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The map label for this gene is ageIM [H]

Identifier: 119773422

GI number: 119773422

Start: 328984

End: 330546

Strand: Direct

Name: ageIM [H]

Synonym: Sama_0281

Alternate gene names: 119773422

Gene position: 328984-330546 (Clockwise)

Preceding gene: 119773421

Following gene: 119773423

Centisome position: 7.64

GC content: 46.07

Gene sequence:

>1563_bases
ATGTATCCAGAATCAATTCAAGTTGTTGATTTATTTGCTGGTCCTGGTGGTCTTGGGGAGGGCTTCTCCTCTGTTGACAG
GACTTTTCAGATTCTGGTCTCGGCTGAAATGGACCCTTTCGCTCATCAAACTCTCCGTTTGAGAGCGTTTTATCGCTTAA
TTTCTAACCATGCACCCAACGAATTGGATGATTACTTTGCGTTCTGTAATGGTGTTTCTGACAAACCTTATAGCTCGGAA
ACTGAATCATTATGGGAAGACGCTGGCGAAGAAGCGTTGCAAATTGTGTTGGGTTCTGAGGACGGGAATGCTCGTCTGGA
CAGCGCAATTAAGAAAGGTCTTAATAAAGATGCCCCATGGGTGTTGATTGGCGGGCCACCCTGTCAGGCATACTCCATGG
TTGGTCGTGCCCGAAATAAAGGGAAGAAAGATTACAGACCTGAAGAAGATAATCGTCACTTCTTGTACAAAGAGTACCTG
CGGATTATTCAGCAAAATAAGCCTACGGTCTTCGTCATGGAAAACGTTAAAGGAATTCTTTCTTCTACCGTAGGTGGAAA
GCGAGTTTTTCATGAAATTCTTGATGACTTGTCGAATCCAGACGCGGCTTTAGGTGTTTCCGGCAGCGGTGAACGTTATG
TAATTTGCTCTCTCGTTACAGATGCGCAGTACCATCATGGCGATGACGCTTCTAAGTTTGATGCTGGCCAATTTGTTATT
CGTGCTGAAGACTATGGCATTCCCCAAGCCCGGCATCGTGTGATTTTGTTGGGTATCAAGCAATCTCATTTTGAGACCGT
TATTGCCAGGGGACCATTGCCACTTCTTGAAAAAGCACCTGTGGTTTCCGTCAGAGATGTGATTGGTGATCTTCCAAAGC
TTCGCAGTACTTTGTCCCGTGGTGGAGAAGCGGCGAAAGATTGGGATTCTGTTATTAAGCGTTATCTTCAAGGGCTGCTG
TCAGAATCCCGAGGGCGGGGTGATGAGGACCTGGAGCATGAGTTTCAAAACACCTTAAATGCTGTTCGTCGGGATTTATC
TAAAGGCTCTTTGCGCTACAAATGGAATCTTAGTGAGCAAAAGACTGGCACCTTGGATTGGTGGTATGGAAAAAACGCAG
AAATGCTGAGCGTTGTTTTAAACCATGAGGCCAGAGGACACATGGATTCTGATTTAAAACGATACATGTATGCAGCTTGT
TTTGCGAAGGCCCGCGGGGTATCACCGAAGGGCCATAAAGAGTTTAATTATGCTGGGCTGGCACCTGCCCATGAAAATTG
GGAGAGCGGTAAATTTGCAGACCGATTCAGGGTTCAGGTTTATGACAAACCATCCACTACTGTGGTGAGTCACATATCCA
AGGACGGCCACTATTTTATTCACCCAGACCCGGTCCAGTGTCGAAGTTTAACCGTGCGGGAAGCTGCCAGATTGCAGACA
TTCCCGGATAACTACTTTTTCCAGGGCAACAGGACACAACAATTCCATCAGGTTGGTAATGCTGTTCCTCCTCTGTTGGC
GTACAAGATAGCCAGGATTGTGAAGGAAATTATTGAAGGATAA

Upstream 100 bases:

>100_bases
CCGTTTTGGTTGATCATGGGTACGGAACCCGTCGAAAACTACAAATTTGATGTTCTTTCAGGGTAGAATCCCGAAGTTCA
ATTTAATATAGAGAGTTTCG

Downstream 100 bases:

>100_bases
GCGCAGCTACAACATGTCCCGCATTCGGGGTGAGGATACCCGCCCCGAGATGTGGCTGAGGAAACGGATCCATGCGTTGG
GGTTTCGTTATCGGCTGCAC

Product: modification methylase (cytosine-specific methyltransferase

Products: NA

Alternate protein names: M.AgeI; Cytosine-specific methyltransferase AgeI [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MYPESIQVVDLFAGPGGLGEGFSSVDRTFQILVSAEMDPFAHQTLRLRAFYRLISNHAPNELDDYFAFCNGVSDKPYSSE
TESLWEDAGEEALQIVLGSEDGNARLDSAIKKGLNKDAPWVLIGGPPCQAYSMVGRARNKGKKDYRPEEDNRHFLYKEYL
RIIQQNKPTVFVMENVKGILSSTVGGKRVFHEILDDLSNPDAALGVSGSGERYVICSLVTDAQYHHGDDASKFDAGQFVI
RAEDYGIPQARHRVILLGIKQSHFETVIARGPLPLLEKAPVVSVRDVIGDLPKLRSTLSRGGEAAKDWDSVIKRYLQGLL
SESRGRGDEDLEHEFQNTLNAVRRDLSKGSLRYKWNLSEQKTGTLDWWYGKNAEMLSVVLNHEARGHMDSDLKRYMYAAC
FAKARGVSPKGHKEFNYAGLAPAHENWESGKFADRFRVQVYDKPSTTVVSHISKDGHYFIHPDPVQCRSLTVREAARLQT
FPDNYFFQGNRTQQFHQVGNAVPPLLAYKIARIVKEIIEG

Sequences:

>Translated_520_residues
MYPESIQVVDLFAGPGGLGEGFSSVDRTFQILVSAEMDPFAHQTLRLRAFYRLISNHAPNELDDYFAFCNGVSDKPYSSE
TESLWEDAGEEALQIVLGSEDGNARLDSAIKKGLNKDAPWVLIGGPPCQAYSMVGRARNKGKKDYRPEEDNRHFLYKEYL
RIIQQNKPTVFVMENVKGILSSTVGGKRVFHEILDDLSNPDAALGVSGSGERYVICSLVTDAQYHHGDDASKFDAGQFVI
RAEDYGIPQARHRVILLGIKQSHFETVIARGPLPLLEKAPVVSVRDVIGDLPKLRSTLSRGGEAAKDWDSVIKRYLQGLL
SESRGRGDEDLEHEFQNTLNAVRRDLSKGSLRYKWNLSEQKTGTLDWWYGKNAEMLSVVLNHEARGHMDSDLKRYMYAAC
FAKARGVSPKGHKEFNYAGLAPAHENWESGKFADRFRVQVYDKPSTTVVSHISKDGHYFIHPDPVQCRSLTVREAARLQT
FPDNYFFQGNRTQQFHQVGNAVPPLLAYKIARIVKEIIEG
>Mature_520_residues
MYPESIQVVDLFAGPGGLGEGFSSVDRTFQILVSAEMDPFAHQTLRLRAFYRLISNHAPNELDDYFAFCNGVSDKPYSSE
TESLWEDAGEEALQIVLGSEDGNARLDSAIKKGLNKDAPWVLIGGPPCQAYSMVGRARNKGKKDYRPEEDNRHFLYKEYL
RIIQQNKPTVFVMENVKGILSSTVGGKRVFHEILDDLSNPDAALGVSGSGERYVICSLVTDAQYHHGDDASKFDAGQFVI
RAEDYGIPQARHRVILLGIKQSHFETVIARGPLPLLEKAPVVSVRDVIGDLPKLRSTLSRGGEAAKDWDSVIKRYLQGLL
SESRGRGDEDLEHEFQNTLNAVRRDLSKGSLRYKWNLSEQKTGTLDWWYGKNAEMLSVVLNHEARGHMDSDLKRYMYAAC
FAKARGVSPKGHKEFNYAGLAPAHENWESGKFADRFRVQVYDKPSTTVVSHISKDGHYFIHPDPVQCRSLTVREAARLQT
FPDNYFFQGNRTQQFHQVGNAVPPLLAYKIARIVKEIIEG

Specific function: This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=331, Percent_Identity=23.2628398791541, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 58415; Mature: 58415

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPESIQVVDLFAGPGGLGEGFSSVDRTFQILVSAEMDPFAHQTLRLRAFYRLISNHAPN
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCC
ELDDYFAFCNGVSDKPYSSETESLWEDAGEEALQIVLGSEDGNARLDSAIKKGLNKDAPW
HHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHCCCCCCCE
VLIGGPPCQAYSMVGRARNKGKKDYRPEEDNRHFLYKEYLRIIQQNKPTVFVMENVKGIL
EEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHH
SSTVGGKRVFHEILDDLSNPDAALGVSGSGERYVICSLVTDAQYHHGDDASKFDAGQFVI
HHCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEE
RAEDYGIPQARHRVILLGIKQSHFETVIARGPLPLLEKAPVVSVRDVIGDLPKLRSTLSR
EECCCCCCHHCCEEEEEEECHHHHHHHHHCCCCCHHHCCCCHHHHHHHCCHHHHHHHHHC
GGEAAKDWDSVIKRYLQGLLSESRGRGDEDLEHEFQNTLNAVRRDLSKGSLRYKWNLSEQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
KTGTLDWWYGKNAEMLSVVLNHEARGHMDSDLKRYMYAACFAKARGVSPKGHKEFNYAGL
CCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
APAHENWESGKFADRFRVQVYDKPSTTVVSHISKDGHYFIHPDPVQCRSLTVREAARLQT
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCC
FPDNYFFQGNRTQQFHQVGNAVPPLLAYKIARIVKEIIEG
CCCCCEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYPESIQVVDLFAGPGGLGEGFSSVDRTFQILVSAEMDPFAHQTLRLRAFYRLISNHAPN
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCC
ELDDYFAFCNGVSDKPYSSETESLWEDAGEEALQIVLGSEDGNARLDSAIKKGLNKDAPW
HHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHCCCCCCCE
VLIGGPPCQAYSMVGRARNKGKKDYRPEEDNRHFLYKEYLRIIQQNKPTVFVMENVKGIL
EEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHH
SSTVGGKRVFHEILDDLSNPDAALGVSGSGERYVICSLVTDAQYHHGDDASKFDAGQFVI
HHCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEE
RAEDYGIPQARHRVILLGIKQSHFETVIARGPLPLLEKAPVVSVRDVIGDLPKLRSTLSR
EECCCCCCHHCCEEEEEEECHHHHHHHHHCCCCCHHHCCCCHHHHHHHCCHHHHHHHHHC
GGEAAKDWDSVIKRYLQGLLSESRGRGDEDLEHEFQNTLNAVRRDLSKGSLRYKWNLSEQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
KTGTLDWWYGKNAEMLSVVLNHEARGHMDSDLKRYMYAACFAKARGVSPKGHKEFNYAGL
CCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
APAHENWESGKFADRFRVQVYDKPSTTVVSHISKDGHYFIHPDPVQCRSLTVREAARLQT
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCC
FPDNYFFQGNRTQQFHQVGNAVPPLLAYKIARIVKEIIEG
CCCCCEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8901102 [H]