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The map label for this gene is ptsI [H]

Identifier: 119717405

GI number: 119717405

Start: 3379234

End: 3380976

Strand: Reverse

Name: ptsI [H]

Synonym: Noca_3181

Alternate gene names: 119717405

Gene position: 3380976-3379234 (Counterclockwise)

Preceding gene: 119717410

Following gene: 119717404

Centisome position: 67.81

GC content: 74.07

Gene sequence:

>1743_bases
GTGGTTTTACGCCCGAATGTGTTGACTGTCAAGGGCGGCGGTCGTTTACTGGTGACCATGGTCACCAGTCCTGAGGCTCG
TTCCACGCTGCGCGGCACCCCGGTCGTTCCTGCCGTTGCCTACGGGCCCGCCGTGCTCGTTCGCTCCGAGGTCTCGCCGG
ACGCGATCGCCCGGTTCGACCCCTCCGGCCTCGATCCCGAGACCGCCCTGCAGACCTACGAGAGCGCCGCCCAGTCGGTC
TCCGACGGGTTCGCGCGCAAGGCCGAGAAGGCCTCGGGGGCGGCGGCAGAGGTGCTCACCGCGAGCGCCGGCCTGGCCCG
CGACAAAGGGCTACGGGGCGCGGTGCGCAAGCACCTGTCCGCGGGGGACGACCTGCTCGCGGCGGTCCACGCCGGCGTCG
AGCAGTTCGTCACCGTGTTCACCGGCATGGGCGGGCTGATGGCCGAGCGGGTCACCGACCTGCGCGACATCGAGCGCCGG
ATCGTGGCCCGGCTGGTGGGCGAGCCCGAGCCCGGTGTGGTCGTGCCCGAGACGCCCGCAGTGCTGCTTGCCGAGGACCT
CGCGCCCTCTGATACCGCCGGTCTCCATCCGGCGGTGGTGCTCGGCCTGGTGACCGAGCGCGGTGGGCCGACCAGCCACA
CCGCGATCATCGCCCGCCAGCTCGGCATCCCCTGCGTCGTCGGGGTCCACGGGGCGATGGCCGTCCCGGCCGGGACCCCG
CTGCTCCTCGACGGGGTCGCCGGCACCGTCGAGCTCGGTCCCGACCCCGCCACGGCGCAGGAGCGGGTCGCCGCGGACGC
CGCCCTGCGCGCCGCTCTCGCCTCGTGGTCCGGGCCGGGCGGGACCGCCGACGGCGTACCGGTGAAGATCCTCGCGAACG
TCGCCGACGGTGAGTCCGCCCGCGCCGCGAGCAGCGCGCCCGTGCAAGGGGTCGGGCTCTTCCGCACCGAGCTGTGCTTC
CTCAACCGCCAGGACGAGCCGTCGGTCGAGGAGCAGGCGAGCATCTACGGCGAGGTGCTCGGCGCCTTCGGCAACGAGCG
GTACGTCGTGGTCCGCACGCTGGACGCCGGCTCGGACAAGCCGGTCGCGTTCGCCACCCATGTCGGCGAGGAGAACCCCG
CGCTGGGCGTGCGCGGGCTGCGGCTGTCCTTCGACAACCCGGGCCTGCTCGACCGCCAGCTCGAGGGCATCGCCAAGGCG
GCCGCGGCCACCGGCACCGAGGCGTGGGTGATGGCGCCGATGGTGGCCACCGTCGCCGAGGCCGCGGAGTTCTCGACCAA
GGTCCGCGCCCACGGGCTGAAGGCCGGCGTGATGATCGAGGTCCCGAGTGCCGCCCTGCTGGCGCACCGGGTGCTCGAGG
TCGTCGACTTCCTCTCGATCGGCACCAACGACCTGACCCAGTACACGATGGCCGCCGACCGGATGGCCACCGACCTGGCG
CACCTCACCGACCCCTGGCAGCCGGCCGTGCTGCAGCTGATCGCGATCACCGCCGAGGCCGGCTTCCACGCCGGCAAGCC
GGTCGGCGTCTGCGGCGAGGCCGCCGCCGACCCGCTGCTGGCGTGTGTCCTGGTCGGGATGGGCGTCACCTCGCTGTCGA
TGGCGGCGGCCGCCGTGCGCCCCGTCGGCGCCCGCCTCGCGACGGTCCCCATGGACCTGTGCGAGGACGCCGCCGAGGTC
GCCCTCGCCGCCACCGACCCGGCCTCCGCCCGCGACGCGGTGCGCACGCTGCTGCTGGGCTGA

Upstream 100 bases:

>100_bases
GCGACGGACAGGCGCCCGTGCTCGGCGACGAGCTGGGCCATGGCCTGTTGCCGCTCTTCGGCGTACATTCTCGGTACCGC
TCCTCTCGATCTTTGTATCT

Downstream 100 bases:

>100_bases
GGACGGTTTCCCCGGTTCACCGGGGATGTTGGAACTTCTGGCCCGAAGTTTCGCCCCAACAGTTCCGGTTTCCCCGGTTC
ACCGGGGAAACCGACCCCCC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MVLRPNVLTVKGGGRLLVTMVTSPEARSTLRGTPVVPAVAYGPAVLVRSEVSPDAIARFDPSGLDPETALQTYESAAQSV
SDGFARKAEKASGAAAEVLTASAGLARDKGLRGAVRKHLSAGDDLLAAVHAGVEQFVTVFTGMGGLMAERVTDLRDIERR
IVARLVGEPEPGVVVPETPAVLLAEDLAPSDTAGLHPAVVLGLVTERGGPTSHTAIIARQLGIPCVVGVHGAMAVPAGTP
LLLDGVAGTVELGPDPATAQERVAADAALRAALASWSGPGGTADGVPVKILANVADGESARAASSAPVQGVGLFRTELCF
LNRQDEPSVEEQASIYGEVLGAFGNERYVVVRTLDAGSDKPVAFATHVGEENPALGVRGLRLSFDNPGLLDRQLEGIAKA
AAATGTEAWVMAPMVATVAEAAEFSTKVRAHGLKAGVMIEVPSAALLAHRVLEVVDFLSIGTNDLTQYTMAADRMATDLA
HLTDPWQPAVLQLIAITAEAGFHAGKPVGVCGEAAADPLLACVLVGMGVTSLSMAAAAVRPVGARLATVPMDLCEDAAEV
ALAATDPASARDAVRTLLLG

Sequences:

>Translated_580_residues
MVLRPNVLTVKGGGRLLVTMVTSPEARSTLRGTPVVPAVAYGPAVLVRSEVSPDAIARFDPSGLDPETALQTYESAAQSV
SDGFARKAEKASGAAAEVLTASAGLARDKGLRGAVRKHLSAGDDLLAAVHAGVEQFVTVFTGMGGLMAERVTDLRDIERR
IVARLVGEPEPGVVVPETPAVLLAEDLAPSDTAGLHPAVVLGLVTERGGPTSHTAIIARQLGIPCVVGVHGAMAVPAGTP
LLLDGVAGTVELGPDPATAQERVAADAALRAALASWSGPGGTADGVPVKILANVADGESARAASSAPVQGVGLFRTELCF
LNRQDEPSVEEQASIYGEVLGAFGNERYVVVRTLDAGSDKPVAFATHVGEENPALGVRGLRLSFDNPGLLDRQLEGIAKA
AAATGTEAWVMAPMVATVAEAAEFSTKVRAHGLKAGVMIEVPSAALLAHRVLEVVDFLSIGTNDLTQYTMAADRMATDLA
HLTDPWQPAVLQLIAITAEAGFHAGKPVGVCGEAAADPLLACVLVGMGVTSLSMAAAAVRPVGARLATVPMDLCEDAAEV
ALAATDPASARDAVRTLLLG
>Mature_580_residues
MVLRPNVLTVKGGGRLLVTMVTSPEARSTLRGTPVVPAVAYGPAVLVRSEVSPDAIARFDPSGLDPETALQTYESAAQSV
SDGFARKAEKASGAAAEVLTASAGLARDKGLRGAVRKHLSAGDDLLAAVHAGVEQFVTVFTGMGGLMAERVTDLRDIERR
IVARLVGEPEPGVVVPETPAVLLAEDLAPSDTAGLHPAVVLGLVTERGGPTSHTAIIARQLGIPCVVGVHGAMAVPAGTP
LLLDGVAGTVELGPDPATAQERVAADAALRAALASWSGPGGTADGVPVKILANVADGESARAASSAPVQGVGLFRTELCF
LNRQDEPSVEEQASIYGEVLGAFGNERYVVVRTLDAGSDKPVAFATHVGEENPALGVRGLRLSFDNPGLLDRQLEGIAKA
AAATGTEAWVMAPMVATVAEAAEFSTKVRAHGLKAGVMIEVPSAALLAHRVLEVVDFLSIGTNDLTQYTMAADRMATDLA
HLTDPWQPAVLQLIAITAEAGFHAGKPVGVCGEAAADPLLACVLVGMGVTSLSMAAAAVRPVGARLATVPMDLCEDAAEV
ALAATDPASARDAVRTLLLG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=468, Percent_Identity=35.4700854700855, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI48994992, Length=496, Percent_Identity=32.8629032258064, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1789193, Length=539, Percent_Identity=30.6122448979592, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1788726, Length=553, Percent_Identity=30.0180831826401, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1787994, Length=383, Percent_Identity=31.331592689295, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 59229; Mature: 59229

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRPNVLTVKGGGRLLVTMVTSPEARSTLRGTPVVPAVAYGPAVLVRSEVSPDAIARFD
CEECCCEEEEECCCEEEEEEECCCCHHHHCCCCCCCCCHHCCCEEEEECCCCCHHHHCCC
PSGLDPETALQTYESAAQSVSDGFARKAEKASGAAAEVLTASAGLARDKGLRGAVRKHLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCCHHHHHHHHHC
AGDDLLAAVHAGVEQFVTVFTGMGGLMAERVTDLRDIERRIVARLVGEPEPGVVVPETPA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCC
VLLAEDLAPSDTAGLHPAVVLGLVTERGGPTSHTAIIARQLGIPCVVGVHGAMAVPAGTP
EEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCEECCCCCC
LLLDGVAGTVELGPDPATAQERVAADAALRAALASWSGPGGTADGVPVKILANVADGESA
EEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCC
RAASSAPVQGVGLFRTELCFLNRQDEPSVEEQASIYGEVLGAFGNERYVVVRTLDAGSDK
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
PVAFATHVGEENPALGVRGLRLSFDNPGLLDRQLEGIAKAAAATGTEAWVMAPMVATVAE
CEEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AAEFSTKVRAHGLKAGVMIEVPSAALLAHRVLEVVDFLSIGTNDLTQYTMAADRMATDLA
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HLTDPWQPAVLQLIAITAEAGFHAGKPVGVCGEAAADPLLACVLVGMGVTSLSMAAAAVR
HHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
PVGARLATVPMDLCEDAAEVALAATDPASARDAVRTLLLG
HHCCHHHHCCHHHHHHHHHEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVLRPNVLTVKGGGRLLVTMVTSPEARSTLRGTPVVPAVAYGPAVLVRSEVSPDAIARFD
CEECCCEEEEECCCEEEEEEECCCCHHHHCCCCCCCCCHHCCCEEEEECCCCCHHHHCCC
PSGLDPETALQTYESAAQSVSDGFARKAEKASGAAAEVLTASAGLARDKGLRGAVRKHLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCCHHHHHHHHHC
AGDDLLAAVHAGVEQFVTVFTGMGGLMAERVTDLRDIERRIVARLVGEPEPGVVVPETPA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCC
VLLAEDLAPSDTAGLHPAVVLGLVTERGGPTSHTAIIARQLGIPCVVGVHGAMAVPAGTP
EEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCEECCCCCC
LLLDGVAGTVELGPDPATAQERVAADAALRAALASWSGPGGTADGVPVKILANVADGESA
EEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCC
RAASSAPVQGVGLFRTELCFLNRQDEPSVEEQASIYGEVLGAFGNERYVVVRTLDAGSDK
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
PVAFATHVGEENPALGVRGLRLSFDNPGLLDRQLEGIAKAAAATGTEAWVMAPMVATVAE
CEEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AAEFSTKVRAHGLKAGVMIEVPSAALLAHRVLEVVDFLSIGTNDLTQYTMAADRMATDLA
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HLTDPWQPAVLQLIAITAEAGFHAGKPVGVCGEAAADPLLACVLVGMGVTSLSMAAAAVR
HHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
PVGARLATVPMDLCEDAAEVALAATDPASARDAVRTLLLG
HHCCHHHHCCHHHHHHHHHEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]