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The map label for this gene is ybiK [C]

Identifier: 119773440

GI number: 119773440

Start: 361818

End: 362783

Strand: Direct

Name: ybiK [C]

Synonym: Sama_0299

Alternate gene names: 119773440

Gene position: 361818-362783 (Clockwise)

Preceding gene: 119773439

Following gene: 119773441

Centisome position: 8.4

GC content: 59.73

Gene sequence:

>966_bases
ATGGCCTCAAGAAGGGACTTTCTCAAACTCTCGTCGCTGGCATCCATGATAGGCCTGGCAGCGCCCGCCATGGCGCTGGG
TCGTAAGGTGAATAAACCCATAGTGGTCTCCACCTGGGAGCACGGTATGCCCGCCAACGAAGCGGCCTGGCAAGTGCTCA
ACGCAGGCGGCAGAGCCCTGGATGCTGTAGAGGCCGGTGTGCGGGTACCCGAGGCCGATCCCAAAGTACGTACTGTGGGA
TACGGTGGTTATCCCGACAGAGACGGCAAGGTGACTCTGGATGCCTGCATCATGGATGAACACATGAACTGTGGTTCGGT
GGCGTTTTTGCAGGGTATCAAGCACCCCATATCGGTGGCACGTCTGGTGATGGAAAAGACCCCCCATGTGATGCTGGTGG
GGGAAGGCGCCAGGCAGTTTGCCCTGTCGCAGGGCTTTGTTGAAGAAGAGCTGCTGACAGCAGAGGCCAGGGCCGACTGG
CAGCGCTGGCTGAAAGAGCAGACAGTGCAGACCATCAACATTGAGAACCACGACACCATAGGCATGCTGGCGCTGGACGC
GAACGGTAATCTCAGCGGCGCCTGCACCACCAGCGGCGCAGCTTACAAGATGCATGGCCGCGTGGGCGACTCTCCCCTGA
TTGGTGCCGGGCTGTATGTGGATAACGACGTTGGCGCCGCCACCGCCACCGGCATGGGCGAGCTGATGATTAAGACAGTG
GGCTGCCACTTGGTGGTGGAGCTGATGCGTCAGGGCGCGAGCCCGGAAGAGGCCTGTCAGCAAGCGGTGCAGCGCATCGC
CCGAAAACTCGGTGACTATGCCCAGTTCCAGGTGGGATTTTTGGCATTGAACAAGGCCGGTGAATACGGCGCCTACTGCA
TCCAGAGTGGTTTCAATTACGCGGTGCGCCAGCAATCGGGTGCAACCCTGATTGACGGCAAGAGTCTGCTGGGGAGTAAT
GCATGA

Upstream 100 bases:

>100_bases
CACCAGGATTCGCACCGCCAAGGCATCACCTGAAGAAGGTGCCATCCTCTTTGCCCGCCAATGTCATGTGGGCAATTCAC
GCATACAGGAGCGTTAAGCC

Downstream 100 bases:

>100_bases
AGAAATCCGCTCTCTCTCTCGCTTTGCTGCTTGCTGGCCTTTGCTTGCCCGCTGTGACCTTTGCTGATCCCCAGCCGGGA
ACAGAAAAGGTGGCCGGAGG

Product: asparaginase

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MASRRDFLKLSSLASMIGLAAPAMALGRKVNKPIVVSTWEHGMPANEAAWQVLNAGGRALDAVEAGVRVPEADPKVRTVG
YGGYPDRDGKVTLDACIMDEHMNCGSVAFLQGIKHPISVARLVMEKTPHVMLVGEGARQFALSQGFVEEELLTAEARADW
QRWLKEQTVQTINIENHDTIGMLALDANGNLSGACTTSGAAYKMHGRVGDSPLIGAGLYVDNDVGAATATGMGELMIKTV
GCHLVVELMRQGASPEEACQQAVQRIARKLGDYAQFQVGFLALNKAGEYGAYCIQSGFNYAVRQQSGATLIDGKSLLGSN
A

Sequences:

>Translated_321_residues
MASRRDFLKLSSLASMIGLAAPAMALGRKVNKPIVVSTWEHGMPANEAAWQVLNAGGRALDAVEAGVRVPEADPKVRTVG
YGGYPDRDGKVTLDACIMDEHMNCGSVAFLQGIKHPISVARLVMEKTPHVMLVGEGARQFALSQGFVEEELLTAEARADW
QRWLKEQTVQTINIENHDTIGMLALDANGNLSGACTTSGAAYKMHGRVGDSPLIGAGLYVDNDVGAATATGMGELMIKTV
GCHLVVELMRQGASPEEACQQAVQRIARKLGDYAQFQVGFLALNKAGEYGAYCIQSGFNYAVRQQSGATLIDGKSLLGSN
A
>Mature_320_residues
ASRRDFLKLSSLASMIGLAAPAMALGRKVNKPIVVSTWEHGMPANEAAWQVLNAGGRALDAVEAGVRVPEADPKVRTVGY
GGYPDRDGKVTLDACIMDEHMNCGSVAFLQGIKHPISVARLVMEKTPHVMLVGEGARQFALSQGFVEEELLTAEARADWQ
RWLKEQTVQTINIENHDTIGMLALDANGNLSGACTTSGAAYKMHGRVGDSPLIGAGLYVDNDVGAATATGMGELMIKTVG
CHLVVELMRQGASPEEACQQAVQRIARKLGDYAQFQVGFLALNKAGEYGAYCIQSGFNYAVRQQSGATLIDGKSLLGSNA

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI285002251, Length=294, Percent_Identity=39.4557823129252, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI285002253, Length=294, Percent_Identity=37.4149659863946, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI145275202, Length=257, Percent_Identity=36.9649805447471, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI145275200, Length=257, Percent_Identity=36.9649805447471, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI98991769, Length=236, Percent_Identity=31.3559322033898, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1787050, Length=237, Percent_Identity=36.7088607594937, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17569239, Length=329, Percent_Identity=33.1306990881459, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI221330102, Length=327, Percent_Identity=38.5321100917431, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24652135, Length=326, Percent_Identity=38.3435582822086, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24655553, Length=303, Percent_Identity=33.6633663366337, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24657358, Length=295, Percent_Identity=33.8983050847458, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI18921183, Length=254, Percent_Identity=31.496062992126, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21357893, Length=230, Percent_Identity=29.1304347826087, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 34167; Mature: 34036

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRRDFLKLSSLASMIGLAAPAMALGRKVNKPIVVSTWEHGMPANEAAWQVLNAGGRAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCHH
DAVEAGVRVPEADPKVRTVGYGGYPDRDGKVTLDACIMDEHMNCGSVAFLQGIKHPISVA
HHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCHHHHH
RLVMEKTPHVMLVGEGARQFALSQGFVEEELLTAEARADWQRWLKEQTVQTINIENHDTI
HHHHHCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCE
GMLALDANGNLSGACTTSGAAYKMHGRVGDSPLIGAGLYVDNDVGAATATGMGELMIKTV
EEEEEECCCCCCEEEECCCCEEEEECCCCCCCEEECCEEEECCCCCHHHCCHHHHHHHHH
GCHLVVELMRQGASPEEACQQAVQRIARKLGDYAQFQVGFLALNKAGEYGAYCIQSGFNY
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHEEEEEEEECCCCCCHHHHHCCCCE
AVRQQSGATLIDGKSLLGSNA
EEECCCCCEEECCHHHCCCCC
>Mature Secondary Structure 
ASRRDFLKLSSLASMIGLAAPAMALGRKVNKPIVVSTWEHGMPANEAAWQVLNAGGRAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCHH
DAVEAGVRVPEADPKVRTVGYGGYPDRDGKVTLDACIMDEHMNCGSVAFLQGIKHPISVA
HHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCHHHHH
RLVMEKTPHVMLVGEGARQFALSQGFVEEELLTAEARADWQRWLKEQTVQTINIENHDTI
HHHHHCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCE
GMLALDANGNLSGACTTSGAAYKMHGRVGDSPLIGAGLYVDNDVGAATATGMGELMIKTV
EEEEEECCCCCCEEEECCCCEEEEECCCCCCCEEECCEEEECCCCCHHHCCHHHHHHHHH
GCHLVVELMRQGASPEEACQQAVQRIARKLGDYAQFQVGFLALNKAGEYGAYCIQSGFNY
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHEEEEEEEECCCCCCHHHHHCCCCE
AVRQQSGATLIDGKSLLGSNA
EEECCCCCEEECCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]