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The map label for this gene is fumB [H]

Identifier: 119715301

GI number: 119715301

Start: 1119973

End: 1121688

Strand: Reverse

Name: fumB [H]

Synonym: Noca_1061

Alternate gene names: 119715301

Gene position: 1121688-1119973 (Counterclockwise)

Preceding gene: 119715304

Following gene: 119715300

Centisome position: 22.5

GC content: 69.58

Gene sequence:

>1716_bases
GTGGCGCAAGATCCCGAGTTCCTCTACTCCGACCTCCTGCCGACCGGCCCCGACGAGACGCCGTACCGCCTGGTCACCAC
CGAGGGCGTCTCGACGTTCGAGGCGAACGGGCGCACCTTCCTCGAGGTGTCGCCGGAGGCCATCCAGCGGCTCACCGCCG
AGGCGATGCACGACATCGCGCACTACCTGCGGCCCGCCCACCTGGCCCAGCTGCGCCGGATCATCGACGACCCGGAGGCG
TCCGGAAACGACCGGTTCGTGGCGCTCGACCTGCTCAAGAACGTCAACATCTCCGCCGGCGGCGTGCTGCCGATGTGCCA
GGACACCGGCACCGCGATCGTGATGGGCAAGAAGTCCGAGGGCGTGCTGACCGGCAGCGACGACGGCGAGGCGATCTCGC
GCGGCGTCTACGACGCGTACACCAAGCTCAACCTGCGCTACTCCCAGCTGGCGCCGCTGACGACCTACGAGGAGAAGAAC
ACCGGCACGAACCTGCCGGCCCAGATCGAGATCTACTCGACGGCCTCCCACGGGCCCGATGGGATCTCTCGGCCGGAGTA
CAAGTTCCTGTTCATGGCCAAGGGCGGGGGCTCGGCGAACAAGTCGTTCCTGTTCCAGGAGACCAAGGCCGTCCTCAACC
CGCAGCGGCTGCTGAGCTTCCTCGACGAGAAGATCCGCTCGCTCGGGACGGCCGCGTGCCCGCCGTACCACCTCGCGATC
GTGATCGGCGGCACCTCGGCGGAGTTCGCGCTCAAGACCGCGAAGTACGCCTCCGCGCACTACCTCGACAACCTGCCGAC
CTCGGGCTCGATGTCGGCGCACGGCTTCCGCGACCTCGAGCTCGAGGAGCAGGTCTTCGAGCTCACCCAGTCGTTCGGGA
TCGGCGCGCAGTTCGGCGGGAAGTACTTCTGCCACGACGTGCGGGTCGTGCGGCTGCCGCGGCACGGCGCGTCCTGCCCG
GTCGCGATCGCGGTGTCCTGCTCGGCCGACCGGCAGGCGCTGGGCAAGATCACCACCGAGGGCGTCTTCCTCGAGCAGCT
CGAGACCGATCCGGCGCAGTACATGCCCGACGCGGGCGTGGCCGAGGACATCTCCGGCGGCGAGGTGGTCGCGATCGACC
TGACCCGGCCGATGCCGGAGATCCTGGGCGAGCTGCGCAAGCACCCGGTCAAGACCCGGCTCTCGCTGACCGGCCCGCTG
GTCGTCGCCCGCGACATCGCGCACGCGAAGATCAAGGAGCGGCTCGACGCCGGCGAGGAGATGCCGGCGTACCTCCACGA
CCACCCCGTCTACTACGCCGGGCCCGCGAAGACCCCCGAGGGCATGGCGTCCGGCTCGTTCGGGCCGACCACTGCCGGCC
GGATGGACTCCTACGTCGAGCAGTTCCAGGCCGCTGGCGGCTCGATGGTCATGCTCGCCAAGGGCAACCGGTCGAAGACC
GTCACCGAGGCGTGCGCCGCGCACGGCGGGTTCTACCTCGGCTCGATCGGCGGGCCGGCCGCGCGGCTCGCGCAGGACTG
CATCAAGAGCCAGGAGGTGCTGGAGTACCCCGAGCTCGGCATGGAGGCCATCTGGAAGATCGAGGTCGAGGACTTCCCGG
CGTTCATCGTGGTCGACGACCAGGGCAACGACTTCTTCACCGACCCCTCCGGCGCCGTCACCGTGCCGCTCAGCTCGATC
GGCGCCGCCGGGATCCGGGTGCGCTCCGCGGAGTAG

Upstream 100 bases:

>100_bases
CTGGTGCCGATCGGCGTCCGAGATCCGGAGGCGGGCCGGATCGTGCTCGAGAGGTCCCTCCATGCCGGTCAGCGTAGTCG
GAGGGCTACCCTCGTGGTCC

Downstream 100 bases:

>100_bases
CGGCTGTCCTTGGTGGGACGTCATCGGGGTCGCCGTGGTGCCTCATCCGAGGTGGCTGCCCCGGCGACCGGTCGTACGAC
GCTCCCGGGCGGCCGGGCGA

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MAQDPEFLYSDLLPTGPDETPYRLVTTEGVSTFEANGRTFLEVSPEAIQRLTAEAMHDIAHYLRPAHLAQLRRIIDDPEA
SGNDRFVALDLLKNVNISAGGVLPMCQDTGTAIVMGKKSEGVLTGSDDGEAISRGVYDAYTKLNLRYSQLAPLTTYEEKN
TGTNLPAQIEIYSTASHGPDGISRPEYKFLFMAKGGGSANKSFLFQETKAVLNPQRLLSFLDEKIRSLGTAACPPYHLAI
VIGGTSAEFALKTAKYASAHYLDNLPTSGSMSAHGFRDLELEEQVFELTQSFGIGAQFGGKYFCHDVRVVRLPRHGASCP
VAIAVSCSADRQALGKITTEGVFLEQLETDPAQYMPDAGVAEDISGGEVVAIDLTRPMPEILGELRKHPVKTRLSLTGPL
VVARDIAHAKIKERLDAGEEMPAYLHDHPVYYAGPAKTPEGMASGSFGPTTAGRMDSYVEQFQAAGGSMVMLAKGNRSKT
VTEACAAHGGFYLGSIGGPAARLAQDCIKSQEVLEYPELGMEAIWKIEVEDFPAFIVVDDQGNDFFTDPSGAVTVPLSSI
GAAGIRVRSAE

Sequences:

>Translated_571_residues
MAQDPEFLYSDLLPTGPDETPYRLVTTEGVSTFEANGRTFLEVSPEAIQRLTAEAMHDIAHYLRPAHLAQLRRIIDDPEA
SGNDRFVALDLLKNVNISAGGVLPMCQDTGTAIVMGKKSEGVLTGSDDGEAISRGVYDAYTKLNLRYSQLAPLTTYEEKN
TGTNLPAQIEIYSTASHGPDGISRPEYKFLFMAKGGGSANKSFLFQETKAVLNPQRLLSFLDEKIRSLGTAACPPYHLAI
VIGGTSAEFALKTAKYASAHYLDNLPTSGSMSAHGFRDLELEEQVFELTQSFGIGAQFGGKYFCHDVRVVRLPRHGASCP
VAIAVSCSADRQALGKITTEGVFLEQLETDPAQYMPDAGVAEDISGGEVVAIDLTRPMPEILGELRKHPVKTRLSLTGPL
VVARDIAHAKIKERLDAGEEMPAYLHDHPVYYAGPAKTPEGMASGSFGPTTAGRMDSYVEQFQAAGGSMVMLAKGNRSKT
VTEACAAHGGFYLGSIGGPAARLAQDCIKSQEVLEYPELGMEAIWKIEVEDFPAFIVVDDQGNDFFTDPSGAVTVPLSSI
GAAGIRVRSAE
>Mature_570_residues
AQDPEFLYSDLLPTGPDETPYRLVTTEGVSTFEANGRTFLEVSPEAIQRLTAEAMHDIAHYLRPAHLAQLRRIIDDPEAS
GNDRFVALDLLKNVNISAGGVLPMCQDTGTAIVMGKKSEGVLTGSDDGEAISRGVYDAYTKLNLRYSQLAPLTTYEEKNT
GTNLPAQIEIYSTASHGPDGISRPEYKFLFMAKGGGSANKSFLFQETKAVLNPQRLLSFLDEKIRSLGTAACPPYHLAIV
IGGTSAEFALKTAKYASAHYLDNLPTSGSMSAHGFRDLELEEQVFELTQSFGIGAQFGGKYFCHDVRVVRLPRHGASCPV
AIAVSCSADRQALGKITTEGVFLEQLETDPAQYMPDAGVAEDISGGEVVAIDLTRPMPEILGELRKHPVKTRLSLTGPLV
VARDIAHAKIKERLDAGEEMPAYLHDHPVYYAGPAKTPEGMASGSFGPTTAGRMDSYVEQFQAAGGSMVMLAKGNRSKTV
TEACAAHGGFYLGSIGGPAARLAQDCIKSQEVLEYPELGMEAIWKIEVEDFPAFIVVDDQGNDFFTDPSGAVTVPLSSIG
AAGIRVRSAE

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=546, Percent_Identity=61.7216117216117, Blast_Score=693, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=546, Percent_Identity=61.3553113553114, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=159, Percent_Identity=28.3018867924528, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 61395; Mature: 61264

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQDPEFLYSDLLPTGPDETPYRLVTTEGVSTFEANGRTFLEVSPEAIQRLTAEAMHDIA
CCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEEECCCEEEEEECHHHHHHHHHHHHHHHH
HYLRPAHLAQLRRIIDDPEASGNDRFVALDLLKNVNISAGGVLPMCQDTGTAIVMGKKSE
HHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCCCCEEECCCCCEEEEECCCC
GVLTGSDDGEAISRGVYDAYTKLNLRYSQLAPLTTYEEKNTGTNLPAQIEIYSTASHGPD
CEEECCCCCHHHHHHHHHHHHHEEEEHHHCCCCEEECCCCCCCCCCEEEEEEEECCCCCC
GISRPEYKFLFMAKGGGSANKSFLFQETKAVLNPQRLLSFLDEKIRSLGTAACPPYHLAI
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
VIGGTSAEFALKTAKYASAHYLDNLPTSGSMSAHGFRDLELEEQVFELTQSFGIGAQFGG
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
KYFCHDVRVVRLPRHGASCPVAIAVSCSADRQALGKITTEGVFLEQLETDPAQYMPDAGV
EEEEECEEEEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEHHCCCHHHHCCCCCC
AEDISGGEVVAIDLTRPMPEILGELRKHPVKTRLSLTGPLVVARDIAHAKIKERLDAGEE
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHHHHHHCCCCC
MPAYLHDHPVYYAGPAKTPEGMASGSFGPTTAGRMDSYVEQFQAAGGSMVMLAKGNRSKT
CCHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VTEACAAHGGFYLGSIGGPAARLAQDCIKSQEVLEYPELGMEAIWKIEVEDFPAFIVVDD
HHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEC
QGNDFFTDPSGAVTVPLSSIGAAGIRVRSAE
CCCCEEECCCCEEEEEHHHCCCCCEEEECCC
>Mature Secondary Structure 
AQDPEFLYSDLLPTGPDETPYRLVTTEGVSTFEANGRTFLEVSPEAIQRLTAEAMHDIA
CCCHHHHHHHCCCCCCCCCCEEEEEECCCEEEECCCEEEEEECHHHHHHHHHHHHHHHH
HYLRPAHLAQLRRIIDDPEASGNDRFVALDLLKNVNISAGGVLPMCQDTGTAIVMGKKSE
HHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCCCCEEECCCCCEEEEECCCC
GVLTGSDDGEAISRGVYDAYTKLNLRYSQLAPLTTYEEKNTGTNLPAQIEIYSTASHGPD
CEEECCCCCHHHHHHHHHHHHHEEEEHHHCCCCEEECCCCCCCCCCEEEEEEEECCCCCC
GISRPEYKFLFMAKGGGSANKSFLFQETKAVLNPQRLLSFLDEKIRSLGTAACPPYHLAI
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
VIGGTSAEFALKTAKYASAHYLDNLPTSGSMSAHGFRDLELEEQVFELTQSFGIGAQFGG
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
KYFCHDVRVVRLPRHGASCPVAIAVSCSADRQALGKITTEGVFLEQLETDPAQYMPDAGV
EEEEECEEEEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEHHCCCHHHHCCCCCC
AEDISGGEVVAIDLTRPMPEILGELRKHPVKTRLSLTGPLVVARDIAHAKIKERLDAGEE
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHHHHHHCCCCC
MPAYLHDHPVYYAGPAKTPEGMASGSFGPTTAGRMDSYVEQFQAAGGSMVMLAKGNRSKT
CCHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VTEACAAHGGFYLGSIGGPAARLAQDCIKSQEVLEYPELGMEAIWKIEVEDFPAFIVVDD
HHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEC
QGNDFFTDPSGAVTVPLSSIGAAGIRVRSAE
CCCCEEECCCCEEEEEHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]