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The map label for this gene is acnB [H]

Identifier: 119773523

GI number: 119773523

Start: 470282

End: 472879

Strand: Direct

Name: acnB [H]

Synonym: Sama_0382

Alternate gene names: 119773523

Gene position: 470282-472879 (Clockwise)

Preceding gene: 119773521

Following gene: 119773528

Centisome position: 10.92

GC content: 56.54

Gene sequence:

>2598_bases
GTGCTAGAAGCATATCGTAAACACGTCGAAGAACGTGCTGCAGAGGGCGTTGTCCCTAAGCCACTCGATGCCCATCAGGT
AGCCGAACTGGTTGAATTAGTAAAGAATCCTCCAGCTGGTGAAGAAGCCTTCATCCTGGACCTGCTTGAAAACCGTATTC
CCCCCGGCGTTGACGAAGCCGCCTATGTAAAGGCCGGTTTCCTGGATGCCATTGCCAAGGGCACAGCCACTTCTCCCATC
CTGAACGCCGAGCGTGCCGTTGAGCTGCTGGGCACCATGCAGGGTGGCTACAACATCGAGCCGCTGATCCATCAACTGGA
CAACGACAAGCTGGCCCCTCTGGCCGTTAAAGCCCTGTCACACACCCTGCTGATGTTCGACTCTTTCCACGATGTGGTAG
AGAAGATGAATGCCGGCAATGCCCACGCCAAGCAAGTGGTTGAATCCTGGGCCAACGCCGAGTGGTTCCTGAGCCGCCCC
AAGCTGGCTGACAAGATTTCTCTGACCGTATTCAAGGTAACCGGTGAAACCAACACCGACGACCTGTCTCCGGCCCCGGA
TGCCTGGTCTCGTCCAGATATCCCGCTGCACGCCCTGGCGATGCTGAAAAACGCCCGTGACGGCATTGTGCCCGATGAGC
CAGGTGTAGTTGGCCCAATCAAGAAAATTGAAGAGCTGAAAACCCAGGGTTTCCCACTGGTTTACGTGGGTGACGTAGTA
GGTACAGGTTCTTCCCGTAAGTCTGCCACCAACTCTGTGCTGTGGTTCATGGGTGATGACATCCCATTCGTGCCTAACAA
GCGTGCCGGTGGTTTCTGTCTGGGCGGCAAGATTGCCCCTATCTTCTTCAACACCATGGAAGATGCCGGCGCGCTGCCAA
TCGAGCTGGACGTCAGCAAGATGGAAATGGGCGATGTGATTGATATCTACCCTTACGAGGGCAAGGTACGTCGCCATGGC
AGTGATGAAGTGATTTCTGAGTTTGAACTCAAGACCGACGTGCTGCTCGATGAAGTTCGCGCCGGTGGCCGTATTCCGCT
AATCATTGGCCGCGGTCTGACCGACAAGGCCCGTGAAGTGCTGGGTCTGGGCGCGTCGAAAGAGTTCGTTCGTCCGCAGG
ATGTGGCCGACACTGGCAAGGGTTTCACCCTGGCCCAGAAGATGGTGGGTAAAGCCTGTGGCGTGGCCGGTGTTCGCCCC
GGTCAATACTGTGAGCCCAAGATGACTTCTGTTGGTTCTCAGGACACCACAGGTCCTATGACCCGTGACGAGCTGAAAGA
CCTCGCATGTCTTGGCTTCAGCGCCGACCTGACCATGCAGTCATTCTGTCACACCGCCGCTTATCCAAAGCCAGTGGACG
TGAACACTCACCACACCCTGCCTGACTTCATCATGAACCGCGGTGGTATTTCACTGCGTCCAGGTGACGGTGTTATCCAC
TCATGGCTGAACCGCATGCTGCTGCCCGATACCGTAGGTACCGGCGGTGACTCGCACACCCGTTTCCCAATCGGTATCTC
CTTCCCGGCCGGTTCCGGTCTGGTAGCCTTCGCTGCTGCCACCGGCGTTATGCCACTGGATATGCCTGAGTCGGTACTGG
TGCGCTTCAAGGGTCAGATGCAGCCAGGTATCACCCTGCGTGATCTGGTGCATGCCATCCCGCACAAGGCCATCGAAATG
GGCCTGCTGACCGTTGAGAAGAAAGGCAAGAAGAACATCTTCTCTGGCCGCATCCTTGAAATCGAAGGTCTGGAACACCT
GAAAGTAGAGCAGGCGTTTGAGCTGTCTGATGCCTCTGCCGAGCGCTCTGCCGCCGGTTGTACCATCAAGCTGGATAAAG
ATCCTATCATCGAGTATCTGAACTCCAACATCGTCATGCTCAAGTGGATGATCGCTGAAGGCTACGGTGACCGCCGTACC
ATCGAGCGCCGCATCAAGGGCATGGAAGAATGGCTGAAGAACCCAGAGCTGATGGAAGCCGATAAGGATGCCGAGTACGC
TGCGGTTATCGAAATCGACCTGAACAACATCAAAGAGCCAATCCTGTGTGCCCCTAACGATCCGGACGATGCCGTGCTGC
TGTCTGCCGTTGCCAACACCAAGATTGACGAAGTGTTCGTCGGTTCCTGTATGACCAACATCGGCCACTTCCGTGCCACC
GGTAAGGTACTGGACAAGTTCGCCAAGAGCCTGCCAACCCGTCTGTGGATTGCCCCGCCAACCAAGATGGACAAGGACCA
GCTGACCGAAGAAGGCTACTACGGTATCTTCGGCCGTGTCGGTGCCCGTATCGAAATCCCGGGTTGTTCTCTGTGTATGG
GTAACCAGGCTCGCGTAGCCGATGGCGCCACTGTGGTGTCTACCTCTACCCGTAACTTCCCGAACCGTCTGGGTACCGGT
GCCAACGTCTACCTGGCCTCAGCGGAACTGGCCGCCGTAGCCGCCCTGCTGGGCCGTCTGCCATCGCCAGAGGAGTATCA
GGTATACGCCAAGGAAATCGACGCCACTGCCGCCGATACCTACCGCTACCTGAACTTCGATGAGATTGAGTCCTACACCA
AGAAAGCCGGTGAAGTGATCTTCCAATCTGCGGTCTAA

Upstream 100 bases:

>100_bases
TACTGCAGCCCGGAAATCCGGGTCGTCCTGTCGGACTGTAGCCTTCATCCACGAAGACGTGCAGTTTCGTTGAGAGCGCC
AAACAAAGAGGAATGTTGCC

Downstream 100 bases:

>100_bases
GTACTGACGCTTTGAGTCAAATGAAAAGCCGGCGCAAGCCGGCTTTTTTGTGCCTTTTCCCGTGTGTGGTATGTTGGCTA
AAGTGCAGGGCTGATAGTCG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MLEAYRKHVEERAAEGVVPKPLDAHQVAELVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAIAKGTATSPI
LNAERAVELLGTMQGGYNIEPLIHQLDNDKLAPLAVKALSHTLLMFDSFHDVVEKMNAGNAHAKQVVESWANAEWFLSRP
KLADKISLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEPGVVGPIKKIEELKTQGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYEGKVRRHG
SDEVISEFELKTDVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGASKEFVRPQDVADTGKGFTLAQKMVGKACGVAGVRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGISLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQMQPGITLRDLVHAIPHKAIEM
GLLTVEKKGKKNIFSGRILEIEGLEHLKVEQAFELSDASAERSAAGCTIKLDKDPIIEYLNSNIVMLKWMIAEGYGDRRT
IERRIKGMEEWLKNPELMEADKDAEYAAVIEIDLNNIKEPILCAPNDPDDAVLLSAVANTKIDEVFVGSCMTNIGHFRAT
GKVLDKFAKSLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPSPEEYQVYAKEIDATAADTYRYLNFDEIESYTKKAGEVIFQSAV

Sequences:

>Translated_865_residues
MLEAYRKHVEERAAEGVVPKPLDAHQVAELVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAIAKGTATSPI
LNAERAVELLGTMQGGYNIEPLIHQLDNDKLAPLAVKALSHTLLMFDSFHDVVEKMNAGNAHAKQVVESWANAEWFLSRP
KLADKISLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEPGVVGPIKKIEELKTQGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYEGKVRRHG
SDEVISEFELKTDVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGASKEFVRPQDVADTGKGFTLAQKMVGKACGVAGVRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGISLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQMQPGITLRDLVHAIPHKAIEM
GLLTVEKKGKKNIFSGRILEIEGLEHLKVEQAFELSDASAERSAAGCTIKLDKDPIIEYLNSNIVMLKWMIAEGYGDRRT
IERRIKGMEEWLKNPELMEADKDAEYAAVIEIDLNNIKEPILCAPNDPDDAVLLSAVANTKIDEVFVGSCMTNIGHFRAT
GKVLDKFAKSLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPSPEEYQVYAKEIDATAADTYRYLNFDEIESYTKKAGEVIFQSAV
>Mature_865_residues
MLEAYRKHVEERAAEGVVPKPLDAHQVAELVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAIAKGTATSPI
LNAERAVELLGTMQGGYNIEPLIHQLDNDKLAPLAVKALSHTLLMFDSFHDVVEKMNAGNAHAKQVVESWANAEWFLSRP
KLADKISLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEPGVVGPIKKIEELKTQGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYEGKVRRHG
SDEVISEFELKTDVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGASKEFVRPQDVADTGKGFTLAQKMVGKACGVAGVRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGISLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQMQPGITLRDLVHAIPHKAIEM
GLLTVEKKGKKNIFSGRILEIEGLEHLKVEQAFELSDASAERSAAGCTIKLDKDPIIEYLNSNIVMLKWMIAEGYGDRRT
IERRIKGMEEWLKNPELMEADKDAEYAAVIEIDLNNIKEPILCAPNDPDDAVLLSAVANTKIDEVFVGSCMTNIGHFRAT
GKVLDKFAKSLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPSPEEYQVYAKEIDATAADTYRYLNFDEIESYTKKAGEVIFQSAV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=82.3121387283237, Blast_Score=1453, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=387, Percent_Identity=23.7726098191214, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=27.2965879265092, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93899; Mature: 93899

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKHVEERAAEGVVPKPLDAHQVAELVELVKNPPAGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLDAIAKGTATSPILNAERAVELLGTMQGGYNIEPLIHQLDNDKLAPLAVKALS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHH
HTLLMFDSFHDVVEKMNAGNAHAKQVVESWANAEWFLSRPKLADKISLTVFKVTGETNTD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEEECCCCCC
DLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEPGVVGPIKKIEELKTQGFPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCEE
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEHHH
MEMGDVIDIYPYEGKVRRHGSDEVISEFELKTDVLLDEVRAGGRIPLIIGRGLTDKAREV
HCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LGLGASKEFVRPQDVADTGKGFTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPM
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGISLRPGDGVIH
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCHHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCCCCC
QPGITLRDLVHAIPHKAIEMGLLTVEKKGKKNIFSGRILEIEGLEHLKVEQAFELSDASA
CCCCHHHHHHHHCCHHHHHHHHEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCC
ERSAAGCTIKLDKDPIIEYLNSNIVMLKWMIAEGYGDRRTIERRIKGMEEWLKNPELMEA
HHCCCCCEEEECCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DKDAEYAAVIEIDLNNIKEPILCAPNDPDDAVLLSAVANTKIDEVFVGSCMTNIGHFRAT
CCCCCEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GKVLDKFAKSLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGNQARVA
HHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCCCHHHCCCCEEECCCCCEEECCCCEEC
DGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPEEYQVYAKEIDATAADT
CCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHH
YRYLNFDEIESYTKKAGEVIFQSAV
EEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEAYRKHVEERAAEGVVPKPLDAHQVAELVELVKNPPAGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLDAIAKGTATSPILNAERAVELLGTMQGGYNIEPLIHQLDNDKLAPLAVKALS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHH
HTLLMFDSFHDVVEKMNAGNAHAKQVVESWANAEWFLSRPKLADKISLTVFKVTGETNTD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEEECCCCCC
DLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEPGVVGPIKKIEELKTQGFPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCEE
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEHHH
MEMGDVIDIYPYEGKVRRHGSDEVISEFELKTDVLLDEVRAGGRIPLIIGRGLTDKAREV
HCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LGLGASKEFVRPQDVADTGKGFTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPM
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGISLRPGDGVIH
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCHHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCCCCC
QPGITLRDLVHAIPHKAIEMGLLTVEKKGKKNIFSGRILEIEGLEHLKVEQAFELSDASA
CCCCHHHHHHHHCCHHHHHHHHEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCC
ERSAAGCTIKLDKDPIIEYLNSNIVMLKWMIAEGYGDRRTIERRIKGMEEWLKNPELMEA
HHCCCCCEEEECCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DKDAEYAAVIEIDLNNIKEPILCAPNDPDDAVLLSAVANTKIDEVFVGSCMTNIGHFRAT
CCCCCEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GKVLDKFAKSLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGNQARVA
HHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCCCHHHCCCCEEECCCCCEEECCCCEEC
DGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPEEYQVYAKEIDATAADT
CCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHH
YRYLNFDEIESYTKKAGEVIFQSAV
EEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]