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The map label for this gene is pilT [H]

Identifier: 119715364

GI number: 119715364

Start: 1192909

End: 1194201

Strand: Direct

Name: pilT [H]

Synonym: Noca_1126

Alternate gene names: 119715364

Gene position: 1192909-1194201 (Clockwise)

Preceding gene: 119715363

Following gene: 119715365

Centisome position: 23.93

GC content: 73.09

Gene sequence:

>1293_bases
TTGAACGACGCACCGACGAACGGCTTCGGCCTGCCGCTGGCCGCTCGGCGCCCGACCGCGCCCGCGGACCCGTCGGCCCC
TGCCCCCGCGCCGGTGGCCGTCCCGGTCGCCGCTCCCGTGGCCGTCCCGGCGCCGGTCGTCGCCCCGGTGGCCGCGGCCG
CCGTCGTACCTCCCGCCGCTCCCGCGCCCGCCGGATCGGCCAACCTGAGCCTCGACGACCTGCTCATCCACGTGCTGCGG
GTGGGGGCCTCCGACCTGCACCTGACCACCGGCGCGCCGCCGACCGTGCGGCTGCGCGGCGAGATGGAGGCCATCGAGGG
CTACCCCACGCTGGACGGCGAGCAGCTGCGGCGCACGCTGTACGGCGTGATGACCGAGCGGCAGCGCAAGGTCTTCGAGG
AGGAGCTCGAGCTCGACTTCGCGTACGCCGTGCCGGGCCACGCCCGGTTCCGTGTCAACGTCTTCCAGCAGCGCGAGCAC
CTCGGCGCCGTGATGCGGATGATCCCCTGGGAGATCGTCCCGCTCGAGGACCTGGGCATGCCCGAGGCGGTCGCCGGCTT
CAGCGACCTCAAGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGGTCCGGCAAGTCGACGACCCTGGCCGCGATCATCG
ACCGGATCAACCGGACCCGCCGCGGTCACATCGTGACCATCGAGGACCCGATCGAGTTCCTGCACGAGCACCGCGGCTGC
CTGGTCAACCAGCGCGAGGTCGGCTCCGACACCCACGGGTTCCGCGACGCCCTCAAGCACGTGCTGCGTCAGGACCCCGA
CGTGATCCTGGTCGGCGAGCTCCGCGACCTGGACACGATCTCGGTGGCGCTGACCGCCGCCGAGACCGGCCACCTGGTGT
TCGCGACGCTGCACACGCAGTCCGCGCAGGACACCATCACCCGCGTCGTCGACGTGTTCCCCGCCGAGCAGCAGCAGCAG
GTCCGCACCCAGCTCGCCGCGACGCTCCAGGGCGTCGTGTGCCAGACCCTCGTCAAGACCGCCGACGACCAGGGCCGCGC
CGCCGCCGTCGAGGTGATGGTCTGCAACTCCGGCATCCGGGCGATGATCCGCGACGACAAGCTGCAGCAGATCCAGGGCG
CGCTCCAAGCGGGCGCGCGGGACGGCATGCAGACCCTCAACGCCCACCTCGCCGCGCTGGTGAAGGCCGGCCGGATCACG
TACGCCGCGGGCCTCGAGCACAGCTCGAACCGCGCCGACTACGACACGCTCGTCGCCTCCGACGTCAAGCGCGCCCCGGC
CGGGTGGCAGTAG

Upstream 100 bases:

>100_bases
GCTCTCCTGACGAGGCTCCCCGCCAGCAGTCCTCAAGCCGACGGCCCCGTCGGCCGATGACCCTGATGACCCCTCATCAG
TCCCGCGCTCGGAGGACCTG

Downstream 100 bases:

>100_bases
CCGTGGCCAGCACGACGACGCAGTACGCCTACAAGGTCCGCGACGCCCAGGGCCGGTTCACCGAGGGCAAGGTGGAGGCC
GCCTCGGAGGCGGCTGTCGC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNDAPTNGFGLPLAARRPTAPADPSAPAPAPVAVPVAAPVAVPAPVVAPVAAAAVVPPAAPAPAGSANLSLDDLLIHVLR
VGASDLHLTTGAPPTVRLRGEMEAIEGYPTLDGEQLRRTLYGVMTERQRKVFEEELELDFAYAVPGHARFRVNVFQQREH
LGAVMRMIPWEIVPLEDLGMPEAVAGFSDLKRGLVLVTGPTGSGKSTTLAAIIDRINRTRRGHIVTIEDPIEFLHEHRGC
LVNQREVGSDTHGFRDALKHVLRQDPDVILVGELRDLDTISVALTAAETGHLVFATLHTQSAQDTITRVVDVFPAEQQQQ
VRTQLAATLQGVVCQTLVKTADDQGRAAAVEVMVCNSGIRAMIRDDKLQQIQGALQAGARDGMQTLNAHLAALVKAGRIT
YAAGLEHSSNRADYDTLVASDVKRAPAGWQ

Sequences:

>Translated_430_residues
MNDAPTNGFGLPLAARRPTAPADPSAPAPAPVAVPVAAPVAVPAPVVAPVAAAAVVPPAAPAPAGSANLSLDDLLIHVLR
VGASDLHLTTGAPPTVRLRGEMEAIEGYPTLDGEQLRRTLYGVMTERQRKVFEEELELDFAYAVPGHARFRVNVFQQREH
LGAVMRMIPWEIVPLEDLGMPEAVAGFSDLKRGLVLVTGPTGSGKSTTLAAIIDRINRTRRGHIVTIEDPIEFLHEHRGC
LVNQREVGSDTHGFRDALKHVLRQDPDVILVGELRDLDTISVALTAAETGHLVFATLHTQSAQDTITRVVDVFPAEQQQQ
VRTQLAATLQGVVCQTLVKTADDQGRAAAVEVMVCNSGIRAMIRDDKLQQIQGALQAGARDGMQTLNAHLAALVKAGRIT
YAAGLEHSSNRADYDTLVASDVKRAPAGWQ
>Mature_430_residues
MNDAPTNGFGLPLAARRPTAPADPSAPAPAPVAVPVAAPVAVPAPVVAPVAAAAVVPPAAPAPAGSANLSLDDLLIHVLR
VGASDLHLTTGAPPTVRLRGEMEAIEGYPTLDGEQLRRTLYGVMTERQRKVFEEELELDFAYAVPGHARFRVNVFQQREH
LGAVMRMIPWEIVPLEDLGMPEAVAGFSDLKRGLVLVTGPTGSGKSTTLAAIIDRINRTRRGHIVTIEDPIEFLHEHRGC
LVNQREVGSDTHGFRDALKHVLRQDPDVILVGELRDLDTISVALTAAETGHLVFATLHTQSAQDTITRVVDVFPAEQQQQ
VRTQLAATLQGVVCQTLVKTADDQGRAAAVEVMVCNSGIRAMIRDDKLQQIQGALQAGARDGMQTLNAHLAALVKAGRIT
YAAGLEHSSNRADYDTLVASDVKRAPAGWQ

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=40.1812688821752, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1789723, Length=191, Percent_Identity=40.3141361256544, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786296, Length=208, Percent_Identity=36.5384615384615, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 45936; Mature: 45936

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAPTNGFGLPLAARRPTAPADPSAPAPAPVAVPVAAPVAVPAPVVAPVAAAAVVPPAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCC
PAPAGSANLSLDDLLIHVLRVGASDLHLTTGAPPTVRLRGEMEAIEGYPTLDGEQLRRTL
CCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHCCCCCCCHHHHHHHH
YGVMTERQRKVFEEELELDFAYAVPGHARFRVNVFQQREHLGAVMRMIPWEIVPLEDLGM
HHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCEEECHHHCCC
PEAVAGFSDLKRGLVLVTGPTGSGKSTTLAAIIDRINRTRRGHIVTIEDPIEFLHEHRGC
CHHHHCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCC
LVNQREVGSDTHGFRDALKHVLRQDPDVILVGELRDLDTISVALTAAETGHLVFATLHTQ
EECHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEEEECC
SAQDTITRVVDVFPAEQQQQVRTQLAATLQGVVCQTLVKTADDQGRAAAVEVMVCNSGIR
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCH
AMIRDDKLQQIQGALQAGARDGMQTLNAHLAALVKAGRITYAAGLEHSSNRADYDTLVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHH
DVKRAPAGWQ
HHHCCCCCCC
>Mature Secondary Structure
MNDAPTNGFGLPLAARRPTAPADPSAPAPAPVAVPVAAPVAVPAPVVAPVAAAAVVPPAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCC
PAPAGSANLSLDDLLIHVLRVGASDLHLTTGAPPTVRLRGEMEAIEGYPTLDGEQLRRTL
CCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHCCCCCCCHHHHHHHH
YGVMTERQRKVFEEELELDFAYAVPGHARFRVNVFQQREHLGAVMRMIPWEIVPLEDLGM
HHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCEEECHHHCCC
PEAVAGFSDLKRGLVLVTGPTGSGKSTTLAAIIDRINRTRRGHIVTIEDPIEFLHEHRGC
CHHHHCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCC
LVNQREVGSDTHGFRDALKHVLRQDPDVILVGELRDLDTISVALTAAETGHLVFATLHTQ
EECHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEEEECC
SAQDTITRVVDVFPAEQQQQVRTQLAATLQGVVCQTLVKTADDQGRAAAVEVMVCNSGIR
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCH
AMIRDDKLQQIQGALQAGARDGMQTLNAHLAALVKAGRITYAAGLEHSSNRADYDTLVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHH
DVKRAPAGWQ
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]