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The map label for this gene is potB [H]

Identifier: 119717451

GI number: 119717451

Start: 3428255

End: 3429127

Strand: Reverse

Name: potB [H]

Synonym: Noca_3227

Alternate gene names: 119717451

Gene position: 3429127-3428255 (Counterclockwise)

Preceding gene: 119717452

Following gene: 119717450

Centisome position: 68.78

GC content: 67.12

Gene sequence:

>873_bases
GTGGGTCCGCAGCCGGTCGCGGAGTCGGGCCGGCGCCGCGGGTGGGGCTGGCTGCTGCTGGTCCCGGGCATGCTGTGGCT
GGCCGTGTTCTTCCTCTTCCCCACCGTCCAGCTCTTCTTCACCTCGCTCTACGACCCGAGCGGCTCCTACGAGCAGGGCT
ACGCGATGACCTGGCACGTGGCCAACTACGCCGACGCCGTCAGGGAGACGTGGAACCTCTTCGCGCGGTCCTTCCTGTAC
GCCGGCATCGCGACTCTCGTCTGCCTGACCTTGAGCTACCCGCTGGCCTACGCCATCGCGTTCAGGGCGGGCCGCTGGCG
CAACGTCATGCTGGTCCTGGTGATCGCGCCGTTCTTCACCAGCTTCCTGCTGCGGACGCTGTCGTGGCGCACCATCCTGT
CCGACCAGGGTCCGGTCACCAGCTTCTTGCAGACCATCTGGGTGCTGCCCGACGACGGGCGGCTGCTGGCGACGCCGGTC
GCGGTGATCGCGGGCCTGATCTACAACTTCATGCCGTTCATGACGCTGCCGCTCTACGCCTCGCTGAGCAAGGTGGACTA
CCGGCTGATCGAGGCTGCCGGCGACCTGTACTCGAGCCCCTTCACCGGCTTCCGGAAGGTGACCTTCCCACTGTCGTTGC
CCGGTGTCGTCTCCGGCACGCTGCTCACCTTCATCCCGGCCAGCGGCGACTACATCAACGCCGAGCTGCTGGGGACCCCG
AAGCAGTACATGGTCGGCAACCGGATCCAGGCGGCGTTCCTCACCGAGCACAACTACCCGCTGGCGGCCAGCCTGTCGCT
GACCTTGATGGCGATCGTCGTCGTGATGGTCGTCGTCTATGTCCGGCGGGCCGGCACCGAGGAGCTGGTCTGA

Upstream 100 bases:

>100_bases
TGTGGGCGCCCGAGCACACGTTCCTGCTCGACGCCGATCAGGATGCCCAGGCCGGCCTGATGACGCCGGAGGACAAGGAG
TGAGCGCAGCGCCGGCACCC

Downstream 100 bases:

>100_bases
TGGCCACGAGCACGCAGCTGCCCGCCCCCAGCCGCACCAGGCGCAGCGGCCGCGACCGGCCGCCGGTCGGCCGCTGGCTG
GCCGATCACATGGTCCTGTT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MGPQPVAESGRRRGWGWLLLVPGMLWLAVFFLFPTVQLFFTSLYDPSGSYEQGYAMTWHVANYADAVRETWNLFARSFLY
AGIATLVCLTLSYPLAYAIAFRAGRWRNVMLVLVIAPFFTSFLLRTLSWRTILSDQGPVTSFLQTIWVLPDDGRLLATPV
AVIAGLIYNFMPFMTLPLYASLSKVDYRLIEAAGDLYSSPFTGFRKVTFPLSLPGVVSGTLLTFIPASGDYINAELLGTP
KQYMVGNRIQAAFLTEHNYPLAASLSLTLMAIVVVMVVVYVRRAGTEELV

Sequences:

>Translated_290_residues
MGPQPVAESGRRRGWGWLLLVPGMLWLAVFFLFPTVQLFFTSLYDPSGSYEQGYAMTWHVANYADAVRETWNLFARSFLY
AGIATLVCLTLSYPLAYAIAFRAGRWRNVMLVLVIAPFFTSFLLRTLSWRTILSDQGPVTSFLQTIWVLPDDGRLLATPV
AVIAGLIYNFMPFMTLPLYASLSKVDYRLIEAAGDLYSSPFTGFRKVTFPLSLPGVVSGTLLTFIPASGDYINAELLGTP
KQYMVGNRIQAAFLTEHNYPLAASLSLTLMAIVVVMVVVYVRRAGTEELV
>Mature_289_residues
GPQPVAESGRRRGWGWLLLVPGMLWLAVFFLFPTVQLFFTSLYDPSGSYEQGYAMTWHVANYADAVRETWNLFARSFLYA
GIATLVCLTLSYPLAYAIAFRAGRWRNVMLVLVIAPFFTSFLLRTLSWRTILSDQGPVTSFLQTIWVLPDDGRLLATPVA
VIAGLIYNFMPFMTLPLYASLSKVDYRLIEAAGDLYSSPFTGFRKVTFPLSLPGVVSGTLLTFIPASGDYINAELLGTPK
QYMVGNRIQAAFLTEHNYPLAASLSLTLMAIVVVMVVVYVRRAGTEELV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=212, Percent_Identity=35.8490566037736, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1787369, Length=259, Percent_Identity=35.5212355212355, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1787713, Length=231, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32287; Mature: 32156

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPQPVAESGRRRGWGWLLLVPGMLWLAVFFLFPTVQLFFTSLYDPSGSYEQGYAMTWHV
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEH
ANYADAVRETWNLFARSFLYAGIATLVCLTLSYPLAYAIAFRAGRWRNVMLVLVIAPFFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SFLLRTLSWRTILSDQGPVTSFLQTIWVLPDDGRLLATPVAVIAGLIYNFMPFMTLPLYA
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
SLSKVDYRLIEAAGDLYSSPFTGFRKVTFPLSLPGVVSGTLLTFIPASGDYINAELLGTP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCHHHEEECCCCCEEEHEEECCC
KQYMVGNRIQAAFLTEHNYPLAASLSLTLMAIVVVMVVVYVRRAGTEELV
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GPQPVAESGRRRGWGWLLLVPGMLWLAVFFLFPTVQLFFTSLYDPSGSYEQGYAMTWHV
CCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEH
ANYADAVRETWNLFARSFLYAGIATLVCLTLSYPLAYAIAFRAGRWRNVMLVLVIAPFFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SFLLRTLSWRTILSDQGPVTSFLQTIWVLPDDGRLLATPVAVIAGLIYNFMPFMTLPLYA
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
SLSKVDYRLIEAAGDLYSSPFTGFRKVTFPLSLPGVVSGTLLTFIPASGDYINAELLGTP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCHHHEEECCCCCEEEHEEECCC
KQYMVGNRIQAAFLTEHNYPLAASLSLTLMAIVVVMVVVYVRRAGTEELV
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]