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The map label for this gene is phrA [H]

Identifier: 119715025

GI number: 119715025

Start: 822258

End: 823619

Strand: Reverse

Name: phrA [H]

Synonym: Noca_0778

Alternate gene names: 119715025

Gene position: 823619-822258 (Counterclockwise)

Preceding gene: 119715028

Following gene: 119715023

Centisome position: 16.52

GC content: 76.36

Gene sequence:

>1362_bases
ATGCCACCCTCCACGCCTGCGGTCCTGTGGTTCCGCCGCGATCTGCGCCTGGCCGACAACCCCGCGCTCGTCGAGGCGGC
CGCCGACGGACCGGTGCTGCCGCTGTTCGTCCTCGACCCGGTGCTCTGGGGGCCGGCCGGGGCCGCTCGCCGGGCCTATC
TGGGCGCGTCCCTGCGCGCGCTCGACGCCTCGCTGCGCGAGCGCGGCACCCGGCTGTCGGTCGTTCGCGGCGACCCGGCG
CGCCTGGTCCCGCGCGCCGCCCGGGCGGTCGGCGCGGCGCGGGTGCACGTCGCGGCGGACTTCGGTCCCTACGGGAGCCG
GCGCGACGGCGCGGTCGAGCAGGCGCTCACCGGCGCCGGGGCCGAGCTGGTCCGCACGGGCTCGCCGTACGCGGTGGCAC
CGGGGCGGGTGACCAACACCGCGGGCGCGCCGTACCGCGTCTTCACCCCGTTCCACCGCGCCTGGGCCGAGCACGGCTGG
CGCGGCCCGGTCGGCGTGCCCGAGGGCACCGGGTGGCTCCGACTGGCGGAGGGCACGGCCGAGATCCCCGACCCCGCGCT
GCCGGACGGGCTGACCCTCCCCGCGGCCGGTGAGGCCGCGGCGCGGCGTCGGTGGGCGGAGTTCTTGGACCGGGTGGACG
AGTACGCCGAGGACCGCGACCGGCCGGCCGTGGACGGCACCTCGCAGATGTCGGCGCACCTGAAGTGGGGTGAGATCCAT
CCGCGCACGATGCTCGCCGACCTGGCCCGGCGGCGCGGCGCCGGCGCGGCGACGTACCGCAAGGAGCTGGCCTGGCGGGA
GTTCTACGCCGACGTGCTGCACGCCCGTCCCGAGACCGCGCGGCAGTACCTGCGACCCGAGTTCGCCCGAATGCGGTACG
ACGAGCCGGGTGAGCACCTCGACGCGTGGCGACACGGCCGCACGGGGTTCTCGGTGGTGGACGCCGGGATGCGCCAGCTG
CGCGCGACCGGCTGGATGCACAACCGGGTCCGGATGATCACCGCGAGCTTCCTGGTCAAGGACCTGCACCTGGAATGGCA
GCTGGGCGCCCGGCACTTCCTGCGCTGGCTGGTCGACGGCGACCTGGCGTCCAACCAGCACGGCTGGCAGTGGACCGCGG
GGTGCGGCACCGACGCCGCGCCGTACTTCCGGGTGTTCAACCCGACGGCGCAGGGGCGTCGGTTCGACCCTCGTGGCGAG
TACGTGCGCCGGTGGGTGCCGGAGCTCGCGGACCCCGACGTGGTCGCCGACCCGCACGACCCGAGTCCTTCGGAGCGGCA
GGCGGTCGGCTACCCCGAGCCGATCGTCGACCACGCGGCCGAGCGCCGCGAGGCGCTCGGCCGGTGGGAGCGGATCCGCT
GA

Upstream 100 bases:

>100_bases
AGCTCGTAGATGGTCCGGAGGTACATCTCGGTGGTGTCGATGAGATCGCTCACAGCTGCATTGTCCCCTATCGACCCACC
CCCTGGCAGGGTGGACGCCG

Downstream 100 bases:

>100_bases
CCCGGCCCCGCTACGACAGCCGGTCGAGGTCGTAGAGGTACAGGTCGGGCCGCCCGTCGGTGTCGGCGGGGTCGACCGCG
ACGTTGGTCAACACCGACGC

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MPPSTPAVLWFRRDLRLADNPALVEAAADGPVLPLFVLDPVLWGPAGAARRAYLGASLRALDASLRERGTRLSVVRGDPA
RLVPRAARAVGAARVHVAADFGPYGSRRDGAVEQALTGAGAELVRTGSPYAVAPGRVTNTAGAPYRVFTPFHRAWAEHGW
RGPVGVPEGTGWLRLAEGTAEIPDPALPDGLTLPAAGEAAARRRWAEFLDRVDEYAEDRDRPAVDGTSQMSAHLKWGEIH
PRTMLADLARRRGAGAATYRKELAWREFYADVLHARPETARQYLRPEFARMRYDEPGEHLDAWRHGRTGFSVVDAGMRQL
RATGWMHNRVRMITASFLVKDLHLEWQLGARHFLRWLVDGDLASNQHGWQWTAGCGTDAAPYFRVFNPTAQGRRFDPRGE
YVRRWVPELADPDVVADPHDPSPSERQAVGYPEPIVDHAAERREALGRWERIR

Sequences:

>Translated_453_residues
MPPSTPAVLWFRRDLRLADNPALVEAAADGPVLPLFVLDPVLWGPAGAARRAYLGASLRALDASLRERGTRLSVVRGDPA
RLVPRAARAVGAARVHVAADFGPYGSRRDGAVEQALTGAGAELVRTGSPYAVAPGRVTNTAGAPYRVFTPFHRAWAEHGW
RGPVGVPEGTGWLRLAEGTAEIPDPALPDGLTLPAAGEAAARRRWAEFLDRVDEYAEDRDRPAVDGTSQMSAHLKWGEIH
PRTMLADLARRRGAGAATYRKELAWREFYADVLHARPETARQYLRPEFARMRYDEPGEHLDAWRHGRTGFSVVDAGMRQL
RATGWMHNRVRMITASFLVKDLHLEWQLGARHFLRWLVDGDLASNQHGWQWTAGCGTDAAPYFRVFNPTAQGRRFDPRGE
YVRRWVPELADPDVVADPHDPSPSERQAVGYPEPIVDHAAERREALGRWERIR
>Mature_452_residues
PPSTPAVLWFRRDLRLADNPALVEAAADGPVLPLFVLDPVLWGPAGAARRAYLGASLRALDASLRERGTRLSVVRGDPAR
LVPRAARAVGAARVHVAADFGPYGSRRDGAVEQALTGAGAELVRTGSPYAVAPGRVTNTAGAPYRVFTPFHRAWAEHGWR
GPVGVPEGTGWLRLAEGTAEIPDPALPDGLTLPAAGEAAARRRWAEFLDRVDEYAEDRDRPAVDGTSQMSAHLKWGEIHP
RTMLADLARRRGAGAATYRKELAWREFYADVLHARPETARQYLRPEFARMRYDEPGEHLDAWRHGRTGFSVVDAGMRQLR
ATGWMHNRVRMITASFLVKDLHLEWQLGARHFLRWLVDGDLASNQHGWQWTAGCGTDAAPYFRVFNPTAQGRRFDPRGEY
VRRWVPELADPDVVADPHDPSPSERQAVGYPEPIVDHAAERREALGRWERIR

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=492, Percent_Identity=31.0975609756098, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI4758072, Length=488, Percent_Identity=29.9180327868852, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI188536103, Length=447, Percent_Identity=29.7539149888143, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1786926, Length=475, Percent_Identity=36.6315789473684, Blast_Score=276, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6324962, Length=499, Percent_Identity=27.8557114228457, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17137248, Length=489, Percent_Identity=32.3108384458078, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24585455, Length=489, Percent_Identity=32.3108384458078, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24648152, Length=501, Percent_Identity=28.5429141716567, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 50364; Mature: 50233

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSTPAVLWFRRDLRLADNPALVEAAADGPVLPLFVLDPVLWGPAGAARRAYLGASLRA
CCCCCCEEEEEECCCCCCCCCCEEEECCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHH
LDASLRERGTRLSVVRGDPARLVPRAARAVGAARVHVAADFGPYGSRRDGAVEQALTGAG
HHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHCCC
AELVRTGSPYAVAPGRVTNTAGAPYRVFTPFHRAWAEHGWRGPVGVPEGTGWLRLAEGTA
HHHHCCCCCCEECCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
EIPDPALPDGLTLPAAGEAAARRRWAEFLDRVDEYAEDRDRPAVDGTSQMSAHLKWGEIH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCC
PRTMLADLARRRGAGAATYRKELAWREFYADVLHARPETARQYLRPEFARMRYDEPGEHL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHCCCCCCHHHH
DAWRHGRTGFSVVDAGMRQLRATGWMHNRVRMITASFLVKDLHLEWQLGARHFLRWLVDG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLASNQHGWQWTAGCGTDAAPYFRVFNPTAQGRRFDPRGEYVRRWVPELADPDVVADPHD
CCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
PSPSERQAVGYPEPIVDHAAERREALGRWERIR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PPSTPAVLWFRRDLRLADNPALVEAAADGPVLPLFVLDPVLWGPAGAARRAYLGASLRA
CCCCCEEEEEECCCCCCCCCCEEEECCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHH
LDASLRERGTRLSVVRGDPARLVPRAARAVGAARVHVAADFGPYGSRRDGAVEQALTGAG
HHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHCCC
AELVRTGSPYAVAPGRVTNTAGAPYRVFTPFHRAWAEHGWRGPVGVPEGTGWLRLAEGTA
HHHHCCCCCCEECCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
EIPDPALPDGLTLPAAGEAAARRRWAEFLDRVDEYAEDRDRPAVDGTSQMSAHLKWGEIH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCC
PRTMLADLARRRGAGAATYRKELAWREFYADVLHARPETARQYLRPEFARMRYDEPGEHL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHCCCCCCHHHH
DAWRHGRTGFSVVDAGMRQLRATGWMHNRVRMITASFLVKDLHLEWQLGARHFLRWLVDG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLASNQHGWQWTAGCGTDAAPYFRVFNPTAQGRRFDPRGEYVRRWVPELADPDVVADPHD
CCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
PSPSERQAVGYPEPIVDHAAERREALGRWERIR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]