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The map label for this gene is dosT [H]

Identifier: 119715237

GI number: 119715237

Start: 1043105

End: 1044838

Strand: Direct

Name: dosT [H]

Synonym: Noca_0994

Alternate gene names: 119715237

Gene position: 1043105-1044838 (Clockwise)

Preceding gene: 119715235

Following gene: 119715239

Centisome position: 20.92

GC content: 73.53

Gene sequence:

>1734_bases
ATGCAGCTGCCGCGGCACGCGGGGTCGCCGGACGACCCCGTCCACCTCGGCTCGGAGCTGGCCGGGACCGGGTTCGACGA
GCTCCTGCGCGAGGTGCTGCTGCGGGTCCACGGGGTGCTCGACGACCGGGCCCGGTGGCGCCTGCTCCTCGACGCCGTGG
TCACGATGGCGGCCGACCTCTCCCTCGACGACCTGCTCACCCGCATCGTGGAGATCGCCAGCGACCTGGCCGGTGCCCGC
TACGCCGCGCTCGGAGTGCTCGGCGCCGGTGAGGACCGGCGGCTCCAGACGTTCGTCACCCACGGCGTGACCGGTGCGCA
GCACCACGACATCGGCGATCTGCCGACCGGTCACGGTCTGCTCGGCCTGATCATCGACCAGCCCGAGCCGTTGCGCCTCC
ACGACATCGCCGAGCACCCTGCGTCGTACGGCTTCCCGCCGCACCATCCGCCGATGCGGTCCTTCCTCGGCGTCCCCGTG
CGGATCCGCGACAAGGTGTTCGGGAACCTCTACCTCACCGAGAAGGTCGGCGACGGCGACTTCACCGAGCAGGACGAGGA
GATCGTCGTGGCCCTGGCCGCGGCCGCCGGCGTGGCGATCGAGAACGCCCGGCTGCACGAGGAGTCGGCACGCCGGGAGC
GGTGGCTCGCGGCGACCGCGGAGATCACGGCCCGGCTCGCCGGCCCGGGCGGGCACGAGGACGCCCTGCAGATCGTCGCC
GACCACGCGCGCGAGCTCGCCGTGGCCGACGTGGCCTGGATCGTGGCGGGGCCGGGGGTCGAGGCCCTGCGGGTGCGCGT
CGTGTCCGGCGAGAAGGCCGACCCGGTGGTGATGGACAACGTCGACCTCGAGACCTCGCTGACCAGCAGCGTCGTGCGGA
CCGCGACCCCGGTCGCCGTCGAGGACATCGGTGCGGACCCCCAGGCCGTGAACGTGCCCAAGGCGCTCGGGTGGCCGCGG
ATCGGCCCCGCGATCATCGTGCCACTGCGCGGCACGACCGCGGCCCAGGGCGTGCTCGCCCTCGGCTGGAAGCCGGAGAA
CCTGATGGCCTTCCACGCCGTCGACCCGACGCTGCCGGCCAGCTTCGCCGAGCAGGCTGCACTGGCTCTCGAGGTGACCC
AGGCCCGCGAGGACCGACAGCGGCTGGTGCTCTTCGAGGACCGCGATCGCATCGGCCGCGACCTGCACGACCTGGTGATC
CAGCGGCTGTTCGCGGTCGGGCTGAGCCTGCAGAGCAGCGCGCGCGCGGCCGGCTCGCCGGAGCTCACCGCGAAGCTGGA
CGCCGCCGTGGACGACCTCGACACGACCATCCGCGACATCCGGCGCACGATCTTCGCGCTCGGCTCGGCAGAGAACGCCT
CGGACATCCAGGCGGAGGTCACCCGGATGGTGGACCGCGCGGCAGGGACGCTGAAGTTCCGCCCCGCGCTGCGCTTCGAG
GGCCCGGTGCGCACCCGGGTCGGCACCGAGGTGGCGCCCGACGTGCTCGCCGTGCTCACCGAGGCGCTCTCCAACGCCGC
CCGGCACGCACGAGCCACCTCGGTCTCGGTCGAGCTGGCGGTCCTCGACGGGATCCGCCTGACCGTGACCGACAACGGGC
GCGGGATGCCCGAGGACGTGCCCCAGAGCGGCTTGTCGAACATGCGGCAGCGCGCCGAGCGGCGCGGCGGCCGCTGCACG
ATCACGTCACGGCCCGGTGAGGGCACGACCGTCGAGTGGTGGGTCCCCTCCTGA

Upstream 100 bases:

>100_bases
CCTCCCAGGCCGTAGCGACCCCGTCTTGCGGGACCTTGGTCCCTGGGCGGGAAGGGAGTTGATAGCGTTTCGGGAGGCCC
GAGCTGACAGGGAGGGTGGC

Downstream 100 bases:

>100_bases
CCGCGTCTCGCGTCGGCTTCGTCGCCGCGCGGGCCGCGGTGGTCGCTCGTGGGGTCGGTGCTCCGACGATGTGGTTGCGA
GGCTGCCCTTCGGGCAGATG

Product: GAF domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MQLPRHAGSPDDPVHLGSELAGTGFDELLREVLLRVHGVLDDRARWRLLLDAVVTMAADLSLDDLLTRIVEIASDLAGAR
YAALGVLGAGEDRRLQTFVTHGVTGAQHHDIGDLPTGHGLLGLIIDQPEPLRLHDIAEHPASYGFPPHHPPMRSFLGVPV
RIRDKVFGNLYLTEKVGDGDFTEQDEEIVVALAAAAGVAIENARLHEESARRERWLAATAEITARLAGPGGHEDALQIVA
DHARELAVADVAWIVAGPGVEALRVRVVSGEKADPVVMDNVDLETSLTSSVVRTATPVAVEDIGADPQAVNVPKALGWPR
IGPAIIVPLRGTTAAQGVLALGWKPENLMAFHAVDPTLPASFAEQAALALEVTQAREDRQRLVLFEDRDRIGRDLHDLVI
QRLFAVGLSLQSSARAAGSPELTAKLDAAVDDLDTTIRDIRRTIFALGSAENASDIQAEVTRMVDRAAGTLKFRPALRFE
GPVRTRVGTEVAPDVLAVLTEALSNAARHARATSVSVELAVLDGIRLTVTDNGRGMPEDVPQSGLSNMRQRAERRGGRCT
ITSRPGEGTTVEWWVPS

Sequences:

>Translated_577_residues
MQLPRHAGSPDDPVHLGSELAGTGFDELLREVLLRVHGVLDDRARWRLLLDAVVTMAADLSLDDLLTRIVEIASDLAGAR
YAALGVLGAGEDRRLQTFVTHGVTGAQHHDIGDLPTGHGLLGLIIDQPEPLRLHDIAEHPASYGFPPHHPPMRSFLGVPV
RIRDKVFGNLYLTEKVGDGDFTEQDEEIVVALAAAAGVAIENARLHEESARRERWLAATAEITARLAGPGGHEDALQIVA
DHARELAVADVAWIVAGPGVEALRVRVVSGEKADPVVMDNVDLETSLTSSVVRTATPVAVEDIGADPQAVNVPKALGWPR
IGPAIIVPLRGTTAAQGVLALGWKPENLMAFHAVDPTLPASFAEQAALALEVTQAREDRQRLVLFEDRDRIGRDLHDLVI
QRLFAVGLSLQSSARAAGSPELTAKLDAAVDDLDTTIRDIRRTIFALGSAENASDIQAEVTRMVDRAAGTLKFRPALRFE
GPVRTRVGTEVAPDVLAVLTEALSNAARHARATSVSVELAVLDGIRLTVTDNGRGMPEDVPQSGLSNMRQRAERRGGRCT
ITSRPGEGTTVEWWVPS
>Mature_577_residues
MQLPRHAGSPDDPVHLGSELAGTGFDELLREVLLRVHGVLDDRARWRLLLDAVVTMAADLSLDDLLTRIVEIASDLAGAR
YAALGVLGAGEDRRLQTFVTHGVTGAQHHDIGDLPTGHGLLGLIIDQPEPLRLHDIAEHPASYGFPPHHPPMRSFLGVPV
RIRDKVFGNLYLTEKVGDGDFTEQDEEIVVALAAAAGVAIENARLHEESARRERWLAATAEITARLAGPGGHEDALQIVA
DHARELAVADVAWIVAGPGVEALRVRVVSGEKADPVVMDNVDLETSLTSSVVRTATPVAVEDIGADPQAVNVPKALGWPR
IGPAIIVPLRGTTAAQGVLALGWKPENLMAFHAVDPTLPASFAEQAALALEVTQAREDRQRLVLFEDRDRIGRDLHDLVI
QRLFAVGLSLQSSARAAGSPELTAKLDAAVDDLDTTIRDIRRTIFALGSAENASDIQAEVTRMVDRAAGTLKFRPALRFE
GPVRTRVGTEVAPDVLAVLTEALSNAARHARATSVSVELAVLDGIRLTVTDNGRGMPEDVPQSGLSNMRQRAERRGGRCT
ITSRPGEGTTVEWWVPS

Specific function: Interacts with the two-component regulatory system devR/devS (dosR/dosS) involved in onset of the dormancy response. May act as a direct hypoxia sensor (rather than a redox sensor); the normal state is the oxygenated form, while the deoxy form is probably

COG id: COG2203

COG function: function code T; FOG: GAF domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=220, Percent_Identity=30.4545454545455, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788812, Length=210, Percent_Identity=29.0476190476191, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR011712 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 61933; Mature: 61933

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPRHAGSPDDPVHLGSELAGTGFDELLREVLLRVHGVLDDRARWRLLLDAVVTMAADL
CCCCCCCCCCCCCHHCCCHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
SLDDLLTRIVEIASDLAGARYAALGVLGAGEDRRLQTFVTHGVTGAQHHDIGDLPTGHGL
CHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
LGLIIDQPEPLRLHDIAEHPASYGFPPHHPPMRSFLGVPVRIRDKVFGNLYLTEKVGDGD
EEEEECCCCCCCHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEECHHCCCEEEEEECCCCC
FTEQDEEIVVALAAAAGVAIENARLHEESARRERWLAATAEITARLAGPGGHEDALQIVA
CCCCCCHHEEEEHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHH
DHARELAVADVAWIVAGPGVEALRVRVVSGEKADPVVMDNVDLETSLTSSVVRTATPVAV
HHHHHHHHHHEEEEEECCCHHHEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCCH
EDIGADPQAVNVPKALGWPRIGPAIIVPLRGTTAAQGVLALGWKPENLMAFHAVDPTLPA
HHCCCCCCCCCCCHHCCCCCCCCEEEEEECCCCHHCCEEEECCCCCCEEEEEECCCCCCH
SFAEQAALALEVTQAREDRQRLVLFEDRDRIGRDLHDLVIQRLFAVGLSLQSSARAAGSP
HHHHHHHHHHHHHHHHHCCCEEEEEECHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
ELTAKLDAAVDDLDTTIRDIRRTIFALGSAENASDIQAEVTRMVDRAAGTLKFRPALRFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEECCCEECC
GPVRTRVGTEVAPDVLAVLTEALSNAARHARATSVSVELAVLDGIRLTVTDNGRGMPEDV
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEEECCCCCCCHHH
PQSGLSNMRQRAERRGGRCTITSRPGEGTTVEWWVPS
HHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCC
>Mature Secondary Structure
MQLPRHAGSPDDPVHLGSELAGTGFDELLREVLLRVHGVLDDRARWRLLLDAVVTMAADL
CCCCCCCCCCCCCHHCCCHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
SLDDLLTRIVEIASDLAGARYAALGVLGAGEDRRLQTFVTHGVTGAQHHDIGDLPTGHGL
CHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
LGLIIDQPEPLRLHDIAEHPASYGFPPHHPPMRSFLGVPVRIRDKVFGNLYLTEKVGDGD
EEEEECCCCCCCHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEECHHCCCEEEEEECCCCC
FTEQDEEIVVALAAAAGVAIENARLHEESARRERWLAATAEITARLAGPGGHEDALQIVA
CCCCCCHHEEEEHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHH
DHARELAVADVAWIVAGPGVEALRVRVVSGEKADPVVMDNVDLETSLTSSVVRTATPVAV
HHHHHHHHHHEEEEEECCCHHHEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCCH
EDIGADPQAVNVPKALGWPRIGPAIIVPLRGTTAAQGVLALGWKPENLMAFHAVDPTLPA
HHCCCCCCCCCCCHHCCCCCCCCEEEEEECCCCHHCCEEEECCCCCCEEEEEECCCCCCH
SFAEQAALALEVTQAREDRQRLVLFEDRDRIGRDLHDLVIQRLFAVGLSLQSSARAAGSP
HHHHHHHHHHHHHHHHHCCCEEEEEECHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
ELTAKLDAAVDDLDTTIRDIRRTIFALGSAENASDIQAEVTRMVDRAAGTLKFRPALRFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEECCCEECC
GPVRTRVGTEVAPDVLAVLTEALSNAARHARATSVSVELAVLDGIRLTVTDNGRGMPEDV
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEEECCCCCCCHHH
PQSGLSNMRQRAERRGGRCTITSRPGEGTTVEWWVPS
HHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with a nitrogenous group as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]