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The map label for this gene is hisF

Identifier: 119717263

GI number: 119717263

Start: 3237797

End: 3238573

Strand: Reverse

Name: hisF

Synonym: Noca_3039

Alternate gene names: 119717263

Gene position: 3238573-3237797 (Counterclockwise)

Preceding gene: 119717264

Following gene: 119717258

Centisome position: 64.96

GC content: 72.97

Gene sequence:

>777_bases
ATGACGCTCGCCGTACGCGTCATCCCGTGCCTGGACGTGGACGCCGGGCGGGTGGTCAAGGGCATCAACTTCAAGGAGCT
CCGCGACGCCGGCGACCCCGTGGAGCTGGCGCGCACCTACGACGCCGAGGGCGCCGACGAGCTGACCTTCCTCGACATCT
CAGCCTCCTTCGAGGGGCGGGCGACCACGATGGAGATCGTCTCGCGCACGGCCGAGGAGGTGTTCATCCCGCTGACGGTC
GGTGGGGGAGTGTCCTCGGTGGCCGACGTGGACCGGCTGCTGCGCGCGGGCGCCGACAAGGTCGCGGTCAACACCGCCGC
GATCAACCGACCGGAGCTGGTGGCCGAGATCGCCGACCGCTTCGGCAACCAGGTGCTGGTGCTCTCGGTCGATGCAAGGA
GGGTGGACGCCCGTCGCGCCCCGGGCACGGCCTCGGGCTTCGAGGTGACCACCCACGGTGGCCGGAAGTCGGCGGGCCTC
GACGCCGTCGAGTGGGCCGTGCGCGCCGCGGACCTGGGCGCGGGGGAGATCCTGCTCAACGCGATGGACGCCGACGGGAC
GCAGGACGGCTTCGACCTGGAGCTGATCCGGGCCGTACGCCGGGAGGTCACGGTCCCGGTCATCGCGTCCGGCGGGGCGG
GCGCGGTCGAGCACTTCCCGCCGGCGGTCGAGGCCGGCGCCGACGCCGTGCTGGCGGCCACCGTCTTCCACTTCGGCACG
TTGCGGATCGCCGACGTCAAGGCGTCGCTGGCGGGGGCGGGCTACCCGGTCCGCTGA

Upstream 100 bases:

>100_bases
AGCTGGTCCCGGTCGGGGTCGAGGGCGCGATCGCCGGCACCGCGCTCTACGAGGGCCGGTTCACGCTCGAGGACGCGCTG
GCGCTGACGCGCGGTGGCCG

Downstream 100 bases:

>100_bases
GCCGGTCTTCCGGCTCTCCTGACACGTCCGGGGGTACAGCGGTGTCCTGGCAACCGAACCAGCCACGCGAACGGTTGCGC
ACGCACCGCCGGCCAGGGGG

Product: imidazole glycerol phosphate synthase subunit hisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTLAVRVIPCLDVDAGRVVKGINFKELRDAGDPVELARTYDAEGADELTFLDISASFEGRATTMEIVSRTAEEVFIPLTV
GGGVSSVADVDRLLRAGADKVAVNTAAINRPELVAEIADRFGNQVLVLSVDARRVDARRAPGTASGFEVTTHGGRKSAGL
DAVEWAVRAADLGAGEILLNAMDADGTQDGFDLELIRAVRREVTVPVIASGGAGAVEHFPPAVEAGADAVLAATVFHFGT
LRIADVKASLAGAGYPVR

Sequences:

>Translated_258_residues
MTLAVRVIPCLDVDAGRVVKGINFKELRDAGDPVELARTYDAEGADELTFLDISASFEGRATTMEIVSRTAEEVFIPLTV
GGGVSSVADVDRLLRAGADKVAVNTAAINRPELVAEIADRFGNQVLVLSVDARRVDARRAPGTASGFEVTTHGGRKSAGL
DAVEWAVRAADLGAGEILLNAMDADGTQDGFDLELIRAVRREVTVPVIASGGAGAVEHFPPAVEAGADAVLAATVFHFGT
LRIADVKASLAGAGYPVR
>Mature_257_residues
TLAVRVIPCLDVDAGRVVKGINFKELRDAGDPVELARTYDAEGADELTFLDISASFEGRATTMEIVSRTAEEVFIPLTVG
GGVSSVADVDRLLRAGADKVAVNTAAINRPELVAEIADRFGNQVLVLSVDARRVDARRAPGTASGFEVTTHGGRKSAGLD
AVEWAVRAADLGAGEILLNAMDADGTQDGFDLELIRAVRREVTVPVIASGGAGAVEHFPPAVEAGADAVLAATVFHFGTL
RIADVKASLAGAGYPVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=261, Percent_Identity=43.2950191570881, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI87082028, Length=215, Percent_Identity=28.8372093023256, Blast_Score=74, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=38.8535031847134, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_NOCSJ (A1SL56)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924228.1
- ProteinModelPortal:   A1SL56
- SMR:   A1SL56
- STRING:   A1SL56
- GeneID:   4596487
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3039
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   A1SL56
- BioCyc:   NSP35761:NOCA_3039-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26865; Mature: 26733

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAVRVIPCLDVDAGRVVKGINFKELRDAGDPVELARTYDAEGADELTFLDISASFEGR
CEEEEEEEEEECCCCCCEEECCCHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCC
ATTMEIVSRTAEEVFIPLTVGGGVSSVADVDRLLRAGADKVAVNTAAINRPELVAEIADR
HHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEHHHCCCHHHHHHHHHH
FGNQVLVLSVDARRVDARRAPGTASGFEVTTHGGRKSAGLDAVEWAVRAADLGAGEILLN
HCCEEEEEEECCHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEE
AMDADGTQDGFDLELIRAVRREVTVPVIASGGAGAVEHFPPAVEAGADAVLAATVFHFGT
EECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHCCCHHHHCCHHHHHHHHHHHCC
LRIADVKASLAGAGYPVR
EEEEEEHHHHCCCCCCCC
>Mature Secondary Structure 
TLAVRVIPCLDVDAGRVVKGINFKELRDAGDPVELARTYDAEGADELTFLDISASFEGR
EEEEEEEEEECCCCCCEEECCCHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCC
ATTMEIVSRTAEEVFIPLTVGGGVSSVADVDRLLRAGADKVAVNTAAINRPELVAEIADR
HHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEHHHCCCHHHHHHHHHH
FGNQVLVLSVDARRVDARRAPGTASGFEVTTHGGRKSAGLDAVEWAVRAADLGAGEILLN
HCCEEEEEEECCHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEE
AMDADGTQDGFDLELIRAVRREVTVPVIASGGAGAVEHFPPAVEAGADAVLAATVFHFGT
EECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHCCCHHHHCCHHHHHHHHHHHCC
LRIADVKASLAGAGYPVR
EEEEEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA