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The map label for this gene is aarA [H]

Identifier: 119715133

GI number: 119715133

Start: 928401

End: 929744

Strand: Reverse

Name: aarA [H]

Synonym: Noca_0888

Alternate gene names: 119715133

Gene position: 929744-928401 (Counterclockwise)

Preceding gene: 119715140

Following gene: 119715132

Centisome position: 18.65

GC content: 66.52

Gene sequence:

>1344_bases
GTGGAGGGGAGCCGGGTTTCGCGTGCCACCACGCCGCAGTCTAAGTTGGAGCGCGTGACTGATTCTCTGACCGTCCGTGA
CAACCGCACCGGACAGGAGTACGAGGTCCCGATCACCGACGGCACCATCAAGGCCGCGGACCTGGGCCAGATCAAGGCCG
AGGAGGACGCGCCCGGCCTCGCGGTCTACGACCCCGGCTTCGTGAACACCGCCTCCTGCCGCAGCTCGGTGACCTTCATC
GACGGCGACAAGGGCGTCCTGGAGTACCGCGGCTACCCGATCGAGCAGCTGGCGGAGAAGTCGAGCTTCCTCGAGGTCGC
CTACCTCCTGGTCCACGGCTCGCTGCCGACCAGGGCGGAGTACGAGGCGTGGGTGCACGAGATCACGTACCACACGTTCG
TGCACGAGAACGTCAAGGAGTTCATGCAGGGCTTCCGCTACGACGCGCACCCGATGGGGATGCTGATGGCGTCGGTCGGG
GCCCTGTCGACGTTCTACCCCGACGCCCGCAACATCAGCGACGCCGACAACCGGCACATGCAGATCGTGCGGATGATCGC
GAAGATGCCGACGCTGGGCGCCTGGTCGTTCCGGCACGCGCAGGGCAAGCCGTTCGTCTACCCCGACAACGAGCTCGGCT
ACACCGCCAACTTCCTCTCGATGCTGTTCAAGATGAGCGAGCACCGGTTCGAGGCCGACGAGCGCCTGGTGAAGGCCCTC
GACGTGCTGTTCATCCTGCACGCCGACCACGAGCAGAACGCCTCCACCAACGCGGTCCGCTCGGTCGGCTCGACCCAGGT
CGACCCCTACTCCGCGGTCGCCGCCGGGGTCGGCGCCCTCTACGGCCCGCTGCACGGCGGCGCCAACGAGGCGGTGCTGC
GGATGCTGCGCCGGATCGGCACCAAGGAGAACATCCCCTCCTTCATCCAGGGCGTGAAGGACGGCAACGAGCGGCTGATG
GGCTTCGGCCACCGCGTCTACAAGAACTACGACCCCCGCGCCAAGATCATCAAGAAGTCCGCCGAGGACGTCTTCGAGGT
CACCGGCACCAACCCGCTGCTGGACATCGCGCTGGAGCTGGAGAAGATCGCGCTCGAGGACGAGTACTTCGTCAAGCGCC
GCCTCTACCCCAACGTGGACTTCTACTCCGGCCTGATCTACGAGGCCTTCCAGTTCCCGCCGGAGATGTTCACCGTGTTG
TTCGCGATCGGCCGCACCCCCGGCTGGCTGTCCCAGTGGCTCGAGCTGGTGCAGGACAAGGAGCAGAAGATCGCCCGCCC
CAAGCAGATCTACACCGGTGAGCGCGGCCTGGACTTCGTGCCCGCTGCCGAGCGCTGGGCGTGA

Upstream 100 bases:

>100_bases
TGCGGTCCGGATCTGCACCCCGATCCCGTGGCGGGTCGGCGCGCGGCAGGAGGCGCGACTGACAAGACTACGGCGCCCCG
CTCGGCGGTGTCGAATCACC

Downstream 100 bases:

>100_bases
GCGAAGCTCAGCCTCAGCGCTCGGCAAGCCACGGGCCAGGTCCGAGTGCGGCCGAGCCTGCGGGGCCGCGACGGAGCGCA
TCGAGCCCATGGCAATGACC

Product: citrate synthase

Products: NA

Alternate protein names: Acetic acid resistance protein [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MEGSRVSRATTPQSKLERVTDSLTVRDNRTGQEYEVPITDGTIKAADLGQIKAEEDAPGLAVYDPGFVNTASCRSSVTFI
DGDKGVLEYRGYPIEQLAEKSSFLEVAYLLVHGSLPTRAEYEAWVHEITYHTFVHENVKEFMQGFRYDAHPMGMLMASVG
ALSTFYPDARNISDADNRHMQIVRMIAKMPTLGAWSFRHAQGKPFVYPDNELGYTANFLSMLFKMSEHRFEADERLVKAL
DVLFILHADHEQNASTNAVRSVGSTQVDPYSAVAAGVGALYGPLHGGANEAVLRMLRRIGTKENIPSFIQGVKDGNERLM
GFGHRVYKNYDPRAKIIKKSAEDVFEVTGTNPLLDIALELEKIALEDEYFVKRRLYPNVDFYSGLIYEAFQFPPEMFTVL
FAIGRTPGWLSQWLELVQDKEQKIARPKQIYTGERGLDFVPAAERWA

Sequences:

>Translated_447_residues
MEGSRVSRATTPQSKLERVTDSLTVRDNRTGQEYEVPITDGTIKAADLGQIKAEEDAPGLAVYDPGFVNTASCRSSVTFI
DGDKGVLEYRGYPIEQLAEKSSFLEVAYLLVHGSLPTRAEYEAWVHEITYHTFVHENVKEFMQGFRYDAHPMGMLMASVG
ALSTFYPDARNISDADNRHMQIVRMIAKMPTLGAWSFRHAQGKPFVYPDNELGYTANFLSMLFKMSEHRFEADERLVKAL
DVLFILHADHEQNASTNAVRSVGSTQVDPYSAVAAGVGALYGPLHGGANEAVLRMLRRIGTKENIPSFIQGVKDGNERLM
GFGHRVYKNYDPRAKIIKKSAEDVFEVTGTNPLLDIALELEKIALEDEYFVKRRLYPNVDFYSGLIYEAFQFPPEMFTVL
FAIGRTPGWLSQWLELVQDKEQKIARPKQIYTGERGLDFVPAAERWA
>Mature_447_residues
MEGSRVSRATTPQSKLERVTDSLTVRDNRTGQEYEVPITDGTIKAADLGQIKAEEDAPGLAVYDPGFVNTASCRSSVTFI
DGDKGVLEYRGYPIEQLAEKSSFLEVAYLLVHGSLPTRAEYEAWVHEITYHTFVHENVKEFMQGFRYDAHPMGMLMASVG
ALSTFYPDARNISDADNRHMQIVRMIAKMPTLGAWSFRHAQGKPFVYPDNELGYTANFLSMLFKMSEHRFEADERLVKAL
DVLFILHADHEQNASTNAVRSVGSTQVDPYSAVAAGVGALYGPLHGGANEAVLRMLRRIGTKENIPSFIQGVKDGNERLM
GFGHRVYKNYDPRAKIIKKSAEDVFEVTGTNPLLDIALELEKIALEDEYFVKRRLYPNVDFYSGLIYEAFQFPPEMFTVL
FAIGRTPGWLSQWLELVQDKEQKIARPKQIYTGERGLDFVPAAERWA

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=366, Percent_Identity=27.8688524590164, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786939, Length=392, Percent_Identity=47.7040816326531, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1786527, Length=380, Percent_Identity=32.6315789473684, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17555174, Length=371, Percent_Identity=27.2237196765499, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319850, Length=362, Percent_Identity=27.3480662983425, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324328, Length=345, Percent_Identity=25.7971014492754, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=397, Percent_Identity=24.6851385390428, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356863, Length=403, Percent_Identity=27.2952853598015, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24640124, Length=402, Percent_Identity=26.6169154228856, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24640126, Length=402, Percent_Identity=26.6169154228856, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 50331; Mature: 50331

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGSRVSRATTPQSKLERVTDSLTVRDNRTGQEYEVPITDGTIKAADLGQIKAEEDAPGL
CCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCE
AVYDPGFVNTASCRSSVTFIDGDKGVLEYRGYPIEQLAEKSSFLEVAYLLVHGSLPTRAE
EEECCCCCCCHHHCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
YEAWVHEITYHTFVHENVKEFMQGFRYDAHPMGMLMASVGALSTFYPDARNISDADNRHM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QIVRMIAKMPTLGAWSFRHAQGKPFVYPDNELGYTANFLSMLFKMSEHRFEADERLVKAL
HHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
DVLFILHADHEQNASTNAVRSVGSTQVDPYSAVAAGVGALYGPLHGGANEAVLRMLRRIG
HHHHEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
TKENIPSFIQGVKDGNERLMGFGHRVYKNYDPRAKIIKKSAEDVFEVTGTNPLLDIALEL
CCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EKIALEDEYFVKRRLYPNVDFYSGLIYEAFQFPPEMFTVLFAIGRTPGWLSQWLELVQDK
HHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQKIARPKQIYTGERGLDFVPAAERWA
HHHHCCCHHHHCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MEGSRVSRATTPQSKLERVTDSLTVRDNRTGQEYEVPITDGTIKAADLGQIKAEEDAPGL
CCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCE
AVYDPGFVNTASCRSSVTFIDGDKGVLEYRGYPIEQLAEKSSFLEVAYLLVHGSLPTRAE
EEECCCCCCCHHHCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
YEAWVHEITYHTFVHENVKEFMQGFRYDAHPMGMLMASVGALSTFYPDARNISDADNRHM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QIVRMIAKMPTLGAWSFRHAQGKPFVYPDNELGYTANFLSMLFKMSEHRFEADERLVKAL
HHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
DVLFILHADHEQNASTNAVRSVGSTQVDPYSAVAAGVGALYGPLHGGANEAVLRMLRRIG
HHHHEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
TKENIPSFIQGVKDGNERLMGFGHRVYKNYDPRAKIIKKSAEDVFEVTGTNPLLDIALEL
CCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EKIALEDEYFVKRRLYPNVDFYSGLIYEAFQFPPEMFTVLFAIGRTPGWLSQWLELVQDK
HHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQKIARPKQIYTGERGLDFVPAAERWA
HHHHCCCHHHHCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156811 [H]