BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is paaF [C]

Identifier: 119715058

GI number: 119715058

Start: 854737

End: 855504

Strand: Reverse

Name: paaF [C]

Synonym: Noca_0811

Alternate gene names: 119715058

Gene position: 855504-854737 (Counterclockwise)

Preceding gene: 119715059

Following gene: 119715056

Centisome position: 17.16

GC content: 70.18

Gene sequence:

>768_bases
ATGACCGAGGAAGCGGTGCTGACGTCCCAGCGGGACGGCGTGCTGGTCGTCACCCTGAACCGGCCGAACATGCGCAACGC
GATCAACGAGGAGCTCTCGCTCGGCGTCGCGGAGGCCATGGCTCGGCTCGACCAGAGCGACGCCCTCCGCGTCGCCGTCC
TGCACGGCGCGGGTGGCACGTTCTGCGCGGGCATGGACCTACGGGCCTTCAGCGCGCGCCCACCGGAAGAGGCCGCCGCT
GCGCTCGCCCGCCTGGTACGGCACTCGACCCGGAAGCCGCTGGTCGCCGCGATCGACGGATTCGCCGTCGGAGGCGGTCT
CGAACTCGCGCTTGCTTGTGACCTGATGGTCGCCACGCCGGACGCCAGGCTCGGAATTCCGGAGGTGGCCCGCGGCCTGG
TTCCCTCCGGTGGCGCCCTGCTCCGACTGCCCCATCGACTCCCGTACAACGTGGCCCTCGACATGGCCCTCACCGGTCAA
CCGATCTCCGGCATCCGTGCCCACGAGCTAGGCCTGGTGTCACGGCTGGCCGATCCCGGCGACCCGCTCGGCATGGGACT
TGCCGTCGCCGCCTCGATCGCCGCAGCGGGGCAACTCGCCGTCAACGGAAGCAAGACGATCATGAGTCGTCGCATCGACG
ACGCCGAGTCAGCCGAGGCGTGGGAGGCACAGTTCGCGGTCACCCTGGACACCAATGCCTCCGAGGACGCGCACGAGGGC
ATCAGGGCATTCCTGGAGAAGCGCGCCCCCGTGTTCCGCGGTCGCTGA

Upstream 100 bases:

>100_bases
CTCTGCCGCCGGCGTACGGGGCAGACACCGACACGGTTCTGGCCGAGATCGGCTACTCCCCCGAACGCATCGCCTGCTTG
CGCGAAGTGGGGGCCATCTC

Downstream 100 bases:

>100_bases
CCTGGGGCATGACCGGCCCGGGCCTAGGGCGACGGGGCGAACTGCGGGAGGATCTCGTGCGCGATGCGGTCGAGCATCAC
GTCAGCATCCTCTTGGGGTG

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTEEAVLTSQRDGVLVVTLNRPNMRNAINEELSLGVAEAMARLDQSDALRVAVLHGAGGTFCAGMDLRAFSARPPEEAAA
ALARLVRHSTRKPLVAAIDGFAVGGGLELALACDLMVATPDARLGIPEVARGLVPSGGALLRLPHRLPYNVALDMALTGQ
PISGIRAHELGLVSRLADPGDPLGMGLAVAASIAAAGQLAVNGSKTIMSRRIDDAESAEAWEAQFAVTLDTNASEDAHEG
IRAFLEKRAPVFRGR

Sequences:

>Translated_255_residues
MTEEAVLTSQRDGVLVVTLNRPNMRNAINEELSLGVAEAMARLDQSDALRVAVLHGAGGTFCAGMDLRAFSARPPEEAAA
ALARLVRHSTRKPLVAAIDGFAVGGGLELALACDLMVATPDARLGIPEVARGLVPSGGALLRLPHRLPYNVALDMALTGQ
PISGIRAHELGLVSRLADPGDPLGMGLAVAASIAAAGQLAVNGSKTIMSRRIDDAESAEAWEAQFAVTLDTNASEDAHEG
IRAFLEKRAPVFRGR
>Mature_254_residues
TEEAVLTSQRDGVLVVTLNRPNMRNAINEELSLGVAEAMARLDQSDALRVAVLHGAGGTFCAGMDLRAFSARPPEEAAAA
LARLVRHSTRKPLVAAIDGFAVGGGLELALACDLMVATPDARLGIPEVARGLVPSGGALLRLPHRLPYNVALDMALTGQP
ISGIRAHELGLVSRLADPGDPLGMGLAVAASIAAAGQLAVNGSKTIMSRRIDDAESAEAWEAQFAVTLDTNASEDAHEGI
RAFLEKRAPVFRGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=270, Percent_Identity=30, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI20127408, Length=209, Percent_Identity=35.8851674641148, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI70995211, Length=248, Percent_Identity=32.6612903225806, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI37594469, Length=186, Percent_Identity=31.1827956989247, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI37594471, Length=186, Percent_Identity=31.1827956989247, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=29.6153846153846, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=29.8804780876494, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI157694516, Length=181, Percent_Identity=27.0718232044199, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI213417737, Length=181, Percent_Identity=27.0718232044199, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=182, Percent_Identity=29.1208791208791, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=182, Percent_Identity=29.1208791208791, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260274832, Length=182, Percent_Identity=29.1208791208791, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=185, Percent_Identity=24.8648648648649, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI221136753, Length=185, Percent_Identity=24.8648648648649, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI221307494, Length=185, Percent_Identity=24.8648648648649, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=35.5212355212355, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=34.7169811320755, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=30.188679245283, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788682, Length=183, Percent_Identity=34.9726775956284, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790281, Length=171, Percent_Identity=29.2397660818713, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=23.1939163498099, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=23.9382239382239, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=31.6205533596838, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=33.4677419354839, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=29.0836653386454, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=191, Percent_Identity=34.5549738219895, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=31.7073170731707, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=215, Percent_Identity=30.2325581395349, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=219, Percent_Identity=34.2465753424658, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=219, Percent_Identity=34.2465753424658, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=219, Percent_Identity=34.2465753424658, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=180, Percent_Identity=30.5555555555556, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=180, Percent_Identity=30.5555555555556, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=219, Percent_Identity=26.9406392694064, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=271, Percent_Identity=24.7232472324723, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=189, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357171, Length=253, Percent_Identity=30.8300395256917, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24650670, Length=198, Percent_Identity=37.8787878787879, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=30.4878048780488, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=30.4878048780488, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19921000, Length=228, Percent_Identity=37.719298245614, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24583077, Length=232, Percent_Identity=37.5, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24583079, Length=232, Percent_Identity=37.5, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922422, Length=257, Percent_Identity=25.6809338521401, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920382, Length=266, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571730, Length=185, Percent_Identity=29.1891891891892, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571729, Length=185, Percent_Identity=29.1891891891892, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24654903, Length=182, Percent_Identity=25.8241758241758, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26626; Mature: 26495

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEAVLTSQRDGVLVVTLNRPNMRNAINEELSLGVAEAMARLDQSDALRVAVLHGAGGT
CCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FCAGMDLRAFSARPPEEAAAALARLVRHSTRKPLVAAIDGFAVGGGLELALACDLMVATP
EEECCCCEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEEEEEEEEEECC
DARLGIPEVARGLVPSGGALLRLPHRLPYNVALDMALTGQPISGIRAHELGLVSRLADPG
CCCCCCHHHHHCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCC
DPLGMGLAVAASIAAAGQLAVNGSKTIMSRRIDDAESAEAWEAQFAVTLDTNASEDAHEG
CCHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCCCCCCHHCEEEEEEECCCCCCHHHHH
IRAFLEKRAPVFRGR
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEEAVLTSQRDGVLVVTLNRPNMRNAINEELSLGVAEAMARLDQSDALRVAVLHGAGGT
CCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FCAGMDLRAFSARPPEEAAAALARLVRHSTRKPLVAAIDGFAVGGGLELALACDLMVATP
EEECCCCEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEEEEEEEEEECC
DARLGIPEVARGLVPSGGALLRLPHRLPYNVALDMALTGQPISGIRAHELGLVSRLADPG
CCCCCCHHHHHCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCC
DPLGMGLAVAASIAAAGQLAVNGSKTIMSRRIDDAESAEAWEAQFAVTLDTNASEDAHEG
CCHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCCCCCCHHCEEEEEEECCCCCCHHHHH
IRAFLEKRAPVFRGR
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA