVFVCMGAGAAAIACMTMLVKCGAQRENVYMLDRKGVIHTRRDDLNEYKALFAN
NTDKRTLQDVIKGADVFLGLSGPDVLGADDVLLMADNPVIFACSNPDPEIKPELAHDIRKDIIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASRINDEMKIAAVHALADLAKEPVPASVLAAYPNVSSLSFGPEYVLPKPMDPRLLPRVARAVAQAAID
SGVAAIDPLPENYLA

Sequences:

>Translated_415_residues
MADLRQQALDYHEFPVPGKMAVCLTKPAETSMDLALAYSPGVAEPVREIASNPADAYRYTAKGNTVAVISNGTAILGLGN
LGPLASKPVMEGKALLFKHFANIDATDIQVKHRTTEEFINTVEAIADTFGGINLEDIKAPECFEIERELIERCAIPVFHD
DQHGTAIVTAAGMINALEIQGKSIDKAVFVCMGAGAAAIACMTMLVKCGAQRENVYMLDRKGVIHTRRDDLNEYKALFAN
NTDKRTLQDVIKGADVFLGLSGPDVLGADDVLLMADNPVIFACSNPDPEIKPELAHDIRKDIIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASRINDEMKIAAVHALADLAKEPVPASVLAAYPNVSSLSFGPEYVLPKPMDPRLLPRVARAVAQAAID
SGVAAIDPLPENYLA
>Mature_414_residues
ADLRQQALDYHEFPVPGKMAVCLTKPAETSMDLALAYSPGVAEPVREIASNPADAYRYTAKGNTVAVISNGTAILGLGNL
GPLASKPVMEGKALLFKHFANIDATDIQVKHRTTEEFINTVEAIADTFGGINLEDIKAPECFEIERELIERCAIPVFHDD
QHGTAIVTAAGMINALEIQGKSIDKAVFVCMGAGAAAIACMTMLVKCGAQRENVYMLDRKGVIHTRRDDLNEYKALFANN
TDKRTLQDVIKGADVFLGLSGPDVLGADDVLLMADNPVIFACSNPDPEIKPELAHDIRKDIIMGTGRSDYPNQVNNVLCF
PFIFRGALDVRASRINDEMKIAAVHALADLAKEPVPASVLAAYPNVSSLSFGPEYVLPKPMDPRLLPRVARAVAQAAIDS
GVAAIDPLPENYLA

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=353, Percent_Identity=26.628895184136, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI62420880, Length=353, Percent_Identity=26.628895184136, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI239049447, Length=353, Percent_Identity=26.628895184136, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI4505143, Length=344, Percent_Identity=24.7093023255814, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788806, Length=404, Percent_Identity=60.6435643564356, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI87081919, Length=295, Percent_Identity=26.1016949152542, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537199, Length=366, Percent_Identity=26.2295081967213, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356279, Length=411, Percent_Identity=27.7372262773723, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281362674, Length=411, Percent_Identity=27.7372262773723, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281362672, Length=411, Percent_Identity=27.7372262773723, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78707242, Length=402, Percent_Identity=25.6218905472637, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI78707238, Length=402, Percent_Identity=25.6218905472637, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI78707240, Length=341, Percent_Identity=26.6862170087977, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24646388, Length=410, Percent_Identity=27.5609756097561, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24646386, Length=410, Percent_Identity=27.5609756097561, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363503, Length=352, Percent_Identity=24.4318181818182, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363505, Length=352, Percent_Identity=24.4318181818182, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707236, Length=352, Percent_Identity=24.4318181818182, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707232, Length=352, Percent_Identity=24.4318181818182, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 44699; Mature: 44568

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLRQQALDYHEFPVPGKMAVCLTKPAETSMDLALAYSPGVAEPVREIASNPADAYRYT
CCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCHHEEEE
AKGNTVAVISNGTAILGLGNLGPLASKPVMEGKALLFKHFANIDATDIQVKHRTTEEFIN
ECCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
TVEAIADTFGGINLEDIKAPECFEIERELIERCAIPVFHDDQHGTAIVTAAGMINALEIQ
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCEECCCCCCEEEEECCCEEEEEEC
GKSIDKAVFVCMGAGAAAIACMTMLVKCGAQRENVYMLDRKGVIHTRRDDLNEYKALFAN
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCHHHHHHHHHHHCC
NTDKRTLQDVIKGADVFLGLSGPDVLGADDVLLMADNPVIFACSNPDPEIKPELAHDIRK
CCCHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHH
DIIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASRINDEMKIAAVHALADLAKEPVPASV
HHEECCCCCCCCHHHHCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
LAAYPNVSSLSFGPEYVLPKPMDPRLLPRVARAVAQAAIDSGVAAIDPLPENYLA
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCHHCCC
>Mature Secondary Structure 
ADLRQQALDYHEFPVPGKMAVCLTKPAETSMDLALAYSPGVAEPVREIASNPADAYRYT
CCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCHHEEEE
AKGNTVAVISNGTAILGLGNLGPLASKPVMEGKALLFKHFANIDATDIQVKHRTTEEFIN
ECCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
TVEAIADTFGGINLEDIKAPECFEIERELIERCAIPVFHDDQHGTAIVTAAGMINALEIQ
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCEECCCCCCEEEEECCCEEEEEEC
GKSIDKAVFVCMGAGAAAIACMTMLVKCGAQRENVYMLDRKGVIHTRRDDLNEYKALFAN
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCHHHHHHHHHHHCC
NTDKRTLQDVIKGADVFLGLSGPDVLGADDVLLMADNPVIFACSNPDPEIKPELAHDIRK
CCCHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHH
DIIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASRINDEMKIAAVHALADLAKEPVPASV
HHEECCCCCCCCHHHHCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
LAAYPNVSSLSFGPEYVLPKPMDPRLLPRVARAVAQAAIDSGVAAIDPLPENYLA
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]