Click here to switch to the map view.

The map label for this gene is def1 [H]

Identifier: 119716828

GI number: 119716828

Start: 2767771

End: 2768370

Strand: Reverse

Name: def1 [H]

Synonym: Noca_2602

Alternate gene names: 119716828

Gene position: 2768370-2767771 (Counterclockwise)

Preceding gene: 119716831

Following gene: 119716825

Centisome position: 55.52

GC content: 72.0

Gene sequence:

>600_bases
ATGTCTTCCCCCGACGACCAAGGACCGCCGCACGCCCCACACGGTCCGTTGCCGGAGGGCGGCACCGCGCGGTCGATCAC
CCGCTGGGGGACGCCGGTCATGCACCGGGCGCAGCAGCCGGTCACGACGTACGACGACGAGCTCCGCGCCCTGGCCGCCG
ACATGGTCGCGACCATGTACGCCGCGGACGGAGTCGGGCTCGCCGCCTGCCAGATCGGCGTCGACCTCGCGATGTTCGTC
TTCGACTGTCCTGACGACTCCGGCGTCCACACGGTCGGCGTCGTGTGCAACCCGCAGCTGACCCTCCCGGAGGGCCGGGA
CCGCCAGCTCGACGAGTCCGAGGAGGGCTGCCTCTCTTTCCCGGGCGCGCATGTCGAGTGCGCCCGGCCCGACCAGGCCT
CCGTCACCGGCACCGGACTGGACGGCGAGCCGGTCAGCTTCTCCGGCGACGGACTGCTGGCGCGCTGCCTCCAGCACGAG
ACCGACCACACCCGGGGCACGGTCTTCGGCGATCGGCTCTCCACCAAGCTGCGCAAACGGCTGCAGAAGGCGCACGACCG
TGCGGTCGAGGACTACCCGCTGGAGTGGCCCGCAGGCTGA

Upstream 100 bases:

>100_bases
TGGCCGATGTAGGGGACATGCGGCAAATGTAACTCGGAGTTACACCCGACGCTACAGGCGGCTGCGTGCGTCGAGTCACA
CGCTGGCTACGCTGCTCCCC

Downstream 100 bases:

>100_bases
GCCGGGCCTCGCGCCGGGTCGAGATGTGTTGCCGTTTGGTCACCAACGACAGTCCTGGGCCGGTTCGGGCTGTCGTTGGT
GACCAAACCCCACGGCCTTC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSSPDDQGPPHAPHGPLPEGGTARSITRWGTPVMHRAQQPVTTYDDELRALAADMVATMYAADGVGLAACQIGVDLAMFV
FDCPDDSGVHTVGVVCNPQLTLPEGRDRQLDESEEGCLSFPGAHVECARPDQASVTGTGLDGEPVSFSGDGLLARCLQHE
TDHTRGTVFGDRLSTKLRKRLQKAHDRAVEDYPLEWPAG

Sequences:

>Translated_199_residues
MSSPDDQGPPHAPHGPLPEGGTARSITRWGTPVMHRAQQPVTTYDDELRALAADMVATMYAADGVGLAACQIGVDLAMFV
FDCPDDSGVHTVGVVCNPQLTLPEGRDRQLDESEEGCLSFPGAHVECARPDQASVTGTGLDGEPVSFSGDGLLARCLQHE
TDHTRGTVFGDRLSTKLRKRLQKAHDRAVEDYPLEWPAG
>Mature_198_residues
SSPDDQGPPHAPHGPLPEGGTARSITRWGTPVMHRAQQPVTTYDDELRALAADMVATMYAADGVGLAACQIGVDLAMFVF
DCPDDSGVHTVGVVCNPQLTLPEGRDRQLDESEEGCLSFPGAHVECARPDQASVTGTGLDGEPVSFSGDGLLARCLQHET
DHTRGTVFGDRLSTKLRKRLQKAHDRAVEDYPLEWPAG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=154, Percent_Identity=33.1168831168831, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=28.7425149700599, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21303; Mature: 21172

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPDDQGPPHAPHGPLPEGGTARSITRWGTPVMHRAQQPVTTYDDELRALAADMVATMY
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AADGVGLAACQIGVDLAMFVFDCPDDSGVHTVGVVCNPQLTLPEGRDRQLDESEEGCLSF
HCCCCCHHHHHHHHCEEEEEEECCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCEEC
PGAHVECARPDQASVTGTGLDGEPVSFSGDGLLARCLQHETDHTRGTVFGDRLSTKLRKR
CCCCEEECCCCCCCEEECCCCCCCCEECCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
LQKAHDRAVEDYPLEWPAG
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSPDDQGPPHAPHGPLPEGGTARSITRWGTPVMHRAQQPVTTYDDELRALAADMVATMY
CCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AADGVGLAACQIGVDLAMFVFDCPDDSGVHTVGVVCNPQLTLPEGRDRQLDESEEGCLSF
HCCCCCHHHHHHHHCEEEEEEECCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCEEC
PGAHVECARPDQASVTGTGLDGEPVSFSGDGLLARCLQHETDHTRGTVFGDRLSTKLRKR
CCCCEEECCCCCCCEEECCCCCCCCEECCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
LQKAHDRAVEDYPLEWPAG
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]