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The map label for this gene is cysB [H]

Identifier: 119714892

GI number: 119714892

Start: 683272

End: 684141

Strand: Reverse

Name: cysB [H]

Synonym: Noca_0644

Alternate gene names: 119714892

Gene position: 684141-683272 (Counterclockwise)

Preceding gene: 119714895

Following gene: 119714889

Centisome position: 13.72

GC content: 67.36

Gene sequence:

>870_bases
GTGCAGTTGCGCCACCTTCGATATTTCGTCGCGACTGCCGACGCCGGAAGCGTCAGCGCAGCCGCTGTGCGGGTGCACGT
GACGCAGCCAGCCCTGTCCCGTCAGCTCCGCCAGCTCGAGCAGGATCTGGGGGTAGCGCTGTTCGACCGAAGCGCCGGAC
GGCTGGTGCTCAGTCGTACCGGTCAGGCCCTGCTACCCGCCGCTCGCGAGTTGCTCGCCTCAGCCCATGCCCTCGAGGCG
CGAGCAACGTTCCATGCACGGGGCAGCCTGGAGCGCGTGACCATCGCGGCGCCGACGGTGACGCTCACCGACGTGGTGTC
GCCTTTCGTCGCCACCATGTCTAAAGATGATCCGGTCGTCGACGTCCGCAGTGCCGACGGCCTCTCCCCGGCGGAGATGC
TGCACGACGGGGCGGATCTAGCCATTGGAACTCAACGGCTAGATCCGCCCTATGCGATGCACGAGCTAGCAGTGTTGCCT
GTCTGGGCCTACGTGCGGGCAACCGACCCGTGGGCCAATCGTACTCGCGTCGCGCTCGACGAACTGCTTCAACGCCCGCT
CGTGGTCCTGCCATCCACGTTTACGGCTCGGGAAGCGCTGGACTCCGCAGTCTTCGCGCTTGGCGGCTCGTATAGCGCGG
TTGTCGAGGCGGCAAACGGAACGATCGCCCAGGCTCTGGCCGCGTCCGGCCGCGGGGTGGCGGTCGTGACCGACGACTCT
CGATTCGACCTGAGCCCGCTCTCCATCGAGCTCAGCGCAGGCGTATTGGAGATCCGGCTGACGGCTGCGTGGGACCCGCG
CGGAGTAGCCGCCGCCACGATTGCCGACCTCGCTCGACGCCTCGGCCTTTGGGTGCAGACCGCTTACTGA

Upstream 100 bases:

>100_bases
TCGGATACAGCAGTCATGCAGTCAGTTTCGCGACTAGGAAGTTATGCTGTCCAATACCTATTTCAGTCCATTCCCATGCC
CTGTCGACATAGGAGGCTAA

Downstream 100 bases:

>100_bases
GCAAAGAGCCCTCGACGTGTTAGTCCGGTGGTACGACGAAGCGCCCCACCGCCGACAGGCGGTCCGCTCTTCCCCGGAGA
TCTTTCTGCAGACGCTCAGC

Product: regulatory protein LysR

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MQLRHLRYFVATADAGSVSAAAVRVHVTQPALSRQLRQLEQDLGVALFDRSAGRLVLSRTGQALLPAARELLASAHALEA
RATFHARGSLERVTIAAPTVTLTDVVSPFVATMSKDDPVVDVRSADGLSPAEMLHDGADLAIGTQRLDPPYAMHELAVLP
VWAYVRATDPWANRTRVALDELLQRPLVVLPSTFTAREALDSAVFALGGSYSAVVEAANGTIAQALAASGRGVAVVTDDS
RFDLSPLSIELSAGVLEIRLTAAWDPRGVAAATIADLARRLGLWVQTAY

Sequences:

>Translated_289_residues
MQLRHLRYFVATADAGSVSAAAVRVHVTQPALSRQLRQLEQDLGVALFDRSAGRLVLSRTGQALLPAARELLASAHALEA
RATFHARGSLERVTIAAPTVTLTDVVSPFVATMSKDDPVVDVRSADGLSPAEMLHDGADLAIGTQRLDPPYAMHELAVLP
VWAYVRATDPWANRTRVALDELLQRPLVVLPSTFTAREALDSAVFALGGSYSAVVEAANGTIAQALAASGRGVAVVTDDS
RFDLSPLSIELSAGVLEIRLTAAWDPRGVAAATIADLARRLGLWVQTAY
>Mature_289_residues
MQLRHLRYFVATADAGSVSAAAVRVHVTQPALSRQLRQLEQDLGVALFDRSAGRLVLSRTGQALLPAARELLASAHALEA
RATFHARGSLERVTIAAPTVTLTDVVSPFVATMSKDDPVVDVRSADGLSPAEMLHDGADLAIGTQRLDPPYAMHELAVLP
VWAYVRATDPWANRTRVALDELLQRPLVVLPSTFTAREALDSAVFALGGSYSAVVEAANGTIAQALAASGRGVAVVTDDS
RFDLSPLSIELSAGVLEIRLTAAWDPRGVAAATIADLARRLGLWVQTAY

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=29.8804780876494, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=33.4782608695652, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367136, Length=244, Percent_Identity=26.2295081967213, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787879, Length=197, Percent_Identity=27.9187817258883, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=73, Percent_Identity=39.7260273972603, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=27.9835390946502, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=199, Percent_Identity=29.6482412060301, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081904, Length=248, Percent_Identity=29.4354838709677, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30690; Mature: 30690

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRHLRYFVATADAGSVSAAAVRVHVTQPALSRQLRQLEQDLGVALFDRSAGRLVLSRT
CCCCCEEEEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCEEEEECC
GQALLPAARELLASAHALEARATFHARGSLERVTIAAPTVTLTDVVSPFVATMSKDDPVV
CCHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCEEHHHHHHHHHHHCCCCCCEE
DVRSADGLSPAEMLHDGADLAIGTQRLDPPYAMHELAVLPVWAYVRATDPWANRTRVALD
EECCCCCCCHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
ELLQRPLVVLPSTFTAREALDSAVFALGGSYSAVVEAANGTIAQALAASGRGVAVVTDDS
HHHCCCEEEECCCHHHHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHCCCCEEEEEECCC
RFDLSPLSIELSAGVLEIRLTAAWDPRGVAAATIADLARRLGLWVQTAY
CCCCCCEEEEEECCEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECC
>Mature Secondary Structure
MQLRHLRYFVATADAGSVSAAAVRVHVTQPALSRQLRQLEQDLGVALFDRSAGRLVLSRT
CCCCCEEEEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCEEEEECC
GQALLPAARELLASAHALEARATFHARGSLERVTIAAPTVTLTDVVSPFVATMSKDDPVV
CCHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCEEHHHHHHHHHHHCCCCCCEE
DVRSADGLSPAEMLHDGADLAIGTQRLDPPYAMHELAVLPVWAYVRATDPWANRTRVALD
EECCCCCCCHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
ELLQRPLVVLPSTFTAREALDSAVFALGGSYSAVVEAANGTIAQALAASGRGVAVVTDDS
HHHCCCEEEECCCHHHHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHCCCCEEEEEECCC
RFDLSPLSIELSAGVLEIRLTAAWDPRGVAAATIADLARRLGLWVQTAY
CCCCCCEEEEEECCEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]