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The map label for this gene is tapD [H]

Identifier: 119773508

GI number: 119773508

Start: 443934

End: 444848

Strand: Reverse

Name: tapD [H]

Synonym: Sama_0367

Alternate gene names: 119773508

Gene position: 444848-443934 (Counterclockwise)

Preceding gene: 119773509

Following gene: 119773507

Centisome position: 10.33

GC content: 48.42

Gene sequence:

>915_bases
ATGACTGAATTTATCTCCCTAATGAGCCAGTTTCCCTGGCTCTTTATTTTGCTCGCATTCCTGTTCGCAGCCACCATTGG
TAGCTTCCTCAATGTCGTAATTCATCGCTTGCCTGTGATGATGAAAAGAGAATGGCAGCAGGAATGTAATGCCTATCTTG
AAGAGTACCAACCTGAACTTGTAAAAAAGCTTGGCAAAGCGAATTTGGACAAGCCAATTGATAGCTATCCAGAACGTTAT
AACCTGATTGTGCCAGGCTCATCCTGCCCTAAGTGCGGTACTATCATTAAGCCCTGGCACAATCTGCCTGTACTGGGCTG
GATAATCCTTGGCGGTAAATGCGCTTCCTGCAAAGCCCCAATTTCCAGCCGTTATCCCATAGTGGAAGCCTTTACCGGCC
TGACTGTTGCAGCACTGGCGTGGCACTTTGGTCCCACGTGGCAATTTGTCACAGCAGCATTACTCACCTTTGGCTTAATT
GCCATGTCGGGAATCGACCTGGATGAAATGCTACTGCCAGACAGCCTCACTCTTCCTCTGCTGTGGCTGGGATTACTGCT
TAATTATCATGGCCTATTTACCACTCTGGAAGATGCACTCATTGGTGCTGCAGCCGGTTATCTGTCGCTTTGGTCTGTGT
ATTGGCTGTTTAAGCTCACCACAGGCAAAGAGGGCATGGGCTACGGTGATTTTAAACTCATGGGGCTCTTTGGTGCTTGG
CTCGGCTGGCAAATGCTGCCGTTAATTATTCTTCTCTCAAGCCTGGTCGGTGCTGTCGTTGGCATACTGATGATGGTTTG
GAAAAGCCATAAGCGTGAAAATCCCATCCCATTTGGTCCCTATATCGCGGCCGCTGGCTGGATAGCACTTATTTGGGGAC
AAGATATTGTCAGCTGGTATCTGGGTTCTCTTTGA

Upstream 100 bases:

>100_bases
TTGTCGGCGGCCTGATTGTGGGTATGTATCTGCCTATCTTCCAGATGGGTAATGTGGTTGGCTAATAGCACTATTTTAAA
ACTTATAACAATTTTAACAG

Downstream 100 bases:

>100_bases
TGGCCGAGTTTATTCTGGGCCTCACCGGCGGAATCGGTAGCGGCAAGACAACTGTTGCTAACCTCTTTCACGAACAAGGC
ATTGAACTGGTTGATGCCGA

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTEFISLMSQFPWLFILLAFLFAATIGSFLNVVIHRLPVMMKREWQQECNAYLEEYQPELVKKLGKANLDKPIDSYPERY
NLIVPGSSCPKCGTIIKPWHNLPVLGWIILGGKCASCKAPISSRYPIVEAFTGLTVAALAWHFGPTWQFVTAALLTFGLI
AMSGIDLDEMLLPDSLTLPLLWLGLLLNYHGLFTTLEDALIGAAAGYLSLWSVYWLFKLTTGKEGMGYGDFKLMGLFGAW
LGWQMLPLIILLSSLVGAVVGILMMVWKSHKRENPIPFGPYIAAAGWIALIWGQDIVSWYLGSL

Sequences:

>Translated_304_residues
MTEFISLMSQFPWLFILLAFLFAATIGSFLNVVIHRLPVMMKREWQQECNAYLEEYQPELVKKLGKANLDKPIDSYPERY
NLIVPGSSCPKCGTIIKPWHNLPVLGWIILGGKCASCKAPISSRYPIVEAFTGLTVAALAWHFGPTWQFVTAALLTFGLI
AMSGIDLDEMLLPDSLTLPLLWLGLLLNYHGLFTTLEDALIGAAAGYLSLWSVYWLFKLTTGKEGMGYGDFKLMGLFGAW
LGWQMLPLIILLSSLVGAVVGILMMVWKSHKRENPIPFGPYIAAAGWIALIWGQDIVSWYLGSL
>Mature_303_residues
TEFISLMSQFPWLFILLAFLFAATIGSFLNVVIHRLPVMMKREWQQECNAYLEEYQPELVKKLGKANLDKPIDSYPERYN
LIVPGSSCPKCGTIIKPWHNLPVLGWIILGGKCASCKAPISSRYPIVEAFTGLTVAALAWHFGPTWQFVTAALLTFGLIA
MSGIDLDEMLLPDSLTLPLLWLGLLLNYHGLFTTLEDALIGAAAGYLSLWSVYWLFKLTTGKEGMGYGDFKLMGLFGAWL
GWQMLPLIILLSSLVGAVVGILMMVWKSHKRENPIPFGPYIAAAGWIALIWGQDIVSWYLGSL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=282, Percent_Identity=30.8510638297872, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1789732, Length=143, Percent_Identity=46.8531468531469, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 33881; Mature: 33749

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFISLMSQFPWLFILLAFLFAATIGSFLNVVIHRLPVMMKREWQQECNAYLEEYQPEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKKLGKANLDKPIDSYPERYNLIVPGSSCPKCGTIIKPWHNLPVLGWIILGGKCASCKAP
HHHHHHCCCCCCHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCC
ISSRYPIVEAFTGLTVAALAWHFGPTWQFVTAALLTFGLIAMSGIDLDEMLLPDSLTLPL
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHH
LWLGLLLNYHGLFTTLEDALIGAAAGYLSLWSVYWLFKLTTGKEGMGYGDFKLMGLFGAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LGWQMLPLIILLSSLVGAVVGILMMVWKSHKRENPIPFGPYIAAAGWIALIWGQDIVSWY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGSL
HCCC
>Mature Secondary Structure 
TEFISLMSQFPWLFILLAFLFAATIGSFLNVVIHRLPVMMKREWQQECNAYLEEYQPEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA