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The map label for this gene is murF [H]

Identifier: 119773491

GI number: 119773491

Start: 419389

End: 420756

Strand: Direct

Name: murF [H]

Synonym: Sama_0350

Alternate gene names: 119773491

Gene position: 419389-420756 (Clockwise)

Preceding gene: 119773490

Following gene: 119773492

Centisome position: 9.74

GC content: 56.36

Gene sequence:

>1368_bases
GTGATCCCTCTGAGTCTGACTCAATTGGCGGCAGCCACCCATGGGCGTCTTTGTGGCGAGGATGTTGTCGTGTCACATCT
CGACAGCGACAGCCGAAAAATGACCGTGGGCAGCTTGTTTGTGGCATTGAAGGGTGAGCGTTTTGACGGCCATGAGTTTG
CCGAAGCCGCCGCAGCAGCAGGGGCATCAGCGCTTTTGGTTGAACGTGAGCTGCCGCTCGCGCTGCCACAACTGGTGGTT
GCCGATACACAAAAGGCCATGGGCCAAATTGGCGCCCTGGTGCGCGACAGCGTAAATCCTGTTTGTGTGGCACTGACCGG
CTCAAACGGCAAAACCAGTGTAAAAGAGATGGTCGCCACCGTGCTGTCGCAACATCATTCCGTGCTCTTTACCGCAGGTA
ACTTTAACAATGAAATCGGTGTGCCTCTAACCCTGCTGCGTCTGGAGCAGGGGCACGAGTATGGGGTATTTGAGCTGGGT
GCGAACCACAAGGGCGAGATTGATTACACCTCATCACTGGTTCGTCCTTCGGTGTCACTGGTCAACAATGTGGGCAGCGC
TCACCTTGAAGGTTTTGGCTCAGAGGCCGGTGTTGCCGAGGCCAAGTCCGAAATCTATCTGCATCTGGCGCAGGACGGTG
TGGGGATTGTGAATCTCGACGACCGCTACGCAGAGACCATGCTCAACAAGCTCAAGGGAAAGCGGGTACTGACCTTTGGG
CTGGGTGCCGGGGCCGAGATTACCGCCCGGGAAGTGACTGCCGATGCCTTCGGCAGATACAGCTTTTACCTCTGCCATGG
CGGAGACCATGTGACTGTGAGTTTGCCGCTGGCAGGCAAGCATCAGGTGTCCAATGCGCTGGCAACGGCCGCCATCTGCA
TTGCCCTGGGCTTACCTTTGCAAGAGATAGCAGCGGGTCTTGCCAAACTGGCTCCGGTCAAGGGACGTATGATGCCACGC
CAGTTGGGACGACTGCTGTTGGTGGACGACAGCTACAATGCCAATCCCAATTCAGTGGGGGCTGCCATCGACTGGCTGAA
AGAAATTTCTACAAACAGGACCCTGGTACTGGGCGATTTGGGTGAATTAGGCGACAATGCTGCCCTTTTGCACTTTGAGC
TGGGGCAGAAGGCCAAAGAAGCCGGGCTCGACAGCCTGTTTTGTCTGGGCAGTTTGAGCCGCCATGCCAGCCAAGCCTTT
GGCAGTAAACATTTCGAAGAGCTGGATGCTCTCGTGAGTGAATTGATTAACTATATCAACGGGGTAGAGGGAGATGTGAC
CCTGTTGGTGAAAGGGTCTCGCAGCGCCGCCATGGAGCGGGTTGTAGAGGCCTTAACAGCCGCCCATGGGCGCGGGGAGC
TGAAATAA

Upstream 100 bases:

>100_bases
TGCTGGCCGGTAAGGGGCATGAGACCTATCAAGAGATAGCCGGTGTTAAACATGAATATGATGAGCGTGCCCTGGCACGG
GTGTTAAGCGAGGAAAATCT

Downstream 100 bases:

>100_bases
ATGCTGGTTTATCTGGCCGAATATCTGACCCAGTTTTACACCGGGTTTAACGTGTTTTCCTATGTGACCTTCAGAGCCAT
TCTGGCGCTGCTGACGGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl- D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MIPLSLTQLAAATHGRLCGEDVVVSHLDSDSRKMTVGSLFVALKGERFDGHEFAEAAAAAGASALLVERELPLALPQLVV
ADTQKAMGQIGALVRDSVNPVCVALTGSNGKTSVKEMVATVLSQHHSVLFTAGNFNNEIGVPLTLLRLEQGHEYGVFELG
ANHKGEIDYTSSLVRPSVSLVNNVGSAHLEGFGSEAGVAEAKSEIYLHLAQDGVGIVNLDDRYAETMLNKLKGKRVLTFG
LGAGAEITAREVTADAFGRYSFYLCHGGDHVTVSLPLAGKHQVSNALATAAICIALGLPLQEIAAGLAKLAPVKGRMMPR
QLGRLLLVDDSYNANPNSVGAAIDWLKEISTNRTLVLGDLGELGDNAALLHFELGQKAKEAGLDSLFCLGSLSRHASQAF
GSKHFEELDALVSELINYINGVEGDVTLLVKGSRSAAMERVVEALTAAHGRGELK

Sequences:

>Translated_455_residues
MIPLSLTQLAAATHGRLCGEDVVVSHLDSDSRKMTVGSLFVALKGERFDGHEFAEAAAAAGASALLVERELPLALPQLVV
ADTQKAMGQIGALVRDSVNPVCVALTGSNGKTSVKEMVATVLSQHHSVLFTAGNFNNEIGVPLTLLRLEQGHEYGVFELG
ANHKGEIDYTSSLVRPSVSLVNNVGSAHLEGFGSEAGVAEAKSEIYLHLAQDGVGIVNLDDRYAETMLNKLKGKRVLTFG
LGAGAEITAREVTADAFGRYSFYLCHGGDHVTVSLPLAGKHQVSNALATAAICIALGLPLQEIAAGLAKLAPVKGRMMPR
QLGRLLLVDDSYNANPNSVGAAIDWLKEISTNRTLVLGDLGELGDNAALLHFELGQKAKEAGLDSLFCLGSLSRHASQAF
GSKHFEELDALVSELINYINGVEGDVTLLVKGSRSAAMERVVEALTAAHGRGELK
>Mature_455_residues
MIPLSLTQLAAATHGRLCGEDVVVSHLDSDSRKMTVGSLFVALKGERFDGHEFAEAAAAAGASALLVERELPLALPQLVV
ADTQKAMGQIGALVRDSVNPVCVALTGSNGKTSVKEMVATVLSQHHSVLFTAGNFNNEIGVPLTLLRLEQGHEYGVFELG
ANHKGEIDYTSSLVRPSVSLVNNVGSAHLEGFGSEAGVAEAKSEIYLHLAQDGVGIVNLDDRYAETMLNKLKGKRVLTFG
LGAGAEITAREVTADAFGRYSFYLCHGGDHVTVSLPLAGKHQVSNALATAAICIALGLPLQEIAAGLAKLAPVKGRMMPR
QLGRLLLVDDSYNANPNSVGAAIDWLKEISTNRTLVLGDLGELGDNAALLHFELGQKAKEAGLDSLFCLGSLSRHASQAF
GSKHFEELDALVSELINYINGVEGDVTLLVKGSRSAAMERVVEALTAAHGRGELK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=448, Percent_Identity=48.2142857142857, Blast_Score=354, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47959; Mature: 47959

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLSLTQLAAATHGRLCGEDVVVSHLDSDSRKMTVGSLFVALKGERFDGHEFAEAAAAA
CCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHC
GASALLVERELPLALPQLVVADTQKAMGQIGALVRDSVNPVCVALTGSNGKTSVKEMVAT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
VLSQHHSVLFTAGNFNNEIGVPLTLLRLEQGHEYGVFELGANHKGEIDYTSSLVRPSVSL
HHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHCCHHHH
VNNVGSAHLEGFGSEAGVAEAKSEIYLHLAQDGVGIVNLDDRYAETMLNKLKGKRVLTFG
HHCCCCHHHHCCCCCCCHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHHHCCCEEEEEE
LGAGAEITAREVTADAFGRYSFYLCHGGDHVTVSLPLAGKHQVSNALATAAICIALGLPL
CCCCCEEEHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCH
QEIAAGLAKLAPVKGRMMPRQLGRLLLVDDSYNANPNSVGAAIDWLKEISTNRTLVLGDL
HHHHHHHHHHCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
GELGDNAALLHFELGQKAKEAGLDSLFCLGSLSRHASQAFGSKHFEELDALVSELINYIN
CCCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVEGDVTLLVKGSRSAAMERVVEALTAAHGRGELK
CCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIPLSLTQLAAATHGRLCGEDVVVSHLDSDSRKMTVGSLFVALKGERFDGHEFAEAAAAA
CCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHC
GASALLVERELPLALPQLVVADTQKAMGQIGALVRDSVNPVCVALTGSNGKTSVKEMVAT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
VLSQHHSVLFTAGNFNNEIGVPLTLLRLEQGHEYGVFELGANHKGEIDYTSSLVRPSVSL
HHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHCCHHHH
VNNVGSAHLEGFGSEAGVAEAKSEIYLHLAQDGVGIVNLDDRYAETMLNKLKGKRVLTFG
HHCCCCHHHHCCCCCCCHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHHHCCCEEEEEE
LGAGAEITAREVTADAFGRYSFYLCHGGDHVTVSLPLAGKHQVSNALATAAICIALGLPL
CCCCCEEEHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCH
QEIAAGLAKLAPVKGRMMPRQLGRLLLVDDSYNANPNSVGAAIDWLKEISTNRTLVLGDL
HHHHHHHHHHCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
GELGDNAALLHFELGQKAKEAGLDSLFCLGSLSRHASQAFGSKHFEELDALVSELINYIN
CCCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVEGDVTLLVKGSRSAAMERVVEALTAAHGRGELK
CCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]