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The map label for this gene is glpK

Identifier: 119773482

GI number: 119773482

Start: 410502

End: 411989

Strand: Direct

Name: glpK

Synonym: Sama_0341

Alternate gene names: 119773482

Gene position: 410502-411989 (Clockwise)

Preceding gene: 119773481

Following gene: 119773486

Centisome position: 9.53

GC content: 56.79

Gene sequence:

>1488_bases
GTGGACAAGCAATATGTGGTTGCGCTGGATCAGGGCACAACCAGTTCCCGCGCCATTGTTTTTGACCGGGATGCCAACCT
GGTGGCGGTGTCGCAGCGCGAATTCACTCAAATTTACCCCAAGGGCGGCTGGGTCGAGCACGACCCCATGGAGATCTGGG
CATCCCAGAGCTCGACCCTGATTGAAGTCCTGGCCCGTGCCGGTATTCACAGCGATGAAGTGGCTGCTATAGGTATCACC
AATCAGCGTGAAACCACCATCATCTGGGACAAACTCACGGGTAAACCTGTGTGCAATGCCATCGTATGGCAGTGCCGCCG
CAGTGCCGCGATCTGTGAAGCGCTTAAAGCTCAGGGTTTGGAGCCGCTGTTTCGCGATAAAACAGGCCTCCTGCTCGACC
CTTATTTTTCCGGCACCAAAATCAAATGGATTCTGGACAATGTGCCCGGTGTGCGTGAGCGTGCCGACGCGGGTGAGCTG
CTGTTTGGCACCGTGGATACCTGGCTGGTGTGGAAACTCACCGAAGGTAAGGTCCATGTCACCGACCCCACCAATGCTTC
ACGCACCCTGATGTACAATATCCATTCTGGCGAATGGGACTCGGAGTTGCTGAAGGCGCTGGATATTCCAGCCAGCTTAT
TGCCAGAGGTAAAGTCGTCTCAGGCGGTGTATGGCACCACCCGTATCGCCGGTGAGGGTACTGAAATCCCGGTGGCAGGT
ATGGCCGGCGATCAGCAGGCGGCGTTGTTTGGCCAGCTGTGTGTCGAGCCCGGTATGGCCAAAAATACCTACGGTACCGG
CTGCTTTTTGCTGATGAATACGGGTAAACAAGCTGTCAAATCAGAGCATGGTCTGCTGACTACCATTGCCGTGGGCGATG
ATGGTGGTATCAGCTATGCACTGGAGGGCGCCGTGTTTATGGGCGGTGCCACCATTCAGTGGCTTAGGGACGAGCTGGGG
CTGATTCGTGATGCCAGCGATACCGAATATTTTGCGTCCAAGGTTGACGACACTAATGGCGTGTATCTGGTACCGGCCTT
TGTGGGCCTTGGCGCGCCTTATTGGGATCCGGATGCCCGCGGCACCTTGGTTGGGCTGACCCGCGGTGCTAACCGTAACC
ACATCATCCGGGCGGCGCTGGAATCCATTGCCTACCAAAGCCGTGACCTGCTGGATGCCATGAGCAAAGACAGCGGCGTG
GCGCTCAAACAGCTCAAGGTGGATGGCGGCGCGGTAGCCAACGATTTTTTGATGCAGTTTCAGGCCGATATCTCGGGAGT
TGAAGTGCTGCGCCCGGCACAGACGGAAACCACCGCTATGGGCGCGGCGTTCCTTGGTGGTCTCGCCGTCGGTTTCTGGT
CCAATGTGGATGAAATACGGCACAAGAGCGGCATAGACAGGCGCTTTGTGCCACTGATGGATGATAAAGAAAGAGCGACG
CTTTACCTTGGATGGCAAGATGCTGTGCGCCGAAGCCTGAGTAGCTGA

Upstream 100 bases:

>100_bases
TTCGTCTATCTGGTATTACCCTGCCCATGGCTGTGAACCCAGAGACACCGTGATGGAAGAGGCGCCAGAAGTACCGACAC
AAGAATAAAAGGACTGCCCC

Downstream 100 bases:

>100_bases
TTTTAAAGCGTTATTTGTCAGCAACTAAACTTCAATATCGATATGACTACCAGTGCGGCGGGCGCGCCGCTCTACTGCTG
GCTTACTGACACTCTCCCTG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MDKQYVVALDQGTTSSRAIVFDRDANLVAVSQREFTQIYPKGGWVEHDPMEIWASQSSTLIEVLARAGIHSDEVAAIGIT
NQRETTIIWDKLTGKPVCNAIVWQCRRSAAICEALKAQGLEPLFRDKTGLLLDPYFSGTKIKWILDNVPGVRERADAGEL
LFGTVDTWLVWKLTEGKVHVTDPTNASRTLMYNIHSGEWDSELLKALDIPASLLPEVKSSQAVYGTTRIAGEGTEIPVAG
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGKQAVKSEHGLLTTIAVGDDGGISYALEGAVFMGGATIQWLRDELG
LIRDASDTEYFASKVDDTNGVYLVPAFVGLGAPYWDPDARGTLVGLTRGANRNHIIRAALESIAYQSRDLLDAMSKDSGV
ALKQLKVDGGAVANDFLMQFQADISGVEVLRPAQTETTAMGAAFLGGLAVGFWSNVDEIRHKSGIDRRFVPLMDDKERAT
LYLGWQDAVRRSLSS

Sequences:

>Translated_495_residues
MDKQYVVALDQGTTSSRAIVFDRDANLVAVSQREFTQIYPKGGWVEHDPMEIWASQSSTLIEVLARAGIHSDEVAAIGIT
NQRETTIIWDKLTGKPVCNAIVWQCRRSAAICEALKAQGLEPLFRDKTGLLLDPYFSGTKIKWILDNVPGVRERADAGEL
LFGTVDTWLVWKLTEGKVHVTDPTNASRTLMYNIHSGEWDSELLKALDIPASLLPEVKSSQAVYGTTRIAGEGTEIPVAG
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGKQAVKSEHGLLTTIAVGDDGGISYALEGAVFMGGATIQWLRDELG
LIRDASDTEYFASKVDDTNGVYLVPAFVGLGAPYWDPDARGTLVGLTRGANRNHIIRAALESIAYQSRDLLDAMSKDSGV
ALKQLKVDGGAVANDFLMQFQADISGVEVLRPAQTETTAMGAAFLGGLAVGFWSNVDEIRHKSGIDRRFVPLMDDKERAT
LYLGWQDAVRRSLSS
>Mature_495_residues
MDKQYVVALDQGTTSSRAIVFDRDANLVAVSQREFTQIYPKGGWVEHDPMEIWASQSSTLIEVLARAGIHSDEVAAIGIT
NQRETTIIWDKLTGKPVCNAIVWQCRRSAAICEALKAQGLEPLFRDKTGLLLDPYFSGTKIKWILDNVPGVRERADAGEL
LFGTVDTWLVWKLTEGKVHVTDPTNASRTLMYNIHSGEWDSELLKALDIPASLLPEVKSSQAVYGTTRIAGEGTEIPVAG
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGKQAVKSEHGLLTTIAVGDDGGISYALEGAVFMGGATIQWLRDELG
LIRDASDTEYFASKVDDTNGVYLVPAFVGLGAPYWDPDARGTLVGLTRGANRNHIIRAALESIAYQSRDLLDAMSKDSGV
ALKQLKVDGGAVANDFLMQFQADISGVEVLRPAQTETTAMGAAFLGGLAVGFWSNVDEIRHKSGIDRRFVPLMDDKERAT
LYLGWQDAVRRSLSS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=45.8498023715415, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI189409120, Length=501, Percent_Identity=46.1077844311377, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI4504007, Length=501, Percent_Identity=46.1077844311377, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI41393575, Length=500, Percent_Identity=47, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=32.6771653543307, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1790361, Length=494, Percent_Identity=69.2307692307692, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=515, Percent_Identity=25.631067961165, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1790005, Length=473, Percent_Identity=23.8900634249471, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789987, Length=495, Percent_Identity=23.030303030303, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1789138, Length=497, Percent_Identity=22.7364185110664, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=498, Percent_Identity=45.1807228915663, Blast_Score=413, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=24.6153846153846, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=264, Percent_Identity=29.5454545454545, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=552, Percent_Identity=37.6811594202899, Blast_Score=309, Evalue=6e-85,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=43.7623762376238, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=41.9678714859438, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=41.9678714859438, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=41.9678714859438, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=41.9678714859438, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=41.9678714859438, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24654573, Length=442, Percent_Identity=45.0226244343891, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI19922058, Length=523, Percent_Identity=32.3135755258126, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24652871, Length=490, Percent_Identity=32.8571428571429, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24656367, Length=495, Percent_Identity=28.2828282828283, Blast_Score=202, Evalue=6e-52,
Organism=Drosophila melanogaster, GI21357867, Length=510, Percent_Identity=27.6470588235294, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24656372, Length=330, Percent_Identity=31.5151515151515, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=28.6206896551724, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_SHEAM (A1S2E6)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926222.1
- ProteinModelPortal:   A1S2E6
- SMR:   A1S2E6
- STRING:   A1S2E6
- GeneID:   4602597
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0341
- NMPDR:   fig|326297.7.peg.324
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   ATYFSGT
- PhylomeDB:   A1S2E6
- ProtClustDB:   CLSK907562
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 53755; Mature: 53755

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKQYVVALDQGTTSSRAIVFDRDANLVAVSQREFTQIYPKGGWVEHDPMEIWASQSSTL
CCCCEEEEEECCCCCCEEEEEECCCCEEEECCCHHHEECCCCCCCCCCHHHHCCCCCHHH
IEVLARAGIHSDEVAAIGITNQRETTIIWDKLTGKPVCNAIVWQCRRSAAICEALKAQGL
HHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EPLFRDKTGLLLDPYFSGTKIKWILDNVPGVRERADAGELLFGTVDTWLVWKLTEGKVHV
CHHHCCCCCCEECCCCCCCEEEEEECCCCCCHHHCCCCCEEEEECCEEEEEEEECCEEEE
TDPTNASRTLMYNIHSGEWDSELLKALDIPASLLPEVKSSQAVYGTTRIAGEGTEIPVAG
ECCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHCHHCCCCEEEEEEEECCCCCCCEEEC
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGKQAVKSEHGLLTTIAVGDDGGISYA
CCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHCCCCEEEEEEECCCCCEEEE
LEGAVFMGGATIQWLRDELGLIRDASDTEYFASKVDDTNGVYLVPAFVGLGAPYWDPDAR
ECCEEEECCCHHHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCC
GTLVGLTRGANRNHIIRAALESIAYQSRDLLDAMSKDSGVALKQLKVDGGAVANDFLMQF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHH
QADISGVEVLRPAQTETTAMGAAFLGGLAVGFWSNVDEIRHKSGIDRRFVPLMDDKERAT
HCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCEE
LYLGWQDAVRRSLSS
EEEEHHHHHHHHHCC
>Mature Secondary Structure
MDKQYVVALDQGTTSSRAIVFDRDANLVAVSQREFTQIYPKGGWVEHDPMEIWASQSSTL
CCCCEEEEEECCCCCCEEEEEECCCCEEEECCCHHHEECCCCCCCCCCHHHHCCCCCHHH
IEVLARAGIHSDEVAAIGITNQRETTIIWDKLTGKPVCNAIVWQCRRSAAICEALKAQGL
HHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EPLFRDKTGLLLDPYFSGTKIKWILDNVPGVRERADAGELLFGTVDTWLVWKLTEGKVHV
CHHHCCCCCCEECCCCCCCEEEEEECCCCCCHHHCCCCCEEEEECCEEEEEEEECCEEEE
TDPTNASRTLMYNIHSGEWDSELLKALDIPASLLPEVKSSQAVYGTTRIAGEGTEIPVAG
ECCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHCHHCCCCEEEEEEEECCCCCCCEEEC
MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGKQAVKSEHGLLTTIAVGDDGGISYA
CCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHCCCCEEEEEEECCCCCEEEE
LEGAVFMGGATIQWLRDELGLIRDASDTEYFASKVDDTNGVYLVPAFVGLGAPYWDPDAR
ECCEEEECCCHHHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCC
GTLVGLTRGANRNHIIRAALESIAYQSRDLLDAMSKDSGVALKQLKVDGGAVANDFLMQF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHH
QADISGVEVLRPAQTETTAMGAAFLGGLAVGFWSNVDEIRHKSGIDRRFVPLMDDKERAT
HCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCEE
LYLGWQDAVRRSLSS
EEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA