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The map label for this gene is leuC

Identifier: 119773478

GI number: 119773478

Start: 406008

End: 407420

Strand: Direct

Name: leuC

Synonym: Sama_0337

Alternate gene names: 119773478

Gene position: 406008-407420 (Clockwise)

Preceding gene: 119773477

Following gene: 119773479

Centisome position: 9.43

GC content: 60.58

Gene sequence:

>1413_bases
ATGGCAAAGACTCTGTATGAGAAAGTGTGGGACGCCCACCTGGTGGTTGAGCCAGCCGGTGAAGCGCCCATCATCTATGT
AGACCGGCATCTGGTGCACGAGGTGACCTCACCTCAGGCATTCAGTGGCCTGAAGATGGCCGGTCGCCCACTGCGGGCAG
TGGAAAAAACCTTTGCCACCATGGATCACAATACCTCTACCAAGAGCGCCAGCTTAACAGCATTAAGCCCCATGGCCCGC
ACTCAGGTAGAGACCCTGGCCGACAACTGCCGCGACTTTAATGTGCGTTTGTACGACATTCACCATCCCAATCAAGGCAT
TGTGCACGTGATGGGCCCCGAGCTTGGCATTACCCTGCCGGGCACTGTGATTGTGTGCGGCGACTCCCACACCGCCACCC
ACGGCGCATTCGGTGCGCTGGCCTTCGGTATCGGCACCTCGGAAGTGGAGCACGTACTGGCTACCCAGACCCTGCGTCAG
CTCAAGGCCAAGACCATGAAAATCGAGGTGCGCGGCAAGGTGGCCGATGGCATCACCGCCAAGGACATAGTGCTGGCCAT
TATTGGTAAAATCGGCATGGATGGCGGCACAGGTTATGTGGTGGAGTTCTGCGGCGAGGCCATTCGTGACCTCTCCATGG
AGGGGCGTATGACCCTGTGTAATATGGCTATCGAAATGGGTGCCAAGGCCGGTATGGTGGCCCCCGATGAAACCACTTTC
GCTTACCTCGAAGGTCGTGAGTTCGCGCCCAAGGGTGATGCCTGGCAGCAGGCGCTGGCCGACTGGCGCGAGCTGCACAC
GGATGCCGATGCGGTATTTGATGCCGAAGTGGTACTGGAAGCGGCCGCGATTGCGCCGCAGCTGACCTGGGGCACTAACC
CGGGGCAGGTGGTGGCCATCGATGGCGTGGTGCCAAACCCCGCCGATGAAACCAATCCCGTGGTGCGCACCAGCATGGAG
AAGGCACTGGCCTATGTGGATTTGACCCCGGGCACCCCCATGACCGATATCGCCATCAACAAGGTGTTTATCGGCTCCTG
CACCAACTCCCGTATCGAAGACTTGCGCGCTGCGGCGGCGCAGGCCAAGGGACGCAAGGTTGCCAGTGGCGTAACCGCCA
TTGTGGTGCCGGGCTCAGGTCAGGTAAAACTGCAGGCCGAGGCCGAGGGCCTGGACAAAATCTTCCTTGAGGCGGGTTTT
GAGTGGCGCCTGCCGGGCTGCTCCATGTGTTTGGCGATGAACGATGACAGGCTGGAAGCGGGCGATCGCTGCGCCTCCAC
CAGTAACCGTAACTTTGAAGGACGCCAGGGGCGGGGCAGCCGCACCCATCTGGTGAGCCCTGCCATGGCCGCTGCGGCCG
CGGTTGCCGGTCACTTTGTCGATATTCGCGTTCACCGTCCTCTGGAGGATTAA

Upstream 100 bases:

>100_bases
TCTTAACCGGCGAGCTGCTGTCTGCTGACCAGCGTCACAATGCTAAATCAACCAAAGAAATGGGCGACTTTATCGCCAAT
GCAATCCGGGAAGGTGTGTA

Downstream 100 bases:

>100_bases
TCATGCAGGCATTTACTACTCACACGGGGCTGGCGGTGGCCATAGATTCGGCCAACATAGACACGGATCAGATTATCCCC
AAGCAGTTTTTGTCCAAGGT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAKTLYEKVWDAHLVVEPAGEAPIIYVDRHLVHEVTSPQAFSGLKMAGRPLRAVEKTFATMDHNTSTKSASLTALSPMAR
TQVETLADNCRDFNVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQ
LKAKTMKIEVRGKVADGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGMVAPDETTF
AYLEGREFAPKGDAWQQALADWRELHTDADAVFDAEVVLEAAAIAPQLTWGTNPGQVVAIDGVVPNPADETNPVVRTSME
KALAYVDLTPGTPMTDIAINKVFIGSCTNSRIEDLRAAAAQAKGRKVASGVTAIVVPGSGQVKLQAEAEGLDKIFLEAGF
EWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRVHRPLED

Sequences:

>Translated_470_residues
MAKTLYEKVWDAHLVVEPAGEAPIIYVDRHLVHEVTSPQAFSGLKMAGRPLRAVEKTFATMDHNTSTKSASLTALSPMAR
TQVETLADNCRDFNVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQ
LKAKTMKIEVRGKVADGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGMVAPDETTF
AYLEGREFAPKGDAWQQALADWRELHTDADAVFDAEVVLEAAAIAPQLTWGTNPGQVVAIDGVVPNPADETNPVVRTSME
KALAYVDLTPGTPMTDIAINKVFIGSCTNSRIEDLRAAAAQAKGRKVASGVTAIVVPGSGQVKLQAEAEGLDKIFLEAGF
EWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRVHRPLED
>Mature_469_residues
AKTLYEKVWDAHLVVEPAGEAPIIYVDRHLVHEVTSPQAFSGLKMAGRPLRAVEKTFATMDHNTSTKSASLTALSPMART
QVETLADNCRDFNVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQL
KAKTMKIEVRGKVADGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGMVAPDETTFA
YLEGREFAPKGDAWQQALADWRELHTDADAVFDAEVVLEAAAIAPQLTWGTNPGQVVAIDGVVPNPADETNPVVRTSMEK
ALAYVDLTPGTPMTDIAINKVFIGSCTNSRIEDLRAAAAQAKGRKVASGVTAIVVPGSGQVKLQAEAEGLDKIFLEAGFE
WRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRVHRPLED

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=368, Percent_Identity=28.2608695652174, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI8659555, Length=405, Percent_Identity=26.1728395061728, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI41352693, Length=364, Percent_Identity=26.6483516483516, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=73.866090712743, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=370, Percent_Identity=29.1891891891892, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI2367097, Length=358, Percent_Identity=25.1396648044693, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=477, Percent_Identity=26.2054507337526, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=368, Percent_Identity=27.445652173913, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=27.0358306188925, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=449, Percent_Identity=25.6124721603563, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=468, Percent_Identity=60.8974358974359, Blast_Score=568, Evalue=1e-163,
Organism=Saccharomyces cerevisiae, GI6320440, Length=461, Percent_Identity=25.8134490238612, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=402, Percent_Identity=29.8507462686567, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=366, Percent_Identity=29.5081967213115, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI281365315, Length=474, Percent_Identity=25.3164556962025, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI17864292, Length=474, Percent_Identity=25.3164556962025, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161076999, Length=390, Percent_Identity=25.6410256410256, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28571643, Length=477, Percent_Identity=26.2054507337526, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24645686, Length=410, Percent_Identity=26.8292682926829, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137564, Length=416, Percent_Identity=25.9615384615385, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SHEAM (A1S2E2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926218.1
- STRING:   A1S2E2
- GeneID:   4602593
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0337
- NMPDR:   fig|326297.7.peg.320
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   A1S2E2
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50183; Mature: 50052

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKVWDAHLVVEPAGEAPIIYVDRHLVHEVTSPQAFSGLKMAGRPLRAVEKTFAT
CCHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHCCHHHCCCCHHHHHHHHHH
MDHNTSTKSASLTALSPMARTQVETLADNCRDFNVRLYDIHHPNQGIVHVMGPELGITLP
CCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCEECC
GTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQLKAKTMKIEVRGKVADGITA
CEEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KDIVLAIIGKIGMDGGTGYVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGMVAPDETTF
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AYLEGREFAPKGDAWQQALADWRELHTDADAVFDAEVVLEAAAIAPQLTWGTNPGQVVAI
EEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEE
DGVVPNPADETNPVVRTSMEKALAYVDLTPGTPMTDIAINKVFIGSCTNSRIEDLRAAAA
CCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCHHEEEEEEEEECCCCHHHHHHHHHHH
QAKGRKVASGVTAIVVPGSGQVKLQAEAEGLDKIFLEAGFEWRLPGCSMCLAMNDDRLEA
HHCCCEECCCCEEEEECCCCCEEEEECCCCHHHHHHHCCCEEECCCCEEEEEECCCCCCC
GDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRVHRPLED
CCHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHCEEEEEEECCCCCC
>Mature Secondary Structure 
AKTLYEKVWDAHLVVEPAGEAPIIYVDRHLVHEVTSPQAFSGLKMAGRPLRAVEKTFAT
CHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHCCHHHCCCCHHHHHHHHHH
MDHNTSTKSASLTALSPMARTQVETLADNCRDFNVRLYDIHHPNQGIVHVMGPELGITLP
CCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCEECC
GTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQLKAKTMKIEVRGKVADGITA
CEEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KDIVLAIIGKIGMDGGTGYVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGMVAPDETTF
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AYLEGREFAPKGDAWQQALADWRELHTDADAVFDAEVVLEAAAIAPQLTWGTNPGQVVAI
EEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEE
DGVVPNPADETNPVVRTSMEKALAYVDLTPGTPMTDIAINKVFIGSCTNSRIEDLRAAAA
CCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCHHEEEEEEEEECCCCHHHHHHHHHHH
QAKGRKVASGVTAIVVPGSGQVKLQAEAEGLDKIFLEAGFEWRLPGCSMCLAMNDDRLEA
HHCCCEECCCCEEEEECCCCCEEEEECCCCHHHHHHHCCCEEECCCCEEEEEECCCCCCC
GDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRVHRPLED
CCHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA